On sample preparation methods for fermentative beverage VOCs profiling by GCxGC-TOFMS

During 1982 started our wine growing project at the Puntalarga Hill, between 2500 and 2600 meters a.s.l.: 5.78 ºN, 72.98 ºW. Pinot noir, white Riesling and Riesling x Silvaner crossings are the most planted grapevines. Since 1984 research and development activities are carried out on pertinent subjects.

The available plant diversity is maintained in global genetic collections and germplasm banks. One of the main objectives of the study of the genetic material of vine still conducting research to characterize the genotypes and the creation of new varieties. The main ampelographic collection of the country, the largest in the Balkans, is located at the Athens Vine Institute in Lykovrisi, Attica, in an area of 70 acres. It contains more than 800 varieties, most of which are indigenous. The Institute is conducting research on the genetic improvement of native varieties and the creation new winemaking and table grape varieties of high productivity, grape quality, resistance to fungal diseases and their adaptability to stresses using the hybridization method using European high-quality varieties.

Brettanomyces bruxellensis is considered as the main spoilage yeast in oenology. Its presence in red wine leads to off-flavour due to the production of volatile phenols such as 4-vinylphenol, 4-vinylguaiacol, 4-ethylphenol and 4-ethylguaiacol, whose aromatic notes are unpleasant (e.g. animal, leather, horse or pharmaceutical). Beside wine, B. bruxellensis is commonly isolated from beer, kombucha and bioethanol production, where its role can be described as negative or positive. Recent genomic studies unveiled the existence of various populations.

Context and purpose of the study. In regions where grapevines are grown under irrigation, like most regions in Argentina, the wine industry should adopt more sustainable strategies and production systems towards a higher water use efficiency and a reduction in no-renewable energy consumption.

The sustainability certification ‘nachhaltig austria’ (www.sustainableaustria.com) has been offered to austrian wineries in an online version for 10 years and over 25% of the austrian wine-growing area is now certified. Since the 2022 harvest, ‘nachhaltig austria’ has automatically calculated the carbon footprint for each winery, per hectare of vineyard, per litre of bulk wine and per 0.75-litre bottle (poelz, w. And rosner, f.g. 2023). In last year’s publications and numerous presentations at national and international level, topics such as refilling glass bottles, lightweight glass bottles, renewable energy, … Etc.