Terroir 2020 banner
IVES 9 IVES Conference Series 9 Soil microbial and arthropod biodiversity under organic and biodynamic viticulture

Soil microbial and arthropod biodiversity under organic and biodynamic viticulture

Abstract

Aims: The aim of the study was to investigate whether organic or biodynamic management have a long-term impact on 1) the microbial biomass and enzymatic activity in the soil, 2) the soil microbial community, 3) flying as well as soil living arthropods and associated fungi.

Methods and Results: The studies presented here were conducted in a field trial comparing integrated, organic and biodynamic viticulture at least 10 years after the implementation of the different management systems. The vineyard is located in Geisenheim, Germany, and the study is conducted on Vitis vinifera L. cv. Riesling.

One study assessed soil enzymatic activities (GLU, CAT, UR, DHA, PHO) and microbial biomass by quantifying PLFAs and NLFAs, respectively. For the second study soil fungal and bacterial biodiversity were investigated using an amplicon sequencing approach. For the third study eDNA was extracted from arthropods in bulk and soil samples. A DNA metabarcoding approach was used to investigate whether diversity of arthropods and fungi in these samples was affected by the management system.

Fungal and bacterial biomass as well as enzymatic activities in the soil were shown to be highly affected by the management system. The organic and the biodynamic systems had significantly more fungal and bacterial biomass. In contrast, the integrated system had a significantly higher mycorrhizal biomass compared to the organic and the biodynamic system. Enzymatic activities measured were significantly higher under organic and biodynamic management.

Fungal species richness assessed by DNA sequencing did not differ among management systems, but fungal community composition was significantly affected. Bacterial species richness was significantly higher under organic and biodynamic management, whereas bacterial community composition was less affected by the management system.

Richness of flying and soil-living arthropods and their related fungi assessed by eDNA sequencing was not significantly affected by the management system alone. In contrast, management systems significantly differed in the arthropod community composition in bulk samples as well as in fungal community composition associated with flying as well as soil-living arthropods.

Conclusions:

Different management systems have a clear impact on soil microbial activity, biomass, and biodiversity, as well as on arthropod biodiversity and fungal biodiversity associated with arthropods. In the current studies soil enzymatic activities as well as soil microbial biomass and bacterial species richness in the soil were positively affected by organic and biodynamic management. Fungal community composition in the soil, in samples of soil-living as well as in samples of flying arthropods were highly affected by the management system. The hypothesis of whether arthropods in the vineyard act as vectors for bacteria and fungi will be discussed.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type: Video

Authors

Johanna Döring1*, Matthias Friedel1, Jacob Agerbo Rasmussen3,4,5, Maximilian Hendgen2, Sofia Di Giacinto2, Randolf Kauer1

1Department of General and Organic Viticulture, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany
2Department of Soil Science and Plant Nutrition, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany
3Section for Evolutionary Genomics, The GLOBE Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Øster Farimagsgade 5A, 1352 Copenhagen, Denmark
4Biological Institute, Genome Research and Molecular Biomedicine, University of Copenhagen, Universitetsparken 13, 2200 Copenhagen, Denmark 
5Center for Evolutionary Hologenomics, University of Copenhagen, Øster Farimagsgade  5A,  1352 Copenhagen, Denmark

Contact the author

Keywords

Organic, biodynamic, soil microbial activity, soil microbial biomass, microbial biodiversity, arthropod biodiversity

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Elucidating vineyard site contributions to key sensory molecules: Identification of correlations between elemental composition and volatile aroma profile of site-specific Pinot noir wines

The reproducibility of elemental profile in wines produced across multiple vintages has been previously reported using grapes from a single scion clone of Vitis vinifera L. cv. Pinot noir. The grapevines were grown on fourteen different vineyard sites, from Oregon to southern California in the U.S.A., which span distances from approximately hundreds of meters to 1450 km, while elevations range from near sea level to nearly 500 m. In addition, sensorial (i.e. aroma, taste, and mouthfeel) and chemical (i.e. polyphenolic and volatile) differences across the different vineyard sites have also been observed among these wines at two aging time points. While strong evidence exists to support that grapes grown in different regions can produce wines with unique chemical and sensorial profiles, even when a single clone is used, the understanding of growing site characteristics that result in this reproducible differentiation continues to emerge. One hypothesis is that the elemental profile that a vineyard site imparts to the grape berries and the resulting wine is an important contributor to this differentiation in chemistry and sensory of wines. For example, various classes of enzymes that catalyze the formation of key aroma compounds or their precursors require specific metals. In this work, we begin to report correlations between elemental and volatile aroma profiles of site-specific Pinot noir wines, made under standardized winemaking conditions, that have been previously shown to be distinguished separately by these chemical analyses.

Climate modeling at local scale in the Waipara winegrowing region in the climate change context

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.

Current climate change in the Oplenac wine-growing district (Serbia)

Serbian autochthonous vine varieties Smederevka (for white wines) and Prokupac (for rosé and red wines) are the primary representatives of typical characteristics of wines and terroir of numerous wine-growing areas in Serbia. In the past, these varieties were the leading vine varieties, however, as the result of globalization of winemaking and the trend of consumption of wines from widely prevalent vine varieties, they were replaced by introduced international varieties. Smederevka and Prokupac vine varieties are characterized by later time of grape ripening, and relative sensitivity to low temperatures. Climate conditions can be a restrictive factor for production of high-quality grapes and wine and for the spatial spreading of these varieties in hilly continental wine-growing areas.
This paper focuses on the spatial analysis of changes of main climate parameters, in particular, analysis of viticultural bioclimatic indices that were determined for the purposes of viticulture zoning of wine-growing areas in the period 1961-2010, and those same parameters determined for the current, that is, referential climate period (1988-2017). Results of the research, that is, analysis of climate changes indicate that the majority of examined climate parameters in the Oplenac wine-growing district improved from the perspective of Smederevka and Prokupac vine varieties. These studies of climate conditions indicate that changes of analyzed climate parameters, that is, bioclimatic indices will be favorable for cultivation of varieties with later grape ripening times and those more sensitive to low temperatures, such as the autochthonous vine varieties Smederevka and Prokupac, therefore, it is recommended to producers to more actively plant vineyards with these varieties in the territory of the Oplenac wine-growing district.

Local ancient grapevine cultivars to face future viticulture

Among the different strategies to cope with the negative impacts of climate change on viticulture, the exploitation of genetic diversity is one of the most promising to adapt to new conditions and maintain wine production and quality. One of the biggest concerns in the context of climate change is to improve water use efficiency (WUE). In this way, the use of genotypes that present a better response to drought and high WUE is a key issue. In this work, physiological performance analysis was conducted to compare the water deficit stress (WDS) responses of local and widespread grapevines cultivars. Leaf gas exchange, water use efficiency (WUE) at different levels (leaf and long-term WUE (∆13C)), leaf osmotic adjustment and other water relations parameters were determined in plants under well-watered and WDS conditions alongside assessment of the levels of foliar hormones concentrations. Results denote that local cultivars displayed better physiological performance under WDS as compared to the widely-distributed ones. he results corroborate the hypothesis that better stomatal control allows increasing leaf WUE under drought as occurred in the local Callet cv.; but the minority local cultivar Escursac cv. showed high WUE under both treatments. In this case, high WUE can be related to maintaining higher photosynthetic activity under drought. The different mechanisms underlying the better performance under WDS and high WUE of minority local cultivars are discussed.