terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Abstract

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries. We performed weighted gene co-expression network analysis (WGCNA) to investigate the clustering of genes from B. cinerea and other microbes. Apart from B. cinerea, the genes of the most important filamentous fungi and yeasts in our samples showed enrichment mostly in the last phase of noble rot. Among the metabolic pathways identified, we highlighted those that may have an impact on the aroma composition of wines from noble rot grapes. These components could be the basis for further studies of metabolomic interest.

Acknowledgements: This work was financed by the NRDI fund – TKP2021-NKTA-16 – “Research and development to improve sustainability and climate resilience of viticulture and oenology at the Eszterházy Károly Catholic University”

References:

  1. Otto, M., et. al. (2022). Botrytis cinerea expression profile and metabolism differs between noble and grey rot of grapes.Food Microbiology, 106, 104037., DOI: 10.1016/j.fm.2022.104037
  2. Hegyi, Á. I., et. al. (2022). Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines.Journal of Fungi, 8(4), 378., DOI: 10.3390/jof8040378

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Hegyi, Ádám István1,2; Otto, Margot1; Hegyi-Kaló, Júlia1; Cels, Thomas1; Gomba-Tóth, Adrienn1; Golen, Richárd1; Geml, József1,3; Váczy, Kálmán Zoltán1

1 Eszterházy Károly Catholic University, Food and Wine Research Institute 3300 Eger Eszterházy sq. 1. HUNGARY
2 Doctoral School of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HUNGARY
3 ELKH-EKKE Lendület Environmental Microbiome Research Group, Eszterházy Károly Catholic University, 3300 Eger, HUNGARY

Contact the author*

Keywords

transcriptomics, noble rot, bioinformatics, omics, wine aroma, microbiome

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.

Exploring the genetic diversity of leaf flavonoids content in a set of Iberian grapevine cultivars: preliminary results

The use of grapevine genetic diversity is a way to mitigate the negative impacts of climate change on viticulture systems. Leaf epidermal flavonoids (including flavonols and anthocyanins) are involved in plant defense mechanisms against environmental stresses, like high temperatures or excessive solar radiation [1,2]. Among other factors, they modulate light absorption, which reduces photoinhibition processes in photosynthetic tissues [1]. Therefore, the identification of grapevine cultivars with an increased content on leaf epidermal flavonoids arises as a potential avenue to improve grapevine tolerance to some detrimental environmental stresses.

Grapevine adaptation to drought and resistance to Neofusicoccum parvum, causal agent of Botryosphaeria dieback

The sustainability of viticulture in response to climate change has been addressed mainly considering agronomic impacts, such as water management and diseases, either separately or together.
In grapevines, there is strong evidence that different genotypes respond differently to biotic and abiotic stresses. A screening was conducted on various local cultivars in response to drought and Neofusicoum parvum infection aiming to evaluate their susceptibility to abiotic stress and resistance to fungal diseases.

Long-lasting flavour perception of wines treated with oenological additives considering the individual PROP taste-phenotype

The use of oenological additives is becoming a common practice due to the technological and sensory properties they provide to the wines. However, the number of studies focused on the impact that these additives might induce on wine flavor perception during wine tasting is still quite scarce. The aim of this work was to evaluate the effect of three different types of common oenological additives: two oenotannins (ellagitannin and gallotannin) and a commercial preparation of yeast mannoproteins on the long-lasting flavor perception (aroma and astringency).

Influence of polysaccharide extracts from wine by-products on the volatile composition of sparkling white wines

In the production of sparkling wines, during the second fermentation, mannoproteins are released by yeast autolysis, which affect the quality of the wines. The effect of mannoproteins has been extensively studied, and may affect aroma and foam quality. However, there are no studies on the effect of other polysaccharides such as those from grapes. Considering the large production of waste from the wine industry, it was proposed to obtain polysaccharide-rich extracts from some of these by-products[1].