IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 A methyl salicylate glycoside mapping of monovarietal Italian white wines.

A methyl salicylate glycoside mapping of monovarietal Italian white wines.

Abstract

Among the main plant secondary metabolites, glycosides have a key-role in wine chemistry. Glycosides are non-volatile complex composed of a non-sugar component (aglycone) bound to one or more carbohydrates. The hydrolysis of glycosides occurs mainly during the fermentation due to the enzymatic activity, and during the storage as a consequence of pH and temperature conditions. In the last scenario, the gradual release of aglycones contributes to the aroma evolution of wine. Methyl salicylate (MeSa) is a plant metabolite known to be a chemical marker of several cryptogamic diseases1; however, it can be also found in wines produced from healthy grapes, whose presence provides a pleasant wintergreen and balsamic nuance, especially in aged wines2,3. This volatile odor-active ester can be found, mainly bound to glycosides, into the skin and the stem of the grapes. MeSa in the free form is frequently present under the sensory threshold while in some red and white varieties it can exceed the olfactory threshold. In our previous works MeSa have been found in relevant content, both in bound and free form, in some genetically related Italian varieties such as Trebbiano di Lugana, Trebbiano di Soave (both employed in the production of Lugana wines), and Verdicchio. In this research a straightforward filter-and-shot LC-MS/MS method was used for the determination of 7 different MeSa glycosides in 246 samples representative of 18 different monovarietal Italian white wines. Thanks to the minimized sample preparation procedure (wines were only filtered at 0.45 µm) this method allowed a reliable quantification of the analytes without wasting time, energy, and solvents, in total agreement with the Green Analytical Chemistry principles. Analysis were performed using an AB Sciex QTrap 6500+ both in positive and negative mode, equipped with a Waters Acquity C18 HSS-T3 150 mm x 2.1 mm x 1.8 µm column working at 0.28 mL*min-1. Glycosides of interest were MeSa 2-O-β-D-glucoside, MeSa 2-O-α-L-arabinopyranosyl(1à6)-β-D glucopyranoside, MeSa 2-O-β-D-xylopyranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-β-D-apiofuranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-α-L-rhamnopyranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-β-D-glucopyranosyl(1à6)-β-D-glucopyranoside, and MeSa 2-O-β-D-xylnopyranosyl(1à2)[O-β-D-xylopyranosyl(1à6)]-O-β-D-glucopyranoside. MeSa glycosides were found in Verdicchio and Lugana wines, in accordance with literature2,3, whereas where found for the first time in Garganega and Erbaluce varieties. The knowledge of the concentration of MeSa glycosides could be considered a potential predictor of the potential balsamic evolution of white wines. Further details are currently under investigation. Acknowledgments: MIUR project PRIN n. 2017RXFFRR.

References

1 Poitou, Xavier, Pascaline Redon, Alexandre Pons, Emilie Bruez, Laurent Delière, Axel Marchal, Céline Cholet, Laurence Geny-Denis, and Philippe Darriet. 2021. “Methyl Salicylate, a Grape and Wine Chemical Marker and Sensory Contributor in Wines Elaborated from Grapes Affected or Not by Cryptogamic Diseases.” Food Chemistry 360 (October): 130120. https://doi.org/10.1016/j.foodchem.2021.130120.
2 Carlin, Silvia, Domenico Masuero, Graziano Guella, Urska Vrhovsek, and Fulvio Mattivi. 2019. “Methyl Salicylate Glycosides in Some Italian Varietal Wines.” Molecules 24 (18): 3260. https://doi.org/10.3390/molecules24183260.
3 Slaghenaufi, Davide, Giovanni Luzzini, Jessica Samaniego Solis, Filippo Forte, and Maurizio Ugliano. 2021. “Two Sides to One Story—Aroma Chemical and Sensory Signature of Lugana and Verdicchio Wines.” Molecules 26 (8): 2127. https://doi.org/10.3390/molecules26082127.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Piergiovanni Maurizio1, Carlin Silvia2, Masuero Domenico2, Rolle Luca3, Rio Segade Susana3, Slaghenaufi Davide4, Ugliano Maurizio4, Marangon Matteo5, Curioni Andrea5, Parpinello Giuseppina Paola6, Versari Andrea6, Piombino Paola7, Pittari Elisabetta7, Mattivi Fulvio1 and Vrhovsek Urska2

