WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 4 - WAC - Oral presentations 9 Oak Tannin and Unoaked and Oaked Wine Extracts Influence Gene Expression in HepaRG Human Liver Cells

Oak Tannin and Unoaked and Oaked Wine Extracts Influence Gene Expression in HepaRG Human Liver Cells

Abstract

Previous work from our laboratory has shown that both a purified toasted oak powder and extracts made from unoaked and oaked red wines influenced physiological parameters, metabolism and hepatic gene expression in high-fat fed C57/BL6J male mice (Luo et al., 2020).  Impacted pathways included glucose metabolism, liver fat accumulation, markers of chronic inflammation, and expression of the Gsta1 mRNA.  

In the present study, we utilized a novel cell model in a cell culture system, the HepaRG cell line.  These cells more closely resemble isolated human hepatocytes, and in particular, express robust levels of nuclear hormone receptors which may be involved in the sensing of phytochemicals.  We directly exposed HepaRG cells to three mixtures, the toasted oak tannin powder (OT), and two de-alcoholized extracts made from identical red wines fermented and aged in either steel tanks or oak barrels (oaked and unoaked wine concentrates; OWC & UWC).  In addition, other groups of cells were exposed to purified, individual compounds that may either be present in oaked wines or biotransformed by enterocytes of the small intestine:  ellagic acid (EA) and urolithin B (UroB).  OT concentration was 10 mg/L and OWC and UWC was 0.2 mL/L.  EA concentration was 300ug/L and UroB was 200ug/L.  Cells were exposed for a period of 48 hours, after which total RNA was isolated and used to perform ClariomB microarray gene expression analysis.  Data from these analyses is shown as both lists of most up- and down-regulated genes vs. untreated controls; with Venn diagrams to show commonality between different treatments, and upset plot analyses.

DOI:

Publication date: June 14, 2022

Issue: WAC 2022

Type: Article

Authors

Neil F. Shay, Tedd Goldfinger, Ting Luo, Mikayla Chen

Presenting author

Neil F. Shay – Oregon State University

 Desert Heart Foundation | Nanchang University | Oregon State University

Contact the author

Keywords

Nutrition, Gene Expression, Oak, Tannins, Ellagic Acid

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Genetic study of wild grapevines in La Rioja region

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestris grapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys.

New molecular evidence of wine yeast-bacteria interaction unraveled by untargeted metabolomic profiling

Bacterial malolactic fermentation (MLF) has a considerable impact on wine quality. The yeast strain used for primary fermentation can consistently stimulate (MLF+ phenotype) or inhibit (MLF- phenotype) malolactic bacteria and the MLF process as a function of numerous winemaking practices, but the molecular evidence behind still remains a mystery. In this study, such evidence was elucidated by the direct comparison of extracellular metabolic profiles of MLF+ and MLF- yeast phenotypes. Untargeted metabolomics combining ultrahigh-resolution FT-ICR-MS analysis, powerful machine learning methods and a comprehensive wine metabolite database, discovered around 800 putative biomarkers and 2500 unknown masses involved in phenotypic distinction.

Enhancing sustainability in viticulture through digital technologies: A case study from Smyrnakis winery

The integration of digital technologies in vineyard management offers substantial opportunities for enhancing sustainability, efficiency, and grape quality.

Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties

The effects of climate change in viticulture are currently a major concern, with heat waves and drought affecting yield, wine quality, and in extreme cases, even plant survival. Ancient grapevine varieties have high intravarietal genetic variability that so far has been explored successfully to improve yield and must quality. Currently, there is little information available on intravarietal variability regarding responses to stress. In the current work, the intravarietal genetic variability of several Portuguese varieties was studied for yield, must quality, and tolerance to abiotic stress, through indirect, rapid, and nondestructive measurements carried out in the field.

New tool to evaluate color modifications during oxygen consumption in white and red wines

Measuring the effect of oxygen consumption on the color of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine can consume without significantly altering its color. The changes produced in wine after being exposed to high oxygen concentrations have been studied by different authors, but in all cases the wine has been analyzed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen[1,2].