Macrowine 2021
IVES 9 IVES Conference Series 9 High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

Abstract

AIM: High pressure technologies represent a promising alternative to thermal treatments for improving quality and safety of liquid foods. High Hydrostatic Pressure (HHP), High Pressure Homogenization (HPH) and Ultra-High Pressure Homogenization (UHPH) are gaining increasing interest in wine industry, for their ability to inactivate microorganisms [1-3], improve the extraction of color and phenolic compounds from grapes [4,5] and to induce yeast autolysis [6] potentially accelerating wine ageing on lees (AOL). This work aims at evaluating the possibility of accelerating AOL of white wines by HPH processing of fermentation lees, considering the effects of the treatment on microbial populations, wine composition, sensory and aroma profile, as well as the potential impact on wine filterability.

METHODS: Lees were collected at the end of alcoholic fermentation (fresh lees) and after six months of ageing (aged lees) and processed by HPH at 60 and 150 MPa (1 and 2 passes). The effects on microbial populations and the release of polysaccharides were evaluated in comparison with untreated samples and β-glucanase addition. The modifications induced on yeast cells were also investigated by Transmission Electronic Microscopy. Treated lees were added (5 % v/v) to a white wine and samples were analyzed after one and six months of AOL, concerning polysaccharide content, microbial composition, basic chemical parameters, aroma and sensory profile. Finally, to assess the impact of HPH on wine filterability, the Particle Size Distribution of colloidal particles and a filtration test were determined at the end of ageing period.

RESULTS: HPH favored the release of polysaccharides from lees, with a higher efficiency if lees are treated immediately after alcoholic fermentation (fresh lees), revealing to be averagely more efficient than β-glucanase enzymes. HPH also determined a significant reduction of viable yeasts and lactic bacteria in treated lees, potentially allowing to reduce the use of sulfur dioxide during AOL; the effects on microorganisms were dependent on the pressure applied and the number of passes. High pressure treatments provoked a complete disruption of yeast cells, forming cell debris with a greater particle size with respect to what detected in untreated samples or in the lees treated with enzymes. This determined the formation of a persistent haze in lees samples. The effect of this particles on wine filterability was negligible if the pressure applied during lees treatment was low, but filtration became more difficult as operating pressure and number of passes increased.

CONCLUSIONS

High pressure techniques represent an interesting perspective for the application investigated in the present study. The possibility of their exploitation at winery scale requires the identification of suitable operating conditions and the evaluation of the economic aspects connected with their scale-up at industrial level.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Piergiorgio Comuzzo

Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy),Sabrina VOCE Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Lucilla IACUMIN Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Rita MUSETTI Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Gabriele CHINNI Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Giovanni CARRANO Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Marco MARCONI JU.CLA.S. S.r.l., via Mirandola 49/A, 37026 Settimo di Pescantina (VR), Italy  Gianmaria ZANELLA Enologica Vason S.p.A., via Nassar 37, 37029 San Pietro in Cariano (VR), Italy

Contact the author

Keywords

hph; emerging technologies; ageing on lees; microbial inactivation; wine polysaccharides; sulfur dioxide decrease; filtration

Citation

Related articles…

Adapting the vineyard to climate change in warm climate regions with cultural practices

Since the 1980s global regime shift, grape growers have been steadily adapting to a changing climate. These adaptations have preserved the region-climate-cultivar rapports that have established the global trade of wine with lucrative economic benefits since the middle of 17th century. The advent of using fractions of crop and actual evapotranspiration replacement in vineyards with the use of supplemental irrigation has furthered the adaptation of wine grape cultivation. The shift in trellis systems, as well as pruning methods from positioned shoot systems to sprawling canopies, as well as adapting the bearing surface from head-trained, cane-pruned to cordon-trained, spur-pruned systems have also aided in the adaptation of grapevine to warmer temperatures. In warm climates, the use of shade cloth or over-head shade films not only have aided in arresting the damage of heat waves, but also identified opportunities to reduce the evapotranspiration from vineyards, reducing environmental footprint of vineyard. Our increase in knowledge on how best to understand the response of grapevine to climate change was aided with the identification of solar radiation exposure biomarker that is now used for phenotyping cultivars in their adaptability to harsh environments. Using fruit-based metrics such as sugar-flavonoid relationships were shown to be better indicators of losses in berry integrity associated with a warming climate, rather than solely focusing on region-climate-cultivar rapports. The resilience of wine grape was further enhanced by exploitation of rootstock × scion combinations that can resist untoward droughts and warm temperatures by making more resilient grapevine combinations. Our understanding of soil-plant-atmosphere continuum in the vineyard has increased within the last 50 years in such a manner that growers are able to use no-till systems with the aid of arbuscular mycorrhiza fungi inoculation with permanent cover cropping making the vineyard more resilient to droughts and heat waves. In premium wine grape regions viticulture has successfully adapted to a rapidly changing climate thus far, but berry based metrics are raising a concern that we may be approaching a tipping point.

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

How distinctive are single vineyard Gewürztraminer musts and wines from Alto Adige (Italy) based on untargeted analysis, sensory profiling, and chemometric elaboration?

Vitis vinifera L. ‘Gewürztraminer’ is a historical grape variety of Alto Adige (Südtirol), Italy, which is widely grown in the area of Tramin an der Weinstraße, but is also grown globally. It produces highly aromatic wines that are strongly influenced by the terroir of the vineyard sites where they are grown. This study looked at musts and young wines from ‘Gewürztraminer’ grapes harvested in seven distinct vineyards near Tramin and then processed at Cantina di Termeno, minimizing winemaking protocol variability. Samples were profiled using bidimensional gas chromatography–time-of-flight mass spectrometry, liquid chromatography coupled to electrochemical detection, and near-IR spectrometry. The data were subjected to Principle Component Analysis and Hierarchical Clustering Analysis. Sensory discriminant testing was undertaken using the sorting method with a semi-trained panel, and the data were processed using Multidimensional Scaling. Seven must/wine pairs could be distinguished based on their untargeted volatilome profiles and on sensory evaluation. As expected, there were greater differences in the volatile compounds between the wines than between the musts. The wines from vineyards 4 and 5 were nonetheless quite homogenous in terms of chemical and sensory analyses, as were the wines from vineyards 1 and 3. For the phenolic profile, differences were noted between the musts and wines of vineyards 2, 3, and 4, but the musts from vineyards 5 and 7 were similar. Sensory analysis showed the wines from vineyards 6 and 7 to be distinct from the rest. These results reinforce that the composition of ‘Gewürztraminer’ musts and wines is strongly determined by vineyard site, even in a small geographic area with high variability of the terroir (soil and microclimate), and that these differences are apparent in the flavours and aromas of the finished wines. Further confirmation would require a larger sample of wines, preferably from several vintages.