terclim by ICS banner
IVES 9 IVES Conference Series 9 THE IMPACT OF NON-SACCHAROMYCES YEASTS ON THE WHITE WINE QUALITY

THE IMPACT OF NON-SACCHAROMYCES YEASTS ON THE WHITE WINE QUALITY

Abstract

Selected strains of non-Saccharomyces yeasts showed a positive effect on sensory characteristics and aromatic complexity of wine. A sequential microbial culture of non-Saccharomyces and S. cerevisiae species is usually inoculated due to poorer fermentability of non-Saccharomyces species. The aim of the study was to investigate the role of non-Saccharomyces yeasts in the production of white wines. We evaluated how individual combinations of sequential inoculations of non-Saccharomyces and S. cerevisiae species affect the aromatic compounds (volatile thiols and esters) and sensory characteristics of the wines. Sauvignon Blanc and Istrian Malvasia musts were inoculated sequentially with different species of non-Saccharomyces yeasts (Pichia kluyveri FrootZen (Chr. Hansen Holding A/S), Kluyveromyces dobzhanskii Re19L, Pichia guilliermondii ZIM624, Starmerella orientalis 126, Torulaspora delbrueckii IVV7, Lachancea thermotolerans BLF LT7 (Laffort),) and yeasts of the Saccharomyces genus (S. cerevisiae Zymaflore X5 (Laffort), S. uvarum NO608/1, S. cerevisiae Ca39). Fermentation kinetics and reducing sugars content were monitored gravimetrically during alcoholic fermentation. After completion of alcoholic fermentation, physicochemical analyses were performed, and the content of volatile thiols and esters was determined by GC-MS and the content of hydroxycinnamates by HPLC-DAD. We also performed sensory analysis using intensity ranking test. The mixed yeast cultures showed differences in fermentation kinetics, in the ability to release thiols, and in the synthesis of esters during alcoholic fermentation. According to the sensory evaluation, the best evaluated wine was produced with the yeast K. dobzhanskii Re19L in the vinification of Sauvignon Blanc and with T. delbrueckii IVV7 in the Istrian Malvasia variety.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Lorena Butinar1, Patricija Ploj-Jesenko2, Guillaume Antalick1, Melita Sternad Lemut1, Mitja Martelanc1, Katja Šuklje3, Andreja Vanzo3, Klemen Lisjak3

1University of Nova Gorica, Wine Research Centre, Glavni trg 8, 5271 Vipava, Slovenia
2University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, 1000 Ljubljana, Slovenia
3Agricultural Institute of Slovenia, Central Laboratories, Hacquetova ulica 17, 1000 Ljubljana, Sloveni

Contact the author*

Keywords

non-Saccharomyces yeasts, volatile thiols, esters, sensory properties

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

POTENTIAL DEACIDIFYING ROLE OF A COMMERCIAL CHITOSAN: IMPACT ON PH, TITRATABLE ACIDITY, AND ORGANIC ACIDS IN MODEL SOLUTIONS AND WHITE WINE

Chitin is the main structural component of a large number of organisms (i.e., mollusks, insects, crustaceans, fungi, algae), and marine invertebrates including crabs and shrimps. The main derivative of chitin is chitosan (CH), produced by N-deacetylation of chitin in alkaline solutions. Over the past decade, the OIV/OENO 338A/ 2009 resolution approved the addition of allergen-free fungoid CH to must and wine as an adjuvant for microbiological control, prevention of haziness, metals chelation and ochratoxins removal (European Commission. 2011). Despite several studies on application of CH in winemaking, there are still very limited and controversial data on its interaction with acidic components in wine (Colan-gelo et al., 2018; Castro Marin et al., 2021).

INSIGHT THE IMPACT OF GRAPE PRESSING ON MUST COMPOSITION

The pre-fermentative steps play a relevant role for the characteristics of white wine [1]. In particular, the grape pressing can affect the chemical composition and sensory profile and its optimized management leads to the desired extraction of aromas and their precursors, and phenols resulting in a balanced wine [2-4]. These aspects are important especially for must addressed to the sparkling wine as appropriate extraction of phenols is expected being dependent to grape composition, as well.

DETERMINATION OF FREE AMINO ACIDS, AMINO ACID POTENTIAL AND PROTEASE ACTIVITY IN THE LEES AND STILL WINES OF CHAMPAGNE

Prior to winemaking, organic or mineral nitrogen compound concentrations are usually measured in the vineyard and in grape musts. These indicators facilitate vine cultivation decisions, usually through yield or vigor. During vinification, yeast and bacteria metabolize nitrogen compounds in the musts in order to generate biomass. After fermentation, the microorganisms rerelease a part of this nitrogen as soluble compounds into the wines. Another part remains bound in the lees and can be lost during racking. The must’s natural nitrogen quantities, additional supplements during fermentation, and lees contact management enhance the release of nitrogen compounds to the wines. During ageing these nitrogen compounds – primarily the amino acids – are implicated in the generation of odorous compounds such as heterocycles(1).

NOVEL BENZENETHIOLS WITH PHENOLS CAUSE ASHY, SMOKE FLAVOR PERCEPTION IN RED WINES

Smoke impacts on wines are becoming a worldwide problem; the size and severity of wildfires increasing due to influences from changing climates.¹ For over a century, wines have been known to have a unique issue of absorbing chemical compounds derived from wildfire smoke wherein the flavor of the subsequent wine becomes ashy, rubbery, campfire-like, and smoky.² The economic impacts of a smoke-impacted wine can last for years depending on the grape varietal, costing Oregon and Washington states in the United States over a billion dollars from the 2020 wildfires, as an example.³ While years of research have indicated elevated concentrations of smoke-related compounds, such as guaiacol and syringol, in wines after smoke events, unfortunately, replicating the sensory experience using smoke-associated phenols has not had much success.⁴

AGING PATTERNS OF VARIETAL VOLATILE PROFILES OF WHITE WINES: A CASE STUDY ON 18 ITALIAN VARIETAL WHITE WINES

During wine aging many compositional changes take place. In particular, aroma undergoes dramatic modifications through a wide range of reactions that to date are only partly understood. Italy owns one of the largest ampelographic heritages worldwide, with over three-hundred different varieties. Among these, many white grapes are employed for the production of dry still white wines. Some of these wines are consumed young while others are more prone to aging. For many of these wines, the aging patterns related to volatile composition are still unknown.