terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Abstract

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution. This work focuses on the identification of wine intake biomarkers in 24-h urine samples of free-living volunteers using untargeted metabolomics approach. Two groups were included: (i) volunteers with daily and moderate wine consumption, and (ii) control group, volunteers who never drink wine. Urine samples (24-h) were analysed by liquid chromatography coupled with high-resolution mass spectrometry (UPLC-QToF), using two stationary phases (RP and HILIC) to separate metabolites of different polarities, moreover all the analyses were done in both positive and negative ionization modes. The most significant compounds highlighted after performing an OPLS-DA were tentatively identified based on their accurate masses and spectra information. Different metabolites associated with wine intake have been tentatively proposed, such as aminoacids and peptides, and different phenol metabolites.

Acknowledgements: MCIN / AEI /10.13039/501100011033 and the European Union NextGenerationEU/PRTR through the project PID2019-108851RB-C22. M.J-S. thanks University of La Rioja for her PostDoc grant financed by the European Union-NextGenerationEU.

References:

  1. Hrelia S. et al. (2023) Moderate Wine Consumption and Health: A Narrative Review. Nutrients, 15: 175-200, DOI 10.3390/nu15010175.

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Jiménez-Salcedo M.1,2*, Manzano JI.1, Pérez-Matute, P.3, Motilva MJ. 1

1 Instituto de Ciencias de la Vid y el Vino-ICVV (CSIC, UR, GR) 26007 Logroño (España)
2 Universidad de La Rioja, 26006 Logroño (España)
3 Infectious Diseases, Microbiota and Metabolism Unit, Center for Biomedical Research of La Rioja (CIBIR), CSIC Associated Unit. 26006 Logroño (España)

Contact the author*

Keywords

untargeted metabolomics, wine, biomarker, polyphenols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Drought is one of the main challenges for viticulture in the context of global change. The choice of rootstock could be leveraged for vineyard adaptation to drought as we can improve plant performance without modifying the scion variety. However, most of the existing rootstocks, selected over a century ago, have a narrow genetic background which could compromise their adaptive potential.

The weak role of organic mulches in shaping bacterial communities in grapevine

The interest in sustainable and ecologic agricultural practices in grapevine has grown significantly in recent years in the context of ecological transition. Organic mulches are treatments that support the circular economy and positively affect the soil and the plant. They are an alternative to herbicides and other conventional practices since they may influence soil moisture, erosion, structure and weed control. However, their effects on the soil and must microbiota remain unknown.

Physico-chemical properties of vine pruning residues with potential as enological additive

Grapes are one of the world’s primary fruit crops, and pruning activities generate high amounts of annual wood wastes [1]. These pruning shoots contain valuable phenolic compounds and could have numerous potential applications [1,2]. Consequently, the aim of this work was to evaluate the physico-chemical properties of vine pruning residues with potential as enological additives. For this purpose, grapevine shoots from 12 varieties grown in Chile were collected during the winter of 2021.

Irrigation frequency in four grapevine red varieties in Spain. Effect on must volatile composition

The irrigation water management in the vineyard is a crucial aspect to obtain sustainable quality production over time. Previous studies have set the water requirements to be applied in the vineyard at 30 % of the reference evapotranspiration (ET0), although there are no studies that settle the effects of the frequency of irrigation application on red varieties in Spain. The present study contemplates the application of deficit irrigation (30 % ET0) applying a weekly dose in a single irrigation (T07) or in two irrigation events (T03) per week. The study has been carried out in 2021-2022 with four red varieties in different Spanish wine regions: Garnacha Tinta (Badajoz), Tempranillo (Valladolid), Syrah (Albacete) and Mencía (Lugo). The effects of irrigation frequency on must volatile composition have been evaluated through GC-MS.

Plastic debris at vines: carriers of pollutants in the environment?

Modern agriculture employs large amounts of plastics, such as mulching and greenhouse films, thermal covers, plant protection tubes and tying tape. The latter two types are very common in viticulture. Guard tubes are employed to protect young vines from mechanic and atmospheric damage, whilst polymeric tying tape has replaced natural-origin materials to hold the canopy of vines. Both materials are made on synthetic polymers, which include a range of additives to improve their environmental stability remaining in the environment of vineyards for years. During this time, they are exposed to the range of pesticides (fungicides, insecticides and in a lesser extend herbicides) applied to vines.