terclim by ICS banner
IVES 9 IVES Conference Series 9 Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Abstract

The addition of partially dehydrated grapes to enrich must composition for producing complex dry/sweet wines represents a traditional practice in several regions of the world. However, the environmental conditions of dehydration chambers may facilitate the infection of Botrytis cinerea Pers. by promoting disease and provoking large grape losses. B. cinerea attack can induce alterations in the profile of volatile organic compounds (VOCs), which could be detected by sensors specifically trained to detect infection/disease-related compounds. These sensors could facilitate the early detection of the infection, consequently allowing to adjust some dehydration parameters. To deepen the understanding on alterations induced by B. cinerea on intact grape VOCs profile, berries from Sangiovese and Corvina cultivars were collected and analysed by SPME-GC-MS as such and following artificial inoculation with a spore suspension of B. cinerea (10 μl, 105 spores ml-1) or mock inoculation by using the same volume of sterile growth medium (control). Preliminary results have shown that high levels of a set of primary and secondary alcohols appeared to be emitted by inoculated berries. Some of these molecules are already reported as correlated with B. cinerea infection, while others are not mentioned as infection markers yet. Moreover, the dynamics of the emission of some of these compounds during the in vitro development of B. cinerea cultures have also been studied. Setting up sensors capable of detecting the identified volatile markers in the dehydration chambers represents an ambitious goal for reducing spoilage and grape losses via targeted interventions.

Download the abstract

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Pietro Emilio Nepi1*, Claudia Pisuttu2, Cristina Nali2, Elige Salame1, Pietro Tonutti1, Stefano Brizzolara1

1Crop Science Research Center, Scuola Superiore Sant’Anna di Studi Universitari, Piazza Martiri della Libertà,33,56127, PISA, ITALY
2Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80,56124, PISA, ITALY.(Left-aligned, italic, Arial 9)

Contact the author*

Keywords

grape, grey mould, VOCs, sensors, sustainability

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Geologic and geomorphologic features applied for identification of wine terroir units by digital image processing, spectroradiometric and GIS techniques in Encruzilhada do Sul, RS, Brazil

Results in the characterization of a new wine terroir unit in south Brazil are reported. Presently, several areas in Brazil are being studied, in an effort to define new wine terroirs and improve the quality of Brazilian wines.

Read More

Geographical indication “Brandy Italiano”: study on the influence of wood barrel toasting and natural seasoning on endogenous and wood-derived compounds of aged distillates

The European geographical indication (GI) Brandy Italiano is exclusively reserved to brandy obtained in Italy from the distillation of wine from grapes grown and vinified in the national territory [1].

Read More

Influence of a spontaneous cover crop on the vineyard and soil erosion under Mediterranean climate

Sixty five % of the agricultural area of the Basque Country located in the DO Ca Rioja corresponds to vineyards. More than 40% of it has an average slope greater than 10%, which makes it sensitive to erosive processes. Furthermore, it is foreseeable that extreme weather events (storms, hail, extreme heat and cold, etc.) will be favored due to climate change. Cover cropping can mitigate this risk, and therefore the objective of this work is to evaluate the impact that a vegetable cover has on the agronomic behavior of the vineyard, the quality of the grape and soil erosion. For this, a trial has been carried out with a Graciano variety vineyard with a slope between 10% -20% during the years 2020 and 2021. Conventional tillage management in the area has been compared (4-6 passes per year of tillage machinery) versus spontaneous vegetation cover management in the vineyard. This implies not tilling and allowing the grass of the land to colonize the range between the lines of vines, controlling their height through 1-3 mowing passes per year, always trying to affect the surface of the land as little as possible. The vegetative growth, yield and quality of the grape and wine was measured. Furthermore, erosion has been measured using Gerlasch boxes. The yield was lower in the second year of the trial in the cover crop treatment, but erosion was significantly reduced.

Read More

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock.

Read More

Basic Terroir Unit (U.T.B.) and quality control label for honey; making the designations of origin (A.O.C) and« crus » more coherent

Considérant d’une part la judicieuse mise au point d’un label de qualité contrôlée des miels suisses (STÖCKLI et al. 1997), considérant d’autre part l’élaboration d’une carte des paysages végétaux (HEGG et al. 1993),

Read More