OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analysis and composition of grapes, wines, wine spirits 9 The fundamental role of pH in the anthocyanins chemical behavior and in their extractability during winemaking

The fundamental role of pH in the anthocyanins chemical behavior and in their extractability during winemaking

Abstract

The chemical behavior of anthocyanins is considerably affected even by slight pH variations with impor-tant implications for the winemaking as well as for the wine conservation. Considering that this is a cen-tral issue to the enological sector, we decided to better investigate the influence of pH on the anthocyanin chemistry.

Initially, by chromatographic and advanced NMR techniques the chemical behavior of malvi-din- 3-O-glucoside was studied in wine-like solutions with pH values ranging from 3 to 4. First, the already composite aqueous equilibrium of malvidin-3-O-glucoside turned out to be even more complex than so far thought, as a new hydration product of the anthocyanin was detected and characterized in solution.

More importantly on account of its technological implications, the anthocyanin solubility appeared to decrease remarkably as the pH value of the wine-like solutions increased. A dramatic drop in terms of anthocyanin solubility was observed at pH 3.32, where the measured molecule concentration was reduced to almost 25% the expected one. Also, at such pH level the anthocyanin self-association appeared significantly affected.

In more detail,the flavylium ion self-association predominant at lower pH levels was altered and found to co-occur with a preferential co-pigmentation involving flavylium ion species and the trans-chalcone form of malvidin-3-O-glucoside. At higher pH values, this latter association was the only one detected in so-lution. In the light of these results, we set up an experimental protocol with the purpose of analyzing the pH influence on the anthocyanin chemistry and extractability in real wines produced by varying their pH levels during the maceration-fermentation phases.

Preliminary chemical analysis of such wines provided data consistent with those obtained in wine-like solutions. Indeed, the extraction of malvidin-3-O-gluco-side and that of anthocyanins in general was more contained as the pH value increased.

Additionally, other molecules of enological interest, including catechins, cinnamates, syringic acid and terpenoids, turned out to be affected by the wine pH. These analytical data highlight the fundamental role of pH during the wine-making and the importance of regulating its level to obtain wines with the desired polyphenolic structure.

Boulton. American journal of enology and viticulture, 52(2), 67-87 (2001). Forino, M., Gambuti, A., Luciano, P., Moio, L. J. Agric. Food Chem. (2019) doi:10.1021/acs.jafc.8b05895 

DOI:

Publication date: June 11, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Martino Forino, Angelita Gambuti, Luigi Picariello, Luigi Moio

Department of Agricultural Sciences, University of Napoli “Federico II”−Oenology Sciences Section Viale Italia, 83100 Avellino, Italy

Contact the author

Keywords

Anthocyanin , pH, pigmentation, anthocyanin solubility

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Bio-protection by one strain of M. Pulcherrima: microbiological and chemical impacts in red wines

In oenology, bio-protection consists in adding bacteria, yeasts or a mixture of microorganisms on grape must before fermentation in order to reduce the use of chemical compounds such as sulphites.

Relationship between chemical parameters of tannins and in-mouth attributes of grape phenolic fractions

Establish relationships between taste and mouthfeel properties of grapes and tannin-related chemical parameters. Tempranillo Tinto and Garnacha Tinta grapes were harvested from distinct blocks in different dates; each sample collection date was separated by one week. Grapes were destemmed and macerated in 15% of ethanol for one week. The polyphenolic fraction (PF) of samples was submitted to solid phase extraction on C18 cartridges and recovered with ethanol. PFs were reconstituted in wine model and their taste and mouthfeel properties were characterised by rate-K-attributes methodology. In parallel, concentration (TC) and activity (TAc) of tannins as well as the concentration of tannins linked to anthocyanins (T-A) were determined using HPLC-UV–VIS.

Use of multispectral satellite for monitoring vine water status in mediterranean areas

The development of new generations of multispectral satellites such as Sentinel-2 opens possibilities as to vine water status assessment (Cohen et al., 2019). Based on a three years field campaign, a model of Stem Water Potential (SWP) estimation on vine using four satellite bands in Red, Red-Edge, NIR and SWIR domains was developed (Laroche-Pinel et al., 2021). The model relies on SWP field measures done using a pressure chamber (Scholander et al., 1965), which is a common, robust and precise method to assess vine water status (Acevedo-Opazo et al., 2008). The model was mainly developed from from SWP measures on Syrah N (Laroche Pinel E., 2021).

A large scale monitoring was organized in different vineyards in the Mediterranean region in 2021. 10 varieties amongst the most represented in this area were monitored (Cabernet sauvignon N, Chardonnay B, Cinsault N, Grenache N, Merlot N, Mourvèdre N, Sauvignon B, Syrah N, Vermentino B, Viognier B). The model was used to produce water status maps from Sentinel-2 images, starting from the beginning of June (fruit set) up to September (harvest). The average estimated SWP for each vine was compared to actual field SWP measures done by wine growers or technicians during usual monitoring of irrigation programs. The correlations between mean estimated SWP and mean measured SWP were at the same level than expected by the model. (Laroche Pinel, 2021) The general SWP kinetics were comparable. The estimated SWP would have led to same irrigation decisions concerning the date of first irrigation in comparison with measured SWP.

Acevedo-Opazo, C., Tisseyre, B., Ojeda, H., Ortega-Farias, S., Guillaume, S. (2008). Is it possible to assess the spatial variability of vine water status? OENO One, 42(4), 203.
Cohen, Y., Gogumalla, P., Bahat, I., Netzer, Y., Ben-Gal, A., Lenski, I., … Helman, D. (2019). Can time series of multispectral satellite images be used to estimate stem water potential in vineyards? In Precision agriculture ’19, The Netherlands: Wageningen Academic Publishers, pp. 445–451.
Laroche-Pinel, E., Duthoit, S., Albughdadi, M., Costard, A. D., Rousseau, J., Chéret, V., & Clenet, H. (2021). Towards vine water status monitoring on a large scale using sentinel-2 images. remote sensing, 13(9), 1837.
Laroche-Pinel,E. (2021). Suivi du statut hydrique de la vigne par télédétection hyper et multispectrale. Thèse INP Toulouse, France.
Scholander, P.F., Bradstreet, E.D., Hemmingsen, E.A., & Hammel, H.T. (1965). Sap pressure in vascular plants: Negative hydrostatic pressure can be measured in plants. Science, 148(3668), 339–346.

Sviluppo di una metodologia di tracciabilità e definizione dell’impronta petrochimica in suoli e vini della Sicilia occidentale nella piana di Marsala (TP)

I risultati delle ricerche condotte in un vigneto sperimentale di Marsala (TP), scelto per omogeneità di fattori bio-agronomici (età, tecniche colturali, potenzialità vegetativa e produttiva)

Using NIR/SWIR hyperspectral camera mounted on a UAV to assess grapevine water status in a variably irrigated vineyard

Vineyards face climate change, increasing temperatures, and drought affecting vine water status. Water deficit affects plant physiology and can ultimately decrease yield and grape quality when it is not well managed. Monitoring vine water status and irrigation can help growers better manage their vineyards.