terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Abstract

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries. We performed weighted gene co-expression network analysis (WGCNA) to investigate the clustering of genes from B. cinerea and other microbes. Apart from B. cinerea, the genes of the most important filamentous fungi and yeasts in our samples showed enrichment mostly in the last phase of noble rot. Among the metabolic pathways identified, we highlighted those that may have an impact on the aroma composition of wines from noble rot grapes. These components could be the basis for further studies of metabolomic interest.

Acknowledgements: This work was financed by the NRDI fund – TKP2021-NKTA-16 – “Research and development to improve sustainability and climate resilience of viticulture and oenology at the Eszterházy Károly Catholic University”

References:

  1. Otto, M., et. al. (2022). Botrytis cinerea expression profile and metabolism differs between noble and grey rot of grapes.Food Microbiology, 106, 104037., DOI: 10.1016/j.fm.2022.104037
  2. Hegyi, Á. I., et. al. (2022). Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines.Journal of Fungi, 8(4), 378., DOI: 10.3390/jof8040378

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Hegyi, Ádám István1,2; Otto, Margot1; Hegyi-Kaló, Júlia1; Cels, Thomas1; Gomba-Tóth, Adrienn1; Golen, Richárd1; Geml, József1,3; Váczy, Kálmán Zoltán1

1 Eszterházy Károly Catholic University, Food and Wine Research Institute 3300 Eger Eszterházy sq. 1. HUNGARY
2 Doctoral School of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HUNGARY
3 ELKH-EKKE Lendület Environmental Microbiome Research Group, Eszterházy Károly Catholic University, 3300 Eger, HUNGARY

Contact the author*

Keywords

transcriptomics, noble rot, bioinformatics, omics, wine aroma, microbiome

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Physicochemical behaviour of wine spirit and wine distillate aged in Sherry Casks® and Brandy casks

Brandy is a spirit drink made from “wine spirit” (<86% Alcohol by Volume – ABV; high levels of congeners and they are mainly less volatile than ethanol), it may be blended with a “wine distillate” (<94.8%ABV; low levels of congeners and these are mainly more volatile than ethanol), as long as that distillate does not exceed a maximum of 50% of the alcoholic content of the finished product[1]. Brandy must be aged for at least 6 months in oak casks with <1000L of capacity. During ageing, changes occur in colour, flavour, and aroma that improve the quality of the original distillate.

Long-lasting flavour perception of wines treated with oenological additives considering the individual PROP taste-phenotype

The use of oenological additives is becoming a common practice due to the technological and sensory properties they provide to the wines. However, the number of studies focused on the impact that these additives might induce on wine flavor perception during wine tasting is still quite scarce. The aim of this work was to evaluate the effect of three different types of common oenological additives: two oenotannins (ellagitannin and gallotannin) and a commercial preparation of yeast mannoproteins on the long-lasting flavor perception (aroma and astringency).

The tolerance of grapevine rootstocks to water deficit is related to root morphology and xylem anatomy traits 

Climate change is altering water balances, thereby compromising water availability for crops. In grapevine, the strategic selection of genotypes more tolerant to soil water deficit can improve the resilience of the vineyard under this scenario. Previous studies demonstrated that root anatomical and morphological traits determine vine performance under water deficit conditions. Therefore, 13 ungrafted rootstock genotypes, 6 commercial (420 A, 41 B, Evex 13-5, Fercal, 140 Ru y 110 R), and 7 from new breeding programs (RG2, RG3, RG4, RG7, RG8, RG9 and RM2) were evaluated in pots during 2021 and 2022.

Polysaccharide families of lyophilized extracts obtained from unfermented varietal grape pomaces

The recovery of bioactive compounds from grape and wine by-products is currently an important objective for revaluation and sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds. The aim of this study was to evaluate the polysaccharide (PS) composition of extracts obtained from pomaces of different white and red grape varieties of Castilla y León. Grape pomaces were obtained after the pressing in the winemaking process.

Biotic and abiotic factors affecting physiological aspects underlying vegetative vigour in two commercial grapevine varieties

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.