Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Carbon isotope labeling to detect source-sink relationships in grapevines upon drought stress and re-watering

Carbon isotope labeling to detect source-sink relationships in grapevines upon drought stress and re-watering

Abstract

AIM: Kinetics of carbon allocation in the different plant sinks (root-shoot-fruit) competing in drought stressed and rehydrated grapevines have been investigated.

METHODS: A plant growth chamber for stable isotope labeling has been set in an environmental control system, basing on pulse-chasing isotopic strategy to trace carbon phloem flows on potted grapevines.In addition, an open-air plant/soil growth system consisting in twelve independent plant/pot balloons with computing-adjustable air flows allowing continuous gas exchange detection between plants / soil and atmosphere has been set.

RESULTS: Water stress caused a drastic decrease in the photosynthesis rate and a decrease in the respiration rate of the soil by about 50%; after rehydration the plants fully recovered the photosynthetic capacity in the morning, while the photosynthetic capacity in the afternoon remained compromised. Sugar accumulation in berries decreased in plants subjected to continuous stress, while the acidity was higher for both plants subjected to continuous stress and rehydrated plants. Grape production was lower in plants subjected to continuous stress.Plants under water stress had a low and constant microbial biomass throughout the season, whereas irrigated and rehydrated plants remained similar in the first days of the experiment, and an explosion of microbial biomass was recorded in plants rehydrated 15 days after rehydration. This may indicate a higher contribution of carbon allocated by the rehydrated plant to the microbial mass of the rhizosphere.

CONCLUSIONS

Water stress causes a greater diversion of the newly photosynthesized carbonaceous resources to the berry (about double compared to irrigation controls). The carbon accumulated in the berry is stored in a stable manner. The carbon diverted to the root over 30 days is mostly consumed.The plant in recovery diverts the same percentage of carbon marked to the berry of the plants in water stress although in absolute its photosynthesis is about double than under water stress (it is comparable or even higher than photosynthesis un irrigated control plants); therefore the total C sent to the berry is greater in recovery than in irrigation control.Through a daily respired / photosynthesized C balance we show that during the ripening of the berry 60% of the C assimilated in the irrigated condition is respired. Since the accumulation of neo-photosynthetate is stable at 27%, this amount does not affect the reserves accumulated in the pre-veraison root.Delivery of labeled carbon in different sinks is discussed in parallel with the expression of genes involved in carbohydrate transport. Financial support: CARBOSTRESS project – CRT – Cassa Risparmio Torino Foundation.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Davide Lucien Patono

Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy,Daniel, SAID PULLICINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Leandro, ELOI ALCATRAO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Giorgio, IVALDI, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Andrea, FIRBUS, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Giorgio, GAMBINO, Institute for Sustainable Plant Protection, National Research Council, Turin, Italy  Irene, PERRONE, Institute for Sustainable Plant Protection, National Research Council, Turin, Italy  Walter, CHITARRRA, Centro di Ricerca Viticoltura ed Enologia VE, CREA, Conegliano, Italy  Alessandra, FERRANDINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Davide, RICAUDA AIMONINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Luisella, CELI, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Claudio, LOVISOLO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy

