terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Abstract

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.

We currently investigate molecularly imprinted polymers (MIPs) as a dedicated tool to efficiently extract polyphenols from GSE with high dosage, controlled composition and improved bioavailability. The materials will be tailored such that either a selection of already known and potent polyphenols will be extracted, or more generically, that the majority of GSE containing polyphenols will be extracted in a rather untargeted approach. The same MIPs based on biodegradeable polymers will be used as innovative pharmaceutical formulations / drug delivery matrices packaging the polyphenols extracted from grape by-products, which serve as a resource of bioactive compounds with the distinct circular economic effect of reducing winemaking environmental impact.

Acknowledgements: We would like to thank the International Ambition Pack from La Région Auvergne-Rhône-Alpes for support of this project.

1)  A. Molinelli et al., Advanced Solid Phase Extraction Using Molecularly Imprinted Polymers for the Determination of Quercetin in Red Wine, Journal of Agricultural and Food Chemistry, 50 (7), 1804–1808 (2002), DOI: 10.1021/jf011213q

2)  S. Rajpal et al., An in silico predictive method to select multimonomer combinations for peptide imprinting, J. Mater. Chem. B 10, 6618-6626 (2022), DOI: https://doi.org/10.1039/D2TB00418F

3)  A. Kotyrba et al., Development of Silica Nanoparticle Supported Imprinted Polymers for Selective Lysozyme Recognition, Nanomaterials 11(12), 3287 (2021), DOI: https://doi.org/10.3390/nano11123287

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Boris Mizaikoff1,2*, Anika Kotyrba1, Mélina Begou2

1Ulm University, Institute of Analytical and Bioanalytical Chemistry, Ulm, Germany
2Hahn-Schickard, Ulm, Germany
3Université Clermont Auvergne, Department of Pharmacology, Clermont, France

Contact the author*

Keywords

molecularly imprinted polymers, polyphenols, grape seed extract, multiple sclerosis

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].

Effects of different soil types and soil management on greenhouse gas emissions 

Soil is important in the carbon cycle and the dynamics of greenhouse gases (CO2, CH4 and N2O). Key soil characteristics, such as organic matter content, texture, structure, pH and microbial activity, play a determining role in GHG emissions[1]. The objective of the study is to delimit different types of soil, with different soil management and to be able to verify the differences in CO2, CH4 and N2O emissions. The study was carried out in a vineyard of Bodegas Campo Viejo in Logroño (La Rioja), whose plant material is Vitis vinifera L. cv. Tempranillo.

Phenolic extraction and dissolved oxygen concentration during red wines fermentations with Airmixig M.I.™

During red wine fermentation, the extraction of phenolics compounds and sufficient oxygen provision are critical for wine quality [1,2]. In this trial, we aimed at evaluating the kinetics of phenolic extraction and dissolved oxygen during red wine fermentations using the airmixing system. Twenty lots of red grape musts were fermented in 300.000 L tanks, equipped with airmixing, using two injection regimes (i.e., high and low intensity, and high and low daily frequency). An oxygen analyzer was introduced into the tanks in order to record the concentration of dissolved oxygen over time.

Culturable microbial communities associated with the grapevine soil in vineyards of La Rioja, Spain

The definition of soil health is complex due to the lack of agreement on adequate indicators and to the high variability of global soils. Nevertheless, it has been widely used as synonymous of soil quality for more than one decade, and there is a consensus warning of scientists that soil quality and biodiversity loss are occurring due to the traditional intensive agricultural practices.
In this work we monitored a set of soil parameters, both physicochemical and microbiological, in an experimental vineyard under three different management and land use systems: a) addition of external organic matter (EOM) to tilled soil; b) no tillage and plant cover between grapevine rows, and c) grapevines planted in rows running down the slope and tilled soil.

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.