1Center Agriculture Food Environment (C3A), University of Trento
2Metabolomics Unit, Research and Innovation Center, Edmund Mach Foundation, Italy
3Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Italy
4Department of Biotechnology, University of Verona, Italy
5Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
6Department of Agricultural and Food Sciences, University of Bologna, Italy
7Department of Agricultural Sciences, University of Naples Federico II, Italy

Contact the author

Keywords

Methyl salicylate, glycosides, aglycones, monovarietal, white-wines 

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Traditional agroforestry vineyards, sources of inspiration for the agroecological transition of viticulture

A unique “terroir” can be found in southern Bolivia, which combines the specific features of climate, topography and altitude of high valleys, with the management of grapevines staked on trees. It is one of the rare remnants of agroforestry viticulture. A survey was carried out among 29 grapegrowers in three valleys, to characterize the structure and management of these vineyards, and identify the services they expect from trees. Farms were small (2.2 ha on average) and 85% of vineyards were less than 1 ha. Viticulture was associated with vegetable, fruit and fodder production, sometimes in the same fields. Molle trees were found in all plots, together with one or two other native tree species. Traditional grapevine varieties such as Negra Criolla, Moscatel de Alejandría and Vicchoqueña were grown with a large range of densities from 1550 to 9500 vines ha-1. From 18 to 30% of them were staked on trees, with 1.2 to 4.9 vines per tree. The management of these vineyards (irrigation, fertilization and grapevine protection) was described, the most particular technical operation being the coordinated pruning of trees and grapevines. Three types of management could be identified in the three valleys. Grapegrowers had a clear idea of the ecosystem services they expected from trees in their vineyards. The main one was protection against climate hazards (hail, frost, flood). Then they expected benefits in terms of pest and disease control, improvement of soil fertility and resulting yield. At last, some producers claimed that tree-staking was quicker and cheaper than conventional trellising. It can be hypothesized then that agroforestry is a promising technique for the agroecological transition of viticulture. Its contribution to the “terroir” of the high valleys of southern Bolivia and its link with the specificities of the wines and spirits produced there remain to be explored.

VINIoT – Precision viticulture service

The project VINIoT pursues the creation of a new technological vineyard monitoring service, which will allow companies in the wine sector in the SUDOE space to monitor plantations in real time and remotely at various levels of precision. The system is based on spectral images and an IoT architecture that allows assessing parameters of interest viticulture and the collection of data at a precise scale (level of grape, plant, plot or vineyard) will be designed. In France, three subjects were specifically developed: evaluation of maturity, of water stress, and detection of flavescence dorée. For the evaluation of maturity, it has been decided first to work at the berry scale in the laboratory, then at the bunch scale and finally in the vineyard. The acquisition of the spectral hyperstal image as well as the reference analyzes to measure the maturity, were carried out in the laboratory after harvesting the berries in a maturity monitoring context. This work focuses on a case study to predict sugar content of three different grape varieties: Syrah, Fer Servadou and Mauzac. A robust method called Roboost-PLSR, developed in the framework of this work (Courand et al., 2022), to improve prediction model performance was applied on spectra after the acquirement of hyperspectral images. Regarding the evaluation of water stress, to work with a significant variability in terms of water status, it has been worked first with potted plants under 2 different water regimes. The facilities have allowed the supervision of irrigation and micro-climatic conditions. The regression models on agronomic variables (stomatal conductance, water potential, …) are studied. To detect flavescence dorée, the experimental plan has consisted of work at leaf scale in the laboratory first, and then in the field. To detect the disease from hyper-spectral imaging, a combination of multivariate curve resolution-alternating least squares (MCR-ALS) and factorial discriminant analysis (FDA) was proposed. This strategy proved the potential towards the discrimination of healthy and infected leaves by flavescence dorée based on the use of hyperspectral images (Mas Garcia et al., 2021).

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.