terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterization of bunch compactness and identification of associated genes in a diverse collection of cultivars of Vitis vinifera L.

Characterization of bunch compactness and identification of associated genes in a diverse collection of cultivars of Vitis vinifera L.

Abstract

Compactness is a complex trait of V. vinifera L. and is defined ultimately by the portion of free space within the bunch which is not occupied by the berries. A high degree of compactness results in poor ventilation and consequently a higher susceptibility to fungal diseases, diminishing the quality of the fruit. The easiness to conceptualize the trait and its importance arguably contrasts with the difficulty to measure and quantify it. However, recent technical advancements have allowed to study this attribute more accurately over the last decade. Our main objective was to explore the underlying genetics determining bunch compactness by applying updated phenotyping methods in a collection of V. vinifera L. cultivars with a wide genetic diversity.
A collection of 116 genotypes composed by 24 wine, 56 table and 26 mixed usage varieties was characterized over two seasons measuring several traits determining Compactness Indexes (CI), as proposed in literature. CI-18 performed the best on this collection and genotypes with extreme values with loose and dense bunches were further studied (n = 10 each group). RNA of these samples was collected at key developmental stages to study the expression of VvUCC1, VvGRF4 and other genes associated to this trait. We also evaluated the performance of automated phenotyping for this wide collection of varieties by applying precision phenotyping through 3D scan and point cloud library-based methods. Combining this data with 127,631 informative SNPs identified by genotyping-by-sequencing could lead to identify further loci associated with this attribute through GWAS.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marco Meneses1, Renato Fuentes1, Ignacia Fuentes1, Claudia Muñoz-Espinoza2, Carolina Araya1, Juan Iribarra1, Erika Salazar1, Claudio Meneses3, Katja Herzog4, Patricio Hinrichsen1*

1 Instituto de Investigaciones Agropecuarias, INIA La Platina. Santiago, Chile.
2 Agronomy Faculty, Universidad de Concepción. Chillán, Chile.
3 Agronomy Faculty, P. Universidad Católica de Chile. Santiago, Chile.
4 Julius Kühn-Institut. Institute for Grapevine Breeding. Geilweilerhof, Germany.

Contact the author*

Keywords

Bunch compactness, qPCR, GBS, automated phenotyping

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The start of Croatian grapevine breeding program

Modern viticulture in Croatia and the world is mainly based on the grapevine varieties susceptible to various diseases and pests, which leads to unsustainable use of large amounts of pesticides. The sustainable development of viticulture in the future will only be possible by increasing the resistance of the grapevine through the development of new resistant varieties. Breeding programs have been launched in the leading wine-growing countries with the aim of developing resistant varieties possessing high quality level. Coratia is rich in in native grapevine varieties that are the basis of wine production, and are not included in the breeding programs of other countries.

Peptides diversity and oxidative sensitivity: case of specific optimized inactivated yeasts

Estimation of the resistance of a wine against oxidation is of great importance for the wine. To that purpose, most of the commonly used chemical assays that are dedicated to estimate the antioxidant (or antiradical) capacity of a wine consist in measuring the capacity of the wine to reduce an oxidative compound or a stable radical.

Symbiotic microorganisms application in vineyards: impacts on grapevine performance and microbiome

Microorganism-based inoculants have been suggested as a viable solution to mitigate the adverse effects of climate change on viticulture. However, the actual effectiveness of these inoculants when applied under field conditions remains a challenge, and their effects on the existing soil microbiota are still uncertain. This study investigates the impact of arbuscular mycorrhizal fungi inoculation on grapevine performance and microbiome. The study was conducted in a vineyard of Callet cultivar in Binissalem, Mallorca, Spain. Two different treatments were applied: control and inoculation with commercial mycorrhizae complex of Rhizoglomus irregulare applied to plants through irrigation.

Factors involved in the acumulation of acetic acid inside the grapes during winemaking by carbonic maceration

Vinification by carbonic maceration (CM) is based in the anaerobic fermentative metabolism also called intracellular fermentation (IF).

Kinetic investigations of the sulfite addition on flavanols

Sulfonated monomeric and dimeric flavan-3-ols are recently discovered in wine and proved to have great importance in understanding wine chemistry and quality [1, 2].