terclim by ICS banner
IVES 9 IVES Conference Series 9 New biotechnological approaches for a comprehensive characterization of AGL11 and its molecular mechanism underlying seedlessness trait in table grape

New biotechnological approaches for a comprehensive characterization of AGL11 and its molecular mechanism underlying seedlessness trait in table grape

Abstract

In table grapes seedlessness is a crucial breeding target, mainly results from stenospermocarpy, linked to the Thompson Seedless variety. Several studies investigated the genetic control of seedlessness identifying AGL11, a MADS-box transcription factor, as a crucial gene.

We performed a deep investigation of the whole AGL11 gene sequence in a collection of grapevine varieties revealing three different promoter-CDS combinations. By investigating the expression of the three AGL11 alleles and evaluating their ability to activate the promoter region, we show that AGL11 regulates its transcription in a specific promoter-CDS manner. By a multi-AGL11 co-expression analysis we identified a methyl jasmonate esterase, an indole-3-acetate beta-glucosyltransferase, and an isoflavone reductase as top AGL11 candidate targets. In vivo experiments further confirmed AGL11 role in regulating these genes, demonstrating its significant influence in seed development and thus in seedlessness trait.

The overall data allowed us to propose a novel regulatory mechanism correlating AGL11 haplotype assortment and seedlessness class, suggesting potential applications in grapevine breeding for seedlessness and fruit size optimization.

With the aim to validate in planta the proposed regulatory mechanism, we are working to stable transform ‘microvine’ plants for producing a truncated or inactivated AGL11 protein. We first demonstrated the ability of embryogenic calli, obtained from ‘microvine’ anthers collection and culture, to regenerate embryos capable of germinating and sprouting into a new plant. The use of ‘microvine’ as a model system offers promising outcomes for functional gene characterization, benefiting viticulture genetic improvement and seedless table grape cultivation.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Alessandra Amato1*, Maria Francesca Cardone2, Oscar Bellon1, Lucia Rosaria Forleo2, Margherita D’Amico2, Carlo Bergamini2, Sara Zenoni1

1 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA), Turi, Bari, Italy

Contact the author*

Keywords

Table grape, Seedlessness, AGL11, Regulatory mechanism, Microvine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Nitrogen status of vines influences aged wines aromas. Examples of aged Champagne reserve wines and red Bordeaux wines

The sensory definition of the aging bouquet of red Bordeaux wines has been shown to be structured around seven main aromatic nuances: “undergrowth”, “spicy” “truffle”, “fresh red- and black-berry fruits”, “liquorice”, “mint”, and “toasted” (1). Some of these descriptors are also used to describe the aromatic notes of old Champagnes (2) suggesting common volatile compounds between these two types of wine.

Influence of two yeast strains and different nitrogen nutrition on the aromatic compounds in Lugana wine

Lugana Protected Designation of Origin (PDO) wines are made from Turbiana grapes. The aroma of Lugana wines results from the combined contribution of esters, terpenes, norisprenoids, sulfur compounds and the benzenoid methyl salicylate. This study aims to investigate how volatile aroma compounds are affected by different nitrogen supplies and yeast strains. Wines were produced with a standard protocol with 2021 Turbiana grapes with two different yeasts Zymaflore Delta e Zymaflore X5 (Laffort, France).During the alcoholic fermentation of the must, when H2S appeared, additions of various nitrogen supply were made: inorganic nitrogen, organic nitrogen, a mix of inorganic and organic nitrogen and organic nitrogen with an addition of pure methionine. During wine fermentation, a daily measurement of hydrogen sulfide was carried out.

The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

There is evidence in the literature that non-volatile wine matrix can modify the release and therefore the perception of the compounds involved in wine aroma [1-3].

Three new Apulian seedless varieties available for the table grape supply chain

The table grape industry in Europe is undergoing significant changes. For instance, in Italy, the national register of vine varieties was established in 1969, and since then, many varieties of table grapes have been registered. In the first thirty years, from 1969 to 1999, 62 seeded and 24 seedless varieties were registered. In the subsequent period, from 2000 to the present day, 23 seeded varieties and 99 seedless varieties were registered.

Variability of Tempranillo phenology within the toro do (Spain) and its relationship to climatic characteristics

Aims: The objective of this research was to analyse the spatial and temporal variability of vine phenology of the Tempranillo variety in the Toro Designation of Origen (DO) related to climatic conditions at present and under future climate change scenarios.