IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 A methyl salicylate glycoside mapping of monovarietal Italian white wines.

A methyl salicylate glycoside mapping of monovarietal Italian white wines.

Abstract

Among the main plant secondary metabolites, glycosides have a key-role in wine chemistry. Glycosides are non-volatile complex composed of a non-sugar component (aglycone) bound to one or more carbohydrates. The hydrolysis of glycosides occurs mainly during the fermentation due to the enzymatic activity, and during the storage as a consequence of pH and temperature conditions. In the last scenario, the gradual release of aglycones contributes to the aroma evolution of wine. Methyl salicylate (MeSa) is a plant metabolite known to be a chemical marker of several cryptogamic diseases1; however, it can be also found in wines produced from healthy grapes, whose presence provides a pleasant wintergreen and balsamic nuance, especially in aged wines2,3. This volatile odor-active ester can be found, mainly bound to glycosides, into the skin and the stem of the grapes. MeSa in the free form is frequently present under the sensory threshold while in some red and white varieties it can exceed the olfactory threshold. In our previous works MeSa have been found in relevant content, both in bound and free form, in some genetically related Italian varieties such as Trebbiano di Lugana, Trebbiano di Soave (both employed in the production of Lugana wines), and Verdicchio. In this research a straightforward filter-and-shot LC-MS/MS method was used for the determination of 7 different MeSa glycosides in 246 samples representative of 18 different monovarietal Italian white wines. Thanks to the minimized sample preparation procedure (wines were only filtered at 0.45 µm) this method allowed a reliable quantification of the analytes without wasting time, energy, and solvents, in total agreement with the Green Analytical Chemistry principles. Analysis were performed using an AB Sciex QTrap 6500+ both in positive and negative mode, equipped with a Waters Acquity C18 HSS-T3 150 mm x 2.1 mm x 1.8 µm column working at 0.28 mL*min-1. Glycosides of interest were MeSa 2-O-β-D-glucoside, MeSa 2-O-α-L-arabinopyranosyl(1à6)-β-D glucopyranoside, MeSa 2-O-β-D-xylopyranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-β-D-apiofuranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-α-L-rhamnopyranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-β-D-glucopyranosyl(1à6)-β-D-glucopyranoside, and MeSa 2-O-β-D-xylnopyranosyl(1à2)[O-β-D-xylopyranosyl(1à6)]-O-β-D-glucopyranoside. MeSa glycosides were found in Verdicchio and Lugana wines, in accordance with literature2,3, whereas where found for the first time in Garganega and Erbaluce varieties. The knowledge of the concentration of MeSa glycosides could be considered a potential predictor of the potential balsamic evolution of white wines. Further details are currently under investigation. Acknowledgments: MIUR project PRIN n. 2017RXFFRR.

References

1 Poitou, Xavier, Pascaline Redon, Alexandre Pons, Emilie Bruez, Laurent Delière, Axel Marchal, Céline Cholet, Laurence Geny-Denis, and Philippe Darriet. 2021. “Methyl Salicylate, a Grape and Wine Chemical Marker and Sensory Contributor in Wines Elaborated from Grapes Affected or Not by Cryptogamic Diseases.” Food Chemistry 360 (October): 130120. https://doi.org/10.1016/j.foodchem.2021.130120.
2 Carlin, Silvia, Domenico Masuero, Graziano Guella, Urska Vrhovsek, and Fulvio Mattivi. 2019. “Methyl Salicylate Glycosides in Some Italian Varietal Wines.” Molecules 24 (18): 3260. https://doi.org/10.3390/molecules24183260.
3 Slaghenaufi, Davide, Giovanni Luzzini, Jessica Samaniego Solis, Filippo Forte, and Maurizio Ugliano. 2021. “Two Sides to One Story—Aroma Chemical and Sensory Signature of Lugana and Verdicchio Wines.” Molecules 26 (8): 2127. https://doi.org/10.3390/molecules26082127.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Piergiovanni Maurizio1, Carlin Silvia2, Masuero Domenico2, Rolle Luca3, Rio Segade Susana3, Slaghenaufi Davide4, Ugliano Maurizio4, Marangon Matteo5, Curioni Andrea5, Parpinello Giuseppina Paola6, Versari Andrea6, Piombino Paola7, Pittari Elisabetta7, Mattivi Fulvio1 and Vrhovsek Urska2

