Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Targeted UHPLC-QqQ-MS/MS metabolomics for phenol identification in grapevine and wine: study of a Tempranillo clone with a dark-blue berry colour

Targeted UHPLC-QqQ-MS/MS metabolomics for phenol identification in grapevine and wine: study of a Tempranillo clone with a dark-blue berry colour

Abstract

Grapevine vegetative multiplication allows the accumulation of spontaneous mutations and increase intra-cultivar genetic diversity that can be exploited to maintain grape wine quality, tipicity and adaptation to different climate conditions. Non-volatile phenolic compounds are intrinsic components of grape fruits and derived products, particularly wine. They constitute a heterogeneous family of compounds and play an important role on the sensorial attributes of wine because they are responsible for some of important organoleptic properties as colour, flavour, bitterness and astringency. In the present study, we used a targeted metabolomics approach based on ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC-QqQ-MS/MS) to study the anthocyanin and non-coloured phenol profiles of a singular Tempranillo clone (Tempranillo negro or VN21), characterized by a dark-blue color in grape berry skin, as compared to RJ43, one of the most cultivated clones in D.O.Ca. Rioja (Spain). In addition, we investigated differences between VN21 and RJ43 clones, in the phenolic transference from grape to wine at different phases of the winemaking process. The results showed that anthocyanin and non-colored phenol content was higher in VN21 grape skin and seeds than in RJ43. With respect to anthocyanins, the singular color of grape skin in VN21 could be explained by higher concentrations of peonidin and cyanidin derivatives. Regarding non-colored phenols, the main differences were observed for proanthocyanidins and stilbenes concentration in grape skin and more importantly in seeds. Those content differences observed in berries were enhanced in the VN21 wines, displaying significantly higher concentrations of anthocyanins, as well as significantly increased contents of mainly proanthocyanidins and stilbenes. The results manifest the importance of intra-cultivar genetic diversity to obtain red wines with a high phenolic content, responsible of key quality aspects of the wine such as organoleptic properties, stability, complexity and health benefits. Moreover, this study exemplifies how spontaneous somatic variation can be used through grapevine clonal selection combining metabolomic analyses.

FUNDING SOURCES

This work was partially supported by project BIO2017-86375-R from the Spanish Ministry of Economy and Competitiveness (co-funded by the European Social Fund, European Union); YF was supported by a grant from Government of La Rioja; M.J. Motilva thanks to CSIC for partial funding through the “Ayudas incorporación a escalas científicas CSIC, 2018” (Reference 201870I129).

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Yolanda Ferradás 

Instituto de Ciencias de la Vid y del Vino (Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja), Finca La Grajera, Ctra. de Burgos Km. 6 (LO-20 – salida 13). 26007 Logroño (La Rioja), Spain,Carolina ROYO, José Miguel MARTÍNEZ-ZAPATER and María José MOTILVA  Instituto de Ciencias de la Vid y del Vino (Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja), Finca La Grajera, Ctra. de Burgos Km. 6 (LO-20 – salida 13). 26007 Logroño (La Rioja), Spain

Contact the author

Keywords

anthocyanins, berry phenolic composition, wine phenolic composition, somatic variation, grapevine, phenolic compounds, stilbenes, tempranillo

Citation

Related articles…

1H-NMR-based Untargeted Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

Untargeted metabolomics has proven to be an effective method to study the impact of the terroir on metabolic profile of wines. In this context, the aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through 1H-NMR metabolomics combined with chemometrics.Grapes from Nero d’Avola L. red cultivar cultivated on four different soil types were separately vinified to obtain four different red wines.One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz

Effect of quercus alba oak barrels from different forest on the volatile composition of Tempranillo wines

The species and origin used for red wine oak aging determines the physiological composition of the wood and thus the finished wines. In America, oak is grown primarily

Impact of copper residues in grape must on alcoholic fermentation: effects on yeast performance, acetaldehyde and SO2 production

A relevant trend in winemaking is to reduce the use of chemical compounds in both the vineyard and winery.

Green berries on Gewürztraminer (Vitis vinifera L.) in South Tyrol (Italy)

The grape variety Gewürztraminer is known to be affected by two physiological disorders namely berry shrivel and bunch stem necrosis. During the season 2014 we noticed a new symptomatology type of ripening disorder on the variety. The new symptom showed not all berries fallowing the normal maturation stages, but single berries remaining at a soft but green stage till harvest. The broad distribution of these so called “green berries” symptoms in different production sites of our region, caused huge damage due to the difficulty of eliminating single berries per bunch before harvesting. Therefore, the Research Centre Laimburg began to investigate the reasons and origins of this new symptom. This work shows the results of first attempts to find causes for the symptom as well as the resulting approach to mitigate symptoms. Applications of magnesium leaf fertilizer showed first promising results against this putative disorder. To study the causal effect of the green berries 30 symptomatic vineyards in 2014 have been selected for a monitoring during the season 2016. To evaluate the foliar nutrient treatment two vineyards have been selected for application of magnesium sulfate and magnesium chloride. Leaf and berry nutrient analysis, as well as the main quality parameters during ripening have been performed. As soon as “green berries” symptoms appeared, incidence and severity have been evaluated. Most of the symptomatic vineyards of the 2016 monitoring showed light to clear magnesium deficit symptoms on their foliage. Only during the seasons 2020 and 2021 “green berries” symptoms could be found in the leaf fertilizer treatment vineyards. Both seasons showed a significant effect of the magnesium treatments to reduce the incidence and severity of the symptom. It seems that the appearance of the “green berries” symptom on Gewürztraminer is correlated to a disturbed uptake of magnesium of the vines.

Budburst delay and berry ripening after vegetal oil application in Austria

Occurrence of freezing temperatures in early spring when grapevine shoots are developing is termed late frost in viticulture. Young green tissues are very sensible to temperatures below zero and damages often lead to important yield and quality losses such as the case in Europe in 2017. An indirect method to avoid late frost damage in vineyards consist in delaying the budburst. Previous research reported similar effects by applying vegetal oil on dormant buds. Here, we tested the application of rapeseed vegetal oil during late winter to delay the budburst on two V.vinifera cultivars of interest in Austria, Grüner Veltliner (GV) and Zweigelt (ZW).