terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterization of bunch compactness and identification of associated genes in a diverse collection of cultivars of Vitis vinifera L.

Characterization of bunch compactness and identification of associated genes in a diverse collection of cultivars of Vitis vinifera L.

Abstract

Compactness is a complex trait of V. vinifera L. and is defined ultimately by the portion of free space within the bunch which is not occupied by the berries. A high degree of compactness results in poor ventilation and consequently a higher susceptibility to fungal diseases, diminishing the quality of the fruit. The easiness to conceptualize the trait and its importance arguably contrasts with the difficulty to measure and quantify it. However, recent technical advancements have allowed to study this attribute more accurately over the last decade. Our main objective was to explore the underlying genetics determining bunch compactness by applying updated phenotyping methods in a collection of V. vinifera L. cultivars with a wide genetic diversity.
A collection of 116 genotypes composed by 24 wine, 56 table and 26 mixed usage varieties was characterized over two seasons measuring several traits determining Compactness Indexes (CI), as proposed in literature. CI-18 performed the best on this collection and genotypes with extreme values with loose and dense bunches were further studied (n = 10 each group). RNA of these samples was collected at key developmental stages to study the expression of VvUCC1, VvGRF4 and other genes associated to this trait. We also evaluated the performance of automated phenotyping for this wide collection of varieties by applying precision phenotyping through 3D scan and point cloud library-based methods. Combining this data with 127,631 informative SNPs identified by genotyping-by-sequencing could lead to identify further loci associated with this attribute through GWAS.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marco Meneses1, Renato Fuentes1, Ignacia Fuentes1, Claudia Muñoz-Espinoza2, Carolina Araya1, Juan Iribarra1, Erika Salazar1, Claudio Meneses3, Katja Herzog4, Patricio Hinrichsen1*

1 Instituto de Investigaciones Agropecuarias, INIA La Platina. Santiago, Chile.
2 Agronomy Faculty, Universidad de Concepción. Chillán, Chile.
3 Agronomy Faculty, P. Universidad Católica de Chile. Santiago, Chile.
4 Julius Kühn-Institut. Institute for Grapevine Breeding. Geilweilerhof, Germany.

Contact the author*

Keywords

Bunch compactness, qPCR, GBS, automated phenotyping

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Towards stopping pesticides: survey identification of on-farm solutions

The winegrowing sector consumes a lot of pesticides. Changes in vineyard are necessary in order to reduce or even stop using pesticides, and thus limit their harmful impacts on health and on environment. To answer these issues, the VITAE project (2021-2026) aims at designing pesticide free grapevine systems in France. For that, we take an interest in the vineyards using solutions to strongly reduce chemicals but also biopesticides. We assume that such vineyards exist and that they are implementing solutions that could inspire the design of free- pesticide system.

Influence of oak species on the differentiation of aged brandies using chemometrics approach based on phenolic compounds UHPLC fingerprints

Oak is the main material used in cooperage for making barrels and wood chips destined to aged spirits and wines. Quercus alba L., Quercus petraea L. and Quercus robur L. are three of the most commonly used oak species in cooperage companies.

Deciphering grapevine trunk early molecular responses to P. minimum and P. chlamydospora in the presence of a commercial biocontrol agent (Trichoderma atroviride, Vintec®)

Esca, one of the main grapevine trunk diseases, is a complex and poorly understood disease. Phaeoacremonium minimum and Phaeomoniella chlamydospora, two of the main pathogens associated to this disease, are thought to be responsible for the first trunk infections. Little is known concerning grapevine trunk defenses during pathogen infection.

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Response to powdery and downy mildew of varieties with disease resistance genes (PIWI)

Erysiphe necator and Plasmopara viticola are the causal agents of powdery and downy mildew on grapevines, leading to significant economic losses. Numerous chemical treatments are applied to control these diseases, leading to environmental problems and the appearance of resistance to these products. Therefore, the study of new strategies to achieve the objectives of sustainable development is a priority. In this sense, the use of new varieties resistant to these diseases may be an option of interest. The objective of this work was to analyze the degree of resistance of 9 varieties with downy mildew resistance genes (Rpv3 and/or Rpv12), four of which also carry a powdery mildew resistance gene (Ren 1) by in vitro inoculation assays.