Macrowine 2021
IVES 9 IVES Conference Series 9 Evolution of flavonols during Merlot winemaking processes

Evolution of flavonols during Merlot winemaking processes

Abstract

Aim: The phenomenon of quercetin precipitation in wine (flanovol haze), has been manifested for many years in several wine-producing regions, such as Italy, Australia, and New Zealand (Gambuti et al., 2020; Lanati, Marchi, & Cascio, 2014; Somers & Ziemelis, 1985). Due to the limited information related to the quercetin aglycone behavior and its precursors during wine production in New Zealand, this study aims to monitor the evolution of flavonols and other polyphenols during the commercial fermentation of Merlot grapes, using different fermentation conditions, and vineyard treatments.

Methods: Various trials evaluating sun exposure, winemaking practices, and winemaking process management were undertaken using Merlot grapes, commercial yeast cultures, potassium metabisulphite (20 g/hL), and nutrient supplementation with DYNASTART®-LAFFORT at 20 g/hL. Samples were taken through the winemaking stages, and the polyphenols were quantified using a reversed-phase HPLC method (Garrido-Bañuelos et al., 2019; Peng et al. 2002).

Results: Grapes with elevated amounts of flavonols glycosides produced wines with higher levels of flavonol glycosides and quercetin. Wines made from grapes with greater sun exposure ended up with more flavonol glycosides (89 mg/L) and quercetin (16 mg/L) than the wines elaborated from less exposed grapes (47 mg/L and 9.4 mg/L, respectively). Certain winemaking practices showed differences in quercetin content, for example using small fermentation (250 kg) (12 mg/L), and large fermentation (five tonnes) (28 mg/L). The data also indicates that tannins and total anthocyanins were present at 786 mg/L and 156 mg/L, respectively, for small-scale ferments, and at 888 mg/L and 363 mg/L, respectively, for large-scale ferments. In evaluating the winemaking process management, the ferment pumped over (largest fermentation volume) exhibited flavonol glycosides and quercetin at the highest concentration (91 mg/L and 20 mg/L, respectively), compared to the remaining treatments. PMS, enzyme, and PMS plus enzyme, additions lowered the concentration of the flavonols glycosides at the end of the winemaking process (37 mg/L, 42 mg/L, and 43 mg/L, respectively). It was seem that the PMS plus enzyme (15.6 mg/L) increase quecetin in wine when compared to the control, no additions, (12.6 mg/L). The wines treated with enzyme, PMS, and PMS plus enzyme, also had lower concentrations of anthocyanins (215 mg/L, 233 mg/L, and 238 mg/L, respectively) than the control (291 mg/L). 

Conclusions

The study confirmed past research on the role of sun exposure in the formation of flavonols in Merlot grapes and wines. Fermentation size can improve the extraction of polyphenols into wine, and the enzyme additions can promote the hydrolysis of flavonol glycosides. In considering winemaking practices to lower flavonol content, the impact on remaining wine phenolics, of importance to wine colour and mouthfeel, also needs to be carefully evaluated.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Cristian Hernandez

School of Chemistry University of Auckland,Paul KILMARTIN, School of Chemistry, University of Auckland Leandro DIAS, School of Food Science, University of Auckland Gianni FLEGO, Villa Maria Estate winery Rebecca DEED, School of Biological Sciences, University of Auckland

Contact the author

Citation

Related articles…

Terroir traceability in grapes, musts and wine: results of research on Gewürztraminer and Sauvignon Blanc grape varieties in northern Italy

In the study of terroir, a separate analysis of its many component factors can be of great help in accurately identifying a vineyard’s natural elements that impact wine quality and typicity. This research used a dedicated pluri-disciplinary approach to investigate the ecological characteristics, including geology and geographical features, of 14 vineyards that produce Gewürztraminer and Sauvignon Blanc cultivars in the alpine Alto Adige DOC wine region. Both the geopedological method using Vineyards Geological Identity (VGI) and the new Solar Radiaton Identity (SRI) topoclimatic classification method were used to provide analytical measurements and qualitative/quantitative characterisations. In addition, wide-ranging targeted and untargeted oenological and chemical analyses were carried out on grapes, musts and wines to correlate the soils’ geomineral and physical conditions with the biochemical properties of their fruits and wines. The research identified strong correlations between vineyard geo-identity and wine biofingerprint, confirming a mineral traceability of strontium rubidium ratio and some minerals distinctive to the local geology, such as K, Ca, Ag, Ba and Mn.  The study also discovered that particular geomineral and physical soil conditions of the studied vineyards are related to the different amount of amino acids, primary varietal aromas and polyphenols found in grapes, musts and wines. The research confirmed that winemaking technologies support oenological quality, although in some cases, human practices can overpower certain characteristic elements in wine, erasing the typical imprint left by the vineyards’ natural terroir, which becomes less traceable. Terroir abiotic ecological factors and vineyard identity can be classified in detail using the new VGI and SRI analysis methods to discover interrelationships between geo-pedological and topoclimatic conditions that impact wine quality. These methods are also helpful in identifying which ecological elements are exclusive to a particular vineyard or wine sub-region.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

