terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Abstract

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries. We performed weighted gene co-expression network analysis (WGCNA) to investigate the clustering of genes from B. cinerea and other microbes. Apart from B. cinerea, the genes of the most important filamentous fungi and yeasts in our samples showed enrichment mostly in the last phase of noble rot. Among the metabolic pathways identified, we highlighted those that may have an impact on the aroma composition of wines from noble rot grapes. These components could be the basis for further studies of metabolomic interest.

Acknowledgements: This work was financed by the NRDI fund – TKP2021-NKTA-16 – “Research and development to improve sustainability and climate resilience of viticulture and oenology at the Eszterházy Károly Catholic University”

References:

  1. Otto, M., et. al. (2022). Botrytis cinerea expression profile and metabolism differs between noble and grey rot of grapes.Food Microbiology, 106, 104037., DOI: 10.1016/j.fm.2022.104037
  2. Hegyi, Á. I., et. al. (2022). Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines.Journal of Fungi, 8(4), 378., DOI: 10.3390/jof8040378

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Hegyi, Ádám István1,2; Otto, Margot1; Hegyi-Kaló, Júlia1; Cels, Thomas1; Gomba-Tóth, Adrienn1; Golen, Richárd1; Geml, József1,3; Váczy, Kálmán Zoltán1

1 Eszterházy Károly Catholic University, Food and Wine Research Institute 3300 Eger Eszterházy sq. 1. HUNGARY
2 Doctoral School of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HUNGARY
3 ELKH-EKKE Lendület Environmental Microbiome Research Group, Eszterházy Károly Catholic University, 3300 Eger, HUNGARY

Contact the author*

Keywords

transcriptomics, noble rot, bioinformatics, omics, wine aroma, microbiome

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Biodiversity and biocontrol ability of Trichoderma natural populations in soil vineyards from Castilla y León region (Spain)

Trichoderma is a microorganism present in many agricultural soils and some of its species could be used as natural biological control agents. In this work, the presence of natural populations of Trichoderma was estimated in soil vineyard and its biocontrol capacity against Phaeoacremonium minimum, one of the main agent causals of grapevine trunk diseases instead of using pesticides. Moreover, physicochemical variables in soil such as pH, organic matter and nutrients were evaluated to determine a possible correlation to natural populations of Trichoderma.

Water and nutritional savings shape non-structural carbohydrates in grapevine (Vitis vinifera L.) cuttings

Global changes and sustainability challenge researchers in saving water and nutrients. The response of woody crops, which can be forced at facing more drought events during their life, is particularly important. Vitis vinifera can be an important model for its relevance in countries subjected to climate changes and its breeding, requiring cuttings plantation and strong pruning.

Impact of climate on berry weight dynamics of a wide range of Vitis vinifera cultivars 

In order to study the impact of climate change on Bordeaux grape varieties and to assess the behavior of candidate grape varieties potentially better adapted to the new climatic conditions, an experimental vineyard composed of 52 grape varieties was planted in 2009 at the INRAE Bordeaux Aquitaine center[1]. Among the many parameters studied since 2012, berry weight for each variety was measured weekly from mid-veraison to maturity, with four independent replicates. The kinetics obtained allowed to study berry growth, a key parameter in grape composition and yield.

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements.

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines.