terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterization of bunch compactness and identification of associated genes in a diverse collection of cultivars of Vitis vinifera L.

Characterization of bunch compactness and identification of associated genes in a diverse collection of cultivars of Vitis vinifera L.

Abstract

Compactness is a complex trait of V. vinifera L. and is defined ultimately by the portion of free space within the bunch which is not occupied by the berries. A high degree of compactness results in poor ventilation and consequently a higher susceptibility to fungal diseases, diminishing the quality of the fruit. The easiness to conceptualize the trait and its importance arguably contrasts with the difficulty to measure and quantify it. However, recent technical advancements have allowed to study this attribute more accurately over the last decade. Our main objective was to explore the underlying genetics determining bunch compactness by applying updated phenotyping methods in a collection of V. vinifera L. cultivars with a wide genetic diversity.
A collection of 116 genotypes composed by 24 wine, 56 table and 26 mixed usage varieties was characterized over two seasons measuring several traits determining Compactness Indexes (CI), as proposed in literature. CI-18 performed the best on this collection and genotypes with extreme values with loose and dense bunches were further studied (n = 10 each group). RNA of these samples was collected at key developmental stages to study the expression of VvUCC1, VvGRF4 and other genes associated to this trait. We also evaluated the performance of automated phenotyping for this wide collection of varieties by applying precision phenotyping through 3D scan and point cloud library-based methods. Combining this data with 127,631 informative SNPs identified by genotyping-by-sequencing could lead to identify further loci associated with this attribute through GWAS.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marco Meneses1, Renato Fuentes1, Ignacia Fuentes1, Claudia Muñoz-Espinoza2, Carolina Araya1, Juan Iribarra1, Erika Salazar1, Claudio Meneses3, Katja Herzog4, Patricio Hinrichsen1*

1 Instituto de Investigaciones Agropecuarias, INIA La Platina. Santiago, Chile.
2 Agronomy Faculty, Universidad de Concepción. Chillán, Chile.
3 Agronomy Faculty, P. Universidad Católica de Chile. Santiago, Chile.
4 Julius Kühn-Institut. Institute for Grapevine Breeding. Geilweilerhof, Germany.

Contact the author*

Keywords

Bunch compactness, qPCR, GBS, automated phenotyping

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Multiple description and validation of autochthone grape varieties in the Carpathian Basin

Context and Purpose of the Study. In many countries, the preservation of grape varieties with heritage value is ensured by genebanks of outstanding significance, which allow for the study of these varieties and the assessment of their future roles in response to environmental, market, and social challenges.

Comparing different vineyard sampling densities and patterns for spatial interpolation of intrinsic water use efficiency

The need to rationalize agricultural inputs has recently increased interest in assessing vineyard variability in order to implement variable rate input applications, so-called ‘precision viticulture’. In many viticultural areas globally, precision viticulture is already widely used such as for selective harvesting and variable rate application (VRA) of inputs such as irrigation and/or fertilizer. Robust VRA relies on having a geostatistically accurate map (of one or more vineyard attributes) requiring high sampling densities, which can be cost- and time-prohibitive to obtain. Previous work on spatial interpolation using kriging have upscaled ground-based measurements, but such upscaling strategies are applicable only when vineyard conditions are spatially continuous and satisfies the assumption of second-order stationary processes. Alternatively, mixed models that combine kriging and auxiliary information, such as the regression kriging (RK) method, are more instructive for spatial predictions. In order to improve prediction accuracies, it is therefore necessary to incorporate additional information to achieve accurate spatial patterns with low error.

Influence of pre-harvest elicitors treatment during ripening period on phenolic composition in Monastrell grapes

Phenolic compounds are very importants in crop plants, which is why there have been the subject of a large number of studies

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Formation And Evolution Of Minty Terpenoids During Model Ageing Of Cabernet Franc And Merlot Wines

In recent years, a pool of terpenoids possibly implicated in minty odours and in the appreciable refreshing sensation, has been identified in long aged red Bordeaux wines (Lisanti et al., 2021, Picard et al., 2016; Picard et al., 2017). These compounds were found to play a key role in the so-called “ageing bouquet”, that can be defined as “the homogeneous, harmonious flavour resulting from the complex transformation process in wine during bottle storage” (Picard et al., 2015). Moreover the minty-fresh sensory dimension in fine aged red wines plays an important role in typicity judgement by wine professionals (Picard et al., 2015).