Macrowine 2021
IVES 9 IVES Conference Series 9 Evolution of flavonols during Merlot winemaking processes

Evolution of flavonols during Merlot winemaking processes

Abstract

Aim: The phenomenon of quercetin precipitation in wine (flanovol haze), has been manifested for many years in several wine-producing regions, such as Italy, Australia, and New Zealand (Gambuti et al., 2020; Lanati, Marchi, & Cascio, 2014; Somers & Ziemelis, 1985). Due to the limited information related to the quercetin aglycone behavior and its precursors during wine production in New Zealand, this study aims to monitor the evolution of flavonols and other polyphenols during the commercial fermentation of Merlot grapes, using different fermentation conditions, and vineyard treatments.

Methods: Various trials evaluating sun exposure, winemaking practices, and winemaking process management were undertaken using Merlot grapes, commercial yeast cultures, potassium metabisulphite (20 g/hL), and nutrient supplementation with DYNASTART®-LAFFORT at 20 g/hL. Samples were taken through the winemaking stages, and the polyphenols were quantified using a reversed-phase HPLC method (Garrido-Bañuelos et al., 2019; Peng et al. 2002).

Results: Grapes with elevated amounts of flavonols glycosides produced wines with higher levels of flavonol glycosides and quercetin. Wines made from grapes with greater sun exposure ended up with more flavonol glycosides (89 mg/L) and quercetin (16 mg/L) than the wines elaborated from less exposed grapes (47 mg/L and 9.4 mg/L, respectively). Certain winemaking practices showed differences in quercetin content, for example using small fermentation (250 kg) (12 mg/L), and large fermentation (five tonnes) (28 mg/L). The data also indicates that tannins and total anthocyanins were present at 786 mg/L and 156 mg/L, respectively, for small-scale ferments, and at 888 mg/L and 363 mg/L, respectively, for large-scale ferments. In evaluating the winemaking process management, the ferment pumped over (largest fermentation volume) exhibited flavonol glycosides and quercetin at the highest concentration (91 mg/L and 20 mg/L, respectively), compared to the remaining treatments. PMS, enzyme, and PMS plus enzyme, additions lowered the concentration of the flavonols glycosides at the end of the winemaking process (37 mg/L, 42 mg/L, and 43 mg/L, respectively). It was seem that the PMS plus enzyme (15.6 mg/L) increase quecetin in wine when compared to the control, no additions, (12.6 mg/L). The wines treated with enzyme, PMS, and PMS plus enzyme, also had lower concentrations of anthocyanins (215 mg/L, 233 mg/L, and 238 mg/L, respectively) than the control (291 mg/L). 

Conclusions

The study confirmed past research on the role of sun exposure in the formation of flavonols in Merlot grapes and wines. Fermentation size can improve the extraction of polyphenols into wine, and the enzyme additions can promote the hydrolysis of flavonol glycosides. In considering winemaking practices to lower flavonol content, the impact on remaining wine phenolics, of importance to wine colour and mouthfeel, also needs to be carefully evaluated.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Cristian Hernandez

School of Chemistry University of Auckland,Paul KILMARTIN, School of Chemistry, University of Auckland Leandro DIAS, School of Food Science, University of Auckland Gianni FLEGO, Villa Maria Estate winery Rebecca DEED, School of Biological Sciences, University of Auckland

Contact the author

Citation

Related articles…

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.

