terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Abstract

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities. Grapevine leaves being a disposable byproduct in viticulture, their potential valorization as a source of polyphenols is a topic of interest.

Our objective was to compare the diversity of grapevine leaves composition by screening qualitatively and quantitatively the polyphenol content in leaves of 50 cultivars grown in the same field of an experimental collection at Bordeaux INRAe. Fresh leaves were collected at the same period in summer, freeze-dried, ground to a fine powder and polyphenols were extracted twice with combination of organic solvents (100% and 70% methanol). A targeted HPLC-MS/MS approach was used for the quantification with available standards of 60 different polyphenols.

The results showed high variability in polyphenols content. Nevertheless, caftaric acid and quercetin 3-glucuronide were the major compounds detected in all leaves. Flavanols, and more particularly the ratio catechin/epicatechin, could be explored as markers to determine leaf cultivar, ranging from 0.5 to 42. Stilbenes were minor compounds in all leaves, found mainly in the forms of trans- and cis-piceid. Minor presence of resveratrol and its oligomers was assessed by HPLC-HRMS/MS.

Acknowledgements: The authors wish to thank the UE Viticole and Louis Bordenave for management of the experimental vineyard and the support of Bordeaux Metabolome

 

References:

(1) Lemaitre-Guillier et al (2021) VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine. https://doi.org/10.3390/molecules26144258

(2) Goufo et al (2020). A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves. doi: 10.3390/antiox9050398

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alan Jamain1, Margot Larose1, Andreu Mairata2, Manon Delapena1, Antonio Palos-Pinto1, Céline Franc1, Maria Lafargue3, Ghislaine Hilbert-Masson3, Stéphanie Cluzet1, Josep Valls Fonayet1

1Enology, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France
2Instituto de Ciencias de la Vid y el Vino, 26007 Logroño (La Rioja) ESPAÑA
3EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France

Contact the author*

Keywords

Vitis vinifera, Quercetin, Caftaric, Byproducts

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Phenolic composition and chromatic characteristics of blends of cv. Tempranillo wines from vines grown with different viticultural techniques in a semi-arid area

The quality and color stability of red wines are directly related to content and distribution of phenolic compounds. However, the climate change produces the asynchrony between the dates of technological and maturity of grapes. The crop-forcing technique (CF) restores the coupling between phenolic and technological ripeness while limits vineyard yields. Blending of wines is frequently used to equilibriate composition of wines and to increase their stability, color and quality. The aim of the present work is to study the phenolic composition and color of wine blends made with FW (wines from vines subjected to CF) and CW (wines for vines under the usual cultivation practices).

Prediction of aromatic attributes of red wines from its colour properties 

Wine perception is a multisensory experience that makes use of the sight, smell, and taste senses. When wine is sensorially assessed, the stimulus received generates multiple signals that tasters convert into organoleptic descriptors. Colour is commonly the first attribute evaluated during wine tasting. Moreover, the colour properties provide the taster with a priori information of the wine’s aroma. This preconceived perception is later confirmed or denied during the aroma evaluation.

Evaluation of terroir suitability for vine cultivation in new areas using geographic multi-criteria decision support

Based on historical vine cultivation, the recent development of wine production in Drama wine region (Greece) has led to vine cultivation expansion of white and red varieties. The current cultivation of 500 ha of vineyards is expected to increase in the coming years. Natural terroir units (NTU) have been designed recently to support the production of high quality wines in the region [1]. The aim of this work is to evaluate the relevancy of the proposed NTUs regarding their suitability to produce wines of specific sensorial identity, and to provide guidelines for correct site selection for the expanding wine industry of the region.

Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

In winemaking grapes, it is known that most aroma compounds are present as non-volatile precursors, such as glycosidic precursors. In fact, there is strong evidence supporting the connection between the content of aroma precursors and the aromatic quality of wine [1]. Acid hydrolysis is preferred to reveal the aroma potential of winemaking grapes, as it predicts more accurately the chemical rearrangements occurring during fermentation in acidic environments [2]. In this study, a method involving a fast fermentation followed by acid hydrolysis at 75ºC was used to evaluate the accumulation of aroma compounds over time in fractions obtained from six different varieties of winemaking grapes.

REGAVID a decision tool to deficit irrigation in a temperate climate (DO Monterrei – Spain)

In temperate climates, such as in the North of Spain, the use of irrigation in the vineyard has not been required, due to the usual rainfall from June to August. In some large vineyards, irrigation management has been carried out, based on occasional support irrigation, or for the application of nutrients (fertigation). Currently it is necessary to implement decision support models to manage irrigation water in real time and avoid misuse of a scarce resource. Moreover, quality standards must be achieved, as in the previous rainfed viticulture.