On sample preparation methods for fermentative beverage VOCs profiling by GCxGC-TOFMS

Abstract

AIM: Study the influence of sample preparation methods on the volatile organic compounds (VOCs) profiling for fermentative beverages by GCxGC-TOFMS analysis.

METHODS: Five common sample preparation methods were tested on pooled red wine, white wine, cider, and beer. Studied methods were DHS, Liquid-liquid extraction, mSBSE, SPE and SPME. VOCs were analyzed by GCxGC-TOFMS followed by data analysis with ChromaTOF. RESULTS: The volatile organic compounds (VOCs) profiling results were very dependent on the sample preparation methods. Consider the number of annotated VOCs: SPE sample preparation is most suitable for beer and red wine; 166 and 433 peaks were annotated respectively. For cider and white wine, most peaks were found by DHS (330) and L-L extraction (256). However, there is only a small fraction of VOCs can be found with all the sample preparation techniques. For known fermentative aromas, most of them can be found easily by all the sample preparation methods. SPME, compare to L-L extraction, mSBSE, and SPE, have a shortage of collection and concentration on lactone compounds and vinyl compounds.

CONCLUSIONS:

VOCs profiling results for the fermentative beverages vary based on the used sample preparation method. There isn’t one ideal method to collect and concentrate all the compounds. A good global coverage can be reached by combining the results from different sample preparation techniques.

DOI:

Publication date: September 28, 2021

Issue: Macrowine 2021

Type: Article

Authors

Penghan Zhang , Silvia CARLIN, Fulvio MATTIVI, Urska VRHOVSEK,

Edmund Mach Foundation

Contact the author

Citation

Related articles…

LARGE-SCALE PHENOTYPIC SCREENING OF THE SPOILAGE YEAST BRETTANOMYCES BRUXELLENSIS: UNTANGLING PATTERNS OF ADAPTATION AND SELECTION, AND CONSEQUENCES FOR INNOVATIVE WINE TREATMENTS

Brettanomyces bruxellensis is considered as the main spoilage yeast in oenology. Its presence in red wine leads to off-flavour due to the production of volatile phenols such as 4-vinylphenol, 4-vinylguaiacol, 4-ethylphenol and 4-ethylguaiacol, whose aromatic notes are unpleasant (e.g. animal, leather, horse or pharmaceutical). Beside wine, B. bruxellensis is commonly isolated from beer, kombucha and bioethanol production, where its role can be described as negative or positive. Recent genomic studies unveiled the existence of various populations.

Grapevine sensitivity to fungal diseases: use of a combination of terroir cartography and parcel survey

In front of the economic interest and seeking to respect their environment, the wine growers move gradually towards a policy of reasoning their plant health protection. This is why, starting from epidemiologic studies on grapevine pathogens, forecasting models of the risks are developed by research and experimentation bodies.

Terroir aspects in development of quality of Egri bikavér

Egri Bikavér (Bull’s Blood) is one of the most remarkable Hungarian red wines on inland and foreign markets as well. From the end of the 70’s the quality of Egri Bikavér was decreasing continually due to mass production. The concept of production of quality wines became general in the mid 90’s again and it resulted in a new Origin Control System, for the first time that of Egri Bikavér in Hungary.

Comparing vineyard irrigation management based in two different approaches: vegetation indices and SIMDualKc model

Water scarcity, high air temperatures, high vapor pressure deficit, and increasing frequency and intensity of extreme climatic events, namely heat waves, exert huge pressure on viticulture, as is the case of Mediterranean climates. Therefore, farmers rely more and more on irrigation to overcome these constraints. Deficit irrigation is a proved strategy to optimize irrigation efficiency and wine quality. The present study intends to demonstrate the application of precision techniques, namely remote sensing derived vegetation indices (VI) and an open source software, SIMDualKc, to compute crop evapotranspiration using the dual crop coefficient approach (Kcb + Ke), for deficit irrigation management.

“Garrigues”, part of the mediterranean vine terroirs

Les paysages viticoles méditerranéens présentent une originalité qui ne se retrouve nulle part ailleurs : ils associent des garrigues très odoriférantes à des parcelles de vignes souvent qualitatives. La connaissance empirique des vins du Languedoc par leurs dégustateurs a conduit la Chambre d’Agriculture de l’Hérault à supposer que les arômes de la garrigue environnante peuvent se retrouver dans les vins (arômes de ciste, de genévrier, … )