On sample preparation methods for fermentative beverage VOCs profiling by GCxGC-TOFMS

Abstract

AIM: Study the influence of sample preparation methods on the volatile organic compounds (VOCs) profiling for fermentative beverages by GCxGC-TOFMS analysis.

METHODS: Five common sample preparation methods were tested on pooled red wine, white wine, cider, and beer. Studied methods were DHS, Liquid-liquid extraction, mSBSE, SPE and SPME. VOCs were analyzed by GCxGC-TOFMS followed by data analysis with ChromaTOF. RESULTS: The volatile organic compounds (VOCs) profiling results were very dependent on the sample preparation methods. Consider the number of annotated VOCs: SPE sample preparation is most suitable for beer and red wine; 166 and 433 peaks were annotated respectively. For cider and white wine, most peaks were found by DHS (330) and L-L extraction (256). However, there is only a small fraction of VOCs can be found with all the sample preparation techniques. For known fermentative aromas, most of them can be found easily by all the sample preparation methods. SPME, compare to L-L extraction, mSBSE, and SPE, have a shortage of collection and concentration on lactone compounds and vinyl compounds.

CONCLUSIONS:

VOCs profiling results for the fermentative beverages vary based on the used sample preparation method. There isn’t one ideal method to collect and concentrate all the compounds. A good global coverage can be reached by combining the results from different sample preparation techniques.

DOI:

Publication date: September 28, 2021

Issue: Macrowine 2021

Type: Article

Authors

Penghan Zhang , Silvia CARLIN, Fulvio MATTIVI, Urska VRHOVSEK,

Edmund Mach Foundation

Contact the author

Citation

Related articles…

Litchi tomato as a fumigation alternative in Washington state wine grape vineyards

The northern root-knot nematode (Meloidogyne hapla) is one of the most prevalent plant-parasitic nematodes affecting Washington State Vitis vinifera vineyards. This nematode induces small galls on roots, restricting water and nutrient uptake. In new vineyards this can impede establishment. In existing vineyards, it can exacerbate decline in chronically stressed vines. While preplant fumigation is a common strategy for M. hapla management, its efficacy is temporary and relies on broad-spectrum chemicals that undergo frequent regulatory scrutiny. The trap crop litchi tomato (Solanum sisymbriifolium) showed promise in reducing plant-parasitic nematode densities in potato. This prompted field greenhouse experiments to evaluate its potential to reduce M. hapla in V. vinifera.

Évaluation environnementale de pratiques vitivinicoles innovantes

The Institut Français De La Vigne Et Du Vin (IFV) is conducting many experiments on innovative winegrowing practices, which are emerging in companies in the sector, or which are still at the R&D stage for agricultural suppliers. The purpose of these practices may be to reduce environmental impact, to adapt vineyards to climate change, or to achieve other technical, economic or social aims. Whatever the objective, it is necessary to verify the relevance of these new practices, and in particular their environmental relevance, i.e. That at the very least, the changes in practices do not increase the environmental impact of the technical itineraries.

A new approach for sensory characterization of grape. Relationship with chemical composition

Characterize taste and mouthfeel properties of grapes elicited by the phenolic fraction (PF) of grape berries and establish relationships with chemical variables. METHODS: As many as 31 diverse grape lots of Tempranillo Tinto and Garnacha Tinta from three different regions were harvested. Grapes were destemmed and macerated in 15% of ethanol for one week and extracts were submitted to solid phase extraction. The recovered polyphenolic fraction was reconstituted in wine model and characterized by a panel of 21 wine experts employing a list of 23 taste and mouthfeel-related attributes following a rate-k-attributes methodology. RESULTS: Six significant attributes among the 31 samples differed based on ANOVA results: “dry”, “coarse”, “bitter”, “dry on tongue”, “sticky” and “watery”. PCA with VARIMAX algorithm was calculated.

New plant protein extracts as fining agents for red wines

AIM: Quinoa (Chenopodium quinoa) is a non-allergenic pseudocereal with a high protein content

Identification of compounds produced by reactions of flavonoids and acetaldehyde in wine

During aging, wine consumes small amounts of oxygen. This oxygen intake triggers a series of reactions that lead to flavonoid elongation, which is known to reduce bitterness and astringency while enhancing color stability.