Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Carbon isotope labeling to detect source-sink relationships in grapevines upon drought stress and re-watering

Carbon isotope labeling to detect source-sink relationships in grapevines upon drought stress and re-watering

Abstract

AIM: Kinetics of carbon allocation in the different plant sinks (root-shoot-fruit) competing in drought stressed and rehydrated grapevines have been investigated.

METHODS: A plant growth chamber for stable isotope labeling has been set in an environmental control system, basing on pulse-chasing isotopic strategy to trace carbon phloem flows on potted grapevines.In addition, an open-air plant/soil growth system consisting in twelve independent plant/pot balloons with computing-adjustable air flows allowing continuous gas exchange detection between plants / soil and atmosphere has been set.

RESULTS: Water stress caused a drastic decrease in the photosynthesis rate and a decrease in the respiration rate of the soil by about 50%; after rehydration the plants fully recovered the photosynthetic capacity in the morning, while the photosynthetic capacity in the afternoon remained compromised. Sugar accumulation in berries decreased in plants subjected to continuous stress, while the acidity was higher for both plants subjected to continuous stress and rehydrated plants. Grape production was lower in plants subjected to continuous stress.Plants under water stress had a low and constant microbial biomass throughout the season, whereas irrigated and rehydrated plants remained similar in the first days of the experiment, and an explosion of microbial biomass was recorded in plants rehydrated 15 days after rehydration. This may indicate a higher contribution of carbon allocated by the rehydrated plant to the microbial mass of the rhizosphere.

CONCLUSIONS

Water stress causes a greater diversion of the newly photosynthesized carbonaceous resources to the berry (about double compared to irrigation controls). The carbon accumulated in the berry is stored in a stable manner. The carbon diverted to the root over 30 days is mostly consumed.The plant in recovery diverts the same percentage of carbon marked to the berry of the plants in water stress although in absolute its photosynthesis is about double than under water stress (it is comparable or even higher than photosynthesis un irrigated control plants); therefore the total C sent to the berry is greater in recovery than in irrigation control.Through a daily respired / photosynthesized C balance we show that during the ripening of the berry 60% of the C assimilated in the irrigated condition is respired. Since the accumulation of neo-photosynthetate is stable at 27%, this amount does not affect the reserves accumulated in the pre-veraison root.Delivery of labeled carbon in different sinks is discussed in parallel with the expression of genes involved in carbohydrate transport. Financial support: CARBOSTRESS project – CRT – Cassa Risparmio Torino Foundation.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Davide Lucien Patono

Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy,Daniel, SAID PULLICINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Leandro, ELOI ALCATRAO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Giorgio, IVALDI, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Andrea, FIRBUS, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Giorgio, GAMBINO, Institute for Sustainable Plant Protection, National Research Council, Turin, Italy  Irene, PERRONE, Institute for Sustainable Plant Protection, National Research Council, Turin, Italy  Walter, CHITARRRA, Centro di Ricerca Viticoltura ed Enologia VE, CREA, Conegliano, Italy  Alessandra, FERRANDINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Davide, RICAUDA AIMONINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Luisella, CELI, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Claudio, LOVISOLO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy

Contact the author

Keywords

drought, carbon isotope labeling, respiration, photosynthesis, phloem

Citation

Related articles…

Effect of partial net shading on the temperature and radiation in the grapevine canopy, consequences on the grape quality of cv. Gros Manseng in PDO Pacherenc-du-vic-Bilh

As elsewhere, southwestern France vineyards face more recurrent summer heat waves these last years. Among the possibilities of adaptation to this climate changing parameter, the use of net shading is a technique that allow for limiting canopy exposure to radiations. In this trial, we tested net shading installed on one face of the canopy, on a north-south row-oriented plot of cv. Gros Manseng trained on VSP system in the PDO Pacherenc-du-Vic-Bilh. The purpose was to characterize the effects on the ambient canopy temperatures and radiations during the season and to observe the consequences on the composition of grapes and wines. Two sorts of net were used with two levels of obstruction (50% and 75%) of the photosynthesis active radiation (PAR). They have been installed on the west side of the canopy and compared to a netless control. Temperature and PAR sensors registered hourly data during the season. On specific summer day (hot and sunny) manual measurements took also place on bunches (temperature) and in different spots of the canopy (PAR). The results showed that, on clear days, the radiation is lowered by the shade nets respecting the supplier criteria. The effects on the ambient canopy temperature were inconstant on this plot when we observed the data from the global period of shading between fruit set and harvest. However, during hot days (>30°C), the temperature in the canopy was reduced during afternoon and the temperature of the bunch surface was reduced as well comparing to the control. A decrease of the maturity parameters of the berries, sugar and acidity, was also observed. Concerning the wine aromatic potential, no differences clearly appeared.

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.

A better understanding of the climate effect on anthocyanin accumulation in grapes using a machine learning approach

The current climate changes are directly threatening the balance of the vineyard at harvest time. The maturation period of the grapes is shifted to the middle of the summer, at a time when radiation and air temperature are at their maximum. In this context, the implementation of corrective practices becomes problematic. Unfortunately, our knowledge of the climate effect on the quality of different grape varieties remains very incomplete to guide these choices. During the Innovine project, original experiments were carried out on Syrah to study the combined effects of normal or high air temperature and varying degrees of exposure of the berries to the sun. Berries subjected to these different conditions were sampled and analyzed throughout the maturation period. Several quality characteristics were determined, including anthocyanin content. The objective of the experiments was to investigate which climatic determinants were most important for anthocyanin accumulation in the berries. Temperature and irradiance data, observed over time with a very thin discretization step, are called functional data in statistics. We developed the procedure SpiceFP (Sparse and Structured Procedure to Identify Combined Effects of Functional Predictors) to explain the variations of a scalar response variable (a grape berry quality variable for example) by two or three functional predictors (as temperature and irradiance) in a context of joint influence of these predictors. Particular attention was paid to the interpretability of the results. Analysis of the data using SpiceFP identified a negative impact of morning combinations of low irradiance (lower than about 100 μmol m−2 s−1 or 45 μmol m−2 s−1 depending on the advanced-delayed state of the berries) and high temperature (higher than 25oC). A slight difference associated with overnight temperature occurred between these effects identified in the morning.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.

Local ancient grapevine cultivars to face future viticulture

Among the different strategies to cope with the negative impacts of climate change on viticulture, the exploitation of genetic diversity is one of the most promising to adapt to new conditions and maintain wine production and quality. One of the biggest concerns in the context of climate change is to improve water use efficiency (WUE). In this way, the use of genotypes that present a better response to drought and high WUE is a key issue. In this work, physiological performance analysis was conducted to compare the water deficit stress (WDS) responses of local and widespread grapevines cultivars. Leaf gas exchange, water use efficiency (WUE) at different levels (leaf and long-term WUE (∆13C)), leaf osmotic adjustment and other water relations parameters were determined in plants under well-watered and WDS conditions alongside assessment of the levels of foliar hormones concentrations. Results denote that local cultivars displayed better physiological performance under WDS as compared to the widely-distributed ones. he results corroborate the hypothesis that better stomatal control allows increasing leaf WUE under drought as occurred in the local Callet cv.; but the minority local cultivar Escursac cv. showed high WUE under both treatments. In this case, high WUE can be related to maintaining higher photosynthetic activity under drought. The different mechanisms underlying the better performance under WDS and high WUE of minority local cultivars are discussed.