Macrowine 2021
IVES 9 IVES Conference Series 9 High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

Abstract

AIM: High pressure technologies represent a promising alternative to thermal treatments for improving quality and safety of liquid foods. High Hydrostatic Pressure (HHP), High Pressure Homogenization (HPH) and Ultra-High Pressure Homogenization (UHPH) are gaining increasing interest in wine industry, for their ability to inactivate microorganisms [1-3], improve the extraction of color and phenolic compounds from grapes [4,5] and to induce yeast autolysis [6] potentially accelerating wine ageing on lees (AOL). This work aims at evaluating the possibility of accelerating AOL of white wines by HPH processing of fermentation lees, considering the effects of the treatment on microbial populations, wine composition, sensory and aroma profile, as well as the potential impact on wine filterability.

METHODS: Lees were collected at the end of alcoholic fermentation (fresh lees) and after six months of ageing (aged lees) and processed by HPH at 60 and 150 MPa (1 and 2 passes). The effects on microbial populations and the release of polysaccharides were evaluated in comparison with untreated samples and β-glucanase addition. The modifications induced on yeast cells were also investigated by Transmission Electronic Microscopy. Treated lees were added (5 % v/v) to a white wine and samples were analyzed after one and six months of AOL, concerning polysaccharide content, microbial composition, basic chemical parameters, aroma and sensory profile. Finally, to assess the impact of HPH on wine filterability, the Particle Size Distribution of colloidal particles and a filtration test were determined at the end of ageing period.

RESULTS: HPH favored the release of polysaccharides from lees, with a higher efficiency if lees are treated immediately after alcoholic fermentation (fresh lees), revealing to be averagely more efficient than β-glucanase enzymes. HPH also determined a significant reduction of viable yeasts and lactic bacteria in treated lees, potentially allowing to reduce the use of sulfur dioxide during AOL; the effects on microorganisms were dependent on the pressure applied and the number of passes. High pressure treatments provoked a complete disruption of yeast cells, forming cell debris with a greater particle size with respect to what detected in untreated samples or in the lees treated with enzymes. This determined the formation of a persistent haze in lees samples. The effect of this particles on wine filterability was negligible if the pressure applied during lees treatment was low, but filtration became more difficult as operating pressure and number of passes increased.

CONCLUSIONS

High pressure techniques represent an interesting perspective for the application investigated in the present study. The possibility of their exploitation at winery scale requires the identification of suitable operating conditions and the evaluation of the economic aspects connected with their scale-up at industrial level.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Piergiorgio Comuzzo

Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy),Sabrina VOCE Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Lucilla IACUMIN Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Rita MUSETTI Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Gabriele CHINNI Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Giovanni CARRANO Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Marco MARCONI JU.CLA.S. S.r.l., via Mirandola 49/A, 37026 Settimo di Pescantina (VR), Italy  Gianmaria ZANELLA Enologica Vason S.p.A., via Nassar 37, 37029 San Pietro in Cariano (VR), Italy

Contact the author

Keywords

hph; emerging technologies; ageing on lees; microbial inactivation; wine polysaccharides; sulfur dioxide decrease; filtration

Citation

Related articles…

Biodiversity in the vineyard agroecosystem: exploring systemic approaches

Biodiversity conservation and restoration are essential for guarantee the provision of ecosystem services associated to vineyard agroecosystem such as climate regulation trough carbon sequestration and control of pests and diseases. Most of published research dealing with the complexity of the vineyard agroecosystems emphasizes the necessity of innovative approaches, including the integration of information at different temporal and spatial scales and development of systemic analysis based on modelling. A biodiversity survey was conducted in the Franciacorta wine-growing area (Lombardy, Italy), one of the most important Italian wine-growing regions for sparkling wine production, considering a portion of the territory of 112 ha. The area was divided into several Environmental Units (EUs), defined as a whole vineyard or portion of vineyard homogenous in terms of four agronomic characteristics: planting year, planting density, cultivar, and training system. In each EU a set of compartments was identified and characterised by specific variables. The compartments are meteorology, morphology (altitude, slope, aspect, row orientation, and solar irradiance), ecological infrastructures and management. The landscape surrounding EU was also characterised in terms of land-use in a buffer zone of 500 m. For each component a specific methodology was identified and applied. Different statistical approaches were used to evaluate the method to integrate the information related to different compartments within the EU and related to the buffer zone. These approaches were also preliminarily evaluated for their ability to describe the contribution of biodiversity and landscape components to ecosystem services. This methodological exploration provides useful indication for the development of a fully systemic approach to structural and functional biodiversity in vineyard agroecosystems, contributing to promote a multifunctional perspective for the all wine-growing sector.

