Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 First characterization of thiol precursors in colombard and gros manseng: comparison of two cultivation practices

First characterization of thiol precursors in colombard and gros manseng: comparison of two cultivation practices

Abstract

AIM: Organic production of wine in the past years has known an important augmentation. This type of cultivation practice switches synthetic phytosanitary product for copper-based protection as fungicide. While the effect of copper on soil and vine is understood, few data are published concerning its impact on wine aromas (Darriet et al. 2001) and even less concerning varietal thiol. The aim of this work was to characterize the thiol aromatic potential of Colombard and Gros Manseng grapes and to investigate the effect of copper on thiol precursor biogenesis. We selected 30 parcels (15 conventional and 15 organic) with sampling at harvest for 18 of them and 3 sampling dates during ripening for the other 12 parcels. Chemical analyses of thiol precursors were performed by adapting an UPLC-MS/MS method based upon Stable Isotope Dilution Assay (Bonnaffoux et al. 2017)

RESULTS: With this first characterization, we demonstrated that both varieties presented concentrations of glutathionylated (G3SH) and cysteinylated (Cys3SH) precursors of 3-sulfanylhexan-1-ol up to 454 µg/kg and 21 µg/kg respectively. No precursors of the 4-sulfanyl-4-methylpentan-2-one were detected. So, Colombard and Gros Manseng were ranked in the top of varietal thiol producers with Sauvignon and Pinot Gris grapes (Pena-Gallego et al. 2012). By comparing the copper protected parcels to the others, we identified a significant decrease (p-value = 0.01) of G3SH content in organic Gros Manseng grapes which was around 30%. This could represent a loss in 3SH of 1000 ng/kg in finished wines if a conversion rate of 3% between G3SH and 3SH was considered. On the opposite, Colombard grapes remained unaffected by the copper spraying with no effect on the precursors content at harvest. We also analyzed the accumulation kinetics of thiol precursors under the two protection methods (copper or not). No accumulation of thiol precursors between seven days prior and after the harvest was observed in Colombard grapes which was inconsistent with literature on other grape varieties such as Sauvignon B. or Melon B. (Roland et al. 2010). Gros Manseng grapes showed significant accumulation for conventional culture (161 µg/kg to 356 µg/kg) over the two last weeks of ripening. Furthermore, copper treated parcels of Gros Manseng have no accumulation through the two-week study. However, Pearson test did not show a direct correlation between copper content and G3SH suggesting a possible interaction of copper with thiol precursors making them not analyzable under our conditions or a modification of vine metabolism.

CONCLUSION:

We characterized for the first time two grapes varieties (Colombard and Gros Manseng) that have high potential towards varietal thiols such as Sauvignon. We identified a negative copper effect on Gros Manseng variety for both precursors content at harvest and accumulation kinetics while Colombard remained unaffected.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Gabriel Dournes, Arnaud VERBAERE, Frédéric LOPEZ, Thierry DUFOURCQ, Jean-Roch MOURET, Aurélie ROLAND

SPO, Univ Montpellier, INRAE, Institut Agro, Montpellier, France, IFV Sud-Ouest, Château de Mons, 32100 Caussens, France

