WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 4 - WAC - Oral presentations 9 Oak Tannin and Unoaked and Oaked Wine Extracts Influence Gene Expression in HepaRG Human Liver Cells

Oak Tannin and Unoaked and Oaked Wine Extracts Influence Gene Expression in HepaRG Human Liver Cells

Abstract

Previous work from our laboratory has shown that both a purified toasted oak powder and extracts made from unoaked and oaked red wines influenced physiological parameters, metabolism and hepatic gene expression in high-fat fed C57/BL6J male mice (Luo et al., 2020).  Impacted pathways included glucose metabolism, liver fat accumulation, markers of chronic inflammation, and expression of the Gsta1 mRNA.  

In the present study, we utilized a novel cell model in a cell culture system, the HepaRG cell line.  These cells more closely resemble isolated human hepatocytes, and in particular, express robust levels of nuclear hormone receptors which may be involved in the sensing of phytochemicals.  We directly exposed HepaRG cells to three mixtures, the toasted oak tannin powder (OT), and two de-alcoholized extracts made from identical red wines fermented and aged in either steel tanks or oak barrels (oaked and unoaked wine concentrates; OWC & UWC).  In addition, other groups of cells were exposed to purified, individual compounds that may either be present in oaked wines or biotransformed by enterocytes of the small intestine:  ellagic acid (EA) and urolithin B (UroB).  OT concentration was 10 mg/L and OWC and UWC was 0.2 mL/L.  EA concentration was 300ug/L and UroB was 200ug/L.  Cells were exposed for a period of 48 hours, after which total RNA was isolated and used to perform ClariomB microarray gene expression analysis.  Data from these analyses is shown as both lists of most up- and down-regulated genes vs. untreated controls; with Venn diagrams to show commonality between different treatments, and upset plot analyses.

DOI:

Publication date: June 14, 2022

Issue: WAC 2022

Type: Article

Authors

Neil F. Shay, Tedd Goldfinger, Ting Luo, Mikayla Chen

Presenting author

Neil F. Shay – Oregon State University

 Desert Heart Foundation | Nanchang University | Oregon State University

Contact the author

Keywords

Nutrition, Gene Expression, Oak, Tannins, Ellagic Acid

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Discrimination of monovarietal Italian red wines using derivative voltammetry

Identification of specific analytical fingerprints associated to grape variety, origin, or vintage is of great interest for wine producers, regulatory agencies, and consumers. However, assessing such varietal fingerprint is complex, time consuming, and requires expensive analytical techniques. Voltammetry is a fast, cheap, and user-friendly analytical tool that has been used to investigate and measure wine phenolics.

Yeast diversity in Vitis labrusca l. Ecosystems

Although there are detailed studies on the microbiota of Vitis vinifera L. grapes, little is known about the diversity of yeast communities present in non-vinifera Vitis ecosystems (i.e., grapes and spontaneously fermenting grape musts). Potentially scientific and/or enological valuable yeast strains from these non-vinifera Vitis ecosystems might never be isolated from V. vinifera L. Using a standard culture-dependent strategy, we studied the population of yeast species during initial stages of spontaneous fermentation of V. labrusca L. (Isabella) grape musts. Rare non-Saccharomyces yeast species were recognized in Isabella, including Candida azymoides, Pichia cecembensis, Candida californica, Candida bentonensis, Issatchenkia hanoiensis and Candida apicola.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.

Characterization of 25 white grape varieties from the variety collection of ICVV (D.O.Ca.Rioja, Spain)

The effects of climate change produce an increase in sugar concentration and a decrease in acidity, without reaching the optimum grape phenolic maturity [1]. The aim of this work was to characterize 25 white grape varieties

Towards microbiota-based disease management: analysis of grapevine microbiota in plots with contrasted levels of downy mildew infection

Vineyards harbor a myriad of microorganisms that interact with each other and with the grapevines. Some microorganisms are plant pathogens, such as the oomycete Plasmopara viticola that causes grapevine downy mildew. Others, such as plant growth promoting bacteria and disease biocontrol agents, have a positive influence on vine health. The present study aims to (1) investigate whether vine-based culture media increase the cultivability of the grapevine microbiota, in comparison to standard culture media and (2) identify and isolate bacterial taxa naturally present in grapevine leaves and significantly more abundant in plots showing low susceptibility to downy mildew.