Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 First characterization of thiol precursors in colombard and gros manseng: comparison of two cultivation practices

First characterization of thiol precursors in colombard and gros manseng: comparison of two cultivation practices

Abstract

AIM: Organic production of wine in the past years has known an important augmentation. This type of cultivation practice switches synthetic phytosanitary product for copper-based protection as fungicide. While the effect of copper on soil and vine is understood, few data are published concerning its impact on wine aromas (Darriet et al. 2001) and even less concerning varietal thiol. The aim of this work was to characterize the thiol aromatic potential of Colombard and Gros Manseng grapes and to investigate the effect of copper on thiol precursor biogenesis. We selected 30 parcels (15 conventional and 15 organic) with sampling at harvest for 18 of them and 3 sampling dates during ripening for the other 12 parcels. Chemical analyses of thiol precursors were performed by adapting an UPLC-MS/MS method based upon Stable Isotope Dilution Assay (Bonnaffoux et al. 2017)

RESULTS: With this first characterization, we demonstrated that both varieties presented concentrations of glutathionylated (G3SH) and cysteinylated (Cys3SH) precursors of 3-sulfanylhexan-1-ol up to 454 µg/kg and 21 µg/kg respectively. No precursors of the 4-sulfanyl-4-methylpentan-2-one were detected. So, Colombard and Gros Manseng were ranked in the top of varietal thiol producers with Sauvignon and Pinot Gris grapes (Pena-Gallego et al. 2012). By comparing the copper protected parcels to the others, we identified a significant decrease (p-value = 0.01) of G3SH content in organic Gros Manseng grapes which was around 30%. This could represent a loss in 3SH of 1000 ng/kg in finished wines if a conversion rate of 3% between G3SH and 3SH was considered. On the opposite, Colombard grapes remained unaffected by the copper spraying with no effect on the precursors content at harvest. We also analyzed the accumulation kinetics of thiol precursors under the two protection methods (copper or not). No accumulation of thiol precursors between seven days prior and after the harvest was observed in Colombard grapes which was inconsistent with literature on other grape varieties such as Sauvignon B. or Melon B. (Roland et al. 2010). Gros Manseng grapes showed significant accumulation for conventional culture (161 µg/kg to 356 µg/kg) over the two last weeks of ripening. Furthermore, copper treated parcels of Gros Manseng have no accumulation through the two-week study. However, Pearson test did not show a direct correlation between copper content and G3SH suggesting a possible interaction of copper with thiol precursors making them not analyzable under our conditions or a modification of vine metabolism.

CONCLUSION:

We characterized for the first time two grapes varieties (Colombard and Gros Manseng) that have high potential towards varietal thiols such as Sauvignon. We identified a negative copper effect on Gros Manseng variety for both precursors content at harvest and accumulation kinetics while Colombard remained unaffected.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Gabriel Dournes, Arnaud VERBAERE, Frédéric LOPEZ, Thierry DUFOURCQ, Jean-Roch MOURET, Aurélie ROLAND

SPO, Univ Montpellier, INRAE, Institut Agro, Montpellier, France, IFV Sud-Ouest, Château de Mons, 32100 Caussens, France

Contact the author

Keywords

copper, thiol precursors, colombard, gros manseng, wine

Citation

Related articles…

Evolution of the amino acids content through grape ripening: Effect of foliar application of methyl jasmonate with or without urea