Contact the author

Keywords

drought, carbon isotope labeling, respiration, photosynthesis, phloem

Citation

Related articles…

Amino nitrogen content in grapes: the impact of crop limitation

As an essential element for grapevine development and yield, nitrogen is also involved in the winemaking process and largely affects wine composition. Grape must amino nitrogen deficiency affects the alcoholic fermentation kinetics and alters the development of wine aroma precursors. It is therefore essential to control and optimize nitrogen use efficiency by the plant to guarantee suitable grape nitrogen composition at harvest. Understanding the impact of environmental conditions and cultural practices on the plant nitrogen metabolism would allow us to better orientate our technical choices with the objective of quality and sustainability (less inputs, higher efficiency). This trial focuses on the impact of crop limitation – that is a common practice in European viticulture – on nitrogen distribution in the plant and particularly on grape nitrogen composition. A wide gradient of crop load was set up in a homogeneous plot of Chasselas (Vitis vinifera) in the experimental vineyard of Agroscope, Switzerland. Dry weight and nitrogen dynamics were monitored in the roots, trunk, canopy and grapes, during two consecutive years, using a 15N-labeling method. Grape amino nitrogen content was assessed in both years, at veraison and at harvest. The close relationship between fruits and roots in the maintenance of plant nitrogen balance was highlighted. Interestingly, grape nitrogen concentration remained unchanged regardless of crop load to the detriment of the growth and nitrogen content of the roots. Meanwhile, the size and the nitrogen concentration of the canopy were not affected. Leaf gas exchange rates were reduced in response to lower yield conditions, reducing carbon and nitrogen assimilation and increasing intrinsic water use efficiency. The must amino nitrogen profiles could be discriminated as a function of crop load. These findings demonstrate the impact of plant balance on grape nitrogen composition and contribute to the improvement of predictive models and sustainable cultural practices in perennial crops.

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.

Impact of changes in pruning practices on vine growth and yield

A gradual decline in vineyards has been observed over the past twenty years worldwide. This might be explained by the climate change, practices change or the increase of dieback diseases. To increase the longevity of vines, we studied the impact of different pruning strategies in four adult and four young vineyards located in France and Spain. In France, vineyards were planted with Cabernet franc on 3309C while Spanish trials were planted with Tempranillo grafted on 110R. Vegetative expression, yield, quality of berries and wood vessels conductivity were measured. The distribution of vegetative expression, yield and berry composition between primary and secondary vegetation were quantified. Finally, tomography was used to evaluate the implication of the treatments on sap flows.
First results show that i) the respectful pruning leads to an increase of 30 to 50% more secondary shoots than the aggressive pruning in France and between 15 and 20% in Spain, ii) there is no major effect on the yield over the first two years following the implementation of the new pruning practices, although the proportion of clusters from suckers is higher on the respectful pruning method. On young vines, the development of the trunk according to a respectful pruning leads to a loss of harvest 2 years after planting. This is due to the removal, on the future trunk, of the green suckers which carrying bunches. This operation carried out in spring rather than during winter pruning, would promote a better leaf / fruit balance when the plant comes into production, and could lead to better hydraulic conduction in the vessels of the trunk. Maintaining these trials for several years will provide more robust data to assess the impact of these practices on the vines over the long term.

Climate change projections to support the transition to climate-smart viticulture

The Earth’s system is undergoing major changes through a wide range of spatial and temporal scales as a response to growing anthropogenic radiative forcing, which is pushing the whole system far beyond its natural variability. Sources of greenhouse gases largely exceed their sinks, thus leading to a strengthened greenhouse effect. More energy is thereby being supplied to the system, with inevitable shifts in climatic patterns and weather regimes. Over the last decades, these modifications have been manifested in the full statistical distributions of the atmospheric variables, with dramatic changes in the frequency and intensity of extremes. Natural hazards, such as severe droughts, floods, forest fires, or heatwaves, are being triggered by extreme atmospheric events worldwide, thus threatening human activities. Viticultculture is not only exposed to changing climates but is also highly vulnerable, as grapevine phenology and physiological development are strongly controlled by atmospheric conditions. Therefore, the assessment of climate change projections for a given region is critical for climate change adaptation and risk reduction in viticulture. By adopting timely and suitable measures, the future sustainability and resiliency of the sector can be fostered. Climate-grapevine chain modelling is an essential tool for better planning and management. However, the accuracy of the resulting projections is limited by many uncertainties that must be duly taken into account when transferring knowledge to stakeholders and decision-makers. Climate-smart viticulture will comprise ensembles of locally tuned strategies, envisioning both adaptation and mitigation, assisted by emerging technologies and decision-support systems.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.