terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Abstract

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock. Considering the increasing pressure of the biotic and abiotic stresses, it is utmost necessary to also evaluate the effects of drought on the microbiome associated to grapevine in a sensitive Mediterranean region (Alentejo – Portugal).

In this study we characterize the diversity and the structure of the soil microbial community of the drought tolerant Syrah cultivar under long-term irrigation experiment (five years) with three levels (100% ETc – FI; 50% ETc – DI; rain-fed – NI). Metabarcoding of bacteria (16S rRNA subregion) and fungi (ITS region) was applied on the same soil samples. Also soil chemical analysis are being integrated with genomic data.

Although the richness and evenness indexes for alpha diversity did not show strong differences among the irrigation strategies for neither of the targeted microorganisms, beta diversity revealed statistically supported community differentiation. Across all samples the top three bacterial phyla were Pseudomonadota, Actinobacteriota, and Bacteroidota with a total relative abundance of 60%. Regarding the most represented bacterial species across samples, Gaiella occulta, an uncultured actinobacteria first described in deep mineral waters in Portugal, is shown with prevalence in DI samples with more than 10% of total ASVs.

Next, we will predict communities functionalities, bacterial networks, according to soil chemistry data and compare them with the soils’ samples obtained in July 2023.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Gianmaria Califano1,2*, Júlio Maciel1Olfa Zarrouk3,4, Miguel Damásio5, Jose Silvestre5, Ana Margarida Fortes1,2

1Faculdade de Ciências, University of Lisbon, Portugal
2BioISI, Faculdade de Ciências, University of Lisbon, Portugal
3LEAF – Linking Landscape, Environment, Agriculture and Food Research Centre, Associate Laboratory TERRA, ISA-ULisboa, Lisboa, Portugal
4COLAB, Torres Vedras, Portugal
5INIAV, Polo de Dois Portos, Portugal

Contact the author*

Keywords

soil microbiome, metabarcoding, grapevine, Syrah, drought, crop sustainability

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

In winemaking grapes, it is known that most aroma compounds are present as non-volatile precursors, such as glycosidic precursors. In fact, there is strong evidence supporting the connection between the content of aroma precursors and the aromatic quality of wine [1]. Acid hydrolysis is preferred to reveal the aroma potential of winemaking grapes, as it predicts more accurately the chemical rearrangements occurring during fermentation in acidic environments [2]. In this study, a method involving a fast fermentation followed by acid hydrolysis at 75ºC was used to evaluate the accumulation of aroma compounds over time in fractions obtained from six different varieties of winemaking grapes.

Effect of riboflavin on the longevity of white and rosé wines

Light is a fundamental part at sales points which influences in the conservation of wines, particularly in those that are sold in transparent glass bottles such as rosé wines and increasingly white wines. The photochemical effect known as “light-struck taste” can cause changes in the aromatic characteristics of the wine. This “light-struck taste” is due to reactions triggered by the photochemical sensitivity of riboflavin (RBF).

Genetic identification of 200-year-old Serbian grapevine herbarium

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions.

Extreme vintages affect grape varieties differently: a case study from a cool climate wine region

Eger wine region is located on the northern border of grapevine cultivation zone. In the cool climate, terroir selection is one of the foundations of quality wine making. However, climate change will have a significant impact on these high value-added vineyards. This study presents a case study from 2021 and 2022 with the investigation of three grape varieties (Kadarka, Syrah, Furmint). The experiment was conducted in a steep-sloped vineyard (Nagy-Eged hill) with a southern exposure.

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution.