Macrowine 2021
IVES 9 IVES Conference Series 9 Assay of distinct modes of polysaccharidases dosage in vinification with cv. Malbec. Effects on microbiological evolution, color and skin depletion

Assay of distinct modes of polysaccharidases dosage in vinification with cv. Malbec. Effects on microbiological evolution, color and skin depletion

Abstract

In the maceration stage of winemaking, enzymes can be used to degrade the polysaccharides present in the cell walls and middle sheets, and thus facilitate the extraction of juice and the release of polyphenols and aroma precursors retained in the grape skins. This work aims to analyze the influence of two enzyme complexes produced by autochthonous yeasts on the red winemaking process, in order to evaluate their effect on the chemical composition of the wines obtained, as well as on the extraction of color and polyphenols, and the depletion of the skin. Two strains previously selected for the effect of their enzymatic complex on the color extraction and improvement in the technological properties of the grape must were used (Longhi et al., 2019). A multi-enzymatic extract from Aureobasidium pullulans m11-2 was obtained by inoculating the microorganism in a broth according to Moyo et al. (2003) with modifications (pH 3.8) and incubated with stirring at 28°C for 72 h. Pectinase, xylanase, cellulase and amylase activities were quantified by determining reducing sugars by DNS, modified by Qian Li et al. (2015). Likewise, Torulaspora delbrueckii m7-2 was used for the production of the enzyme complex during vinification. Malbec red grapes (Vitis vinifera L.) from San Rafael (Mendoza) wine region, vintage 2021, were used to conduct the vinifications. The must obtained by crushing 60 Kg of grapes was corrected in acidity, sulfited (50 ppm) and distributed in 5 L containers. Four winemaking assays were performed, in duplicate: (1) inoculation with a native strain of Sacchromyces cerevisiae (SR1), at 108 cell/mL as inoculum, conducted at 20°C (control, C); (2) sequential inoculation of T. delbrueckii m7-2, with an initial cellular concentration of 107 cells/mL, followed by SR1 inoculation at 4th day (Td); (3) cold pre-fermentation maceration (CPM, 8°C-4 days) with m11-2 enzyme extract and SR1 inoculation (Ap); and (4) CPM without enzymatic treatment and SR1 inoculation (E). Growth kinetics of total yeasts were determined on YPD and DRBC agar, and of non-Saccharomyces yeasts in lysine medium. All enzymatic activities were monitored at pH 3.80 and 20°C. The pectinolytic activity was the main one, showing a level of 1.80 U/mL in the m11-2 extract and an initial level of 1.47 U/mL for the in situ producer strain (m7-2). Microscopic observations of the extracted skins in Ap and E vinifications were carried out to evaluate the effect of the enzymatic complex m11-2 on the cell wall, and were also compared with the fresh grape skins. Differences were observed between the skins enzymatically treated (Ap) and the control (E); the former showed cell emptying, greater rupture of the epidermis layers and less firmness, unlike the control that exhibited almost intact epidermal layers. These images allowed us to know the cell morphology of the varietal cv. Malbec and the enzymatic hydrolysis of its cell walls.

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Sara Jaquelina Longhi 

 Biotechnology Laboratory, Department of Biology and Food, Faculty of Sciences Applied to Industry, National University of Cuyo. Bernardo de Irigoyen 375, San Rafael, Mendoza, Argentina. National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Autonomous City of Buenos Aires, Argentina. ,María Carolina MARTÍN (1,2)  María Belén AVENDAÑO(1) María Gabriela MERÍN (1,2)   Luciana Paola PRENDES (1,2) Juliana GARAU (1,2) Vilma Inés MORATA DE AMBROSINI (1,2) (1) Biotechnology Laboratory, Department of Biology and Food, Faculty of Sciences Applied to Industry, National University of Cuyo. Bernardo de Irigoyen 375, San Rafael, Mendoza, Argentina. (2) National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Autonomous City of Buenos Aires, Argentina

Contact the author

Keywords

polysaccharidases, winemaking, polyphenols, malbec, grape cell wall

Citation

Related articles…

The use of rootstock as a lever in the face of climate change and dieback of vineyard

As viticulture faces challenges such as climate change or vineyard dieback, the choice of the variety and rootstock becomes more and more crucial. To study rootstock levers in the Bordeaux region, a parcel of Cabernet Sauvignon (CS) was planted with four rootstocks in 2014. Twenty repetitions of each of the following four rootstocks were set up: 101-14 MGt, Nemadex AB, 420A MGt and Gravesac. The number of bunches, yields and pruning weights of the vine shoots were measured individually on 240 vines from 2017 to 2021. Since 2020, nitrogen status assessed by assimilable nitrogen level, hydric status assessed by δ13C and berry maturity were measured on 80 samples taken from 20 repetitions of the four rootstocks. A lower yield was measured for CS grafted onto Nemadex AB due to the lower number of bunches and the lower weight of berries. The differences between the other three rootstocks are small, but CS grafted onto 420A MGt was the most productive. The CS grafted onto Nemadex AB had the lowest pruning weight while 101-14 MGt had the highest. In 2020, δ13C showed a more moderate water stress with 101-14 MGt and 420A MGt than with Nemadex AB. Surprisingly, the Gravesac was under more stress than the 101-14 MGt. The nitrogen status in the berries was better for Nemadex AB but this was perhaps due to the significantly lower weight of the berries.Rootstock 101-14 MGt attained the highest accumulation of sugars in the berries while 420A MGt allows to preserve higher acidity. The parcel is still young which may explain some of the results. These measures must therefore be continued over the next several years to fully assess the effects of these rootstocks on the development of the vines and the quality of the production under new climatic conditions.

