IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

Abstract

There is evidence in the literature that non-volatile wine matrix can modify the release and therefore the perception of the compounds involved in wine aroma [1-3]. The aim of the present study is to make an estimation of the nature of these changes by using a standard volatile composition added to different real wine matrices and then analyze the headspace above them.The analytical methodology is based on a previously developed DHS-TD-GC-MS method [4]. This analytical method provides a snapshot of the contents in wine vapors and allows a better understanding of the headspace profile changes. To study the influence of the wine matrix on the release of volatile compounds, the non-volatile matrix from six different wines was isolated and all volatile compounds removed. The non-volatile matrices were used to reconstitute the six original wines but this time the volatile composition was a standard aroma solution (15 volatile compounds of different chemical families) and the same alcoholic content. The headspaces of the reconstituted wines and a model wine (12% vol. ethanol, pH 3.5) were analyzed and compared at two different moments: just after wine pouring (t=0 min) and after 10 min with glass shaking (t=10 min). The analyses were triplicated for each model wine. Also, free and total sulfur dioxide, total polyphenol index, total acidity, pH, dry mass and contents on copper, iron and zinc were determined for each wine matrix.The data collected was studied according to the time spent after wine pouring, as this factor substantially modifies the headspace of most volatile compounds. The results of a one-way ANOVA to assess the influence of the wine matrix on the initial headspace composition showed significant differences for all compounds except ethyl decanoate. Dimethyl sulfide presented marked differences among wines matrices and a significant linear anti-correlation with the copper content of the matrices. Esters showed a similar trend in the release across wine matrices, although one wine was consistently releasing lower contents of ethyl esters. Butyric and hexanoic acids were the compounds with more marked differences in release, although other compounds like β-damascenone also displayed significant differences according to the wine matrix. The variation on the release of more polar and heavier compounds, like linalool, 4-ethylphenol or vanillin in the studied matrices was more similar to that of the model wine. Only in the matrix of a young red wine a salting-out effect was detected. The data obtained in this work proves that the same volatile composition in the liquid phase of very dissimilar non-volatile wine matrices produces a headspace profile above the wines that can be significantly different and, therefore, can undoubtedly influence the perception of wine aroma.

References

[1] D.-M. Jung, S.E. Ebeler, Headspace Solid-Phase Microextraction Method for the Study of the Volatility of Selected Flavor Compounds, (2003) 6.
[2] M.-P. Sáenz-Navajas, E. Campo, L. Culleré, P. Fernández-Zurbano, D. Valentin, V. Ferreira, Effects of the Nonvolatile Matrix on the Aroma Perception of Wine, J. Agric. Food Chem. 58 (2010) 5574–5585. https://doi.org/10.1021/jf904377p.
[3] J.J. Rodríguez-Bencomo, C. Muñoz-González, I. Andújar-Ortiz, P.J. Martín-Álvarez, M.V. Moreno-Arribas, M.Á. Pozo-Bayón, Assessment of the effect of the non-volatile wine matrix on the volatility of typical wine aroma compounds by headspace solid phase microextraction/gas chromatography analysis, J. Sci. Food Agric. 91 (2011) 2484–2494. https://doi.org/10.1002/jsfa.4494.
[4] Y. Wen, R. Lopez, V. Ferreira, An automated gas chromatographic-mass spectrometric method for the quantitative analysis of the odor-active molecules present in the vapors emanated from wine, J. Chromatogr. A. 1534 (2018) 130–138. https://doi.org/10.1016/j.chroma.2017.12.064.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Lopez Ricardo¹, Wen Yan¹and Ferreira Vicente¹

¹Laboratory for Aroma Analysis and Enology, Instituto Agroalimentario de Aragón (IA2), Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza

Contact the author

Keywords

headspace, aroma release, flavor-matrix interactions, wine, GC-MS

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Phenolic composition of Tempranillo Blanco grapes changes after foliar application of urea

Our research aimed to determine the effect and efficiency of foliar application of urea on the phenolic composition of Tempranillo Blanco grapes. The field experiment was carried out in 2019 and 2020 seasons and the plot was located in D.O.Ca Rioja (North of Spain). The vineyard was Vitis vinifera L. Tempranillo Blanco and grafted on Richter-110 rootstock. The treatments were control (C), whose plants were sprayed with water and three doses of urea: plants were sprayed with urea 3 kg N/ha (U3), 6 kg N/ha (U6) and 9 kg N/ha (U9). The applications were performed in two phenological stages, pre-veraison (Pre) and veraison (Ver). Also, each of the treatments was repeated one week later. Control and treatments were performed in triplicate and arranged in a randomised block design. Grapes were harvested at optimum ripening stage. High-performance liquid chromatography was used to analyse the phenolic composition of the grapes. Finally, the results obtained from the analytical determinations – flavonols, flavanols and non-flavonoid (hydroxybenzoic acids, hydroxycinnamic acids and stilbenes) – were studied statistically by analysis of variance. The results showed that, in 2019, U6-Pre and U9-Pre treatments increased the hydroxybenzoic acid content in grapes, and also all foliar treatments applied at Pre enhanced the stilbene concentration. Moreover, U3-Ver was the only treatment that rose flavonol and stilbene contents in the Tempranillo Blanco grapes. In 2020, all treatments applied at Pre enhanced the flavonol concentration in grapes. Furthermore, U3-Pre and U9-Pre treatments increased stilbene content in grapes. Nevertheless, the hydroxybenzoic acid content was improved by U6-Ver and U9-Ver and besides, hydroxycinnamic acid concentration in grapes was increased by all treatments applied at Ver. In conclusion, the lower and highest dose of urea (U3 and U9), applied at pre-veraison, were the best treatments to improve the Tempranillo Blanco grape phenolic composition.

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Modeling the suitability of Pinot Noir in Oregon’s Willamette Valley in a changing climate

Air temperature is the key driver of grapevine phenology and a significant environmental factor impacting yield and quality for a winegrape growing region. In this study the optimal downscaled CMIP5 ensemble for computing thegrowing season average temperature (GST) viticulture climate classification index was determined to spatially compute on a decadal basis predictions of the GST climate index and the grapevine sugar ripeness (GSR) model for Pinot Noir throughout the Willamette Valley (WV) American Viticultural Area (AVA). Forecasts for average temperature and a 220 g/L target sugar concentration level were computed using daily Localized Constructed Analogs (LOCA) downscaled CMIP5 historic and Representative Concentration Pathways (RCP) future climate projections of minimum and maximum daily temperature. We explore spatiotemporal trends of the GST climate classification index and Pinot Noir specific applications of the GSR phenology model for the WV AVA. Spatiotemporal computations of the GST climate index and Pinot Noir specific applications of the GSR model enable the opportunity to explore relationships between their computed values with one intent being to provide updated GST ranges that better align with current temperature-based modeling understanding of Pinot Noir grapevine phenology and the viticultural application of LOCA CMIP5 climate projections for the WV AVA. The Pinot Noir specific applications of the GSR model or the GST index with updated bounds indicate that the percent of the WV AVA area suitable for Pinot Noir production is currently at or near its peak value in the upper 80s to lower 90s of this century.