Terroir 2020 banner
IVES 9 IVES Conference Series 9 The dynamics of δ13C and δ18O in musts during berries development

The dynamics of δ13C and δ18O in musts during berries development

Abstract

Aim: Many processes or reactions that occur in plants involved isotopic discrimination. Water availability, for example, affects the isotopic ratio of carbon (δ13C) and oxygen (δ18O). In viticulture, δ13C is used in experiments related to water relations and irrigation in vineyards. δ18O is used much less but it could be a good complement to δ13C. The aim of this study was to generate knowledge on how these isotopic ratios, measured in musts, could help to better understand the water behavior of grape varieties. 

Methods and Results: The present work was carried out in 2019 with a set of seven varieties selected to monitor the aforementioned ratios in musts obtained during berries development: three of them red (Bobal, Tinto Velasco and Syrah), managed with irrigation, and the other three white (Airén, Malvar and Albillo Real) kept without irrigation; the seventh, Tempranillo, was managed with or without irrigation. 

Monitoring the dynamics of isotope ratios was undertaken through sampling of grapes carried out periodically, from shortly before closing cluster to maturity. In obtained musts, δ13C and δ18O were determined by mass spectrometry of isotope ratios.

The small changes observed between samples in δ13C in a specific variety did not seem to follow any pattern. In most cases, the comparisons of means performed showed no significant differences between samples. However, differences were observed between the two management systems: irrigated and rainfed; in dry conditions, with the stomatal closure, 13C isotopic discrimination declined during photosynthesis, and the ratio then increased.

This was not so with δ18O, where the comparisons of means always showed significant differences between samples. Dynamics of δ18O seemed to adapt, in this case, to a double curve pattern (cubic polynomial): the intense increase in the ratio of the first stages of fruit development was followed by a phase of slight decline, which lasted up to 15 or 20 days before harvest, at which point the ratio increased again. There were both inter-varietal and between management system differences: musts in early harvest varieties showed higher δ18O values than late varieties, while the isotopic enrichment was lower for this isotopic ratio in irrigated vines.

Conclusions:

Differences in the narrow margin in which δ13C values of the grapes are maintained throughout their development seemed to respond more to the crop management practice than to the variety. However, the notable changes in δ18O values seem to be due to a complex mechanism that involves the discharge of water in the grapes from the phloem at beginning of ripening and the loss of water due to transpiration through the skin.

Significance and Impact of the Study: In the search for the genotypes with the highest water efficiency that effectively respond to the proliferation and dilation of drought periods that are expected in many regions, it is urgent to explore the existing genetic variability. In this sense, δ13C and δ18O could be useful tools to take into account in any research related to water use by cultivars at physiological or agronomic levels.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Adelaida Mena Morales*, Juan Luis Chacón Vozmediano, Rosa Mª Sánchez-Gil Jimeno, Jesús Martínez Gascueña

Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de Castilla-La Mancha (IRIAF), IVICAM, Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Ciudad Real, Spain

Contact the author

Keywords

Grapevine, genotypes, musts, δ13C dynamics, δ18O dynamics, IRMS

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Local ancient grapevine cultivars to face future viticulture

Among the different strategies to cope with the negative impacts of climate change on viticulture, the exploitation of genetic diversity is one of the most promising to adapt to new conditions and maintain wine production and quality. One of the biggest concerns in the context of climate change is to improve water use efficiency (WUE). In this way, the use of genotypes that present a better response to drought and high WUE is a key issue. In this work, physiological performance analysis was conducted to compare the water deficit stress (WDS) responses of local and widespread grapevines cultivars. Leaf gas exchange, water use efficiency (WUE) at different levels (leaf and long-term WUE (∆13C)), leaf osmotic adjustment and other water relations parameters were determined in plants under well-watered and WDS conditions alongside assessment of the levels of foliar hormones concentrations. Results denote that local cultivars displayed better physiological performance under WDS as compared to the widely-distributed ones. he results corroborate the hypothesis that better stomatal control allows increasing leaf WUE under drought as occurred in the local Callet cv.; but the minority local cultivar Escursac cv. showed high WUE under both treatments. In this case, high WUE can be related to maintaining higher photosynthetic activity under drought. The different mechanisms underlying the better performance under WDS and high WUE of minority local cultivars are discussed.

A blueprint for managing vine physiological balance at different spatial and temporal scales in Champagne

In Champagne, the vine adaptation to different climatic and technical changes during these last 20 years can be seen through physiological balance disruptions. These disruptions emphasize the general grapevine decline. Since the 2000s, among other nitrogen stress indicators, the must nitrogen has been decreasing. The combination of restricted mineral fertilizers and herbicide use, the growing variability of spring rainfall, the increasing thermal stress as well as the soil type heterogeneity are only a few underlying factors that trigger loss of physiological balance in the vineyards. It is important to weigh and quantify the impact of these factors on the vine. In order to do so, the Comité Champagne uses two key-tools: networking and modelization. The use of quantitative and harmonized ecophysiological indicators is necessary, especially in large spatial scales such as the Champagne appellation. A working group with different professional structures of Champagne has been launched by the Comité Champagne in order to create a common ecophysiology protocol and thus monitor the vine physiology, yearly, around 100 plots, with various cultural practices and types of soil. The use of crop modelling to follow the vine physiological balance within different pedoclimatic conditions enables to understand the present balance but also predict the possible disruptions to come in future climatic scenarios. The physiological references created each year through the working group, benefit the calibration of the STICS model used in Champagne. In return, the model delivers ecophysiology indicators, on a daily scale and can be used on very different types of soils. This study will present the bottom-up method used to give accurate information on the impacts of soil, climate and cultural practices on vine physiology.

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.

Vineyards and clay minerals: multi-technique analytical approach and correlations with soil properties

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.