terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Abstract

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities. Grapevine leaves being a disposable byproduct in viticulture, their potential valorization as a source of polyphenols is a topic of interest.

Our objective was to compare the diversity of grapevine leaves composition by screening qualitatively and quantitatively the polyphenol content in leaves of 50 cultivars grown in the same field of an experimental collection at Bordeaux INRAe. Fresh leaves were collected at the same period in summer, freeze-dried, ground to a fine powder and polyphenols were extracted twice with combination of organic solvents (100% and 70% methanol). A targeted HPLC-MS/MS approach was used for the quantification with available standards of 60 different polyphenols.

The results showed high variability in polyphenols content. Nevertheless, caftaric acid and quercetin 3-glucuronide were the major compounds detected in all leaves. Flavanols, and more particularly the ratio catechin/epicatechin, could be explored as markers to determine leaf cultivar, ranging from 0.5 to 42. Stilbenes were minor compounds in all leaves, found mainly in the forms of trans- and cis-piceid. Minor presence of resveratrol and its oligomers was assessed by HPLC-HRMS/MS.

Acknowledgements: The authors wish to thank the UE Viticole and Louis Bordenave for management of the experimental vineyard and the support of Bordeaux Metabolome

 

References:

(1) Lemaitre-Guillier et al (2021) VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine. https://doi.org/10.3390/molecules26144258

(2) Goufo et al (2020). A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves. doi: 10.3390/antiox9050398

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alan Jamain1, Margot Larose1, Andreu Mairata2, Manon Delapena1, Antonio Palos-Pinto1, Céline Franc1, Maria Lafargue3, Ghislaine Hilbert-Masson3, Stéphanie Cluzet1, Josep Valls Fonayet1

1Enology, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France
2Instituto de Ciencias de la Vid y el Vino, 26007 Logroño (La Rioja) ESPAÑA
3EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France

Contact the author*

Keywords

Vitis vinifera, Quercetin, Caftaric, Byproducts

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Plastic debris at vines: carriers of pollutants in the environment?

Modern agriculture employs large amounts of plastics, such as mulching and greenhouse films, thermal covers, plant protection tubes and tying tape. The latter two types are very common in viticulture. Guard tubes are employed to protect young vines from mechanic and atmospheric damage, whilst polymeric tying tape has replaced natural-origin materials to hold the canopy of vines. Both materials are made on synthetic polymers, which include a range of additives to improve their environmental stability remaining in the environment of vineyards for years. During this time, they are exposed to the range of pesticides (fungicides, insecticides and in a lesser extend herbicides) applied to vines.

Agronomic and oenological behavior of the minority Mandón variety on two rootstocks in the D.O. Arribes

A large population of vines of the Mandón minority red variety (synonymous with Garró) has been located in old vineyards of the D.O. Arribes (Zamora and Salamanca) to conserve and recover this minority variety. The wines made with this variety are characterized by their good structure and color, interesting harmony, an excellently low pH, with high acidity, as well as complex aromas of blue fruits and a marked and expressive minerality.

Applicability of spectrofluorometry and voltammetry in combination with machine learning approaches for authentication of DOCa Rioja Tempranillo wines

The main objective of the work was to develop a simple, robust and selective analytical tool that allows predicting the authenticity of Tempranillo wines from DOCa Rioja. The techniques of voltammetry and absorbance-transmission and fluorescence excitation emission matrix (A-TEEM) spectroscopy have been applied in combination with machine learning (ML) algorithms to classify red wines from DOCa Rioja according to region (Alavesa, Alta or Oriental) and category (young, crianza or reserva).

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison. In all solutions were added 1ml/l of Tween 80 ® surfactant.

New food trend ahead? Highlighting the nutritional benefits of grapevine leaves

The wine industry produces an enormous amount of waste every year. A wider inclusion of disregarded by-products in the human diet or its use as a source of bioactive compounds is a good strategy for reducing waste. It will not only introduce an added value to a waste product but also come upon the European Union and United Nations’ demands towards more sustainable agricultural approaches and circular economy.