terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Abstract

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities. Grapevine leaves being a disposable byproduct in viticulture, their potential valorization as a source of polyphenols is a topic of interest.

Our objective was to compare the diversity of grapevine leaves composition by screening qualitatively and quantitatively the polyphenol content in leaves of 50 cultivars grown in the same field of an experimental collection at Bordeaux INRAe. Fresh leaves were collected at the same period in summer, freeze-dried, ground to a fine powder and polyphenols were extracted twice with combination of organic solvents (100% and 70% methanol). A targeted HPLC-MS/MS approach was used for the quantification with available standards of 60 different polyphenols.

The results showed high variability in polyphenols content. Nevertheless, caftaric acid and quercetin 3-glucuronide were the major compounds detected in all leaves. Flavanols, and more particularly the ratio catechin/epicatechin, could be explored as markers to determine leaf cultivar, ranging from 0.5 to 42. Stilbenes were minor compounds in all leaves, found mainly in the forms of trans- and cis-piceid. Minor presence of resveratrol and its oligomers was assessed by HPLC-HRMS/MS.

Acknowledgements: The authors wish to thank the UE Viticole and Louis Bordenave for management of the experimental vineyard and the support of Bordeaux Metabolome

 

References:

(1) Lemaitre-Guillier et al (2021) VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine. https://doi.org/10.3390/molecules26144258

(2) Goufo et al (2020). A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves. doi: 10.3390/antiox9050398

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alan Jamain1, Margot Larose1, Andreu Mairata2, Manon Delapena1, Antonio Palos-Pinto1, Céline Franc1, Maria Lafargue3, Ghislaine Hilbert-Masson3, Stéphanie Cluzet1, Josep Valls Fonayet1

1Enology, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France
2Instituto de Ciencias de la Vid y el Vino, 26007 Logroño (La Rioja) ESPAÑA
3EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France

Contact the author*

Keywords

Vitis vinifera, Quercetin, Caftaric, Byproducts

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Irrigation frequency in four grapevine red varieties in Spain. Effect on must volatile composition

The irrigation water management in the vineyard is a crucial aspect to obtain sustainable quality production over time. Previous studies have set the water requirements to be applied in the vineyard at 30 % of the reference evapotranspiration (ET0), although there are no studies that settle the effects of the frequency of irrigation application on red varieties in Spain. The present study contemplates the application of deficit irrigation (30 % ET0) applying a weekly dose in a single irrigation (T07) or in two irrigation events (T03) per week. The study has been carried out in 2021-2022 with four red varieties in different Spanish wine regions: Garnacha Tinta (Badajoz), Tempranillo (Valladolid), Syrah (Albacete) and Mencía (Lugo). The effects of irrigation frequency on must volatile composition have been evaluated through GC-MS.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Rootstocks not only provide tolerance to Phylloxera, but also ensure the supply of water and mineral nutrients to the whole plant. Rootstocks are an important way of adapting to environmental conditions while conserving the typical features of scion varieties. We can exploit the large diversity of rootstocks used worldwide to aid this adaptation. The aim of this study was to characterise rootstock regulation of scion mineral status and its relation with scion development.

Wine racking in the winery and the use of inerting gases

The O2 uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O2 uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The objective was to study O2 uptake during the racking of a model wine without using inert gases and to compare it with the purging of the destination tank with different inert gases.

Characterization of a Sémillon clonal population: exploring genetic diversity, metabolomic profiles, and phenotypic variations

Sémillon is a cultivated grape variety known for contributing to dry and sweet white wine production. However, only seven approved clones have been officially recognized in France[1]. In this study, we aimed to characterize the genetic diversity and metabolomic profiles of a Sémillon clonal population, shedding light on the potential variations within this important grape variety.

Biotype diversity within the autochthonous ‘Bobal’ grapevine variety

Bobal is the second most widely grown Spanish red grape variety (54,165 has), mainly cultivated in the Valencian Community and especially, in Utiel-Requena region (about 67% of 34,000 has). In this study, agronomic and enological parameters were determined in 98 biotypes selected during 2018 and 2019 in more than 50 vineyards over 50 years-old in the Utiel-Requena region. Moreover, a multi-criteria approach considering temperature and rainfall (Fig. 1A), among other parameters, was made to establish three different zones within the region (Fig. 1B), where in the future the selected biotypes will evaluated. In fact, in 2020, 4 replicates and 12 vines per biotype were planted in an experimental vineyard to preserve this important intra-cultivar diversity.