IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 A methyl salicylate glycoside mapping of monovarietal Italian white wines.

A methyl salicylate glycoside mapping of monovarietal Italian white wines.

Abstract

Among the main plant secondary metabolites, glycosides have a key-role in wine chemistry. Glycosides are non-volatile complex composed of a non-sugar component (aglycone) bound to one or more carbohydrates. The hydrolysis of glycosides occurs mainly during the fermentation due to the enzymatic activity, and during the storage as a consequence of pH and temperature conditions. In the last scenario, the gradual release of aglycones contributes to the aroma evolution of wine. Methyl salicylate (MeSa) is a plant metabolite known to be a chemical marker of several cryptogamic diseases1; however, it can be also found in wines produced from healthy grapes, whose presence provides a pleasant wintergreen and balsamic nuance, especially in aged wines2,3. This volatile odor-active ester can be found, mainly bound to glycosides, into the skin and the stem of the grapes. MeSa in the free form is frequently present under the sensory threshold while in some red and white varieties it can exceed the olfactory threshold. In our previous works MeSa have been found in relevant content, both in bound and free form, in some genetically related Italian varieties such as Trebbiano di Lugana, Trebbiano di Soave (both employed in the production of Lugana wines), and Verdicchio. In this research a straightforward filter-and-shot LC-MS/MS method was used for the determination of 7 different MeSa glycosides in 246 samples representative of 18 different monovarietal Italian white wines. Thanks to the minimized sample preparation procedure (wines were only filtered at 0.45 µm) this method allowed a reliable quantification of the analytes without wasting time, energy, and solvents, in total agreement with the Green Analytical Chemistry principles. Analysis were performed using an AB Sciex QTrap 6500+ both in positive and negative mode, equipped with a Waters Acquity C18 HSS-T3 150 mm x 2.1 mm x 1.8 µm column working at 0.28 mL*min-1. Glycosides of interest were MeSa 2-O-β-D-glucoside, MeSa 2-O-α-L-arabinopyranosyl(1à6)-β-D glucopyranoside, MeSa 2-O-β-D-xylopyranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-β-D-apiofuranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-α-L-rhamnopyranosyl(1à6)-β-D-glucopyranoside, MeSa 2-O-β-D-glucopyranosyl(1à6)-β-D-glucopyranoside, and MeSa 2-O-β-D-xylnopyranosyl(1à2)[O-β-D-xylopyranosyl(1à6)]-O-β-D-glucopyranoside. MeSa glycosides were found in Verdicchio and Lugana wines, in accordance with literature2,3, whereas where found for the first time in Garganega and Erbaluce varieties. The knowledge of the concentration of MeSa glycosides could be considered a potential predictor of the potential balsamic evolution of white wines. Further details are currently under investigation. Acknowledgments: MIUR project PRIN n. 2017RXFFRR.

References

1 Poitou, Xavier, Pascaline Redon, Alexandre Pons, Emilie Bruez, Laurent Delière, Axel Marchal, Céline Cholet, Laurence Geny-Denis, and Philippe Darriet. 2021. “Methyl Salicylate, a Grape and Wine Chemical Marker and Sensory Contributor in Wines Elaborated from Grapes Affected or Not by Cryptogamic Diseases.” Food Chemistry 360 (October): 130120. https://doi.org/10.1016/j.foodchem.2021.130120.
2 Carlin, Silvia, Domenico Masuero, Graziano Guella, Urska Vrhovsek, and Fulvio Mattivi. 2019. “Methyl Salicylate Glycosides in Some Italian Varietal Wines.” Molecules 24 (18): 3260. https://doi.org/10.3390/molecules24183260.
3 Slaghenaufi, Davide, Giovanni Luzzini, Jessica Samaniego Solis, Filippo Forte, and Maurizio Ugliano. 2021. “Two Sides to One Story—Aroma Chemical and Sensory Signature of Lugana and Verdicchio Wines.” Molecules 26 (8): 2127. https://doi.org/10.3390/molecules26082127.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Piergiovanni Maurizio1, Carlin Silvia2, Masuero Domenico2, Rolle Luca3, Rio Segade Susana3, Slaghenaufi Davide4, Ugliano Maurizio4, Marangon Matteo5, Curioni Andrea5, Parpinello Giuseppina Paola6, Versari Andrea6, Piombino Paola7, Pittari Elisabetta7, Mattivi Fulvio1 and Vrhovsek Urska2

1Center Agriculture Food Environment (C3A), University of Trento
2Metabolomics Unit, Research and Innovation Center, Edmund Mach Foundation, Italy
3Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Italy
4Department of Biotechnology, University of Verona, Italy
5Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
6Department of Agricultural and Food Sciences, University of Bologna, Italy
7Department of Agricultural Sciences, University of Naples Federico II, Italy