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Soil quality in Beaujolais vineyard. Importance of pedology and cultural practices

A pedological study was carried out from 2009 to 2017 in Beaujolais vineyard, to improve physical and chemical knowledge of soils. It was completed in 2016 and 2017 by the current study, dealing with microbial aspects, in order to build a reference frame for improved advice in soil management. Microbial biomass was measured on representative plots of the six most common soil types identified in Beaujolais and, for each soil type, on plots with different levels of the main impacting parameters: total organic carbon, pH, cation exchange capacity, extractable copper. A total of 59 soil samples were collected. Confirming the results of various trials carried out in Beaujolais over the past 20 years, the results of the present study showed that the soils were still alive, but exhibited a large variability of biological parameters, which appeared dependant on both pedological and anthropic factors. Therefore, a good interpretation of biological parameters and advice for vine growers must rely on a pedologically-based referential with differentiated main driving factors. For example, the control of pH is of primary importance in granitic soils and in no way organic matter addition can improve soil quality if pH is too low. Conversely, in calcareous soils, biological parameters are more directly affected by direct or indirect (cover crops for example) inputs of organic matter. The use of biological parameters, such as microbial biomass, is of great potential value to improve advice on agro-viticultural practices (soil management, fertilization, liming, etc.), basis of a sustainable wine production on fragile soils.

Climate projections over France wine-growing region and its potential impact on phenology

Climate change represents a major challenge for the French wine industry. Climatic conditions in French vineyards have already changed and will continue to evolve. One of the notable effects on grapevine is the advancing growing season. The aim of this study is to characterise the evolution of agroclimatic indicators (Huglin index, number of hot days, mean temperature, cumulative rainfall and number of rainy days during the growing season) at French wine-growing regions scale between 1980 and 2019 using gridded data (8 km resolution, SAFRAN) and for the middle of the 21th century (2046-2065) with 21 GCMs statistically debiased and downscaled at 8 km. A set of three phenological models were used to simulate the budburst (BRIN, Smoothed-Utah), flowering, veraison and theoretical maturity (GFV and GSR) stages for two grape varieties (Chardonnay and Cabernet-Sauvignon) over the whole period studied. All the French wine-growing regions show an increase in both temperatures during the growing season and Huglin index. This increase is accompanied by an advance in the simulated flowering (+3 to +9 days), veraison (+6 to +13 days) and theoretical maturity (+6 to +16 days) stages, which are more noticeable in the north-eastern part of France. The climate projections unanimously show, for all the GCMs considered, a clear increase in the Huglin index (+662 to 771 °C.days compared to the 1980-1999 period) and in the number of hot days (+5.6 to 22.6 days) in all the wine regions studied. Regarding rainfall, the expected evolution remains very uncertain due to the heterogeneity of the climates simulated by the 21 models. Only 4 regions out of 21 have a significant decrease in the number of rainy days during the growing season. The two budburst models show a strong divergence in the evolution of this stage with an average difference of 18 days between the two models on all grapevine regions. The theoretical maturity is the most impacted stage with a potential advance between 40 and 23 days according to wine-growing regions.

A spatial explicit inventory of EU wine protected designation of origin to support decision making in a changing climate

Winemaking areas recognized as protected designations of origin (PDOs) shape important economic, environmental and cultural values that are tied to closely defined geographic locations. To preserve wine products and wine-growing practices adopted in different PDOs these areas are strictly regulated by legal specifications. However, quality viticulture is increasingly under pressure from climate change, which is altering the local conditions of many winegrowing areas. Therefore, maintaining traditional wine products will require the adoption of tailored adaptation strategies, including possible changes in the legal regulation of protected wines. To this end, it is necessary to have a comprehensive knowledge on PDOs including their extension, products and allowed practices. While there have been efforts to build databases that summarize the characteristics for individual wine PDO areas and to quantify the related effects of climate change, much information is still included only in the official documentation of the EU geographical indication register and has never been collected in a comprehensive manner. With this study we aim at filling this gap by building a spatial inventory of European wine PDOs that supports decision making in viticulture in the context of climate change. To map and characterize European wine PDOs, we analysed their legal documents and extracted relevant information useful for climate change adaptation. The output consists of a comprehensive geographical dataset that identifies the boundaries of all 1200 European wine PDOs at unprecedented spatial resolution and includes a set of legally binding regulations, such as authorized vine varieties, maximum yields and planting density. The inventory will allow researchers to analyse the impacts of climate change on European wine PDOs and support decision makers in developing tailored adaptation strategies. This includes, among others, the evaluation of new vineyard site selection, the expansion of cultivated varieties or the authorization of irrigation in vineyards.