Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Measurement of trans-membrane and trans-tissue voltages in the Shiraz berry mesocarp

Measurement of trans-membrane and trans-tissue voltages in the Shiraz berry mesocarp

Abstract

AIM: In mid to late ripening, sugar and potassium (K+) accumulation into the berry slows and is eventually completed1. K+ is the most abundant cation in the berry, undertaking important physiological roles. During late ripening, Shiraz mesocarp cells die within the central region of the berry. The cessation of K+ import may be a contributing factor to this loss in cell vitality. Many K+ trans-membrane transporters and channels are regulated by the membrane voltage (Vm). We thus measured trans-membrane voltage (Vm) and trans-tissue voltages (Vt) in the mesocarp during Shiraz berry development.

METHODS: Vm measurement Shiraz berries, grown in Coombe vineyard at the University of Adelaide, were sampled weekly from the completion of véraison to the late-ripening stage. To assess Vm, the microelectrode was inserted through the berry skin and into mesocarp. During injection, voltage signals and the corresponding depths of the micropipette tip were recorded. Vt measurement The Vt was measured by a similar method described above without micropipette injection. A small piece of skin was removed, allowing the measurement of Vt from the pedicel to the mesocarp surface. Living berries and dead berries from véraison and late-ripening stage were used. Dead berries were measured after freezing overnight followed by thawing.

RESULTS: Vm The voltages became less negative with increasing tissue depth. This may be attributed to the more severe hypoxia within deeper regions of the berry2. Voltage responses were detected in both living berries and dead berries in the late-ripening stage, with similar profiles. This indicates that other structures or factors contributed to the voltage detected by this method. Vt In living berries, the Vt values were more negative in véraison berries than those in late-ripening berries. This trend was not observed in dead berries. There was no significant difference between the Vt values measured from living berries and dead berries in late-ripening stage.

CONCLUSIONS

The uneven distribution of the Vm between berry compartments may be correlated with oxygen concentration, which could impact on K+ transport within berries. The declined Vm and Vt in the late ripening berries could be associated with the cessation of K+ import into berries.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Yin Liu 

National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW 2678, Australia,Suzy ROGIERS (New South Wales Department of Primary Industries, Wagga Wagga, NSW 2678, Australia) Leigh SCHMIDTKE (National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW 2678, Australia) Stephen TYERMAN (School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia)

Contact the author

Keywords

grape berry ripening, microelectrode, voltage, mesocarp

Citation

Related articles…

Soil, vine, climate change – what is observed – what is expected

To evaluate the current and future impact of climate change on Viticulture requires an integrated view on a complex interacting system within the soil-plant-atmospheric continuum under continuous change. Aside of the globally observed increase in temperature in basically all viticulture regions for at least four decades, we observe several clear trends at the regional level in the ratio of precipitation to potential evapotranspiration. Additionally the recently published 6th assessment report of the IPCC (The physical science basis) shows case-dependent further expected shifts in climate patterns which will have substantial impacts on the way we will conduct viticulture in the decades to come.
Looking beyond climate developments, we observe rising temperatures in the upper soil layers which will have an impact on the distribution of microbial populations, the decay rate of organic matter or the storage capacity for carbon, thus affecting the emission of greenhouse gases (GHGs) and the viscosity of water in the soil-plant pathway, altering the transport of water. If the upper soil layers dry out faster due to less rainfall and/or increased evapotranspiration driven by higher temperatures, the spectral reflection properties of bare soil change and the transport of latent heat into the fruiting zone is increased putting a higher temperature load on the fruit. Interactions between micro-organisms in the rhizosphere and the grapevine root system are poorly understood but respond to environmental factors (such as increased soil temperatures) and the plant material (rootstock for instance), respectively the cultivation system (for example bio-organic versus conventional). This adds to an extremely complex system to manage in terms of increased resilience, adaptation to and even mitigation of climate change. Nevertheless, taken as a whole, effects on the individual expressions of wines with a given origin, seem highly likely to become more apparent.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Effect of one-year cover crop and arbuscular mycorrhiza inocululation in the microbial soil community of a vineyard

The microbial composition of the soil is an important factor to consider in viticulture, since its influence on the “terroir” and on the organoleptic properties of the wine have been demonstrated. Different agronomic techniques have the potential to modify the composition and functionality of the soil microbial community. Maintaining green covers is known to increase soil microbial diversity. The direct application of inoculum of beneficial microorganisms to the soil has also been used to increase their abundance. However, the environmental conditions of each site seem to have a determining weight in the result of these practices. In this study, we compared the effect on the microbial community of a cover crop with legumes in autumn and the inoculation of grapevines with commercial inoculum bases on Rhizophagus irregularis and Funeliformis mosseae in the previous spring. The study has been carried out in a vineyard in Binissalem, Mallorca, Spain. After applying the treatments, we will analyze the soil microbial communities using the data obtained from Illumina amplification of soil DNA from the 16S and ITS regions to analyze bacteria and fungi community, respectively. In addition, we will record the physicochemical characteristics of the soil at each sampling point. The result showed that agronomic management, in the short term, has less influence than soil characteristics on the composition of the soil microbiome. With these results, we can conclude that in a vineyard, agricultural techniques should focus on improving the characteristics of the soil to improve the biodiversity of the soil microbiota.

Spatial variability of temperature is linked to grape composition variability in the Saint-Emilion winegrowing area

Elevated temperature during the grape maturation period is a major threat for grape quality and thus wine quality. Therefore, characterizing the grape composition response to temperature at a larger scale would represent a crucial step towards adaptation to climate change. In response to changes in temperature, various physiological mechanisms regulate grape composition. Primary and secondary metabolisms are both involved in this response, with well-known effects, for example on anthocyanins, and lesser known effects, for example on aromas or aroma precursors. At the field scale or at the regional scale, however, numerous environmental or plant-specific factors intervene to make the effects of temperature difficult to distinguish from overall variability. In this study, it was attempted to overcome this difficulty by selecting well-characterized situations with differing temperatures.
A long-term study of air temperature variability across several Merlot vineyards in the Saint-Emilion and Pomerol wine producing area found significant temperature differences and gradients at various time scales linked to environmental factors. From this study area, a few sites were selected with similar age, soil and training system conditions, and with repeated and contrasted temperature differences during the maturation period. The average temperature difference during the maturation period was about 2°C between cooler and warmer sites, a difference similar to that expected under future climate change scenarios. In close vicinity to the temperature sensors at each site, grape berries were sampled at different times until full maturity during 2019 and 2020. Also, berries from bunches on either side of the row were analyzed separately, allowing an investigation of bunch exposure effect associated with the coupling of berry temperature and solar radiation. Four replicates of pooled berries for each time – site – bunch exposure combination were obtained and analyzed for biochemical composition. Analyses of variance of the biochemical composition data collected at different sampling times reveal significant effects associated with temperature, site, and bunch azimuth. For instance, anthocyanins in grape skins are clearly influenced by temperature and solar radiation exposure, with up to 30% reduction in warmer conditions.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)