terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Abstract

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock. Considering the increasing pressure of the biotic and abiotic stresses, it is utmost necessary to also evaluate the effects of drought on the microbiome associated to grapevine in a sensitive Mediterranean region (Alentejo – Portugal).

In this study we characterize the diversity and the structure of the soil microbial community of the drought tolerant Syrah cultivar under long-term irrigation experiment (five years) with three levels (100% ETc – FI; 50% ETc – DI; rain-fed – NI). Metabarcoding of bacteria (16S rRNA subregion) and fungi (ITS region) was applied on the same soil samples. Also soil chemical analysis are being integrated with genomic data.

Although the richness and evenness indexes for alpha diversity did not show strong differences among the irrigation strategies for neither of the targeted microorganisms, beta diversity revealed statistically supported community differentiation. Across all samples the top three bacterial phyla were Pseudomonadota, Actinobacteriota, and Bacteroidota with a total relative abundance of 60%. Regarding the most represented bacterial species across samples, Gaiella occulta, an uncultured actinobacteria first described in deep mineral waters in Portugal, is shown with prevalence in DI samples with more than 10% of total ASVs.

Next, we will predict communities functionalities, bacterial networks, according to soil chemistry data and compare them with the soils’ samples obtained in July 2023.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Gianmaria Califano1,2*, Júlio Maciel1Olfa Zarrouk3,4, Miguel Damásio5, Jose Silvestre5, Ana Margarida Fortes1,2

1Faculdade de Ciências, University of Lisbon, Portugal
2BioISI, Faculdade de Ciências, University of Lisbon, Portugal
3LEAF – Linking Landscape, Environment, Agriculture and Food Research Centre, Associate Laboratory TERRA, ISA-ULisboa, Lisboa, Portugal
4COLAB, Torres Vedras, Portugal
5INIAV, Polo de Dois Portos, Portugal

Contact the author*

Keywords

soil microbiome, metabarcoding, grapevine, Syrah, drought, crop sustainability

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of different plant fibers on the elimination of undesirable compounds in red wine. Correlation with its polysaccharide composition

The presence of undesirable compounds in wines, such as OTA, biogenic amines and pesticides residues, affects wine quality and can cause health problems for the consumer. The main tool that a winemaker has to reduce their content in the wine is fining. However, some of the fining agents commonly used in the winery can cause allergies or even increase the protein content in the wine, increasing the turbidity. To avoid these problems, the use of plant fibers may be an alternative, such as those from grape pomace[1] or other plant origins.

Exploring relationships among grapevine chemical and physiological parameters and mycobiome composition under drought stress

Improving our knowledge on biotic and abiotic factors that influence the composition of the grapevine mycobiome is of great agricultural significance, due to potential effects on plant health, productivity, and wine characteristics. Among the various environmental factors affecting the morphological, physiological, biochemical and molecular attributes of grapevine, drought stress is one of the most severe, becoming increasingly an issue worldwide.

The weak role of organic mulches in shaping bacterial communities in grapevine

The interest in sustainable and ecologic agricultural practices in grapevine has grown significantly in recent years in the context of ecological transition. Organic mulches are treatments that support the circular economy and positively affect the soil and the plant. They are an alternative to herbicides and other conventional practices since they may influence soil moisture, erosion, structure and weed control. However, their effects on the soil and must microbiota remain unknown.

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.