Contact the author

Keywords

Methyl salicylate, glycosides, aglycones, monovarietal, white-wines 

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Postveraison shoot trimming in Tannat and Merlot: preliminary results on yield components, plant balance and berry composition

There is currently a trend towards the production of wines with low alcohol content. To achieve this, grapes with low sugar content must be used. There are techniques at the vineyard level that can delay ripening and avoid excessive sugar accumulation without, a priori, affecting the final polyphenol content. Postveraison shoot trimming (PVST) is experimentally evaluated for these purposes, but its impact under Uruguayan climatic conditions with high interannual variability is not known. The aim of this work is to assess the PVST in Tannat and Merlot cultivars and their impact on yield components, plant balance and berry primary composition. In this study, two commercial vineyards of 10 years old Tannat and Merlot (grafted on SO4) at Canelones Department were selected. During the 2020-201 growing season, grapevines were submitted to PVST when grapes reached 15º Brix. In a randomized block, trimmed (T) and control (C) plants were evaluated with three repetitions each cultivar. Evaluation of the evolution of primary berry composition during ripening, measurement of yield components and plant balance were performed. For both cultivars, PVST did not affect yield components. Merlot reached 5.4 kg per plant and Tannat 7.1 kg, with not statistical significance between treatments. However, statistical differences were observed in terms of plant balance. In Merlot Ravaz Index reached a difference of 5.3 (12.0 in T and 6.7 in C) meanwhile Tannat reached 3.5 of statistical difference (13.7 in T and 10.2 in C). The tendency to imbalance for the treated plants had an impact on the final grape composition. Merlot grapes showed statistical difference in final total acidity (0.3 g of difference between treatments) while treatments impact final sugar content on Tannat grapes (10.0 g of difference between treatments). Further studies are needed to assess the impact of different canopy management techniques in our conditions.

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.

The interplay between grape ripening and weather anomalies – A modeling exercise

Current climate change is increasing inter- and intra-annual variability in atmospheric conditions leading to grapevine phenological shifts as well altered grape ripening and composition at ripeness. This study aims to (i) detect weather anomalies within a long-term time series, (ii) model grape ripening revealing altered traits in time to target specific ripeness thresholds for four Vitis vinifera cultivars, and (iii) establish empirical relationships between ripening and weather anomalies with forecasting purposes. The Day of the Year (DOY) to reach specific grape ripeness targets was determined from time series of sugar concentrations, total acidity and pH collected from a private company in the period 2009-2021 in North-Eastern Italy. Non-linear models for the DOY to reach the specified ripeness thresholds were assessed for model efficiency (EF) and error of prediction (RMSE) in four grapevine cultivars (Merlot, Cabernet Sauvignon, Glera and Garganega). For each vintage and cultivar, advances or delays in DOY to target specified ripeness thresholds were assessed with respect to the average ripening dynamics. Long-term meteorological series monitored at ground weather station by means of hourly air temperature and rainfall data were analyzed. Climate statistics were obtained and for each time period (month, bimester, quarter and year) weather anomalies were identified. A linear regression analysis was performed to assess a possible correlation that may exist between ripening and weather anomalies. For each cultivar, ripeness advances or delays expressed in number of days to target the specific ripening threshold were assessed in relation to registered weather anomalies and the specific reference time period in the vintage. Precipitation of the warmest month and spring quarter are key to understanding the effect of climate change on sugar ripeness. Minimum temperatures of May-June bimester and maximum temperatures of spring quarter best correlate with altered total acidity evolution and pH increment during the ripening process, respectively.

Aromatic maturity is a cornerstone of terroir expression in red wine

Harvesting grapes at adequate maturity is key to the production of high-quality red wines. Enologists and wine makers define several types of maturity, including technical maturity, phenolic maturity and aromatic maturity. Technical maturity and phenolic maturity are relatively well documented in the scientific literature, while articles on aromatic maturity are scarcer. This is surprising, because aromatic maturity is, without a doubt, the most important of the three in determining wine quality and typicity (including terroir expression). Optimal terroir expression can be obtained when the different types of maturity are reached at the same time, or within a short time frame. This is more likely to occur when the ripening takes place under mild temperatures, neither too cool, nor too hot. Aromatic expression in wine can be driven, from low to high maturity, by green, herbal, fresh fruit, ripe fruit, jammy fruit, candied fruit or cooked fruit aromas. Green and cooked fruit aromas are not desirable in red wines, while the levels of other aromatic compounds contribute to the typicity of the wine in relation to its origin. Wines produced in cool climates, or on cool soils in temperate climates, are likely to express herbal or fresh fruit aromas; while wines produced under warm climates, or on warm soils in temperate climates, may express ripe fruit, jammy fruit or candied fruit aromas. Growers can optimize terroir expression through their choice of grapevine variety. Early ripening varieties perform better in cool climates and late ripening varieties in warm climates. Additionally, maturity can be advanced or delayed by different canopy management practices or training systems.