terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Abstract

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock. Considering the increasing pressure of the biotic and abiotic stresses, it is utmost necessary to also evaluate the effects of drought on the microbiome associated to grapevine in a sensitive Mediterranean region (Alentejo – Portugal).

In this study we characterize the diversity and the structure of the soil microbial community of the drought tolerant Syrah cultivar under long-term irrigation experiment (five years) with three levels (100% ETc – FI; 50% ETc – DI; rain-fed – NI). Metabarcoding of bacteria (16S rRNA subregion) and fungi (ITS region) was applied on the same soil samples. Also soil chemical analysis are being integrated with genomic data.

Although the richness and evenness indexes for alpha diversity did not show strong differences among the irrigation strategies for neither of the targeted microorganisms, beta diversity revealed statistically supported community differentiation. Across all samples the top three bacterial phyla were Pseudomonadota, Actinobacteriota, and Bacteroidota with a total relative abundance of 60%. Regarding the most represented bacterial species across samples, Gaiella occulta, an uncultured actinobacteria first described in deep mineral waters in Portugal, is shown with prevalence in DI samples with more than 10% of total ASVs.

Next, we will predict communities functionalities, bacterial networks, according to soil chemistry data and compare them with the soils’ samples obtained in July 2023.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Gianmaria Califano1,2*, Júlio Maciel1Olfa Zarrouk3,4, Miguel Damásio5, Jose Silvestre5, Ana Margarida Fortes1,2

1Faculdade de Ciências, University of Lisbon, Portugal
2BioISI, Faculdade de Ciências, University of Lisbon, Portugal
3LEAF – Linking Landscape, Environment, Agriculture and Food Research Centre, Associate Laboratory TERRA, ISA-ULisboa, Lisboa, Portugal
4COLAB, Torres Vedras, Portugal
5INIAV, Polo de Dois Portos, Portugal

Contact the author*

Keywords

soil microbiome, metabarcoding, grapevine, Syrah, drought, crop sustainability

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Chemical profiling and sensory analysis of wines from resistant hybrid grape cultivars vs conventional wines

Recently, there has been a shift toward sustainable wine production, according to EU policy (F2F and Green Deal), to reduce pesticide usage, improve workplace health and safety, and prevent the impacts of climate change. These trends have gained the interest of consumers and winemakers. The cultivation of disease resistant hybrid grape cultivars (DRHGC), known as ‘PIWI’ grapes can help with these objectives [1]. This study aimed to profile white and red wines produced from DRHGC in South Tyrol (Italy). Wines produced from DRHGCs were compared with conventional wines produced by the same wineries. The measured parameters were residual sugars, organic acids, alcohol content, pigments and other phenolics by LC-QqQ/MS, colorimetric indexes (CIELab); and volatile profiles (HS-SPME-GCxGC-ToF/MS [2]).

The potential of some native varieties of Argentina for the production of sparkling wines. Effect of lees contact time 

Grapevine varieties from South-America, commonly known as criollas, originated because of the natural crossbreeding of grapevine varieties brought by the Spaniards. The objective of this work was to evaluate the potential of some varieties to produce sparkling wines considering the effect of lees contact time. The following varieties were used: Moscatel Rosado, Criolla Chica, Pedro Gimenez, Blanca Oval, Canelón, and the European variety Chardonnay (control), planted in the ampelographic collection of EEA Mendoza INTA (Argentina). Pilot-scale vinifications were carried out to obtain the base wines, in 20 L glass containers. The second fermentation was performed through the traditional method.

Phenolic composition of Cabernet Sauvignon wines from Argentina, Portugal and Spain

Grape and wine phenolic compounds have been shown to be highly related to both wine quality (color, flavor, and taste) and health-promoting properties (antioxidant and cardioprotective, among others). The aim of this work was to evaluate and compare the phenolic contents of Cabernet Sauvignon wines from different geographical areas and climatic conditions, namely from Argentina, Portugal and Spain vintage 2022. In addition, the phenolic profiles of the Portuguese wines from three vintages (2020, 2021, 2022) was compared.

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.

Symbiotic microorganisms application in vineyards: impacts on grapevine performance and microbiome

Microorganism-based inoculants have been suggested as a viable solution to mitigate the adverse effects of climate change on viticulture. However, the actual effectiveness of these inoculants when applied under field conditions remains a challenge, and their effects on the existing soil microbiota are still uncertain. This study investigates the impact of arbuscular mycorrhizal fungi inoculation on grapevine performance and microbiome. The study was conducted in a vineyard of Callet cultivar in Binissalem, Mallorca, Spain. Two different treatments were applied: control and inoculation with commercial mycorrhizae complex of Rhizoglomus irregulare applied to plants through irrigation.