Climate change impacts on Douro Region viticulture and adaptation measures

Climate has a significant impact in the success of any agricultural system, with a direct influence on the crops suitability to a given region, interfering on yield and quality and also with the economic sustainability of the productive activity. In the Douro Demarcated Region (RDD), as in most regions of the Mediterranean climate, the scarce precipitation (33% has less than 600 mm per year), and your high variability, associated with high rates of evapotranspiration during the summer, is usually one of the fundamental factors that limit the grapevine development, as well as the production and quality of the harvest. Thus, facing the scenario in temperature changes for the next decades (1.5-2.5°C) and confirming the predictions of precipitation decreases and/or great variability in the occurrence of heat waves and intense rainfall, the consequences for slope stability in mountain viticulture and sustainability of all operations involved, are risks to be taken into account. In this way, a deepest and sustained knowledge regarding the adaptation measures to adverse environmental conditions is of a crucial importance, enabling a more efficient adaptation of plant growth conditions and the optimization of production and quality of the grapevines. The development of this work, carried out in two commercial vineyards, one located in Soutelo do Douro, São João da Pesqueira, Cima Corgo sub-region, and another located in Numão, Vila Nova de Foz Côa, Douro Superior sub-region, it seeks to establish a relationship between climatic elements and physiological, productive and qualitative parameters, as well as to evaluate the effectiveness of adaptation measures, including different types of deficit irrigation (2002-2019) and the application of shading nets (2019-2020) in the physiological, viticultural and oenological behavior in the Touriga Nacional and Moscatel Galego Branco varieties, respectively. The results showed that the application of deficit irrigation allowed to significantly reduce the impact of the adverse weather conditions at key moments in the development of the grapevine, particularly in the period immediately before veráison and maturation, reducing the negative effects on the physiological processes and productivity, without compromise the must quality parameters. On the other hand, the application of shading nets significantly reduced de leaves temperature, allowing to increase the water potential, stomatal conductance and photosynthetic rate of grapes, which was reflected in the yield increase in the 2nd year of the study. For the maturation indicators, higher levels of total acidity, malic acid and assimilable nitrogen were obtained. The last measure presents a huge potential, being essential to carry out more years of trials to obtain stronger conclusions in terms of production parameters, but also in characteristics as important as the grape ripening components and the organoleptic characteristics of wines.

Assessing the climate change vulnerability of European winegrowing regions by combining exposure, sensitivity and adaptive capacity indicators

Winegrowing regions recognized as protected designations of origin (PDOs) are closely tied to well defined geographic locations with a specific set of pedoclimatic attributes and strictly regulated by legal specifications. However, climate change is increasingly threatening these regions by changing local conditions and altering winegrowing processes. The vulnerability to these changes is largely heterogenous across different winegrowing regions because it is determined by individual characteristics of each region, including the capacity to adapt to new climatic conditions and the sensitivity to climate change, which depend not only on natural, but also socioeconomic and legal factors. Accurate vulnerability assessments therefore need to combine information about adaptive capacity and climate change sensitivity with projected exposure to new climatic conditions. However, most existing studies focus on specific impacts neglecting important interactions between the different factors that determine climate change vulnerability. Here, we present the first comprehensive vulnerability assessment of European wine PDOs that spatially combines multiple indicators of adaptive capacity and climate change sensitivity with high-resolution climate projections. We found that the climate change vulnerability of PDO areas largely depends on the complex interactions between physical and socioeconomic factors. Homogenous topographic conditions and a narrow varietal spectrum increase climate change vulnerability, while the skills and education of farmers, together with a good economic situation, decrease their vulnerability. Assessments of climate change consequences therefore need to consider multiple variables as well as their interrelations to provide a comprehensive understanding of the expected impacts of climate change on European PDOs. Our results provide the first vulnerability assessment for European winegrowing regions at high spatiotemporal resolution that includes multiple factors related to climate exposure, sensitivity, and adaptive capacity on the level of single winegrowing regions. They will therefore help to identify hot spots of climate change vulnerability among European PDOs and efficiently direct adaptation strategies.