terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Abstract

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution. This work focuses on the identification of wine intake biomarkers in 24-h urine samples of free-living volunteers using untargeted metabolomics approach. Two groups were included: (i) volunteers with daily and moderate wine consumption, and (ii) control group, volunteers who never drink wine. Urine samples (24-h) were analysed by liquid chromatography coupled with high-resolution mass spectrometry (UPLC-QToF), using two stationary phases (RP and HILIC) to separate metabolites of different polarities, moreover all the analyses were done in both positive and negative ionization modes. The most significant compounds highlighted after performing an OPLS-DA were tentatively identified based on their accurate masses and spectra information. Different metabolites associated with wine intake have been tentatively proposed, such as aminoacids and peptides, and different phenol metabolites.

Acknowledgements: MCIN / AEI /10.13039/501100011033 and the European Union NextGenerationEU/PRTR through the project PID2019-108851RB-C22. M.J-S. thanks University of La Rioja for her PostDoc grant financed by the European Union-NextGenerationEU.

References:

  1. Hrelia S. et al. (2023) Moderate Wine Consumption and Health: A Narrative Review. Nutrients, 15: 175-200, DOI 10.3390/nu15010175.

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Jiménez-Salcedo M.1,2*, Manzano JI.1, Pérez-Matute, P.3, Motilva MJ. 1

1 Instituto de Ciencias de la Vid y el Vino-ICVV (CSIC, UR, GR) 26007 Logroño (España)
2 Universidad de La Rioja, 26006 Logroño (España)
3 Infectious Diseases, Microbiota and Metabolism Unit, Center for Biomedical Research of La Rioja (CIBIR), CSIC Associated Unit. 26006 Logroño (España)

Contact the author*

Keywords

untargeted metabolomics, wine, biomarker, polyphenols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Vines are exposed to environmental conditions that cause abiotic stress on the plants (drought, nutrient and mineral deficits, salinity, etc.). Polyamines are growth regulators involved in various physiological processes, as in abiotic plant stress responses. Stressful conditions can modify grape’s composition, and in this work, we have focused on studying the effect of abiotic stress on the composition of polyamines and amino acids in grapes. In addition, the effect of grape variety on these compounds has been studied.

New oenological criteria for selecting strains of Lachancea thermotolerans for wine technology

The study conducted various fermentations of different grape juices using various strains of Lachancea thermotolerans and one strain of Saccharomyces cerevisiae. Because of the new conditions caused by climate change, wine acidity must be influenced as well as the volatile profile. Non-Saccharomyces yeasts such as L. thermotolerans are real options to mitigate the impact of climate change in wine production.

Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Grapevine rootstocks provide protection against environmental biotic and abiotic stresses. Nitrogen (N) and iron (Fe) are growth-limiting factors in many crop plants due to their effects on the chlorophyll and photosynthetic characteristics. Iron nutrition of plants can be significantly affected by different nitrogen forms through altering the uptake ratio of cations and anions, and changing rhizosphere pH. The aim of this study was to investigate the response mechanisms of grapevine rootstocks due to the interaction between different nitrogen forms and iron uptake.

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.