terclim by ICS banner
IVES 9 IVES Conference Series 9 INCREASING PINOT NOIR COLOUR DENSITY THROUGH SEQUENTIAL INOCULATION OF FLOCCULENT COMMERCIAL WINE YEAST SPECIES

INCREASING PINOT NOIR COLOUR DENSITY THROUGH SEQUENTIAL INOCULATION OF FLOCCULENT COMMERCIAL WINE YEAST SPECIES

Abstract

Vitis vinifera L. cv. Pinot noir can be challenging to manage in the winery as its thin skins require careful handling to ensure sufficient extraction of wine colour to promote colour stability during ageing.1 Literature has shown that fermentation with flocculent yeasts can increase red wine colour density.2 As consumers prefer greater colour density in red wines,3 the development of tools to increase colour density would be useful for the wine industry. This research explored the impact of interspecies sequential inoculation and co-flocculation of commercial yeast on Pinot noir wine colour. Six commercial non-Saccharomyces yeast species and two commercial Saccharomyces cerevisiae strains were assayed based on their sedimentation rates in synthetic grape must, both individually and in combination, to determine flocculation ability. The most flocculent S. cerevisiae and non-Saccharomyces spp. yeast pairings, RC212 + BIODIVA and VL3 + BIODIVA, were used in a 20 L-scale Pinot noir winemaking trial. Ul- traviolet-visible spectrophotometric measurements of wine colour parameters, and sensory evaluation of wine appearance, found that mixed species fermentations produced wines with greater colour density. Total and monomeric anthocyanin concentrations were lower in sequentially-inoculated wines, despite being the main source of young red wine colour. Pigmentation assays indicated a higher adsorption of anthocyanins by BIODIVA than S. cerevisiae, suggesting that greater amounts of cell wall mannoproteins in flocculent yeast may scavenge anthocyanins during fermentation, allowing for their subsequent release from the lees and potential for enhanced formation of copigments. Findings from this research have wide application in the industry to increase red wine colour intensity, particular in thin- skinned red grape varieties.

 

1. Carew, A. L.; Smith, P.; Close, D. C.; Curtin, C.; Dambergs, R. G. Yeast Effects on Pinot Noir Wine Phenolics, Color, and Tannin Composition. J. Agric. Food Chem. 2013, 61 (41), 9892–9898. https://doi.org/10.1021/jf4018806.
2. Varela, C.; Bartel, C.; Nandorfy, D. E.; Borneman, A.; Schmidt, S.; Curtin, C. Identification of Flocculant Wine Yeast Strains with Improved Filtration-Related Phenotypes through Application of High-Throughput Sedimentation Rate Assays. Sci. Rep. 2020, 10 (1). https://doi.org/10.1038/s41598-020-59579-y.
3. Parpinello, G. P.; Versari, A.; Chinnici, F.; Galassi, S. Relationship among Sensory Descriptors, Consumer Preference and Color Parameters of Italian Novello Red Wines. Food Res. Int. 2009, 42 (10), 1389–1395. https://doi.org/10.1016/j.foodres.2009.07.005.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Katasha S. MCCULLOUGH1,2, Yi YANG2, Melodie A. LINDSAY2 and Neill CULLEY2

1. School of Biological Sciences, The University of Auckland
2. School of Chemical Sciences, The University of Auckland

Contact the author*

Keywords

Anthocyanins, Colour density, Pinot noir, Sequential inoculation

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

PROGRESS OF STUDIES OF LEES ORIGINATING FROM THE FIRST ALCOHOLIC FERMENTATION OF CHAMPAGNE WINES

Champagne wines are produced via a two-step process: the first is an initial alcoholic fermentation of grape must that produces a still base wine, followed by a second fermentation in bottle – the prise de mousse – that produces the effervescence. This appellation produces non-vintage sparkling wines composed of still base wines assembled from different vintages, varieties, and regions. These base wines, or “reserve wines,” are typically conserved on their fine lies and used to compensate for quality variance between vintages (1). Continuously blending small amounts of these reserve wines into newer ones also facilitates preserving the producer’s “house style.”

EVIDENCE OF THE INTERACTION OF ULTRASOUND AND ASPERGILLOPEPSINS I ON UNSTABLE GRAPE PROTEINS

Most of the effects of ultrasound (US) result from the collapse of bubbles due to cavitation. The shockwave produced is associated with shear forces, along with high localised temperatures and pressures. However, the high-speed stream, radical species formation, and heat generated during sonication may also affect the stability of some enzymes and proteins, depending on their chemical structure. Recently, Ce-lotti et al. (2021) reported the effects of US on protein stability in wines. To investigate this further, the effect of temperature (40°C and 70°C; 60s), sonication (20 kHz and 100 % amplitude, for 20s and 60s, leading to the same temperatures as above, respectively), in combination with Aspergillopepsins I (AP-I) supplementation (100 μg/L), was studied on unstable protein concentration (TLPs and chitinases) using HPLC with an UV–Vis detector in a TLPs-supplemented model system and in an unstable white wine.

EFFECT OF WHOLE BUNCH VINIFICATION ON THE ABUNDANCE OF A SWEETENING COMPOUND

In classic red wine-making process, grapes are usually destemmed between harvest and the filling of the vat. However, some winemakers choose to let all or a part of the stems in contact with the juice during vatting, this is called whole bunch vinification. For instance, this practice is traditionally used in some French wine regions, notably in Burgundy, Beaujolais and the Rhone Valley. The choice to keep this part of the grape is likely to affect the sensory properties of wine, as its gustatory perception1,2.

EFFECT OF DIFFERENT VITICULTURAL AND ENOLOGICAL PRACTICES ON THE PHENOLIC COMPOSITION OF RED WINES

Global climate change is exerting a notable influence on viticulture sector and grape composition. The increase in temperature and the changes in rainfall pattern are causing a gap between phenolic and technological grape maturities [1]. As a result, the composition of grapes at harvest time and, consequently, that of wines are being affected, especially with regards to phenolic composition. Hence, wine quality is decreasing due to changes in the organoleptic properties, such as color and astringency, making necessary to implement new adaptive technologies in wineries to modulate these properties in order to improve wine quality.

ANTI-TRANSPIRANT MODULATION OF GRAPE RIPENING: EFFECTS ON MERLOT VINE DEVELOPMENT AND ROSÉ WINE PHENOLIC AND AROMATIC PROFILES

Climate changes are impacting viticultural regions throughout the world with temperature increases being most prevalent.1 These changes will not only impact the regions capable of growing grapes, but also
the grapes that can be grown.2 As temperatures rise the growing degree days increase and with it the sugar accumulation within the berries and subsequent alcohol levels in wine. Consequently, viticultural
practices need to be examined to decrease the levels of sugars.