terclim by ICS banner
IVES 9 IVES Conference Series 9 CHANGES IN METABOLIC FLUXES UNDER LOW PH GROWTH CONDITIONS: CAN THE SLOWDOWN OF CITRATE CONSUMPTION IMPROVE OENOCOCCUS OENI ACID-TOLERANCE?

CHANGES IN METABOLIC FLUXES UNDER LOW PH GROWTH CONDITIONS: CAN THE SLOWDOWN OF CITRATE CONSUMPTION IMPROVE OENOCOCCUS OENI ACID-TOLERANCE?

Abstract

Oenococcus oeni is the main Lactic Acid Bacteria responsible for malolactic fermentation, converting malic acid into lactic acid and carbon dioxide in wines. Following the alcoholic fermentation, this second fermentation ensures a deacidification and remains essential for the release of aromatic notes and the improvement of microbial stability in many wines. Nevertheless, wine is a harsh environment for microbial growth, especially because of its low pH (between 2.9 and 3.6 depending on the type of wine) and nutrient deficiency. In order to maintain homeostasis and ensure viability, O. oeni possesses different cellular mechanisms including organic acid metabolisms which represent also the major pathway to synthetize energy in wine. Indeed, uptake and consumption of malate and citrate by this bacteria enables to activate a proton motive force (PMF) hence maintaining an intracellular pH by proton consumption1,2.

Citrate is found in wine at small concentrations (0.13 to 0.90 g/L). It can be metabolized by O. oeni into acetate, pyruvate and then aromatic compounds such as diacetyl, acetoin and 2,3-butanediol. The ability of citrate metabolism to activate the PMF could play a central role in the acid-tolerance of this bacterium. Nevertheless, a previous study has described an inhibition of O. oeni growth at low pH in presence of high amounts of citrate3. This toxic effect could come from the synthesis of one of the citrate metabolites as acetate.

In order to understand how citrate metabolism can be linked to acid tolerance of this bacterium, consumption of citrate was investigated in a great diversity of O. oeni strains. In addition, malate and sugar consumptions were also followed, as they can be impacted by citrate metabolism. These experiments enabled to draw metabolic fluxes in O. oeni according to the pH of the medium. In most cases, ma- late is first metabolized, then citrate and sugars, sequentially, proving that the priority is given to organic acid consumption at the expense of sugars in this bacterium. However, this experiment revealed different citrate consumption profiles which may be correlated to a greater or lesser acid tolerance according to the strain. Furthermore, a genomic comparison demonstrated the presence of mutations in the citrate operon of acid-tolerant industrial strains. Hence, acid tolerance could be linked to a change in the rate of citrate consumption in O. oeni.

 

1. Ramos, A., Poolman, B., Santos, H., Lolkema, J.S., Konings, W.N., 1994. Uniport of anionic citrate and proton consumption in ci-trate metabolism generates a proton motive force in Leuconostoc oenos. J. Bacteriol. 176, 4899–4905. https://doi.org/10.1128/jb.176.16.4899-4905.1994
2. Salema, M., Lolkema, J.S., Romão, M.V.S., Dias, M.C.L., 1996. The proton motive force generated in Leuconostoc oenos by L-malate fermentation. J. Bacteriol. 178, 3127–3132. https://doi.org/10.1128/jb.178.11.3127-3132.1996
3. Augagneur, Y., Ritt, J.-F., Linares, D.M., Remize, F., Tourdot-Maréchal, R., Garmyn, D., Guzzo, J., 2007. Dual effect of organic acids as a function of external pH in Oenococcus oeni. Arch. Microbiol. 188, 147–157. https://doi.org/10.1007/s00203-007-0230-0

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Camille Eicher1, Joana Coulon2, Marion Favier2, Edouard Munier1, Thierry Tran1, Hervé Alexandre1, Cristina Reguant Miran-da3, Cosette Grandvalet1

1. UMR A. 02.102 Procédés Alimentaires et Microbiologiques, L’institut Agro Dijon, Université de Bourgogne Franche-Comté, Dijon, France
2. BioLaffort, Floirac, France
3. Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Universitat Rovira i Virgili, Tarragona, Spain

