terclim by ICS banner
IVES 9 IVES Conference Series 9 Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Abstract

The addition of partially dehydrated grapes to enrich must composition for producing complex dry/sweet wines represents a traditional practice in several regions of the world. However, the environmental conditions of dehydration chambers may facilitate the infection of Botrytis cinerea Pers. by promoting disease and provoking large grape losses. B. cinerea attack can induce alterations in the profile of volatile organic compounds (VOCs), which could be detected by sensors specifically trained to detect infection/disease-related compounds. These sensors could facilitate the early detection of the infection, consequently allowing to adjust some dehydration parameters. To deepen the understanding on alterations induced by B. cinerea on intact grape VOCs profile, berries from Sangiovese and Corvina cultivars were collected and analysed by SPME-GC-MS as such and following artificial inoculation with a spore suspension of B. cinerea (10 μl, 105 spores ml-1) or mock inoculation by using the same volume of sterile growth medium (control). Preliminary results have shown that high levels of a set of primary and secondary alcohols appeared to be emitted by inoculated berries. Some of these molecules are already reported as correlated with B. cinerea infection, while others are not mentioned as infection markers yet. Moreover, the dynamics of the emission of some of these compounds during the in vitro development of B. cinerea cultures have also been studied. Setting up sensors capable of detecting the identified volatile markers in the dehydration chambers represents an ambitious goal for reducing spoilage and grape losses via targeted interventions.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Pietro Emilio Nepi1*, Claudia Pisuttu2, Cristina Nali2, Elige Salame1, Pietro Tonutti1, Stefano Brizzolara1

1Crop Science Research Center, Scuola Superiore Sant’Anna di Studi Universitari, Piazza Martiri della Libertà,33,56127, PISA, ITALY
2Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80,56124, PISA, ITALY.(Left-aligned, italic, Arial 9)

Contact the author*

Keywords

grape, grey mould, VOCs, sensors, sustainability

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

WINE SWIRLING: A FIRST STEP TOWARDS THE UNLOCKING OF THE WINE’STASTER GESTURE

Right after the pouring of wine in a glass, a myriad of volatile organic compounds, including ethanol, overwhelm the glass headspace, thus causing the so-called wine’s bouquet [1]. Otherwise, it is worth noting that during wine tasting, most people automatically swirl their glass to enhance the release of aromas in the glass headspace [1]. About a decade ago, Swiss researchers revealed the complex fluid mechanics underlying wine swirling [2]. However, despite mechanically repeated throughout wine tasting, the consequences of glass swirling on the chemical space found in the headspace of wine glasses are still barely known.

Viticulture and climate: from global to local

Aims: This review aims to (1) present the multiple interests of studying and depicting and climate spatial variability for vitivinicultural terroirs study; (2) explain the factors that affect climate spatial variability according to the spatial scale considered and (3) provide guidelines for climate zoning considering challenges linked to each methodology considered.

Piloting grape ripening in a global warming scenario: feasible techniques are available

Under the pressure of global warming, several wine grape growing regions around the world are increasingly suffering from advanced and compressed phenology; endangering wine character while also creating serious logistic problems. From a physiological standpoint, the issue of delaying ripening is not simple as, in several instances, only a few processes must be delayed (i.e. sugar accumulation into the berries) while other events such as pigmentation and accumulation of other important phenolic compounds should proceed at a normal rate. Thus, the issue of decoupling technological maturity from phenolic maturity is another important consideration. Over the last decades, several research groups have endeavored to establish alternate cultural practices aimed at addressing this decoupling. In some cases, special applications of quite robust and well known practices regarding physiological principles have been utilized, however some completely new techniques are also being studied. In figure 1 of the review, we offer a panorama of the available tools and in the text we elaborate on those having provided most reliable and consistent results under an array of genotypes and environmental conditions. Among these, primary focus is given to post‐veraison—apical to the cluster—leaf removal (that can also be suitably replaced by applications of anti‐transpirants); the use of kaolin against multiple summers’ stresses; and a drastic version of late winter pruning having the potential to postpone ripening into a cooler period with improved grape composition and a limited negative impact on yield and storage reserves replenishment. 

Utilization of remote sensing technology to detect riesling vineyard variability

ineyard blocks can vary spatially with respect to several viticulturally significant qualities such as soil variables, vine vigor, vine physiology

Effects of progeny in the modulation of the response to water stress in isohydric and anisohydric varieties

Each grapevine variety has a specific water use regulation response under drought, and it is still unclear whether this regulation results from innate genotypic behavior (iso- and anisohydric), or is a response to environmental factors, namely recurrent water stress priming effects. In the present work, we explored the influence of the field-grown genotypes’ drought memory in the drought-response phenotype of their vegetative progenies, in Trincadeira (isohydric) and Castelão (anisohydric) varieties under a drought event followed by recovery in a glasshouse. Cuttings from both cultivars subjected to full irrigation (FI) and non-irrigation (NI) treatments for 5 consecutive years were used.