Macrowine 2021
IVES 9 IVES Conference Series 9 Assay of distinct modes of polysaccharidases dosage in vinification with cv. Malbec. Effects on microbiological evolution, color and skin depletion

Assay of distinct modes of polysaccharidases dosage in vinification with cv. Malbec. Effects on microbiological evolution, color and skin depletion

Abstract

In the maceration stage of winemaking, enzymes can be used to degrade the polysaccharides present in the cell walls and middle sheets, and thus facilitate the extraction of juice and the release of polyphenols and aroma precursors retained in the grape skins. This work aims to analyze the influence of two enzyme complexes produced by autochthonous yeasts on the red winemaking process, in order to evaluate their effect on the chemical composition of the wines obtained, as well as on the extraction of color and polyphenols, and the depletion of the skin. Two strains previously selected for the effect of their enzymatic complex on the color extraction and improvement in the technological properties of the grape must were used (Longhi et al., 2019). A multi-enzymatic extract from Aureobasidium pullulans m11-2 was obtained by inoculating the microorganism in a broth according to Moyo et al. (2003) with modifications (pH 3.8) and incubated with stirring at 28°C for 72 h. Pectinase, xylanase, cellulase and amylase activities were quantified by determining reducing sugars by DNS, modified by Qian Li et al. (2015). Likewise, Torulaspora delbrueckii m7-2 was used for the production of the enzyme complex during vinification. Malbec red grapes (Vitis vinifera L.) from San Rafael (Mendoza) wine region, vintage 2021, were used to conduct the vinifications. The must obtained by crushing 60 Kg of grapes was corrected in acidity, sulfited (50 ppm) and distributed in 5 L containers. Four winemaking assays were performed, in duplicate: (1) inoculation with a native strain of Sacchromyces cerevisiae (SR1), at 108 cell/mL as inoculum, conducted at 20°C (control, C); (2) sequential inoculation of T. delbrueckii m7-2, with an initial cellular concentration of 107 cells/mL, followed by SR1 inoculation at 4th day (Td); (3) cold pre-fermentation maceration (CPM, 8°C-4 days) with m11-2 enzyme extract and SR1 inoculation (Ap); and (4) CPM without enzymatic treatment and SR1 inoculation (E). Growth kinetics of total yeasts were determined on YPD and DRBC agar, and of non-Saccharomyces yeasts in lysine medium. All enzymatic activities were monitored at pH 3.80 and 20°C. The pectinolytic activity was the main one, showing a level of 1.80 U/mL in the m11-2 extract and an initial level of 1.47 U/mL for the in situ producer strain (m7-2). Microscopic observations of the extracted skins in Ap and E vinifications were carried out to evaluate the effect of the enzymatic complex m11-2 on the cell wall, and were also compared with the fresh grape skins. Differences were observed between the skins enzymatically treated (Ap) and the control (E); the former showed cell emptying, greater rupture of the epidermis layers and less firmness, unlike the control that exhibited almost intact epidermal layers. These images allowed us to know the cell morphology of the varietal cv. Malbec and the enzymatic hydrolysis of its cell walls.

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Sara Jaquelina Longhi 

 Biotechnology Laboratory, Department of Biology and Food, Faculty of Sciences Applied to Industry, National University of Cuyo. Bernardo de Irigoyen 375, San Rafael, Mendoza, Argentina. National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Autonomous City of Buenos Aires, Argentina. ,María Carolina MARTÍN (1,2)  María Belén AVENDAÑO(1) María Gabriela MERÍN (1,2)   Luciana Paola PRENDES (1,2) Juliana GARAU (1,2) Vilma Inés MORATA DE AMBROSINI (1,2) (1) Biotechnology Laboratory, Department of Biology and Food, Faculty of Sciences Applied to Industry, National University of Cuyo. Bernardo de Irigoyen 375, San Rafael, Mendoza, Argentina. (2) National Council for Scientific and Technical Research (CONICET), Godoy Cruz 2290, Autonomous City of Buenos Aires, Argentina

Contact the author

Keywords

polysaccharidases, winemaking, polyphenols, malbec, grape cell wall

Citation

Related articles…

Assessing the climate change vulnerability of European winegrowing regions by combining exposure, sensitivity and adaptive capacity indicators

