WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 4 - WAC - Oral presentations 9 Oak Tannin and Unoaked and Oaked Wine Extracts Influence Gene Expression in HepaRG Human Liver Cells

Oak Tannin and Unoaked and Oaked Wine Extracts Influence Gene Expression in HepaRG Human Liver Cells

Abstract

Previous work from our laboratory has shown that both a purified toasted oak powder and extracts made from unoaked and oaked red wines influenced physiological parameters, metabolism and hepatic gene expression in high-fat fed C57/BL6J male mice (Luo et al., 2020).  Impacted pathways included glucose metabolism, liver fat accumulation, markers of chronic inflammation, and expression of the Gsta1 mRNA.  

In the present study, we utilized a novel cell model in a cell culture system, the HepaRG cell line.  These cells more closely resemble isolated human hepatocytes, and in particular, express robust levels of nuclear hormone receptors which may be involved in the sensing of phytochemicals.  We directly exposed HepaRG cells to three mixtures, the toasted oak tannin powder (OT), and two de-alcoholized extracts made from identical red wines fermented and aged in either steel tanks or oak barrels (oaked and unoaked wine concentrates; OWC & UWC).  In addition, other groups of cells were exposed to purified, individual compounds that may either be present in oaked wines or biotransformed by enterocytes of the small intestine:  ellagic acid (EA) and urolithin B (UroB).  OT concentration was 10 mg/L and OWC and UWC was 0.2 mL/L.  EA concentration was 300ug/L and UroB was 200ug/L.  Cells were exposed for a period of 48 hours, after which total RNA was isolated and used to perform ClariomB microarray gene expression analysis.  Data from these analyses is shown as both lists of most up- and down-regulated genes vs. untreated controls; with Venn diagrams to show commonality between different treatments, and upset plot analyses.

DOI:

Publication date: June 14, 2022

Issue: WAC 2022

Type: Article

Authors

Neil F. Shay, Tedd Goldfinger, Ting Luo, Mikayla Chen

Presenting author

Neil F. Shay – Oregon State University

 Desert Heart Foundation | Nanchang University | Oregon State University

Contact the author

Keywords

Nutrition, Gene Expression, Oak, Tannins, Ellagic Acid

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Système de Classification Climatique Multicritères (CCM) Géoviticole

Le travail concerne en premier la méthodologie de caractérisation du climat des vignobles, à l’échelle du macroclimat des régions viticoles du monde (géoviticulture). Trois indices climatiques viticoles synthétiques

Heatwaves impacts on grapevine physiology, berry chemistry & wine quality

Climate change impacts on both yields and quality have increased over the past decades, with the effects of extreme climate events having the most dramatic and obvious impacts. Increasing length and intensity of heatwaves associated with increased water stress necessitates a reevaluation of climate change responses of grapevine and, ultimately, a reconsideration of vineyard management practices under future conditions. Here we summarize results from a three-year field trial manipulating irrigation prior to and during heatwave events to assess impacts of water application rates on vine health and physiology, berry chemistry, and wine quality. We also highlight potential mitigation strategies for extreme heat, both in terms of water application, as well as other cultural practices that could be widely applicable.

New tool to evaluate color modifications during oxygen consumption in white and red wines

Measuring the effect of oxygen consumption on the color of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine can consume without significantly altering its color. The changes produced in wine after being exposed to high oxygen concentrations have been studied by different authors, but in all cases the wine has been analyzed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen[1,2].

Enhancement of the terroir

The terroir is today the most important factor of production and development in the wine sector. In a context where the commercial challenge is taking place all over the place, the distinction between traditional and “new” producing countries is not only a geographical, cultural and technical counter position but also, and above all, a legal one. Indeed, the system of standards present in the “old world” (plantation rights, production decrees, yields per hectare, etc.) which may represent, in the short term on the global market, constraints to development and product innovation must become an opportunity. But threats become opportunities, if we work, from the vine to the market, via communication, more on the elements of difference than on those of affinity.

Water and nutritional savings shape non-structural carbohydrates in grapevine (Vitis vinifera L.) cuttings

Global changes and sustainability challenge researchers in saving water and nutrients. The response of woody crops, which can be forced at facing more drought events during their life, is particularly important. Vitis vinifera can be an important model for its relevance in countries subjected to climate changes and its breeding, requiring cuttings plantation and strong pruning.