Macrowine 2021
IVES 9 IVES Conference Series 9 Determination of quality related polyphenols in chilean wines by absorbance-transmission and fluorescence excitation emission matrix (a-teem) analyses

Determination of quality related polyphenols in chilean wines by absorbance-transmission and fluorescence excitation emission matrix (a-teem) analyses

Abstract

Phenolic composition is essential to wine quality (Cleary et al., 2015; Bindon et al., 2020; Niimi et al., 2020) and its assessment is a strong industrial need to quality management. Objective of this work was to develop a rapid analysis method using the Absorbance-Transmission and fluorescence Excitation-Emission Matrix (A-TEEM) technique. Polyphenols exhibit characteristic and high fluorescence quantum yields, which makes them highly suitable for this technique. The method’s automatic real-time Inner Filter Effect (IFE) correction allows the quantification of minor compounds (Gilmore et al., 2016). IFE-corrected fluorescence EEM data and the absorbance data were combined, and the spectral data were regressed against the concentrations of 34 anthocyanins, flavan-3-ols, tannins, polymeric pigments, flavonols and hydroxycinnamic acids measured independently by HPLC-DAD and UV-vis. The study focused on comparing Partial Least Squares Regression (PLSR) and Extreme Gradient Boost Regression (XGBR) for the single- (fluorescence EEM or absorbance) and multi- (combined) block data. The calibration set comprised 1133 files acquired from 126 diverse experimental and commercial wines. Validation was carried out on two data sets, first by a 14% randomized sample split from the calibration data keeping instrument replicates together, and thereafter by another independent set of 96 files from 16 wines. As a general trend, validation of the multi-block data models with independent data using XGBR, compared to PLSR, yielded higher prediction correlation coefficients (R2P) and lower Root Mean Square Errors for Prediction (RMSEP). Considering all 34 compound fits, mean R2P of 0.947 with XGBR and of 0.899 with PLSR were obtained. The highest fits were obtained for compounds of the anthocyanin family with mean R2P of 0.974 (XGBR) and 0.954 (PLSR), respectively, while lower fits were found for flavan-3-oles with R2P of 0.878 (XGBR) and 0.771 (PLSR), indicating compound effects due to extraction and chromatographic and spectral analysis methods affecting repeatability and quantification limits. In general, precise model fits were found for compounds > 10 mg/L with R2P between 0.929 and 0.992 (XGBR) and between 0.875 and 0.992 (PLSR). Supplementary, all individual compounds could be identified according to their family by spectral fingerprints. However, these multi-block data sets were also associated with significantly higher R2P (and lower RMSEP) compared to a single block evaluation of the fluorescence EEM or absorbance data only. By using mean-centering and an Extended Mixture Model filter the multi-block data sets fit robustly using both XGBR and PLSR without the need to apply secondary variable selection algorithms. We conclude that analyzing the A-TEEM data using the multi-block organization and the XGBR algorithm facilitates a robust prediction of the key phenolic compound concentrations that strongly influence the Chilean wine quality.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Doreen Schober

Center for Research and Innovation, Viña Concha y Toro, Ruta k-650 km 10, Pencahue, Región de Maule, Chile,Adam Gilmore, HORIBA Instruments Inc. 20 Knightsbridge Rd., Piscataway, NJ 08854, USA Jorge Zincker, Center for Research and Innovation, Viña Concha y Toro, Ruta k-650 km 10, Pencahue, Región de Maule, Chile Alvaro Gonzalez, Center for Research and Innovation, Viña Concha y Toro, Ruta k-650 km 10, Pencahue, Región de Maule, Chile

Contact the author

Keywords

quality, polyphenols, spectroscopy, a-teem, wine, machine learning

Citation

Related articles…

Effect of regulated deficit irrigation regime on amino acids content of Monastrell (Vitis vinifera L.) grapes

