Macrowine 2021
IVES 9 IVES Conference Series 9 Use of membrane ultrafiltration technology to achieve protein stabilisation of white wine

Use of membrane ultrafiltration technology to achieve protein stabilisation of white wine

Abstract

AIM: Proteins in white wine can cause cloudiness or haze after bottling, which consumers may consider an indicator of poor quality. As a consequence, winemakers often use bentonite, a clay-based material that binds protein, to remove proteins and achieve protein stabilisation. However, removing bentonite from wine after treatment can result in a 3-10% loss of wine (1). Membrane filtration technology is used in wine production for many purposes and ultrafiltration (UF) offers an easily-translatable process for protein removal (1). UF treatment of wine can produce heat-stable permeate and protein-enriched retentate, which enables targeted protein degradation. Heating the retentate, with or without protease significantly improved the heat stability of recombined wine in pilot scale trials (2). This study evaluated strategies for achieving protein stabilisation using membrane filtration.

METHODS: Sauvignon blanc wine (unfined) was fractionated by UF in triplicate, the resulting retentate subjected to protease and heat (62℃, 10 min) treatment, and the treated retentate recombined with the permeate. Traditional bentonite fining was performed as a positive control. Chemical and sensory analyses were carried out to evaluate the efficacy of treatment.

RESULTS: Heating retentate with protease reduced the concentration of haze-forming proteins by 54% compared with heating alone 40%. Chemical analyses and quality scores for recombined wine showed no significant difference with bentonite-fined wines. Sensory analysis indicated that UF/heat-treatment increased the green apple aroma, alcohol heat and overall flavour intensity of the wines compared to bentonite fined wines, suggesting UF-treated wines retained flavour without imparting oxidative characters.

CONCLUSIONS

Ultrafiltration combined with heat and protease treatment can reduce bentonite use without significantly affecting sensory properties. While results are promising, it is not yet a viable alternative to bentonite fining.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Yihe Sui

The University of Adelaide, School of Agriculture, Food and Wine; Australian Research Council Training Centre for Innovative Wine Production.,David, WOLLAN, VAF Memstar; Australian Research Council Training Centre for Innovative Wine Production –      Jacqui, MCRAE, The University of Adelaide, School of Chemical Engineering and Advanced Materials – Richard, MUHLACK, The University of Adelaide, School of Agriculture, Food and Wine; Australian Research Council Training Centre for Innovative Wine Production – Peter, GODDEN, The Australian Wine Research Institute –            Kerry, WILKINSON, The University of Adelaide, School of Agriculture, Food and Wine; Australian Research Council Training Centre for Innovative Wine Productin

Contact the author

Keywords

white wine heat stability, haze, ultrafiltration, wine protein, protease

Citation

Related articles…

Adaptation to climate change by determining grapevine cultivar differences using temperature-based phenology models

Grapevine phenology is advancing with increased temperatures associated with climate change. This may result in higher fruit sugar concentrations at harvest and/or earlier compressed harvests and changes in the synchrony of sugar with other fruit metabolites. One adaptation strategy that growers may use to maintain typicity of wine style is to change cultivars. This approach may enable fruit

Reviewing the geometry of terraces in the Douro region towards sustainable viticulture

The Douro demarcated region constitutes just over 50% of the area of mountain vineyard in the world, i.e., vineyards with slope gradients of 30% or above. Among the different (terraced) vineyard layouts, the formerly preferred wider terraces supporting two rows of vines and the currently advocated narrower single vine row, dominate the vineyards’ planting layout. The slope of these terraces, in other words, the supporting earth ramp, is a key element in these vineyards’ construction.

La hiérarchisation des Coteaux du Languedoc: une application concrète du zonage vitivinicole

L’A.O.C. Coteaux du Languedoc est située dans le Sud de la France, dans la partie Ouest de la bordure méditerranéenne. Elle forme un vaste amphithéâtre largement ouvert sur la mer méditerranée. L’Appellation a été constituée en 1960 par le regroupement de 14 anciennes petites appellations d’origine représentant 55 communes éparpillées dans les départements de l’Aude, de l’Hérault et du Gard. Par la suite, plusieurs extensions successives ont conduit à un ensemble actuellement composé de 168 communes.

Modulating role of SO2 in white wine protein haze formation

Despite the extensive research performed during the last decades, the multifactorial mechanism responsible for the white wine protein haze formation is not fully characterized. Herein, a new model is proposed, which is based on the experimental identification of sulfur dioxide as a major modulating factor inducing wine protein haze upon heating. As opposed to other reducing agents, such as 2-mercaptoethanol, dithiothreitol and tris(2-carboxyethyl)phosphine hydrochloride (TCEP), the addition of SO2 to must/wine upon heating cleaves intraprotein disulfide bonds, hinders thiol-disulfide exchange during protein interactions and can lead to the formation of novel inter/intraprotein disulfide bonds. Those are eventually responsible for wine protein aggregation which follows a nucleation-growth kinetic model as shown by dynamic light scattering [1].

Beneficial effects of moderate consumption of Teran red wine on blood lipid profile: a preliminary study on healthy volunteers

Moderate wine consumption may impact several human health aspects, among others as a result of phenolic compounds present in wine and their bioactive properties. The aim of this study was to determine whether six weeks of daily, moderate Teran red wine consumption affects the levels of total cholesterol, high-density lipoprotein cholesterol – HDL, low-density lipoprotein cholesterol – LDL, and triglycerides. Sixty-eight healthy adults (46 women and 22 men) aged 25-64 years voluntarily agreed to participate in the study.