A mechanistic investigation of H/D scrambling processes in flavonoids

This study was carried out on 24 vintages of Cabernet sauvignon and on 7 vintages of Merlot produced by two different Bordeaux growing areas. Proanthocyanidin monomers and oligomers, and several parameters of the proanthocyanidin fraction were analytically assessed.

Muscat of Alexandria is one of the oldest cultivars still existing, globally recognized for its distinctive aroma, and the primary grape variety cultivated in the Greek Island of Lemnos, yielding various white wines with designated origins.

This study focuses on investigating the effects of climate change on the plant physiology and berries of Vitis vinifera cv “Monastrell” in a commercial vineyard managed organically in Southeastern Spain (Jumilla, Murcia). For this purpose, open top chambers and rainout shelters were employed to simulate warming (~2-7 ºC, W) and rainfall reduction (~30%, RR) respectively. Additionally, a combination of both treatments (W+RR) was employed. Vines without either top chambers or rainout shelters were considered as control (C). The experiment was established in February of 2023. Predawn leaf water potential (measured using a pressure chamber), stomatal conductance (assessed with a porometer at mid-morning) and leaf chlorophyll and flavonoid content (measured using the Dualex® leaf clip sensor) were analyzed at veraison (5 months after the installation of structures).

Winemakers are increasingly experimenting with new techniques, such as spontaneous fermentation, prolonged yeast contact, higher pH, minimal sulphur dioxid, filtration and clarification or oxidative ageing. Along with this, the risk of microbial spoilage increases, and so the off-flavour mousiness, long time underestimated, is becoming more frequent. Characteristic of the mousy off-flavour is the delayed perception after swallowing the wine. After a few seconds the flavour appears, reminiscent of a dirty mouse cage. There are three known compounds that cause mousy off-flavor: 2-ethyltetrahydropyridine, 2-acetyltetrahydopyridine, and 2-acetylpyrroline. Yeasts such as Dekkera/Brettanomyces and heterofermentative lactic acid bacteria like Lactobacillus hilgardii can release these compounds.

In greenhouse, emission of volatile organic compounds (VOC) by grapevine leaves has already been reported in response to the defence elicitor sulfated laminarin (PS3) [1]. In order to check that this response was not specific to PS3, experiments were conducted on Vitis cv Marselan plantlets with several other elicitors of different chemical structures: i.e. Bastid® (COS-OGA),