terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Abstract

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities. Grapevine leaves being a disposable byproduct in viticulture, their potential valorization as a source of polyphenols is a topic of interest.

Our objective was to compare the diversity of grapevine leaves composition by screening qualitatively and quantitatively the polyphenol content in leaves of 50 cultivars grown in the same field of an experimental collection at Bordeaux INRAe. Fresh leaves were collected at the same period in summer, freeze-dried, ground to a fine powder and polyphenols were extracted twice with combination of organic solvents (100% and 70% methanol). A targeted HPLC-MS/MS approach was used for the quantification with available standards of 60 different polyphenols.

The results showed high variability in polyphenols content. Nevertheless, caftaric acid and quercetin 3-glucuronide were the major compounds detected in all leaves. Flavanols, and more particularly the ratio catechin/epicatechin, could be explored as markers to determine leaf cultivar, ranging from 0.5 to 42. Stilbenes were minor compounds in all leaves, found mainly in the forms of trans- and cis-piceid. Minor presence of resveratrol and its oligomers was assessed by HPLC-HRMS/MS.

Acknowledgements: The authors wish to thank the UE Viticole and Louis Bordenave for management of the experimental vineyard and the support of Bordeaux Metabolome

 

References:

(1) Lemaitre-Guillier et al (2021) VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine. https://doi.org/10.3390/molecules26144258

(2) Goufo et al (2020). A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves. doi: 10.3390/antiox9050398

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alan Jamain1, Margot Larose1, Andreu Mairata2, Manon Delapena1, Antonio Palos-Pinto1, Céline Franc1, Maria Lafargue3, Ghislaine Hilbert-Masson3, Stéphanie Cluzet1, Josep Valls Fonayet1

1Enology, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France
2Instituto de Ciencias de la Vid y el Vino, 26007 Logroño (La Rioja) ESPAÑA
3EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France

Contact the author*

Keywords

Vitis vinifera, Quercetin, Caftaric, Byproducts

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Cumulative effect of deficit irrigation and salinity on vine responses

Climate change is increasing water needs in most of the wine growing regions while reducing the availability and quality of water resources for irrigation. In this context, the sustainability of Mediterranean viticulture depends on grapevine responses to the combinations of water and salt stress. With this aim, this work studies the effects of deficit irrigation and salinity on the physiology of the Tempranillo cultivar (Vitis vinifera L.) grafted onto a drought and salinity tolerant rootstock (1103 Paulsen).

Effect of foliar application of urea and nano-urea on the cell wall of Monastrell grape skins

The foliar application of urea has been shown to be able to satisfy the specific nutritional needs of the vine as well as to increase the nitrogen composition of the must. On the other hand, the use of nanotechnology could be of great interest in viticulture as it would help to slow down the release of urea and protect it against possible degradation. Several studies indicate that cell wall synthesis and remodeling are affected by nitrogen availability.

Understanding the impact of rising temperatures due to climate change on aromatic compositions in Malbec wines from Mendoza, Argentina

Mendoza is one of Argentina’s most important and outstanding wine regions producing the renowned Malbec wines due to its optimal soil and weather conditions. However, the effects of 21st-century climate change would negatively impact Malbec wines quality. This study investigated the effect of temperature increase and the impact of plant hormone abscisic acid (ABA) used to mitigate the negative effect of temperature increase on Malbec wines aromatic composition through GC-MS. Four treatments were applied on vines at field condition: Control, Control + 3 ºC, ABA and ABA + 3 ºC.

Effect of ultraviolet B radiation on pathogenic molds of grapes

The fungicidal effect of UV-C radiation (100-280 nm wavelength) is well known, but its applicability for the control of pathogenic molds of grapes is conditioned by its effect on the host and by the risks inherent in its handling[1].
As an alternative, the effect in vitro of UV-B radiation (280-315 nm) on the main pathogenic molds of grapes has been studied: Botrytis cinerea, Aspergillus niger, Penicillium expansum and Rhizopus stolonifer.

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.