1Center Agriculture Food Environment (C3A), University of Trento
2Metabolomics Unit, Research and Innovation Center, Edmund Mach Foundation, Italy
3Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Italy
4Department of Biotechnology, University of Verona, Italy
5Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
6Department of Agricultural and Food Sciences, University of Bologna, Italy
7Department of Agricultural Sciences, University of Naples Federico II, Italy

Contact the author

Keywords

Methyl salicylate, glycosides, aglycones, monovarietal, white-wines 

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

VineyardFACE: Investigation of a moderate (+20%) increase of ambient CO2 level on berry ripening dynamics and fruit composition

Climate change and rising atmospheric carbon dioxide concentration is a concern for agriculture, including viticulture. Studies on elevated carbon dioxide have already been on grapevines, mainly taking place in greenhouses using potted plants or using field grown vines under higher CO2 enrichment, i.e. >650 ppm. The VineyardFACE, located at Hochschule Geisenheim University, is an open field Free Air CO2 Enrichment (FACE) experimental set-up designed to study the effects of elevated carbon dioxide using field grown vines (Vitis vinifera L. cvs. Riesling and Cabernet Sauvignon). As the carbon dioxide fumigation started in 2014, the long term effects of elevated carbon dioxide treatment can be investigated on berry ripening parameters and fruit metabolic composition.
The present study aims to investigate the effect on fruit composition under a moderate increase (+20%; eCO2) of carbon dioxide concentration, as predicted for 2050 on both Riesling and Cabernet Sauvignon. Berry composition was determined for primary (sugars, organic acids, amino acids) and secondary metabolites (anthocyanins). Special focus was given on monitoring of berry diameter and ripening rates throughout three growing seasons. Compared to previous results of the early adaptative phase of the vines [1], our results show little effect of eCO2 treatment on primary metabolites composition in berries. However, total anthocyanins concentration in berry skin was lower for eCO2 treatment in 2020, although the ratio between anthocyanins derivatives did not differ.
[1] Wohlfahrt Y., Tittmann S., Schmidt D., Rauhut D., Honermeier B., Stoll M. (2020) The effect of elevated CO2 on berry development and bunch structure of Vitis vinifera L. cvs. Riesling and Cabernet Sauvignon. Applied Science Basel 10: 2486

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Influence of a spontaneous cover crop on the vineyard and soil erosion under Mediterranean climate

Sixty five % of the agricultural area of the Basque Country located in the DO Ca Rioja corresponds to vineyards. More than 40% of it has an average slope greater than 10%, which makes it sensitive to erosive processes. Furthermore, it is foreseeable that extreme weather events (storms, hail, extreme heat and cold, etc.) will be favored due to climate change. Cover cropping can mitigate this risk, and therefore the objective of this work is to evaluate the impact that a vegetable cover has on the agronomic behavior of the vineyard, the quality of the grape and soil erosion. For this, a trial has been carried out with a Graciano variety vineyard with a slope between 10% -20% during the years 2020 and 2021. Conventional tillage management in the area has been compared (4-6 passes per year of tillage machinery) versus spontaneous vegetation cover management in the vineyard. This implies not tilling and allowing the grass of the land to colonize the range between the lines of vines, controlling their height through 1-3 mowing passes per year, always trying to affect the surface of the land as little as possible. The vegetative growth, yield and quality of the grape and wine was measured. Furthermore, erosion has been measured using Gerlasch boxes. The yield was lower in the second year of the trial in the cover crop treatment, but erosion was significantly reduced.