Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Carbon isotope labeling to detect source-sink relationships in grapevines upon drought stress and re-watering

Carbon isotope labeling to detect source-sink relationships in grapevines upon drought stress and re-watering

Abstract

AIM: Kinetics of carbon allocation in the different plant sinks (root-shoot-fruit) competing in drought stressed and rehydrated grapevines have been investigated.

METHODS: A plant growth chamber for stable isotope labeling has been set in an environmental control system, basing on pulse-chasing isotopic strategy to trace carbon phloem flows on potted grapevines.In addition, an open-air plant/soil growth system consisting in twelve independent plant/pot balloons with computing-adjustable air flows allowing continuous gas exchange detection between plants / soil and atmosphere has been set.

RESULTS: Water stress caused a drastic decrease in the photosynthesis rate and a decrease in the respiration rate of the soil by about 50%; after rehydration the plants fully recovered the photosynthetic capacity in the morning, while the photosynthetic capacity in the afternoon remained compromised. Sugar accumulation in berries decreased in plants subjected to continuous stress, while the acidity was higher for both plants subjected to continuous stress and rehydrated plants. Grape production was lower in plants subjected to continuous stress.Plants under water stress had a low and constant microbial biomass throughout the season, whereas irrigated and rehydrated plants remained similar in the first days of the experiment, and an explosion of microbial biomass was recorded in plants rehydrated 15 days after rehydration. This may indicate a higher contribution of carbon allocated by the rehydrated plant to the microbial mass of the rhizosphere.

CONCLUSIONS

Water stress causes a greater diversion of the newly photosynthesized carbonaceous resources to the berry (about double compared to irrigation controls). The carbon accumulated in the berry is stored in a stable manner. The carbon diverted to the root over 30 days is mostly consumed.The plant in recovery diverts the same percentage of carbon marked to the berry of the plants in water stress although in absolute its photosynthesis is about double than under water stress (it is comparable or even higher than photosynthesis un irrigated control plants); therefore the total C sent to the berry is greater in recovery than in irrigation control.Through a daily respired / photosynthesized C balance we show that during the ripening of the berry 60% of the C assimilated in the irrigated condition is respired. Since the accumulation of neo-photosynthetate is stable at 27%, this amount does not affect the reserves accumulated in the pre-veraison root.Delivery of labeled carbon in different sinks is discussed in parallel with the expression of genes involved in carbohydrate transport. Financial support: CARBOSTRESS project – CRT – Cassa Risparmio Torino Foundation.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Davide Lucien Patono

Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy,Daniel, SAID PULLICINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Leandro, ELOI ALCATRAO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Giorgio, IVALDI, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Andrea, FIRBUS, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Giorgio, GAMBINO, Institute for Sustainable Plant Protection, National Research Council, Turin, Italy  Irene, PERRONE, Institute for Sustainable Plant Protection, National Research Council, Turin, Italy  Walter, CHITARRRA, Centro di Ricerca Viticoltura ed Enologia VE, CREA, Conegliano, Italy  Alessandra, FERRANDINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Davide, RICAUDA AIMONINO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Luisella, CELI, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy  Claudio, LOVISOLO, Dept. Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy

Contact the author

Keywords

drought, carbon isotope labeling, respiration, photosynthesis, phloem

Citation

Related articles…

Investigating the impact of bottle color, temperature and light exposure on rose wine characteristics

Rosé is leading the fastest growth wine category which hit a 40% increase since 2002. France accounts for over a third (34%) of global consumption followed by the US

Population-wide diversity study in Lachancea thermotolerans highlights superior starters for winemaking

Grapes from warm(ing) climates often contain excessive sugars but lack acidity. This can lead to highly alcoholic wines with compromised stability and balance. The yeast Lachancea thermotolerans can ameliorate such wines due to its metabolic peculiarity – partial fermentation of sugars to lactic acid. This study aimed to elucidate the population-wide diversity in L. thermotolerans, whilst selecting superior strains for wine sector. An extensive collection of isolates (~200) sourced from different habitats worldwide was first genotyped on 14 microsatellite loci. This revealed differentiation of L. thermotolerans genetic groups based on the isolation substrate and geography. The 94 genotyped strains were then characterised in Vitis vinifera cv. Chardonnay fermentations.

High resolution remote sensing for mapping intra-block vine vigour heterogeneity

In vineyard management, the block is considered today as the technical work unit. However, considerable variability can exist inside a block with regard to physiological parameters, such as vigour, particularly because of soil heterogeneity. To represent this variability spatially, many measurements have to be taken, which is costly in both time and money. High resolution remote sensing appears to be an efficient tool for mapping intra-block heterogeneity.

Production and technological characteristics of some French clones of the Chardonnay variety in Yugoslavia

L’observation est effectuée entre 1996 et 1998. L’expérience a commencé avec des clones numérotés: 75, 95, 96 et 227 de la variété Chardonnay. Le porte greffe est le Kober 5 BB. La forme de conduite est le cordon. La taille est longue. La densité de plantation est 3,5 x 1 mètre (2857 ceps par 1/ha).

Résistance stomatique et caractérisation hydrique des terroirs viticoles

The analysis of the distribution of natural plant populations allows an ecological characterization of cultivated environments in thermal, water and trophic terms; it guides the choice or selection of plants (or grape varieties) to cultivate (Astruc et al ., 1984, 1987; Delpoux, 1971; Jacquinet and Astruc, 1979). This approach has given good results in areas where the topography is the determining factor in the ecological differentiation of the terroirs.