terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Abstract

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines. Besides, the use of wine lees has been reported to stimulate MLF of O. oeni in wine [1]. Altogether, the aim of this work was to evaluate the MLF performance of L. plantarum in red grape must at different pHs, ethanol concentrations, and in the presence or absence of wine lees.

The results showed that L. plantarum can perform MLF even at pH 2.5. However, in presence of 6% (vol/vol) of ethanol, which can correspond to an early AF stage, MLF failed below pH 3.25. This behavior was observed in two commercial strains. However, the presence of wine lees in grape must at pH 3.25 allowed the completion of MLF. The addition of wine lees to the preinoculum or to the grape must also enhanced bacterial survival. Indeed, the supplementation in the preinoculum allowed the depletion of L-malic acid with one strain. In summary, wine lees potentially increase the survival of L. plantarum in red grape must, and thus, enhance MLF performance.

Acknowledgements: this work was supported by grant PID2021-124943OB-I00 (Spanish Research Agency). Aitor Balmaseda is a Margarita Salas postdoc researcher (2021URVMS25, Spanish Ministry of Universities financed with European Union-NextGenerationEU).

References:

1)  Balmaseda A. et al. (2021). Simulated lees of different yeast species modify the performance of malolactic fermentation by Oenococcus oeni in wine-like medium. Food Microbiology, 99, 103839.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Aitor Balmaseda1, *, Albert Bordons1, Nicolas Rozès2, Cristina Reguant1

1Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Grup de Biotecnologia Enològica, C/ Marcellí Domingo 1, 43007 Tarragona, Catalonia, Spain
2Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Grup de Biotecnologia Microbiana dels Aliments, C/ Marcellí Domingo 1, 43007 Tarragona, Catalonia, Spain

Contact the author*

Keywords

Lactiplantibacillus plantarum, malolactic fermentation, wine lees, must

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Study of Spanish wine sensory analysis data over a 3-year period

This study presents an investigation based on sensory analysis data of Spanish wines with geographical indications collected over a three-year period. Sensory analysis plays a crucial role in assessing the quality, characteristics, and perception of wines. The trained tasting panel at Dolmar Laboratory, accredited for objective sensory evaluation of wines since 2016, has been tasting over 5000 wines. However, it is since 2021, when a computer application for tastings was developed, that the digitalization of data allows for detailed statistical analysis of the results.

Effect of soil particle size on vine water status, leaf ABA content and berry quality in Nebbiolo grapes

The root and shoot abscisic acid (ABA) accumulation in response to water deficit and its relation with stomatal conductance is longtime known in grapevine. ABA-dependent and ABA-independent signalling response to osmotic stress coexist in sessile plants. In grapevine, the signaling role of ABA in response to water stress conditions and its influence on berry quality is critical to manage grapevine acclimation to climate change.

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).

Effect on the grape and wine characteristics of cv. Tempranillo at 3 production levels

The vineyard has experienced a general increase in yields mainly due to the elevated use of technology which caused a quality loss of grapes in more than one case. A large percentage of the Spanish vineyard is covered by a Denomination of Origin which limits the productive level of the vineyards as one of its regulations. The maximum production limit is a variable characteristic of each vineyard and is not usually regulated by agronomic criteria, and this explains the fact that each vineyard can reach high quality with a totally different yield from that set by the Denomination of Origin.

Impact of temperature and solar radiation on grape composition variability in the Saint-Emilion winegrowing area 

Grape composition is strongly influenced by climate conditions. Their expected modifications in near future, notably because of increased temperatures, could significantly modify the biochemical composition of berries at harvest, and thus wine typicity and quality. Elevated temperatures favor sugar accumulation in grapes, enhance malic acid degradation and modify the amino acid content. They also reduce significantly anthocyanin accumulation in Merlot, leading to the imbalance between anthocyanins and sugars, while no significant effects on final anthocyanin levels were reported in Tempranillo[1] and finally affect aromas or aroma precursors.