terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties

Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties


The effects of climate change in viticulture are currently a major concern, with heat waves and drought affecting yield, wine quality, and in extreme cases, even plant survival. Ancient grapevine varieties have high intravarietal genetic variability that so far has been explored successfully to improve yield and must quality. Currently, there is little information available on intravarietal variability regarding responses to stress. In the current work, the intravarietal genetic variability of several Portuguese varieties was studied for yield, must quality, and tolerance to abiotic stress, through indirect, rapid, and nondestructive measurements carried out in the field.

The present work describes an innovative approach in selection for abiotic stress tolerance, performed in experimental populations of several varieties installed according to resolvable row-column designs with 4 to 6 replicates1. Representative samples of the intravarietal variability of three ancient varieties (Uva Cão, Castelão, and Moscatel Graúdo) under conditions of drought and extreme heat were measured for surface leaf temperature (SLT), a parameter that had already been used for the varieties Aragonez2 and Arinto3, with good results. This was complemented with analyses of yield and quality characteristics of the must. Linear mixed models were fitted to the data of the traits evaluated, and the empirical best linear unbiased predictors (EBLUPs) of genotypic effects for each trait were obtained as well as the coefficient of genotypic variation (CVG) and broad sense heritability4. The genotypes were then ranked according to their level of tolerance to abiotic stress, and the changes in yield and traits of quality of the must were assessed for those genotypes. The results obtained will be the basis to develop, for those varieties, a new type of polyclonal selected material with increased tolerance to abiotic stress, in relation to the average of the varieties.

Acknowledgements: Projects “Conservation and selection of ancient grapevine varieties” (PDR2020-784-042704), “Save the intra-varietal diversity of autochthonous grapevine varieties” (PRR-C05-i03-|-000016); FCT: DL57/2016/CP1382/CT0024 to LC; UIDB/04129/2020 and LEAF Thematic Line Project Clones4ClimateChange.

1)  Gonçalves E. et. al. (2010) Experimental designs for evaluation of genetic variability and selection of ancient grapevine varieties: a simulation study. Heredity, 104: 552–562. DOI: 10.1038/hdy.2009.153

2)  Carvalho L.C. et. al. (2020) Selecting Aragonez genotypes able to outplay climate change driven abiotic stress. Front. Plant Sci., 11: 599230, DOI: 10.3389/fpls.2020.599230

3)  Carvalho L.C. et. al. (2023) Polyclonal selection for abiotic stress tolerance in Arinto: implications in yield and quality of the must. 44th World Congress of Vine and Wine, Cádiz, Spain, 5-9 June.

4)  Gonçalves E. and Martins A. (2019). Genetic gains of selection in ancient grapevine cultivars. Acta Hortic., 1248, 47–54. DOI: 10.17660/ActaHortic.2019.1248.7


Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article


Luísa Carvalho1, Teresa Pinto2, Joana Ribeiro1, J. Miguel Costa1, Antero Martins1,2, Elsa Gonçalves1,2

1LEAF- Linking Landscape, Environment, Agriculture and Food, Associated Laboratory TERRA; Instituto Superior de Agronomia, Universidade de Lisboa, Portugal
2Associação Portuguesa para a Diversidade da Videira – PORVID, Lisboa, Portugal

Contact the author*


abiotic stress, grapevine, intravarietal variability, polyclonal selection, surface leaf temperature


2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series


Related articles…

Late pruning, an alternative for rainfed vine varieties facing new climatic conditions

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.

Symbiotic microorganisms application in vineyards: impacts on grapevine performance and microbiome

Microorganism-based inoculants have been suggested as a viable solution to mitigate the adverse effects of climate change on viticulture. However, the actual effectiveness of these inoculants when applied under field conditions remains a challenge, and their effects on the existing soil microbiota are still uncertain. This study investigates the impact of arbuscular mycorrhizal fungi inoculation on grapevine performance and microbiome. The study was conducted in a vineyard of Callet cultivar in Binissalem, Mallorca, Spain. Two different treatments were applied: control and inoculation with commercial mycorrhizae complex of Rhizoglomus irregulare applied to plants through irrigation.

Effect of rising atmospheric CO2 levels on grapevine yield and composition by the middle of the 21st century: what can we learn from the VineyardFACE?

Atmospheric CO2 levels have been rising continuously since the industrial revolution, affecting crop physiology, yield and quality of harvest products, and grapevine is no exception [1]. Most of previously reported studies used potted plants in controlled environments, and explored grapevine response to relatively high CO2 levels, 700 ppm or more. The vineyardFACE, established in Geisenheim in 2012, uses a free air carbon dioxide enrichment (FACE) system to simulate a moderate (ambient +20%) increase in atmospheric CO2 in a vineyard planted with cvs. Cabernet-Sauvignon and Riesling grafted on rootstock 161-49 Couderc and SO4, respectively.

The potential of some native varieties of Argentina for the production of sparkling wines. Effect of lees contact time 

Grapevine varieties from South-America, commonly known as criollas, originated because of the natural crossbreeding of grapevine varieties brought by the Spaniards. The objective of this work was to evaluate the potential of some varieties to produce sparkling wines considering the effect of lees contact time. The following varieties were used: Moscatel Rosado, Criolla Chica, Pedro Gimenez, Blanca Oval, Canelón, and the European variety Chardonnay (control), planted in the ampelographic collection of EEA Mendoza INTA (Argentina). Pilot-scale vinifications were carried out to obtain the base wines, in 20 L glass containers. The second fermentation was performed through the traditional method.

Agronomic and oenological behavior of the minority Mandón variety on two rootstocks in the D.O. Arribes

A large population of vines of the Mandón minority red variety (synonymous with Garró) has been located in old vineyards of the D.O. Arribes (Zamora and Salamanca) to conserve and recover this minority variety. The wines made with this variety are characterized by their good structure and color, interesting harmony, an excellently low pH, with high acidity, as well as complex aromas of blue fruits and a marked and expressive minerality.