Determination of target compounds in cava quality using liquid chromatography. Application of chemometric tools in data analysis

Agriculture plantations are complex systems whose performance critically depends on the execution of several types of tasks with precise timing and efficiency to respond to different external factors. This is particularly true for orchards like vineyards, which need to be strictly monitored and regulated, as they are sensitive to diverse types of pests, and climate conditions. In these environments, managing and optimally scheduling the available work force and resources is not trivial and is usually done by teams of senior managers based on their experience. In this regard, having a baseline schedule could help them in the decision process and improve their results, in terms of time and resources spent.

Context and purpose of the study. The growing need for sustainable solutions in viticulture has led to increased interest in biostimulants that can enhance plant resilience to both abiotic and biotic stresses.

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition).

The Matrix Assisted Laser Desorption/Ionization–Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology is commonly used in food and medical sector to identify yeast or bacteria species isolated from a nutritive culture media. Since a decade, brewery and oenology industries have been attracted to this method which combines fast analysis times, reliability and low cost of analysis. Briefly, this method is based on the comparison of the MALDI-TOF/MS protein spectra of an isolated colony of yeast or bacteria with those contain in a manufacturer’s reference protein spectra database. Initiated in 2015, the creation of the first oenological mass spectra database has proved to be essential for increase quality of species identification.

In the French AOCs, the production area of ​​the raw material can be subject to plot delimitation based on criteria of physical environment and use. On the other hand, many environmental zonings are developing and the AOCs are called upon include provisions relating to these concerns. Hedges, through their effects on local changes in the regional climate and on functional biodiversity, can impact the functioning of vines and orchards. It is for this reason that their consideration as a delimitation criterion is envisaged.