Contact the author*

Keywords

Oenococcus oeni, Citrate, Metabolic fluxes, Acid-tolerance

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

ASSESSMENT OF ‘DOLCETTO’ GRAPES AND WINES FROM DIFFERENT AREAS OF OVADA DOCG

Dolcetto (Vitis vinifera L.) is one of the traditionally cultivated varieties in Piedmont (north-east Italy). Dolcetto wines have long been associated with local consumption and they are little known internationally. In particular, the Ovada area (south-east Piedmont), even if it represents a small share of the regional PDO Dolcetto production, is one of the oldest and vocated territory, giving wine also suitable for aging. In this study, the basic composition and phenolic content of Dolcetto grapes for Ovada DOCG wines have been investigated in three different vintages (2020-2022), as well as the main aspects of the derived commercial and experimental wines (basic parameters, phenolics, volatile compounds, sensory properties).

IDENTIFYING POTENTIAL CHEMICAL MARKERS RESPONSIBLE FOR THE PERMISSIVENESS OF BORDEAUX RED WINES AGAINST BRETTANOMYCES BRUXELLENSIS USING UNTARGETED METABOLOMICS

All along the red winemaking process, many microorganisms develop in wine, some being beneficial and essential, others being feared spoilers. One of the most feared microbial enemy of wine all around the world is Brettanomyces bruxellensis. Indeed, in red wines, this yeast produces volatile phenols, molecules associated with a flavor described as “horse sweat”, “burnt plastic” or “leather”. To produce significant and detectable concentrations of these undesired molecules, the yeasts should first grow and become numerous enough. Even if the genetic group of the strain present and the cellar temperature may modulate the yeast growth rate¹ and thus the risk of spoilage, the main factor seems to be the wines themselves, some being much more permissive to B. bruxellensis development than others.

AROMA AND SENSORY CHARACTERIZATION OF XINOMAVRO RED WINES FROM DIFFERENT GREEK PROTECTED DESIGNATIONS OF ORIGIN, EFFECT OF TERROIR CHARACTERISTICS

The quality of wines has often been associated with their geographical area of production. The aim of this work was to characterize Protected Designation of Origin (PDO) Xinomavro red wines from different geographical areas of Amyndeon and Naoussa in Northern Greece, elaborated with variables that contribute to their differentiation, such as soil characteristics, altitude, monthly average temperature and rainfall.
Xinomavro fruit parcels from different vineyards within the two PDO zones (5 PDO Naoussa and 6 PDO Amyndeon) were vinified following a standard winemaking process. A total of 25 aroma compounds were quantified using gas chromatography-mass spectrometry (GC-MS) with simultaneous full scan and selected ion monitoring for data recording, and odor activity values (OAVs) were determined.

‘TROPICAL’ POLYFUNCTIONAL THIOLS AND THEIR ROLE IN AUSTRALIAN RED WINES

Following anecdotal evidence of unwanted ‘tropical’ character in red wines resulting from vineyard interventions and a subsequent yeast trial observing higher ‘red fruit’ character correlated with higher thiol concentrations, the role of polyfunctional thiols in commercial Australian red wines was investigated.
First, trials into the known tropical thiol modulation technique of foliar applications of sulfur and urea were conducted in parallel on Chardonnay and Shiraz.1 The Chardonnay wines showed expected results with elevated concentrations of 3-sulfanylhexanol (3-SH) and 3-sulfanylhexyl acetate (3-SHA), whereas the Shiraz wines lacked 3-SHA. Furthermore, the Shiraz wines were described as ‘drain’ (known as ‘reductive’ aroma character) during sensory evaluation although they did not contain thiols traditionally associated with ‘reductive’ thiols (H2S, methanethiol etc.).

PINKING PHENOMENA ON WHITE WINES: RELATION BETWEEN PINKING SUSCEPTIBILITY INDEX (PSI) AND WINE ANTHOCYANINS CONTENT

Pinking is the emergence of pink tones in white wines exclusively produced from white grape varieties, known as pinking phenomena for many years. Pinking is essentially appeared when white wines are produced under reducing conditions [1,2,3]. Pinking usually occurs after bottling and storage of white wines, but its appearance has also been described after alcoholic fermentation or even as soon as the grape must is extracted [4]. Therefore, the purpose of this work was to investigate the existence of an-thocyanins in white wines made from different white grape varieties and grown locations and critically evaluate the most common method used for predicting pinking appearance in white wines: the Pinking Susceptibility Index (PSI).