Winegrowing regions recognized as protected designations of origin (PDOs) are closely tied to well defined geographic locations with a specific set of pedoclimatic attributes and strictly regulated by legal specifications. However, climate change is increasingly threatening these regions by changing local conditions and altering winegrowing processes. The vulnerability to these changes is largely heterogenous across different winegrowing regions because it is determined by individual characteristics of each region, including the capacity to adapt to new climatic conditions and the sensitivity to climate change, which depend not only on natural, but also socioeconomic and legal factors. Accurate vulnerability assessments therefore need to combine information about adaptive capacity and climate change sensitivity with projected exposure to new climatic conditions. However, most existing studies focus on specific impacts neglecting important interactions between the different factors that determine climate change vulnerability. Here, we present the first comprehensive vulnerability assessment of European wine PDOs that spatially combines multiple indicators of adaptive capacity and climate change sensitivity with high-resolution climate projections. We found that the climate change vulnerability of PDO areas largely depends on the complex interactions between physical and socioeconomic factors. Homogenous topographic conditions and a narrow varietal spectrum increase climate change vulnerability, while the skills and education of farmers, together with a good economic situation, decrease their vulnerability. Assessments of climate change consequences therefore need to consider multiple variables as well as their interrelations to provide a comprehensive understanding of the expected impacts of climate change on European PDOs. Our results provide the first vulnerability assessment for European winegrowing regions at high spatiotemporal resolution that includes multiple factors related to climate exposure, sensitivity, and adaptive capacity on the level of single winegrowing regions. They will therefore help to identify hot spots of climate change vulnerability among European PDOs and efficiently direct adaptation strategies.

Climate ethnography and wine environmental futures

Globalisation and climate change have radically transformed world wine production upsetting the established order of wine ecologies. Ecological risks and the future of traditional agricultural systems are widely debated in anthropology, but very little is understood of the particular challenges posed by climate change to viticulture which is seen by many as the canary in the coalmine of global agriculture. Moreover, wine as a globalised embedded commodity provides a particularly telling example for the study of climate change having already attracted early scientific attention. Studies of climate change in viticulture have focused primarily on the production of systematic models of adaptation and vulnerability, while the human and cultural factors, which are key to adaptation and sustainable futures, are largely missing. Climate experts have been unanimous in recognising the urgent need for a better understanding of the complex dynamics that shape how climate change is experienced and responded to by human systems. Yet this call has not yet been addressed. Climate ethnography, coined by the anthropologist Susan Crate (2011), aims to bridge this growing disjuncture between climate science and everyday life through the exploration of the social meaning of climate change. It seeks to investigate the confrontation of its social salience in different locations and under different environmental guises (Goodman 2018: 340). By understanding how wine producers make sense of the world (and the environment) and act in it, it proposes to focus on the co-production of interdisciplinary knowledge by identifying and foreshadowing problems (Goodman 2018: 342; Goodman & Marshall 2018). It seeks to offer an original, transformative and contrasted perspective to climate change scenarios by investigating human agency -individual or collective- in all its social, political and cultural diversity. An anthropological approach founded on detailed ethnographies of wine production is ideally placed to address economic, social and cultural disruptions caused by the emergence of these new environmental challenges. Indeed, the community of experts in environmental change have recently called for research that will encompass the human dimension and for more broad-based, integrated through interdisciplinarity, useful knowledge (Castree & al 2014). My paper seeks to engage with climate ethnography and discuss what it brings to the study of wine environmental futures while exploring the limitations of the anthropological environmental approach.

Modulation of berry composition by different vineyard management practices

High concentration of sugars in grapes and alcohol in wines is one of the consequences of climate change on viticulture production in several wine-growing regions. In order to investigate the possibilities of adaptation of vineyard management practices aimed to reduce the accumulation of sugar during the maturation phase without reducing the accumulation of anthocyanins in grapes, a study with severe shoot trimming, shoot thinning, cluster thinning and date of harvest was conducted on Merlot variety in Istria region (Croatia), under the Mediterranean climate. Four factors which may affect grape maturation and its composition at harvest were investigated in a two-years experiment; severe shoot trimming applied at veraison when >80% of berries changed colour (in comparison to untreated control), shoot thinning (0 and 30%), cluster thinning (0 and 30%), and the date of harvest (early and standard harvest dates). Shoot thinning had no significant impact on berry composition, despite the obtained reduction in yield per vine. Lower Brix in grapes were obtained with earlier harvest date and if no cluster thinning was applied, although at the same time a reduction in the concentration of anthocyanins in berries was observed in these treatments. On the other hand, if severe shoot trimming was applied when >80% of berries changed colour, a reduction of Brix was obtained without a negative impact on berry anthocyanins concentration. We conclude that in cases when undesirably high sugar concentrations at harvest are expected, severe shoot trimming at 80% veraison may effectively be used in order to obtain moderate sugar concentration in berries together with the adequate phenolic composition.

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.