WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Immobilization of S. cerevisiae and O. œni for the control of wine fermentation steps

Immobilization of S. cerevisiae and O. œni for the control of wine fermentation steps

Abstract

Controlling the speed of alcoholic (AF) and malolactic (MLF) fermentations in wine can be an important challenge for the production of certain short rotation wines for entry-level market segments. Immobilization techniques for Saccharomyces cerevisiae and Œnococcus œni, the microorganisms responsible for these fermentations, are widely studied for industrial applications. Indeed, these processes allow to accumulate biomass and thus to increase cell densities inducing high fermentation velocities. Recent works have shown the performance of MLF carried out with biofilms of O. œni, immobilized on various supports in a rich medium (MRSm: modified MRS broth with malic acid and fructose).

Our work shows the possibility of inducing the adhesion of O. œni and S. cerevisiae, alone or in co-culture, in low nutriment medium, on different materials already used in the winery environment, at the microplate scale, in static conditions. The quantities of attached biomass (CFU counts) are close for all the tested modalities at the end of the adhesion time (48h for S. cerevisiae and 72h for O. œni with one broth renewal). The inoculated supports are transferred after rinsing into tubes containing must or wine, depending on the application, and the progress of the fermentations is analysed. In most conditions, total sugars are below 5 g/l after 5 days of AF, and all malic acid in the substrate (about 4 g/l) is consumed in 10 to 15 days.

This immobilization model could be the first step towards a perfectly controlled industrial fermentation processes.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Marianne GOSSET, Patricia TAILLANDIER, , Christine ROQUES, Magali Garcia

Presenting author

Marianne GOSSET – LGC Biosym Toulouse

LGC Biosym Toulouse | LGC Biosym Toulouse | AB7 Industries

Contact the author

Keywords

Immobilization – O. oeni – S. cerevisiae – fermentations – industrial process

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Identification of caffeic acid as a major component of Moscatel wine protein sediment

Proteins play a significant role in the colloidal stability and clarity of white wines [1]. However, under conditions of high temperatures during storage or transportation, the proteins themselves can self-aggregate into light-dispersing particles causing the so-called protein haze [2]. Formation of these unattractive precipitates in bottled wine is a common defect of commercial wines, making them unacceptable for sale [3]. Previous studies identified the presence of phenolic compounds in the natural precipitate of white wine [4], contributing to the hypothesis that these compounds could be involved in the mechanism of protein haze formation.

Oenological potential of wines and agronomical characterisation of grapes from five white resistant Italian varieties at Serra Gaúcha, Southern Brazil

Rio grande do sul is the main grape producing state in Brazil, with the largest wine-growing area, responsible by 90% of the national production of wines and grape juices. Serra Gaúcha is the main vitivinicultural region, where around 15% of the area is destined to produce wines from vitis vinifera L. grapes. This region presents high rainfall during the grape maturation cycle, a factor that leads to great risk of attacks by fungal pathogens. the use of resistant varieties can reduce the cost and quantity of spraying, improving wine quality, focusing on a sustainable vitiviniculture.

L’essor des produits “No-Low” : nouveaux défis pour l’étiquetage et la réglementation

In recent years, “no-low” products seem to become a new worldwide trend. It appears to be a possible answer to the well-known context of climate change, the decline in wine consumption, and the wellness/health trend (“free from” claims, vegan, and so on…) That consumers are looking for. The aim of this study is to provide an overview of the “no-low” products sold in the french market (but not only french products), focusing on the labelling, packaging, and sales presentation of these products.

Disease‐induced alterations in the reflectance spectrum of grape leaves

Context and purpose of the study ‐ Phytopathogenic diseases impact the development and yield of grapevines, resulting in economical, social and environmental losses.

Proposta per un parco produttivo agrovitivinicolo dei “colli piacentini”

Le Dipartimento di Progettazione dell ‘Architettura del Politecnico di Milano et l’Istituto di Viticoltura della Facoltà d’Agraria di Piacenza dell’Università Cattolica del Sacra Cuore, ont elaboré une proposition pour réaliser, dans l’aire de colline de la province de Piacenza, un Parco Produttivo Agrovitivinicolo.