terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Abstract

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.

We currently investigate molecularly imprinted polymers (MIPs) as a dedicated tool to efficiently extract polyphenols from GSE with high dosage, controlled composition and improved bioavailability. The materials will be tailored such that either a selection of already known and potent polyphenols will be extracted, or more generically, that the majority of GSE containing polyphenols will be extracted in a rather untargeted approach. The same MIPs based on biodegradeable polymers will be used as innovative pharmaceutical formulations / drug delivery matrices packaging the polyphenols extracted from grape by-products, which serve as a resource of bioactive compounds with the distinct circular economic effect of reducing winemaking environmental impact.

Acknowledgements: We would like to thank the International Ambition Pack from La Région Auvergne-Rhône-Alpes for support of this project.

1)  A. Molinelli et al., Advanced Solid Phase Extraction Using Molecularly Imprinted Polymers for the Determination of Quercetin in Red Wine, Journal of Agricultural and Food Chemistry, 50 (7), 1804–1808 (2002), DOI: 10.1021/jf011213q

2)  S. Rajpal et al., An in silico predictive method to select multimonomer combinations for peptide imprinting, J. Mater. Chem. B 10, 6618-6626 (2022), DOI: https://doi.org/10.1039/D2TB00418F

3)  A. Kotyrba et al., Development of Silica Nanoparticle Supported Imprinted Polymers for Selective Lysozyme Recognition, Nanomaterials 11(12), 3287 (2021), DOI: https://doi.org/10.3390/nano11123287

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Boris Mizaikoff1,2*, Anika Kotyrba1, Mélina Begou2

1Ulm University, Institute of Analytical and Bioanalytical Chemistry, Ulm, Germany
2Hahn-Schickard, Ulm, Germany
3Université Clermont Auvergne, Department of Pharmacology, Clermont, France

Contact the author*

Keywords

molecularly imprinted polymers, polyphenols, grape seed extract, multiple sclerosis

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effects of different soil types and soil management on greenhouse gas emissions 

Soil is important in the carbon cycle and the dynamics of greenhouse gases (CO2, CH4 and N2O). Key soil characteristics, such as organic matter content, texture, structure, pH and microbial activity, play a determining role in GHG emissions[1]. The objective of the study is to delimit different types of soil, with different soil management and to be able to verify the differences in CO2, CH4 and N2O emissions. The study was carried out in a vineyard of Bodegas Campo Viejo in Logroño (La Rioja), whose plant material is Vitis vinifera L. cv. Tempranillo.

Grapevine cane pruning extract enhances plant physiological capacities and decreases phenolic accumulation in canes and leaves 

Vine cane extracts are a valuable byproduct due to their rich content of polyphenols, vitamins, and other beneficial compounds, which can affect and benefit the vine and the grapes. This study aims to evaluate the response of grapevine plants to irrigation with water supplemented with a vine cane extract, both at physiology response and phenolic composition in different parts of the plant (root, trunk, shoot, leaf, and berry).
Cane extract was obtained by macerating crushed pruning residues with warm water (5:1) and pectolytic enzymes. Two-year-old potted plants were irrigated with water (Control) while others were irrigated with cane extracts, either at 1:4 (w/v, cane extract/water; T 1:4) or at 1:8 (w/v, cane extract/water; T 1:8).

Prediction of aromatic attributes of red wines from its colour properties 

Wine perception is a multisensory experience that makes use of the sight, smell, and taste senses. When wine is sensorially assessed, the stimulus received generates multiple signals that tasters convert into organoleptic descriptors. Colour is commonly the first attribute evaluated during wine tasting. Moreover, the colour properties provide the taster with a priori information of the wine’s aroma. This preconceived perception is later confirmed or denied during the aroma evaluation.

Correlative study between degradation of rosé wine under accelerated conditions and under normal conditions

Several studies have tried to develop different methods to study the photodegradation of wine in an accelerated way, trying to elucidate the effect of light on the wine compounds[1]. In a previous study, our team developed a chamber that speeds up the photodegradation of rosé wine[2]. In the present work we have tried to establish a correlation between irradiation times in accelerated conditions and the natural exposure to the cycles of light that usually exist in markets or at home.

Effect of soil particle size on vine water status, leaf ABA content and berry quality in Nebbiolo grapes

The root and shoot abscisic acid (ABA) accumulation in response to water deficit and its relation with stomatal conductance is longtime known in grapevine. ABA-dependent and ABA-independent signalling response to osmotic stress coexist in sessile plants. In grapevine, the signaling role of ABA in response to water stress conditions and its influence on berry quality is critical to manage grapevine acclimation to climate change.