New biotechnological approaches for a comprehensive characterization of AGL11 and its molecular mechanism underlying seedlessness trait in table grape

Determining the age of a vineyard is essential for understanding its influence on wine quality and characteristics.

Currently, grapevine is host to a large number of pathogenic agents, including 65 viruses, five viroids and eight phytoplasmas. Needless to say, these pathogens, especially viruses responsible for several ‘infectious degeneration’ or ‘decline’ cause great distress to wine makers and grape growers, let alone the large economic losses incurred by the wine industry. A recent addition to this wide repertoire of grapevine viruses is a new viral disease known as Red Blotch in viticulture parlance. Its causal organism, Grapevine red blotch associated virus (GRBaV), discovered in 2008 is a newly identified virus of grapevines and a putative member of a new genus within the family Geminiviridae.

Synthetic pesticides are widely used in viticulture to ensure steady harvest quality and quantity. Fungicides are primarily used to control grapevine diseases but insecticides and herbicides are likewise used. Pesticide residues in viticultural areas currently represent a strong societal concern, but may also affect different trophic chains in such areas. In this project we wish to analyse honeybees collected from hives placed in different vineyards, their natural predator (the invasive hornet Vespa velutina), as well as the honey, grape berries, and wines produced.

A novel and efficient Dispersive Liquid-Liquid Microextraction (DLLME) method coupled with gas chromatography–mass spectrometry (GC–MS) was developed to determine 33 key aroma compounds (esters, alcohols, aldehydes, terpenes, norisoprenoids, fatty acids and phenols) present in Pinot noir (PN) wine. Four critical parameters including extraction solvent type, disperse solvent type, extraction solvent volume and disperse solvent volume were optimised with the aid of D-optimal design.

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.