terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Abstract

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain. To address this gap, a trial was conducted to assess whether MeJ and BTH application could enhance the biosynthesis of PC involved in the defense mechanisms of Monastrell seedlings. The trial involved grapevine seedlings of the Monastrell variety grown in individual pots in a controlled environment. Four treatments were administered, including water (control), MeJ, BTH, and a combination of MeJ and BTH. Leaf samples were collected at various time intervals, and the quantification of stilbenes and flavonols was carried out. The results demonstrated that the elicitor treatments positively influenced the biosynthesis of stilbenes and flavonols. The application of MeJ led to significant increases in the production of key grapevine antimicrobial stilbenes, as well as some flavonols, particularly at 18-hours after treatment. These increases remained above control levels throughout the trial. The effects of BTH and MeJ+BTH treatments were less pronounced compared to MeJ alone, with the highest increase observed at 24-hours after treatment. However, they were always greater than the control. Overall, the findings suggest that the application of MeJ and BTH has the potential to improve the defence mechanisms of Monastrell vines, reducing reliance on chemical treatments. Further research is needed to validate the elicitor activity of MeJ and BTH against common grapevine diseases.

DOI:

Publication date: October 9, 2023

Issue: ICGWS 2023

Type: Poster

Authors

D. Paladines-Quezada1*, J. D. Moreno-Olivares2, M. J. Giménez-Bañón2, J. A. Bleda-Sánchez, A. Cebrián-Pérez, J. C. Gómez-Martínez, J. I. Fernández-Fernández2 y Rocío Gil-Muñoz2

1Grupo VIENAP, Instituto de Ciencias de la Vid y del Vino (CSIC, Universidad de La Rioja, Gobierno de La Rioja), Ctra. de Burgos, km. 6, 26007 (Logroño, Spain).
2Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA). Ctra. La Alberca s/n, 30150 (Murcia, Spain).

Contact the author*

Keywords

stilbenes, induced resistance, elicitor, vineyard

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Antimicrobial activity of oenological polyphenols against Gram positive and Gram negative intestinal multidrug-resistant bacteria

Bacterial antibiotic resistance is a major current health problem. Polyphenols have demonstrated antibacterial activity, and in this work we studied the effect of oenological polyphenols on the growth of intestinal multidrug-resistant strains of human and animal origin. Two Enterococcus faecium strains, resistant to vancomycin and other antibiotics, and four Escherichia coli strains, resistant to ampicillin and other antibiotics, were included in this study. All strains showed multidrug resistant phenotypes and genotypes to at least two antibiotic families.

Genetic identification of 200-year-old Serbian grapevine herbarium

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions.

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.
We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin.

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.

Correlative study between degradation of rosé wine under accelerated conditions and under normal conditions

Several studies have tried to develop different methods to study the photodegradation of wine in an accelerated way, trying to elucidate the effect of light on the wine compounds[1]. In a previous study, our team developed a chamber that speeds up the photodegradation of rosé wine[2]. In the present work we have tried to establish a correlation between irradiation times in accelerated conditions and the natural exposure to the cycles of light that usually exist in markets or at home.