The parameters that determine the grape quality, and therefore the optimal harvest time, suffer variations during berry ripening, related to climate change, with the widely known problem of the gap between technological and phenolic maturities. However, there are few studies about its incidence on grape nitrogen composition. For this reason, the use of an elicitor, methyl jasmonate (MeJ), alone or with urea, is proposed as a tool to reduce climatic decoupling, allowing to establish the harvest time in order to achieve the optimum grape quality. The aim was to study the effect of MeJ and MeJ+Urea foliar applications on the evolution of Tempranillo amino acids content throughout the grape maturation. Three treatments were foliarly applied, at veraison and 7 days later: control (water), MeJ (10 mM) and MeJ+Urea (10 mM+6 kg N/ha). Grape samples were taken at five stages of maturation: day before the first and second applications, 15 days after the second application (pre-harvest), harvest day, and 15 days after harvest (post-harvest). The amino acids analysis of the samples was carried out by HPLC. Results showed that the evolution of amino acids was similar regardless of the treatment; however, foliar applications influenced the nitrogen compounds content, i.e., there was no qualitative effect but quantitative one. Most of the amino acids reached their maximum concentration in pre-harvest, being higher in grapes from the treatments than in the control. In general, no differences in grape amino acids content were observed between MeJ and MeJ+Urea treatments. Foliar applications with MeJ and MeJ+Urea enhanced the grape amino acids content, without affecting their profile, helping to optimize their quality and allowing to establish a more complete grape ripening standard. Therefore, MeJ and MeJ+Urea foliar applications can be a simple agronomic practice, which has shown promising results in order to enhance the grape quality.

Terroir traceability in grapes, musts and wine: results of research on Gewürztraminer and Sauvignon Blanc grape varieties in northern Italy

In the study of terroir, a separate analysis of its many component factors can be of great help in accurately identifying a vineyard’s natural elements that impact wine quality and typicity. This research used a dedicated pluri-disciplinary approach to investigate the ecological characteristics, including geology and geographical features, of 14 vineyards that produce Gewürztraminer and Sauvignon Blanc cultivars in the alpine Alto Adige DOC wine region. Both the geopedological method using Vineyards Geological Identity (VGI) and the new Solar Radiaton Identity (SRI) topoclimatic classification method were used to provide analytical measurements and qualitative/quantitative characterisations. In addition, wide-ranging targeted and untargeted oenological and chemical analyses were carried out on grapes, musts and wines to correlate the soils’ geomineral and physical conditions with the biochemical properties of their fruits and wines. The research identified strong correlations between vineyard geo-identity and wine biofingerprint, confirming a mineral traceability of strontium rubidium ratio and some minerals distinctive to the local geology, such as K, Ca, Ag, Ba and Mn.  The study also discovered that particular geomineral and physical soil conditions of the studied vineyards are related to the different amount of amino acids, primary varietal aromas and polyphenols found in grapes, musts and wines. The research confirmed that winemaking technologies support oenological quality, although in some cases, human practices can overpower certain characteristic elements in wine, erasing the typical imprint left by the vineyards’ natural terroir, which becomes less traceable. Terroir abiotic ecological factors and vineyard identity can be classified in detail using the new VGI and SRI analysis methods to discover interrelationships between geo-pedological and topoclimatic conditions that impact wine quality. These methods are also helpful in identifying which ecological elements are exclusive to a particular vineyard or wine sub-region.

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Aromatic maturity is a cornerstone of terroir expression in red wine

Harvesting grapes at adequate maturity is key to the production of high-quality red wines. Enologists and wine makers define several types of maturity, including technical maturity, phenolic maturity and aromatic maturity. Technical maturity and phenolic maturity are relatively well documented in the scientific literature, while articles on aromatic maturity are scarcer. This is surprising, because aromatic maturity is, without a doubt, the most important of the three in determining wine quality and typicity (including terroir expression). Optimal terroir expression can be obtained when the different types of maturity are reached at the same time, or within a short time frame. This is more likely to occur when the ripening takes place under mild temperatures, neither too cool, nor too hot. Aromatic expression in wine can be driven, from low to high maturity, by green, herbal, fresh fruit, ripe fruit, jammy fruit, candied fruit or cooked fruit aromas. Green and cooked fruit aromas are not desirable in red wines, while the levels of other aromatic compounds contribute to the typicity of the wine in relation to its origin. Wines produced in cool climates, or on cool soils in temperate climates, are likely to express herbal or fresh fruit aromas; while wines produced under warm climates, or on warm soils in temperate climates, may express ripe fruit, jammy fruit or candied fruit aromas. Growers can optimize terroir expression through their choice of grapevine variety. Early ripening varieties perform better in cool climates and late ripening varieties in warm climates. Additionally, maturity can be advanced or delayed by different canopy management practices or training systems.