Macrowine 2021
IVES 9 IVES Conference Series 9 High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

Abstract

AIM: High pressure technologies represent a promising alternative to thermal treatments for improving quality and safety of liquid foods. High Hydrostatic Pressure (HHP), High Pressure Homogenization (HPH) and Ultra-High Pressure Homogenization (UHPH) are gaining increasing interest in wine industry, for their ability to inactivate microorganisms [1-3], improve the extraction of color and phenolic compounds from grapes [4,5] and to induce yeast autolysis [6] potentially accelerating wine ageing on lees (AOL). This work aims at evaluating the possibility of accelerating AOL of white wines by HPH processing of fermentation lees, considering the effects of the treatment on microbial populations, wine composition, sensory and aroma profile, as well as the potential impact on wine filterability.

METHODS: Lees were collected at the end of alcoholic fermentation (fresh lees) and after six months of ageing (aged lees) and processed by HPH at 60 and 150 MPa (1 and 2 passes). The effects on microbial populations and the release of polysaccharides were evaluated in comparison with untreated samples and β-glucanase addition. The modifications induced on yeast cells were also investigated by Transmission Electronic Microscopy. Treated lees were added (5 % v/v) to a white wine and samples were analyzed after one and six months of AOL, concerning polysaccharide content, microbial composition, basic chemical parameters, aroma and sensory profile. Finally, to assess the impact of HPH on wine filterability, the Particle Size Distribution of colloidal particles and a filtration test were determined at the end of ageing period.

RESULTS: HPH favored the release of polysaccharides from lees, with a higher efficiency if lees are treated immediately after alcoholic fermentation (fresh lees), revealing to be averagely more efficient than β-glucanase enzymes. HPH also determined a significant reduction of viable yeasts and lactic bacteria in treated lees, potentially allowing to reduce the use of sulfur dioxide during AOL; the effects on microorganisms were dependent on the pressure applied and the number of passes. High pressure treatments provoked a complete disruption of yeast cells, forming cell debris with a greater particle size with respect to what detected in untreated samples or in the lees treated with enzymes. This determined the formation of a persistent haze in lees samples. The effect of this particles on wine filterability was negligible if the pressure applied during lees treatment was low, but filtration became more difficult as operating pressure and number of passes increased.

CONCLUSIONS

High pressure techniques represent an interesting perspective for the application investigated in the present study. The possibility of their exploitation at winery scale requires the identification of suitable operating conditions and the evaluation of the economic aspects connected with their scale-up at industrial level.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Piergiorgio Comuzzo

Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy),Sabrina VOCE Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Lucilla IACUMIN Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Rita MUSETTI Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Gabriele CHINNI Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Giovanni CARRANO Università degli Studi di Udine – Dipartimento di Scienze Agroalimentari, Ambientali e Animali, via Sondrio, 2/A, 33100, Udine (Italy)  Marco MARCONI JU.CLA.S. S.r.l., via Mirandola 49/A, 37026 Settimo di Pescantina (VR), Italy  Gianmaria ZANELLA Enologica Vason S.p.A., via Nassar 37, 37029 San Pietro in Cariano (VR), Italy

Contact the author

Keywords

hph; emerging technologies; ageing on lees; microbial inactivation; wine polysaccharides; sulfur dioxide decrease; filtration

Citation

Related articles…

Climate modeling at local scale in the Waipara winegrowing region in the climate change context

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.

Climate change projections to support the transition to climate-smart viticulture

The Earth’s system is undergoing major changes through a wide range of spatial and temporal scales as a response to growing anthropogenic radiative forcing, which is pushing the whole system far beyond its natural variability. Sources of greenhouse gases largely exceed their sinks, thus leading to a strengthened greenhouse effect. More energy is thereby being supplied to the system, with inevitable shifts in climatic patterns and weather regimes. Over the last decades, these modifications have been manifested in the full statistical distributions of the atmospheric variables, with dramatic changes in the frequency and intensity of extremes. Natural hazards, such as severe droughts, floods, forest fires, or heatwaves, are being triggered by extreme atmospheric events worldwide, thus threatening human activities. Viticultculture is not only exposed to changing climates but is also highly vulnerable, as grapevine phenology and physiological development are strongly controlled by atmospheric conditions. Therefore, the assessment of climate change projections for a given region is critical for climate change adaptation and risk reduction in viticulture. By adopting timely and suitable measures, the future sustainability and resiliency of the sector can be fostered. Climate-grapevine chain modelling is an essential tool for better planning and management. However, the accuracy of the resulting projections is limited by many uncertainties that must be duly taken into account when transferring knowledge to stakeholders and decision-makers. Climate-smart viticulture will comprise ensembles of locally tuned strategies, envisioning both adaptation and mitigation, assisted by emerging technologies and decision-support systems.

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.