Grapevine sugar concentration model in the Douro Superior, Portugal

Increasingly warm and dry climate conditions are challenging the viticulture and winemaking sector. Digital technologies and crop modelling bear the promise to provide practical answers to those challenges. As viticultural activities strongly depend on harvest date, its early prediction is particularly important, since the success of winemaking practices largely depends upon this key event, which should be based on an accurate and advanced plan of the annual cycle. Herein, we demonstrate the creation of modelling tools to assess grape ripeness, through sugar concentration monitoring. The study area, the Portuguese Côa valley wine region, represents an important terroir in the “Douro Superior” subregion. Two varieties (cv. Touriga Nacional and Touriga Franca) grown in five locations across the Côa Region were considered. Sugar accumulation in grapes, with concentrations between 170 and 230 g l-1, was used from 2014 to 2020 as an indicator of technological maturity conditioned by meteorological factors. The climatic time series were retrieved from the EU Copernicus Service, while sugar data were collected by a non-profit organization, ADVID, and by Sogrape, a leading wine company. The software for calibrating and validating this model framework was the Phenology Modeling Platform (PMP), version 5.5, using Sigmoid and growing degree-day (GDD) models for predictions. The performance was assessed through two metrics: Roots Mean Square Error (RMSE) and efficiency coefficient (EFF), while validation was undertaken using leave-one-out cross-validation. Our findings demonstrate that sugar content is mainly dependent on temperature and air humidity. The models achieved a performance of 0.65

Modulation of berry composition by different vineyard management practices

High concentration of sugars in grapes and alcohol in wines is one of the consequences of climate change on viticulture production in several wine-growing regions. In order to investigate the possibilities of adaptation of vineyard management practices aimed to reduce the accumulation of sugar during the maturation phase without reducing the accumulation of anthocyanins in grapes, a study with severe shoot trimming, shoot thinning, cluster thinning and date of harvest was conducted on Merlot variety in Istria region (Croatia), under the Mediterranean climate. Four factors which may affect grape maturation and its composition at harvest were investigated in a two-years experiment; severe shoot trimming applied at veraison when >80% of berries changed colour (in comparison to untreated control), shoot thinning (0 and 30%), cluster thinning (0 and 30%), and the date of harvest (early and standard harvest dates). Shoot thinning had no significant impact on berry composition, despite the obtained reduction in yield per vine. Lower Brix in grapes were obtained with earlier harvest date and if no cluster thinning was applied, although at the same time a reduction in the concentration of anthocyanins in berries was observed in these treatments. On the other hand, if severe shoot trimming was applied when >80% of berries changed colour, a reduction of Brix was obtained without a negative impact on berry anthocyanins concentration. We conclude that in cases when undesirably high sugar concentrations at harvest are expected, severe shoot trimming at 80% veraison may effectively be used in order to obtain moderate sugar concentration in berries together with the adequate phenolic composition.

Protected Designation of Origin (D.P.O.) Valdepeñas: classification and map of soils

The objective of the work described here is the elaboration of a map of the different types of vineyard soils that to guide the famers in the choice of the most productive vine rootstocks and varieties. 90 vineyard soils profiles were analysed in the entire territory of the Origen Denominations of Valdepeñas. The sampling was carried out in 2018 (June to October) by making a sampling grid, followed by photointerpretation and control in the field. The studied soils can be grouped into 9 different soil types (according to FAO 2006 classification): Leptosols, Regosols, Fluvisols, Gleysols, Cambisols, Calcisols, Luvisols and Anthrosols. A map showing the soil distribution with different type of soils has been made with the ArcGIS program. Regarding to the choice of rootstock, Calcisoles are soils with a high active limestone content, so the rootstocks used in these soils must be resistant to this parameter; Luvisols are deep soils with high clay content, so they will support vigorous rootstocks. Because the cartographic units are composed of two or more subgroups, with are associated in variable proportions, 9 different soil associations have been established; Unit 1: Leptosols, Cambisols and Luvisols (80%, 15% and 5% respectively); Unit 2: Cambisols with Regosols and Luvisols (40%, 30% and 30% respectively); Unit 3: Cambisols and Gleysols with Regosols (40%, 40% and 20% respectively); Unit 4: Regosols with Cambisols, Leptosols and Calcisols (40%, 30%, 15% and 15% respectively); Unit 5: Cambisols, Leptosols, Calcisols and Regosols (25% each of them); Unit 6: Luvisols with Cambisol and Calcisols (80%, 10% and 10% respectively); Unit 7: Luvisols and Calcisols with Cambisols (40%, 40% and 20% respectively); Unit 8: Calcisols with, Cambisols and Luvisols (80%, 10% and 10% respectively); Unit 9: Anthrosols. These study allow to elaborate the first map of vineyard soils of this Protected Designation of Origin in Castilla-La Mancha.