terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Abstract

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.

We currently investigate molecularly imprinted polymers (MIPs) as a dedicated tool to efficiently extract polyphenols from GSE with high dosage, controlled composition and improved bioavailability. The materials will be tailored such that either a selection of already known and potent polyphenols will be extracted, or more generically, that the majority of GSE containing polyphenols will be extracted in a rather untargeted approach. The same MIPs based on biodegradeable polymers will be used as innovative pharmaceutical formulations / drug delivery matrices packaging the polyphenols extracted from grape by-products, which serve as a resource of bioactive compounds with the distinct circular economic effect of reducing winemaking environmental impact.

Acknowledgements: We would like to thank the International Ambition Pack from La Région Auvergne-Rhône-Alpes for support of this project.

1)  A. Molinelli et al., Advanced Solid Phase Extraction Using Molecularly Imprinted Polymers for the Determination of Quercetin in Red Wine, Journal of Agricultural and Food Chemistry, 50 (7), 1804–1808 (2002), DOI: 10.1021/jf011213q

2)  S. Rajpal et al., An in silico predictive method to select multimonomer combinations for peptide imprinting, J. Mater. Chem. B 10, 6618-6626 (2022), DOI: https://doi.org/10.1039/D2TB00418F

3)  A. Kotyrba et al., Development of Silica Nanoparticle Supported Imprinted Polymers for Selective Lysozyme Recognition, Nanomaterials 11(12), 3287 (2021), DOI: https://doi.org/10.3390/nano11123287

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Boris Mizaikoff1,2*, Anika Kotyrba1, Mélina Begou2

1Ulm University, Institute of Analytical and Bioanalytical Chemistry, Ulm, Germany
2Hahn-Schickard, Ulm, Germany
3Université Clermont Auvergne, Department of Pharmacology, Clermont, France

Contact the author*

Keywords

molecularly imprinted polymers, polyphenols, grape seed extract, multiple sclerosis

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Sparkling wines and atypical aging: investigating the risk of refermentation

Sparkling wine (SW) production entails a two-steps process where grape must undergoes a primary fermentation to produce a base wine (BW) which is then refermented to become a SW. This process allows for the development of a new physicochemical profile characterized by the presence of foam and a different organoleptic profile.

Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Ultra High-Pressure Homogenization (UHPH) is a high-pressure pumping at 300 MPa (>200 MPa) with a subsequent depressurization against a highly resistant valve made of tungsten carbide covered by ceramic materials or carbon nanoparticles. The intense impact and shear efforts produce the nano-fragmentation of colloidal biopolymers including the elimination of microorganism (pasteurization or sterilization depending on in-valve temperature) and the inactivation of enzymes.

Ecophysiological characterisation of terroir effects on Vitis vinifera L. Chardonnay and pinot noir in south african cool climate regions

Terroir encompasses environmental (climate, geology, soil and topography), genetic (cultivar and clone) and human factors (oenological and viticultural practices). Climate change brings about shifts in the suitability of a region for the growth of specific grapevine cultivars. This study focused on climatic and fruit parameters (berry size, weight, pH, total acidity (TA) and phenolics) to characterise the terroir effect in Vitis vinifera L. cultivars Chardonnay and Pinot Noir vineyards in the Cape South Coast region (Walker Bay and Elgin).

Analysis of the interaction of melatonin with glycolytic proteins in Saccharomyces cerevisiae during alcoholic fermentation 

Melatonin is a bioactive compound with antioxidant properties, that has been found in many fermented beverages, such as beer and wine [1]. Indeed, it has been shown that yeast can synthesize melatonin during alcoholic fermentation, although its role inside the cell, as well as the metabolic pathway involved in its synthesis, is still unclear [1]. Recent studies showed that during fermentation, melatonin interacts with different proteins of the glycolytic pathway in both Saccharomyces and non-Saccharomyces yeast, for instance glyceraldehyde 3-phosphate dehydrogenase, pyruvate kinase or enolase [2].

Effects of laccase from Botrytis cinerea on the oxidative degradation kinetics of the five natural grape anthocyanins

Enzymatic browning[1] is an oxidation process that occurs in many foods that increases the brown colour[2]. This problem is especially harmful in the wine industry[3]. especially when the grapes are infected by grey rot since this fung release the oxidative enzyme laccase[4]. In the particular case of red wines, the presence of laccase implies the deterioration of the red colour and can even cause the precipitation of the coloring matter (oxidasic haze)[5].