Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Oligosaccharides from Vitis vinifera grape seeds: a focus on gentianose as a novel bioactive compound

Oligosaccharides from Vitis vinifera grape seeds: a focus on gentianose as a novel bioactive compound

Abstract

AIM: Grape seeds (Vitis vinifera) are among the main constituents of grape pomace, also exploited in ingredients for nutraceutics and cosmeceutics, particularly regarding the phenolic fraction. The macromolecules of grape/wine include polyphenols, proteins and polysaccharides. Polysaccharides have been comprehensively studied because of their importance (technological and sensory properties in wines). Unlike polysaccharides, oligosaccharides have only recently been characterised. Following a concise focus about the polysaccharide composition of grape seeds, in this work we describes the purification and the identification of low molecular weight saccharides contained in the aqueous extract of grape seeds.

METHODS: A sequential two-step purification by size exclusion chromatography was carried out to fractionate compounds according to molecular weights. Chemical characterization of the combined fractions was performed by Magnetic Resonance Spectroscopy analysis and by high-resolution accurate-mass (Orbitrap mass analyzer).

RESULTS: Apart from sucrose and glucose, a fraction containing primarily a trisaccharide has been detected. Acetylation allowed the purification of the trisaccharide by flash chromatography. Structural determination on the acetylated derivative revealed the trisaccharide gentianose, a predominant carbohydrate reserve in storage roots of perennial Gentiana lutea, poorly discovered in other genera.

CONCLUSIONS:

The identification of gentianose, in grape seeds, could open new studies related to its biological functions, as well as to confirm its potential as prebiotic compound, as suggested by preliminary works.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Matteo Bordiga, Daniela IMPERIO. Fabiano TRAVAGLIA, Jean Daniel, Luigi PANZA, Marco ARLORIO

Department of Pharmaceutical Sciences, Università degli Studi del Piemonte Orientale “A. Avogadro”. Largo Donegani 2, 28100 Novara, Italy.

Contact the author

Citation

Related articles…

Water and physiological response to early leaf removal of cv. Verdejo in rainfed conditions, at different times of the day, in the D.O. Rueda (Spain)

Aim: Early leaf removal, generally applied before flowering, is mostly conceived as a technique to control grape yield and improve the health of grapes and focused on the final objective of increasing wine quality.

Coping with heatwaves: management strategies for berry survival and vineyard resilience

Climate change is leading to an increase in average temperature and in the frequency and severity of heatwaves that is already significantly affecting grapevine phenology and berry composition (Webb et al., 2010). This is compounded by water stress, which is well known to increase the vulnerability of grapevines and berries to heatwaves. In hot climate regions like australia, grape production is only possible due to relatively secure supplies of water for irrigation. However, the upper temperature limits for berry survival of well-watered grapevines remains to be tested.

Biodiversity in the vineyard agroecosystem: exploring systemic approaches

Biodiversity conservation and restoration are essential for guarantee the provision of ecosystem services associated to vineyard agroecosystem such as climate regulation trough carbon sequestration and control of pests and diseases. Most of published research dealing with the complexity of the vineyard agroecosystems emphasizes the necessity of innovative approaches, including the integration of information at different temporal and spatial scales and development of systemic analysis based on modelling. A biodiversity survey was conducted in the Franciacorta wine-growing area (Lombardy, Italy), one of the most important Italian wine-growing regions for sparkling wine production, considering a portion of the territory of 112 ha. The area was divided into several Environmental Units (EUs), defined as a whole vineyard or portion of vineyard homogenous in terms of four agronomic characteristics: planting year, planting density, cultivar, and training system. In each EU a set of compartments was identified and characterised by specific variables. The compartments are meteorology, morphology (altitude, slope, aspect, row orientation, and solar irradiance), ecological infrastructures and management. The landscape surrounding EU was also characterised in terms of land-use in a buffer zone of 500 m. For each component a specific methodology was identified and applied. Different statistical approaches were used to evaluate the method to integrate the information related to different compartments within the EU and related to the buffer zone. These approaches were also preliminarily evaluated for their ability to describe the contribution of biodiversity and landscape components to ecosystem services. This methodological exploration provides useful indication for the development of a fully systemic approach to structural and functional biodiversity in vineyard agroecosystems, contributing to promote a multifunctional perspective for the all wine-growing sector.

Methyl Jasmonate Versus Nano-Methyl Jasmonate: Effect On The Stilbene Content In Monastrell Variety

Stilbenes, a kind of non-flavonoid phenolic compounds, have been reported to be responsible for various beneficial effects. Their biological properties include antibacterial and antifungal effects, as well as cardioprotective, neuroprotective and anticancer actions (Guerrero et al. 2009).Several strategies can be used to increase stilbene content in grapes and one of them could be the use of elicitors such as methyl jasmonate. The use of this elicitor has been proven to be efficient in the production of secondary metabolites which increases the quality of wines, but its use also has some drawbacks such as its low water solubility, high volatility, and its expensive cost (Gil-Muñoz et al. 2021).

The role of terroir in tourism led amenity migration: contrasting effects in Tuscany and the Okanagan valley of British Columbia

Definitions of terroir elude consistent agreement. As defined geographical space the common denominators of its conceptualization include natural and cultural elements of life