terclim by ICS banner
IVES 9 IVES Conference Series 9 New biotechnological approaches for a comprehensive characterization of AGL11 and its molecular mechanism underlying seedlessness trait in table grape

New biotechnological approaches for a comprehensive characterization of AGL11 and its molecular mechanism underlying seedlessness trait in table grape

Abstract

In table grapes seedlessness is a crucial breeding target, mainly results from stenospermocarpy, linked to the Thompson Seedless variety. Several studies investigated the genetic control of seedlessness identifying AGL11, a MADS-box transcription factor, as a crucial gene.

We performed a deep investigation of the whole AGL11 gene sequence in a collection of grapevine varieties revealing three different promoter-CDS combinations. By investigating the expression of the three AGL11 alleles and evaluating their ability to activate the promoter region, we show that AGL11 regulates its transcription in a specific promoter-CDS manner. By a multi-AGL11 co-expression analysis we identified a methyl jasmonate esterase, an indole-3-acetate beta-glucosyltransferase, and an isoflavone reductase as top AGL11 candidate targets. In vivo experiments further confirmed AGL11 role in regulating these genes, demonstrating its significant influence in seed development and thus in seedlessness trait.

The overall data allowed us to propose a novel regulatory mechanism correlating AGL11 haplotype assortment and seedlessness class, suggesting potential applications in grapevine breeding for seedlessness and fruit size optimization.

With the aim to validate in planta the proposed regulatory mechanism, we are working to stable transform ‘microvine’ plants for producing a truncated or inactivated AGL11 protein. We first demonstrated the ability of embryogenic calli, obtained from ‘microvine’ anthers collection and culture, to regenerate embryos capable of germinating and sprouting into a new plant. The use of ‘microvine’ as a model system offers promising outcomes for functional gene characterization, benefiting viticulture genetic improvement and seedless table grape cultivation.

Download the abstract

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Alessandra Amato1*, Maria Francesca Cardone2, Oscar Bellon1, Lucia Rosaria Forleo2, Margherita D’Amico2, Carlo Bergamini2, Sara Zenoni1

1 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA), Turi, Bari, Italy

Contact the author*

Keywords

Table grape, Seedlessness, AGL11, Regulatory mechanism, Microvine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Contribution of seeds to red wine phenolic composition

Tannin composition is an important attribute in red wine quality, and it is therefore critical to understand the factors influencing tannin extraction during alcoholic fermentation. Tannins contribute to the mouthfeel of wines, but they also form pigmented polymers...
Read More

Simulating the impact of climate change on viticultural systems in various European vineyards

Aim: Global climate change affects regional climates and hold implications for wine growing regions worldwide (Jones, 2007, 2015; van Leeuwen and Darriet, 2016). The prospect of 21st century climate change consequently is one of the major challenges facing the wine industry (Keller, 2010).

Read More

Influence of viticulture on the temporary evolution of the landscape: the case of the AO Ribera del Duero (Central Spain)

The European Landscape Convention (ELC, 2001) defined the landscape as the “part of a
territory as perceived by the population and resulting from the action of natural and/or human factors and their interrelationships”. Wine landscapes, protected or not under figures such as cultural landscapes or Cultural heritage, are a clear demonstration of this definition, denoting the interrelationships of the natural
environment and the action of the human, which modulates the territory to give the different wine
landscapes. This work was focused on the study of the effect of the human factors linked to the cultivation of the vine on the modification of the landscape.

Read More

Prosensorial potential of new fungi-resistant varieties in modern oenology

The introduction into the Italian wine supply chain of the latest generation of fungi-resistant grapevine varieties, endowed with a greater or lesser strong resistance to downy and powdery mildews, represents a valid tool of making viticulture more sustainable, particularly in northern regions of the peninsula, where climatic conditions accentuate the pressure of fungal diseases. However, the affirmation of resistant varieties is a function of their agronomic value, as well as of their oenological and sensorial value. The purpose of this study was to evaluate in detail the sensory potential of the new resistant varieties, in order to understand their real possibility of inclusion in the modern global enological context.

Read More

Il piano regolatore delle citta’ del vino

Obiettivo generale di questo documenta è fornire un metodo di pianificazione che superi l’organizzazione delle aree rurali, ed in particolare vitate, finalizzata unicamente all’ot­timizzazione economico produttiva delle aziende, verso una pianificazione integrata degli spazi aperti.

Read More