Terroir 2020 banner
IVES 9 IVES Conference Series 9 Soil microbial and arthropod biodiversity under organic and biodynamic viticulture

Soil microbial and arthropod biodiversity under organic and biodynamic viticulture

Abstract

Aims: The aim of the study was to investigate whether organic or biodynamic management have a long-term impact on 1) the microbial biomass and enzymatic activity in the soil, 2) the soil microbial community, 3) flying as well as soil living arthropods and associated fungi.

Methods and Results: The studies presented here were conducted in a field trial comparing integrated, organic and biodynamic viticulture at least 10 years after the implementation of the different management systems. The vineyard is located in Geisenheim, Germany, and the study is conducted on Vitis vinifera L. cv. Riesling.

One study assessed soil enzymatic activities (GLU, CAT, UR, DHA, PHO) and microbial biomass by quantifying PLFAs and NLFAs, respectively. For the second study soil fungal and bacterial biodiversity were investigated using an amplicon sequencing approach. For the third study eDNA was extracted from arthropods in bulk and soil samples. A DNA metabarcoding approach was used to investigate whether diversity of arthropods and fungi in these samples was affected by the management system.

Fungal and bacterial biomass as well as enzymatic activities in the soil were shown to be highly affected by the management system. The organic and the biodynamic systems had significantly more fungal and bacterial biomass. In contrast, the integrated system had a significantly higher mycorrhizal biomass compared to the organic and the biodynamic system. Enzymatic activities measured were significantly higher under organic and biodynamic management.

Fungal species richness assessed by DNA sequencing did not differ among management systems, but fungal community composition was significantly affected. Bacterial species richness was significantly higher under organic and biodynamic management, whereas bacterial community composition was less affected by the management system.

Richness of flying and soil-living arthropods and their related fungi assessed by eDNA sequencing was not significantly affected by the management system alone. In contrast, management systems significantly differed in the arthropod community composition in bulk samples as well as in fungal community composition associated with flying as well as soil-living arthropods.

Conclusions:

Different management systems have a clear impact on soil microbial activity, biomass, and biodiversity, as well as on arthropod biodiversity and fungal biodiversity associated with arthropods. In the current studies soil enzymatic activities as well as soil microbial biomass and bacterial species richness in the soil were positively affected by organic and biodynamic management. Fungal community composition in the soil, in samples of soil-living as well as in samples of flying arthropods were highly affected by the management system. The hypothesis of whether arthropods in the vineyard act as vectors for bacteria and fungi will be discussed.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type: Video

Authors

Johanna Döring1*, Matthias Friedel1, Jacob Agerbo Rasmussen3,4,5, Maximilian Hendgen2, Sofia Di Giacinto2, Randolf Kauer1

1Department of General and Organic Viticulture, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany
2Department of Soil Science and Plant Nutrition, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany
3Section for Evolutionary Genomics, The GLOBE Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Øster Farimagsgade 5A, 1352 Copenhagen, Denmark
4Biological Institute, Genome Research and Molecular Biomedicine, University of Copenhagen, Universitetsparken 13, 2200 Copenhagen, Denmark 
5Center for Evolutionary Hologenomics, University of Copenhagen, Øster Farimagsgade  5A,  1352 Copenhagen, Denmark

Contact the author

Keywords

Organic, biodynamic, soil microbial activity, soil microbial biomass, microbial biodiversity, arthropod biodiversity

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Pure wine vs natural wine

S’il n’existe pas de réglementation officielle, la démarche des vins naturels prône un retour aux pratiques dites ancestrales préconisant notamment un mode d’élaboration des vins utilisant le moins d’intrants possible. Le seul autorisé reste l’anhydride sulfureux (SO2) à des doses quatre à cinq fois moins importantes que pour les vins dits conventionnels. Ce désir de renouer avec

FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.

VINIoT – Precision viticulture service

The project VINIoT pursues the creation of a new technological vineyard monitoring service, which will allow companies in the wine sector in the SUDOE space to monitor plantations in real time and remotely at various levels of precision. The system is based on spectral images and an IoT architecture that allows assessing parameters of interest viticulture and the collection of data at a precise scale (level of grape, plant, plot or vineyard) will be designed. In France, three subjects were specifically developed: evaluation of maturity, of water stress, and detection of flavescence dorée. For the evaluation of maturity, it has been decided first to work at the berry scale in the laboratory, then at the bunch scale and finally in the vineyard. The acquisition of the spectral hyperstal image as well as the reference analyzes to measure the maturity, were carried out in the laboratory after harvesting the berries in a maturity monitoring context. This work focuses on a case study to predict sugar content of three different grape varieties: Syrah, Fer Servadou and Mauzac. A robust method called Roboost-PLSR, developed in the framework of this work (Courand et al., 2022), to improve prediction model performance was applied on spectra after the acquirement of hyperspectral images. Regarding the evaluation of water stress, to work with a significant variability in terms of water status, it has been worked first with potted plants under 2 different water regimes. The facilities have allowed the supervision of irrigation and micro-climatic conditions. The regression models on agronomic variables (stomatal conductance, water potential, …) are studied. To detect flavescence dorée, the experimental plan has consisted of work at leaf scale in the laboratory first, and then in the field. To detect the disease from hyper-spectral imaging, a combination of multivariate curve resolution-alternating least squares (MCR-ALS) and factorial discriminant analysis (FDA) was proposed. This strategy proved the potential towards the discrimination of healthy and infected leaves by flavescence dorée based on the use of hyperspectral images (Mas Garcia et al., 2021).

Il Soave: esempio di cultura e di scienza

L’evoluzione del settore viti-enologico, supportato dalla ricerca ci propone sempre più frequentemente vini unici e inconfondibili. Il merito di ciò è da ricercare nel rapporto vitigno-territorio e dalla

Red wine substituted esters involved in fruity aromatic expression: an enantiomeric approach to understand their sensory impact and their pathway formation

Among red wines ethyl esters, those from short hydroxylated and branched-chain aliphatic acids constitute a family with a particular behavior and sensory importance. They have been previously discussed in the literature [1] and recent studies have established that some of them were strongly involved in of red wines’ fruity aroma [2]. As some among them have an asymmetrical carbon atom, it seemed important to separate their different enantiomers to obtain an accurate assessment of their organoleptic impact. Three chiral esters have been identified, presenting alkyl and/or hydroxyle substituants: ethyl 2-hydroxy-4-methylpentanoate, ethyl 2-methylbutanoate, and ethyl 3-hydroxybutanoate.