terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Abstract

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution. This work focuses on the identification of wine intake biomarkers in 24-h urine samples of free-living volunteers using untargeted metabolomics approach. Two groups were included: (i) volunteers with daily and moderate wine consumption, and (ii) control group, volunteers who never drink wine. Urine samples (24-h) were analysed by liquid chromatography coupled with high-resolution mass spectrometry (UPLC-QToF), using two stationary phases (RP and HILIC) to separate metabolites of different polarities, moreover all the analyses were done in both positive and negative ionization modes. The most significant compounds highlighted after performing an OPLS-DA were tentatively identified based on their accurate masses and spectra information. Different metabolites associated with wine intake have been tentatively proposed, such as aminoacids and peptides, and different phenol metabolites.

Acknowledgements: MCIN / AEI /10.13039/501100011033 and the European Union NextGenerationEU/PRTR through the project PID2019-108851RB-C22. M.J-S. thanks University of La Rioja for her PostDoc grant financed by the European Union-NextGenerationEU.

References:

  1. Hrelia S. et al. (2023) Moderate Wine Consumption and Health: A Narrative Review. Nutrients, 15: 175-200, DOI 10.3390/nu15010175.

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Jiménez-Salcedo M.1,2*, Manzano JI.1, Pérez-Matute, P.3, Motilva MJ. 1

1 Instituto de Ciencias de la Vid y el Vino-ICVV (CSIC, UR, GR) 26007 Logroño (España)
2 Universidad de La Rioja, 26006 Logroño (España)
3 Infectious Diseases, Microbiota and Metabolism Unit, Center for Biomedical Research of La Rioja (CIBIR), CSIC Associated Unit. 26006 Logroño (España)

Contact the author*

Keywords

untargeted metabolomics, wine, biomarker, polyphenols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The generation of suspended cell wall material may limit the effect of ultrasound in some varieties

The disruptive effect exerted by high-power ultrasound (US) on plant cell walls, natural barriers to the diffusion of compounds of interest during the maceration of red wines, is established as the reason behind the chromatic improvement that its treatment causes. However, sometimes this improvement is not observed, especially with short maceration times. The presence of a high quantity of suspended cell wall material, which formation is favored by the sonication, could be the cause of this lack of positive results since this cell wall material has a high affinity for phenolic compounds.

Polysaccharide families of lyophilized extracts obtained from unfermented varietal grape pomaces

The recovery of bioactive compounds from grape and wine by-products is currently an important objective for revaluation and sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds. The aim of this study was to evaluate the polysaccharide (PS) composition of extracts obtained from pomaces of different white and red grape varieties of Castilla y León. Grape pomaces were obtained after the pressing in the winemaking process.

Perception, liking and emotional response of tropical fruit aromas in Chardonnay wines

Tropical fruit aromas in wines are thought to be important to wine consumers, although there is little research to confirm this statement. With so many wine styles available, it has become important to understand the qualities that are desirable to consumers and how to achieve those qualities. Thiols and esters are compounds that have been found to cause tropical fruit aromas in chardonnay (ref). Fermentation temperature gradients and skin contact were found to increase these compounds using micro scale fermentations. This work aimed to scale up these fermentations/operations to determine if the desired tropical fruit aromas could still be achieved and if there is a perceivable difference in tropical fruit aromas, liking, and emotional response in the wines at the consumer level.

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Physicochemical behaviour of wine spirit and wine distillate aged in Sherry Casks® and Brandy casks

Brandy is a spirit drink made from “wine spirit” (<86% Alcohol by Volume – ABV; high levels of congeners and they are mainly less volatile than ethanol), it may be blended with a “wine distillate” (<94.8%ABV; low levels of congeners and these are mainly more volatile than ethanol), as long as that distillate does not exceed a maximum of 50% of the alcoholic content of the finished product[1]. Brandy must be aged for at least 6 months in oak casks with <1000L of capacity. During ageing, changes occur in colour, flavour, and aroma that improve the quality of the original distillate.