Grapevine varietal diversity as mitigation tool for climate change: Agronomic and oenologic potential of 14 foreign varieties grown in Languedoc region (France)

Climate change effects in Languedoc include an expected rise in temperatures, increased evapotranspiration as well as more severe and frequent climatic hazards, such as frost, drought periods and heat waves. For winegrowers theses phenomena impact both yield and quality, resulting in more frequent unbalanced wines. Research on identified mitigation tools for vineyard management is necessary to improve resilience of grapevine agrosystems. Varietal assortment is one of them. This study focuses on agronomic and oenologic potential of 14 foreign varieties grown in Languedoc French region. Fourteen grapevine varieties were monitored during 2021 from June until harvest on eight different sites, some of which occurring on more than one site adding up to 21 different modalities: 7 white varieties Alvarinho B, Assyrtiko B (2), Malvasia Istriana B, Parellada B, Verdejo B, Verdelho B, Xarello B, and 7 black varieties Saperavi N (2), Touriga nacional N, Baga N, Aleatico N, Montepulciano N (2), Primitivo N (3), Calabrese N (3). Varietals were compared through the following parameters: phenology was assessed by using the information collected in the Database Network of French Vine Conservatories (INRAE-SupAgro-IFV, 2005-2015). The number of inflorescences for shoots from secondary buds and bourillons and suckers were observed to assess post-bud break frost tolerance potential. Grapevine water status was studied through stem water potential measurement, observation of foliage symptoms of drought, and 𝛿13C on must. Frequencies and intensities of downy mildew, powdery mildew, and black rot attacks were estimated before harvest on leaves and clusters and botrytis at harvest to assess disease susceptibilities. Berry composition was monitored from end of veraison until harvest. Yield and mean bunch weight were also calculated. Varieties were then ranked on a 1-4 scale for each parameter and compared through PCA. Forty two stations of the Mediterranean basin were compared by PCA with the Multicriteria Climatic Classification indicators in order to confront the collected information during 2021 campaign to the hypothesis that plants coming from dry and hot regions are genetically adapted to such climatic conditions.

VINIoT – Precision viticulture service

The project VINIoT pursues the creation of a new technological vineyard monitoring service, which will allow companies in the wine sector in the SUDOE space to monitor plantations in real time and remotely at various levels of precision. The system is based on spectral images and an IoT architecture that allows assessing parameters of interest viticulture and the collection of data at a precise scale (level of grape, plant, plot or vineyard) will be designed. In France, three subjects were specifically developed: evaluation of maturity, of water stress, and detection of flavescence dorée. For the evaluation of maturity, it has been decided first to work at the berry scale in the laboratory, then at the bunch scale and finally in the vineyard. The acquisition of the spectral hyperstal image as well as the reference analyzes to measure the maturity, were carried out in the laboratory after harvesting the berries in a maturity monitoring context. This work focuses on a case study to predict sugar content of three different grape varieties: Syrah, Fer Servadou and Mauzac. A robust method called Roboost-PLSR, developed in the framework of this work (Courand et al., 2022), to improve prediction model performance was applied on spectra after the acquirement of hyperspectral images. Regarding the evaluation of water stress, to work with a significant variability in terms of water status, it has been worked first with potted plants under 2 different water regimes. The facilities have allowed the supervision of irrigation and micro-climatic conditions. The regression models on agronomic variables (stomatal conductance, water potential, …) are studied. To detect flavescence dorée, the experimental plan has consisted of work at leaf scale in the laboratory first, and then in the field. To detect the disease from hyper-spectral imaging, a combination of multivariate curve resolution-alternating least squares (MCR-ALS) and factorial discriminant analysis (FDA) was proposed. This strategy proved the potential towards the discrimination of healthy and infected leaves by flavescence dorée based on the use of hyperspectral images (Mas Garcia et al., 2021).