Irrigation is an important practice to influence vine quality, especially in Mediterranean regions, characterized by hot summers and severe droughts during the growing season. This study focused on deficit irrigation regime influence on amino acids composition of Monastrell grapevines under semiarid conditions (Albacete, Southeastern of Spain). In 2019, two treatments were applied: non-irrigation (NI) and regulated deficit irrigation (RDI), watered at 30% of the estimated crop evapotranspiration from fruit set to onset of veraison. Grape amino acids content was analyzed by HPLC. Berries from non-irrigated vines showed higher concentration of several amino acids, such as tryptophan (73%), arginine (70%), lysine (36%), isoleucine (27%), and leucine (21%), compared to RDI grapes. Arginine is, together with ammonium ion, the principal nitrogen source for yeasts during the alcoholic fermentation; while isoleucine, tryptophan, and leucine are precursors of fermentative volatile compounds, key compounds for wine quality. Moreover, NI treatment increased in a 14% the total amino acids content in grapes compared to RDI treatment. The reported effects might be because yield was 70% higher in RDI vines than in the NI ones and, therefore, the sink demand was increased in the irrigated vines. In addition, NI vines suffered more severe water stress and it is known that the amino acids synthesis and accumulation can be influenced by the plant response to stress. According to the results, the irrigation regime showed effect on amino acids concentration in Monastrell grapes under semiarid conditions. Grapes from non-irrigated vines showed a higher content of several amino acids relevant to the fermentative process and to the wine aroma compounds formation. It is demonstrated that the final content of nitrogen-related components in grapes is influenced by the irrigation regime. The convenience of the irrigation strategy to suggest will depend on the desired wine style and the target yield levels.

Evaluation of climate change impacts at the Portuguese Dão terroir over the last decades: observed effects on bioclimatic indices and grapevine phenology

In the last decades the growers of the Portuguese Dão winegrowing region (center of Portugal) are experiencing changes in climate that are influencing either grape phenology berry health and ripening. Aiming to study the relationships between climate indices (CI), seasonal weather and grapevine phenology, in this work long-term climate and phenological data collected at the experimental vineyard of the Portuguese Dão research centre between 1958 and 2019 (61 years) for the red variety Touriga Nacional, was analyzed. The trends over time for the classical temperature-based indices (Growing Season Temperature – GST -, Growing Degree Days – GDD, Huglin Index – HI and Cool Night Index – CI) presented a significantly positive slope while the Dryness Index (DI) showed a negative trend over the last 61 years. Regarding grapevine phenology, an average advance of 4.5 days per decade in the harvest day was observed throughout the last 61 years. Consequently, the weather conditions during the ripening period have changed, showing an increasing trend over time in the average temperature (higher magnitude in the maximum than in the minimum temperature) and a decrease in the accumulated rainfall. A regression analysis showed that ~50% of harvest date variability over years was explained by the temperature-based indices variability. These observed effects of climate change on bioclimatic indices and corresponding anticipation of harvest date can still be considered advantageous for the Dão terroir as it allows to achieve an optimal berry ripening before the common equinox rains and, therefore, avoid the potential negative impacts of the rainfall on berry health and composition.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Copper contamination in vineyard soils of Bordeaux: spatial risk assessment for the replanting of vines and crops

Copper (Cu) is widely and historically used in viticulture as a fungicide against mildew. Cu has a strong affinity for soil organic matter and accumulates in topsoil horizons. Thus, Cu may negatively affect soil organisms and plants, consequently reducing soil fertility and productivity. The Bordeaux vineyards have the largest vineyard surfaces (26%) within French controlled appellation and a great proportion of French wine production (around 5 million hl per year). Considering the local context of vineyard surfaces decreasing (vine uprooting) and possible new crop plantation, the issue of Cu potential toxicity rises. Therefore, the aims of this work are firstly to evaluate the Cu contamination in vineyard soils of Bordeaux, secondly to produce a risk assessment map for new vine or crop plantation. We used soil analyses from several local studies to build a database with 4496 soil horizon samples. The database was enhanced by means of pedotransfer functions in order to estimate the bioaccessible (EDTA-extractable) Cu in soils of samples without measurements. From this database, 1797 georeferenced samples with CuEDTA concentrations in the topsoil (0-50 cm depth) were used for kriging interpolation in order to produce the spatial distribution map of CuEDTA in vineyard soils. Then, the spatial distribution of Cu was crossed with vine uprooting surfaces and municipality boundaries. CuEDTAconcentrations ranged from 0.52 to 459 mg/kg and showed clear anomalies. Our results from spatial analysis showed that almost 50% of vineyard soil surfaces have CuEDTA concentrations higher than 30 mg/kg (moderate risk for new plantation) and 20% with concentrations higher than 50 mg/kg (high risk for new plantation). A decision-support map based on municipalities was realised to provide a simple tool to stakeholders concerned by land use management.