Terroir 2020 banner
IVES 9 IVES Conference Series 9 Soil microbial and arthropod biodiversity under organic and biodynamic viticulture

Soil microbial and arthropod biodiversity under organic and biodynamic viticulture

Abstract

Aims: The aim of the study was to investigate whether organic or biodynamic management have a long-term impact on 1) the microbial biomass and enzymatic activity in the soil, 2) the soil microbial community, 3) flying as well as soil living arthropods and associated fungi.

Methods and Results: The studies presented here were conducted in a field trial comparing integrated, organic and biodynamic viticulture at least 10 years after the implementation of the different management systems. The vineyard is located in Geisenheim, Germany, and the study is conducted on Vitis vinifera L. cv. Riesling.

One study assessed soil enzymatic activities (GLU, CAT, UR, DHA, PHO) and microbial biomass by quantifying PLFAs and NLFAs, respectively. For the second study soil fungal and bacterial biodiversity were investigated using an amplicon sequencing approach. For the third study eDNA was extracted from arthropods in bulk and soil samples. A DNA metabarcoding approach was used to investigate whether diversity of arthropods and fungi in these samples was affected by the management system.

Fungal and bacterial biomass as well as enzymatic activities in the soil were shown to be highly affected by the management system. The organic and the biodynamic systems had significantly more fungal and bacterial biomass. In contrast, the integrated system had a significantly higher mycorrhizal biomass compared to the organic and the biodynamic system. Enzymatic activities measured were significantly higher under organic and biodynamic management.

Fungal species richness assessed by DNA sequencing did not differ among management systems, but fungal community composition was significantly affected. Bacterial species richness was significantly higher under organic and biodynamic management, whereas bacterial community composition was less affected by the management system.

Richness of flying and soil-living arthropods and their related fungi assessed by eDNA sequencing was not significantly affected by the management system alone. In contrast, management systems significantly differed in the arthropod community composition in bulk samples as well as in fungal community composition associated with flying as well as soil-living arthropods.

Conclusions:

Different management systems have a clear impact on soil microbial activity, biomass, and biodiversity, as well as on arthropod biodiversity and fungal biodiversity associated with arthropods. In the current studies soil enzymatic activities as well as soil microbial biomass and bacterial species richness in the soil were positively affected by organic and biodynamic management. Fungal community composition in the soil, in samples of soil-living as well as in samples of flying arthropods were highly affected by the management system. The hypothesis of whether arthropods in the vineyard act as vectors for bacteria and fungi will be discussed.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type: Video

Authors

Johanna Döring1*, Matthias Friedel1, Jacob Agerbo Rasmussen3,4,5, Maximilian Hendgen2, Sofia Di Giacinto2, Randolf Kauer1

1Department of General and Organic Viticulture, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany
2Department of Soil Science and Plant Nutrition, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany
3Section for Evolutionary Genomics, The GLOBE Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Øster Farimagsgade 5A, 1352 Copenhagen, Denmark
4Biological Institute, Genome Research and Molecular Biomedicine, University of Copenhagen, Universitetsparken 13, 2200 Copenhagen, Denmark 
5Center for Evolutionary Hologenomics, University of Copenhagen, Øster Farimagsgade  5A,  1352 Copenhagen, Denmark

Contact the author

Keywords

Organic, biodynamic, soil microbial activity, soil microbial biomass, microbial biodiversity, arthropod biodiversity

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

Downscaling of remote sensing time series: thermal zone classification approach in Gironde region

In viticulture, the challenges of local climate modelling are multiple: taking into account the local environment, fine temporal and spatial scales, reliable time series of climate data, ease of implementation and reproducibility of the method. At the local scale, recent studies have demonstrated the contribution of spatialization methods for ground-based climate observation data considering topographic factors such as altitude, slope, aspect, and geographic coordinates (Le Roux et al, 2017; De Rességuier et al, 2020). However, these studies have shown questions in terms of the reproducibility and sustainability of this type of climate study. In this context, we evaluated the potential of MODIS thermal satellite images validated with ground-based climate data (Morin et al, 2020). Previous studies have been encouraging, but questions remain to be explored at the regional scale, particularly in the dynamics of the massive use of bioclimatic indices to classify the climate of wine regions. The results at the local scale were encouraging, but this approach was tested in the current study at the regional scale. Several objectives were set: 1) to evaluate the downscaling method for land surface temperature time series, 2) to identify regional thermal structure variations. We used weekly minimum and maximum surface temperature time series acquired by MODIS satellites at a spatial resolution of 1000 m and downscaled at 500 m using topographical variables. Two types of analyses were performed: