Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Over the past few decades, viticultural research has made numerous contributions which have made it possible to better understand the behavior of the vine as well as its response to the conditions imposed on it by the environment and agronomic practices. However, these results have only rarely been used in the practical management of vineyards because the research has been carried out using partial experimental models where reality is only represented by a few factors which are sometimes even made more complex by the introduction of elements foreign to the existing situation and difficult to apply to production (varieties, methods of cultivation, management techniques, etc.). To these reasons, one could add a low popularization of the results obtained, as well as the difficulty of implementing the scientific contributions, which does not allow the different production systems to fully express their potential. This limit of viticultural research can only be exceeded by the design of integrated projects designed directly on and for the territory. Indeed, only the integrated evaluation of a viticultural agro-system, which can be achieved through zoning, makes it possible to measure, or even attribute to each element of the system, the weight it exerts on the quality of the wine.

The volatile composition of grapes (free and bound forms) contributes greatly to the varietal aroma and quality of wines. Several agronomical parameters affect grapes composition and wine quality: maturity level at harvest, water status, and the intensity of sun exposure.

Three compounds, 3-mercaptohexanol (3MH), 3-mercaptohexyl-acetate (3MHA) and 4-mercapto-4-methylpentan-2-one (4MMP), also known as varietal thiols, have been identified to contribute positively to wine aroma and are responsible for the distinct gooseberry, grapefruit, guava and box tree character found in Sauvignon blanc wines. Certain volatile thiol compounds though, can cause off-aromas of onion, garlic, rubber and rotten egg, this group of molecules is known as reductive sulphur compounds (RSC). This study looks into how the addition of sulphur-compounds to Sauvignon blanc juice contributes to the varietal thiol (3MH and 3MHA) concentration and reductive sulphur compound concentration in South African Sauvignon blanc wine.

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA).

There is an urgent need to develop protective measures for grapevines in historically significant dry-farming vineyards, particularly on Mediterranean islands.