terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Abstract

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries. We performed weighted gene co-expression network analysis (WGCNA) to investigate the clustering of genes from B. cinerea and other microbes. Apart from B. cinerea, the genes of the most important filamentous fungi and yeasts in our samples showed enrichment mostly in the last phase of noble rot. Among the metabolic pathways identified, we highlighted those that may have an impact on the aroma composition of wines from noble rot grapes. These components could be the basis for further studies of metabolomic interest.

Acknowledgements: This work was financed by the NRDI fund – TKP2021-NKTA-16 – “Research and development to improve sustainability and climate resilience of viticulture and oenology at the Eszterházy Károly Catholic University”

References:

  1. Otto, M., et. al. (2022). Botrytis cinerea expression profile and metabolism differs between noble and grey rot of grapes.Food Microbiology, 106, 104037., DOI: 10.1016/j.fm.2022.104037
  2. Hegyi, Á. I., et. al. (2022). Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines.Journal of Fungi, 8(4), 378., DOI: 10.3390/jof8040378

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Hegyi, Ádám István1,2; Otto, Margot1; Hegyi-Kaló, Júlia1; Cels, Thomas1; Gomba-Tóth, Adrienn1; Golen, Richárd1; Geml, József1,3; Váczy, Kálmán Zoltán1

1 Eszterházy Károly Catholic University, Food and Wine Research Institute 3300 Eger Eszterházy sq. 1. HUNGARY
2 Doctoral School of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HUNGARY
3 ELKH-EKKE Lendület Environmental Microbiome Research Group, Eszterházy Károly Catholic University, 3300 Eger, HUNGARY

Contact the author*

Keywords

transcriptomics, noble rot, bioinformatics, omics, wine aroma, microbiome

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Mycorrhizal symbiosis modulates flavonoid and amino acid profiles in grapes of Tempranillo and Cabernet Sauvignon 

Arbuscular mycorrhizal fungi (AMF) symbiosis is probably the most widespread beneficial interaction between plants and microorganisms. AMF has been widely reported to promote grapevine growth, water and nutrient uptake as well as both biotic and abiotic stress tolerance[1]. However, the impact of AMF on grape composition has been less studied. The aim of this work was to evaluate the effects of the association between two commercial grapevine cultivars (Tempranillo and Cabernet Sauvignon grafted onto 110 rootstock) and AMF on the anthocyanin, flavonol and amino acid concentrations and profiles of grapes.

Genetic prospecting of rainfed viticulture in the region with the largest cultivated area in Chile

The Maule region hosts up to a third of the total area of vineyards in Chile, in an environment where ancient practices inherited from the colonial past coexist with modernity and dynamism that include technified irrigation and fine vines. In the dry land of Maule there is a viticulture that has subsisted with ancient vines and traditions transmitted over generations, and there is little clarity about the origin and classification of the Maule viticulture, giving rise to the use of different concepts as synonyms to describe the ancient, minority, patrimonial or Criollas vines. In order to characterize and protect the ancient material, we studied the genetic diversity of a territorial collection that covers 80% of the communes of the region, prioritizing plants established more than 40-60 years ago.

Genetic study of wild grapevines in La Rioja region

Since the mid-1980s, several surveys have been carried out in La Rioja to search for populations of the sylvestris grapevine subspecies (Vitis vinifera L. subsp. sylvestris Gmelin). The banks of the Ebro River and its tributaries (Alhama, Cidacos, Leza, Iregua, Najerilla, Oja and Tirón rivers), as well as the surrounding vegetation of their valleys have been covered. So far, all the populations found are alluvial, forming part of the riparian vegetation of the Najerilla (the first reported population in La Rioja [1]), Iregua, and the vicinity of Oja valleys.

Glucosidase and esterase salivary activities and their involvement in consumer’s wine sensory perception and liking

Wine flavour is the integration of distinct physiologically defined sensory systems that combine taste, aroma and trigeminal sensations, and it is a key determinant factor for the acceptance of wine by consumers. Volatile compounds, are important contributors to wine flavour, specially to aroma. These small and low-boiling point compounds are easily released into the air allowing to enter and move within the nasal or oral cavities where they can bind the olfactory receptors. Additionally, wine also contains aroma precursors, which are non-volatile compounds, but that can be broken down releasing volatile odorants. During wine tasting, all these chemicals (volatiles and non-volatiles) can be submitted to the action of salivary enzymes.