Contact the author

Keywords

copper, thiol precursors, colombard, gros manseng, wine

Citation

Related articles…

Effects of organic mulches on the soil environment and yield of grapevine

Farming management practices aiming at conserving soil moisture have been developed in arid and semiarid-areas facing water scarcity problems. Organic mulching is an effective method to manipulate the crop-growing microclimate increasing crop yield by controlling soil temperature, and retaining soil moisture by reducing soil evaporation. In this sense, the effectiveness of different organic mulching materials (straw mulch and grapevine pruning debris) applied within the row of a vineyard was evaluated on the soil and on the vine in a Tempranillo vineyard located in La Rioja (Spain). Organic mulches were compared with a traditional bare soil management technique (based on the use of herbicides to avoid weed incidence). Mulching coverages favourably influenced the soil water retention throughout all the grapevine vegetative cycle. However, the soil-moisture variation was not the same under different mulching materials, being the straw mulch (SM) the one that retained more water in comparison with grapevine pruning debris (GPD) based-cover. The changes of soil moisture in the upper surface layer (0–10 cm) were highly dynamic, probably due to water vapour fluxes across the soil-atmospheric interface. However, both, SM and GPD reduced these fluctuations as compared with bare soils. A similar trend occurred with soil temperature. Both organic mulches altered soil temperature in comparison with bare soil by reducing soil temperature in summer and raising it in winter. Moreover, the same buffering effect for the temperature on the covered soil also remains in the deeper layers. To conclude, we could see that organic mulching had a positive impact on soil-moisture storage and soil temperature and the extent of this effect depends on the type of mulching materials. These changes led to higher rates of photosynthesis and stomatal conductivity compared to bare soils, also favouring crop growth and grape yields.

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Copper contamination in vineyard soils of Bordeaux: spatial risk assessment for the replanting of vines and crops

Copper (Cu) is widely and historically used in viticulture as a fungicide against mildew. Cu has a strong affinity for soil organic matter and accumulates in topsoil horizons. Thus, Cu may negatively affect soil organisms and plants, consequently reducing soil fertility and productivity. The Bordeaux vineyards have the largest vineyard surfaces (26%) within French controlled appellation and a great proportion of French wine production (around 5 million hl per year). Considering the local context of vineyard surfaces decreasing (vine uprooting) and possible new crop plantation, the issue of Cu potential toxicity rises. Therefore, the aims of this work are firstly to evaluate the Cu contamination in vineyard soils of Bordeaux, secondly to produce a risk assessment map for new vine or crop plantation. We used soil analyses from several local studies to build a database with 4496 soil horizon samples. The database was enhanced by means of pedotransfer functions in order to estimate the bioaccessible (EDTA-extractable) Cu in soils of samples without measurements. From this database, 1797 georeferenced samples with CuEDTA concentrations in the topsoil (0-50 cm depth) were used for kriging interpolation in order to produce the spatial distribution map of CuEDTA in vineyard soils. Then, the spatial distribution of Cu was crossed with vine uprooting surfaces and municipality boundaries. CuEDTAconcentrations ranged from 0.52 to 459 mg/kg and showed clear anomalies. Our results from spatial analysis showed that almost 50% of vineyard soil surfaces have CuEDTA concentrations higher than 30 mg/kg (moderate risk for new plantation) and 20% with concentrations higher than 50 mg/kg (high risk for new plantation). A decision-support map based on municipalities was realised to provide a simple tool to stakeholders concerned by land use management.

Sustainable fertilisation of the vineyard in Galicia (Spain)

Excessive fertilization of the vineyard leads to low quality grapes, increased costs and a negative impact on the environment. In order to establish an integrated management system aimed at a sustainable fertilization of the vineyards, nutritional reference levels were established. For this purpose, 30 representative vineyards of the Albariño variety were studied, in which soil and petiole analyses were carried out for two years and grape yield and quality at harvest were measured. In both years of study, soil pH, calcium, sodium and cation exchange capacity were positively correlated with calcium content and negatively correlated with manganese in grapes. Irrigated vineyards had higher levels of aluminium in soil and lower levels of calcium in petiole. Climatic conditions were very different in the years of the study. The year 2019 was colder than usual, in 2020 there was a marked water stress with high summer temperatures. This resulted in medium-high acidity in grapes in 2019 and low acidity in 2020, with sugar levels being similar both years. A very marked decrease in must amino nitrogen was observed in 2020, with ammonia nitrogen remaining stable. The correlation of acidity and sugar values in grapes with soil and petiole analysis data made it possible to establish reference levels for the nutritional diagnosis of the Albariño variety in this region. Based on these results, an easy-to-use TIC application is currently being created for grapegrowers, aimed at improving the sustainability of the vineyard through reasoned fertilization. This study has now been extended to other Galician vine varieties.

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.