Influence of grape withering on corvina and corvinone aroma composition

Cette recherche a eu pour but l’étude des effets d’un renforcement radiatif et thermique sur les zones inférieures de la canopée de la vigne (solarisation par des films ou des paillages réfléchissants installés sur le sol, sous les ceps), notamment l’étude de leurs conséquences sur la composition biochimique des moûts à la vendange et sur la qualité des vins.

Assessing grape composition is critical in vineyard management. It is required to decide the harvest date and to optimize cultural practices toward the achievement of production goals. The grape composition is variable in time and space, as it is affected by the ripening process and depends on soil and climate conditions.

Vine diseases have a strong impact on vineyards sustainability, which in turns leads to strong economic consequences. Among those diseases, Flavescence dorée spreads quickly and is incurable, which led in France to the setup of a mandatory pest control implying the systematic use of pesticides and the prospection and uprooting of every infected plants. Remote sensing could be a very powerful tool to optimize prospection as it allows to produce quickly accurate maps over large areas. Recent studies have shown that high spatial resolution (10cm/pixel) multispectral images acquired from UAVs allow to map Flavescence dorée in vineyards using leaves discolorations [e.g. Albetis et al., Remote Sensing, 2017].

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.

Climate change is increasing the frequency of water deficit in many grape-growing regions. Grapevine varieties differ in their stomatal behavior during water deficit, and their ability to regulate water potential under dry soil conditions is commonly differentiated using the concept of isohydricity. It remains unclear whether stomatal behavior, water potential regulation, and the resulting degree of isohydricity has a relationship with changes to fruit growth during water deficit. This study was conducted on four varieties (`Cabernet Franc`, `Semillon`, `Grenache`, and `Riesling`) subjected to both short-term, severe water deficit and long-term, moderate water deficit applied at both pre- and post-veraison.