Grape berry size is a key factor in determining New Zealand Pinot noir wine composition

Making high quality but affordable Pinot noir (PN) wine is challenging in most terroirs and New Zealand’s (NZ) situation is no exception. To increase the probability of making highly typical PN wines producers choose to grow grapes in cool climates on lower fertility soils while adopting labour intensive practices. Stringent yield targets and higher input costs necessarily mean that PN wine cost is high, and profitability lower, in line-priced varietal wine ranges. To understand the reasons why higher yielding vines are perceived to produce wines of lower quality we have undertaken an extensive study of PN in NZ. Since 2018, we established a network of twelve trial sites in three NZ regions to find individual vines that produced acceptable commercial yields (above 2.5kg per vine) and wines of composition comparable to “Icon” labels. Approximately 20% of 660 grape lots (N = 135) were selected from within a narrow juice Total Soluble Solids (TSS) range and made into single vine wines under controlled conditions. Principal Component Analysis of the vine, berry, juice and wine parameters from three vintages found grape berry mass to be most effective clustering variable. As berry mass category decreased there was a systematic increase in the probability of higher berry red colour and total phenolics with a parallel increase in wine phenolics, changed aroma fraction and decreased juice amino acids. The influence of berry size on wine composition would appear stronger than the individual effects of vintage, region, vineyard or vine yield. Our observations support the hypothesis that it is possible to produce PN wines that fall within an “Icon” benchmark composition range at yields above 2.5kg per vine provided that the Leaf Area:Fruit Weight ratio is above 12cm2 per g, mean berry mass is below 1.2g and juice TSS is above 22°Brix.

Copper contamination in vineyard soils of Bordeaux: spatial risk assessment for the replanting of vines and crops

Copper (Cu) is widely and historically used in viticulture as a fungicide against mildew. Cu has a strong affinity for soil organic matter and accumulates in topsoil horizons. Thus, Cu may negatively affect soil organisms and plants, consequently reducing soil fertility and productivity. The Bordeaux vineyards have the largest vineyard surfaces (26%) within French controlled appellation and a great proportion of French wine production (around 5 million hl per year). Considering the local context of vineyard surfaces decreasing (vine uprooting) and possible new crop plantation, the issue of Cu potential toxicity rises. Therefore, the aims of this work are firstly to evaluate the Cu contamination in vineyard soils of Bordeaux, secondly to produce a risk assessment map for new vine or crop plantation. We used soil analyses from several local studies to build a database with 4496 soil horizon samples. The database was enhanced by means of pedotransfer functions in order to estimate the bioaccessible (EDTA-extractable) Cu in soils of samples without measurements. From this database, 1797 georeferenced samples with CuEDTA concentrations in the topsoil (0-50 cm depth) were used for kriging interpolation in order to produce the spatial distribution map of CuEDTA in vineyard soils. Then, the spatial distribution of Cu was crossed with vine uprooting surfaces and municipality boundaries. CuEDTAconcentrations ranged from 0.52 to 459 mg/kg and showed clear anomalies. Our results from spatial analysis showed that almost 50% of vineyard soil surfaces have CuEDTA concentrations higher than 30 mg/kg (moderate risk for new plantation) and 20% with concentrations higher than 50 mg/kg (high risk for new plantation). A decision-support map based on municipalities was realised to provide a simple tool to stakeholders concerned by land use management.