terclim by ICS banner
IVES 9 IVES Conference Series 9 HOW OXYGEN CONSUMPTION INFLUENCES RED WINES VOLTAMMETRIC PROFILE

HOW OXYGEN CONSUMPTION INFLUENCES RED WINES VOLTAMMETRIC PROFILE

Abstract

Phenolic compounds play a central role in sensory characteristics of wine, such as colour, mouthfeel, flavour and determine its shelf life. Furthermore, the major non-enzymatic wine oxidation process is due to the catalytic oxidation of phenols in quinones. Due their importance, during the years have been developed different analytical methods to monitor the concentration of phenols in wine, such as Folin-Ciocalteu method, spectrophotometric techniques and HPLC. These methods can also be used to follow some oxidation-related chemical transformations. However, these methods are complex, expensive and time consuming, thus not affordable for most small winery. Consequently, the development of several techniques that are faster, cheaper and user-friendly are currently of great interest. Among these, voltammetry has shown to be able to discriminate well wines according to their chemical composition, in particular in relationship to phenolic compounds. Aims: Our work aimed to understand the effect of oxygenation on voltammetric signals of wines. Material and Methods: DIfferent commercial red wines purchased from a local stores have been used in this study. The experimental protocol involved oxygenation of the wines in a 1 L bottle by hand shaking until the desired oxygen level was reached. Three level of oxygenation were chosen: 1.5 (TA), 5 (TB) and 7 mg/L O₂ (TC). Measures of oxygen have been carried out with a portable oximeter. The oxygenated wine was placed in 125 ml glass vials fitted with an oxygen sensor, filled without leaving any headspace and closed with sealing wax in order to not have oxygen exchanges with the outside. Electrochimical measurements were performed with a with a potentiostat using disposable screen- printed sensors in a three-electrode arrangement. Total polyphenols, anthocyanins, free and total SO₂ measurements were carried out using a multiparametric analyser and the dedicated kit. For colour de- termination were measured the absorbances at wavelengths 420, 520 and 620. The measures to determine the oxygen kinetic consumption were performed every 24 for hours. At the same time were carried out analysis with the multiparametric analyser and for colour determination. Results: Red wine voltammograms were impacted by oxygenation, with several voltametric features showing variation in profile and peak intensity according to the level of oxygen consumption. Different signal treatments strategies were applied to highlight the regions of the voltammograms mostly affected by oxidation, in particular through the use of derivative voltammetry.

 

1. Makhotkina, O., & Kilmartin, P. A. (2009). Uncovering the influence of antioxidants on polyphenol oxidation in wines using an electrochemical method: Cyclic voltammetry. Journal of Electroanalytical Chemistry, 633(1), 165-174
2. Ugliano, M., Slaghenaufi, D., Picariello, L., & Olivieri, G. (2020). Oxygen and SO₂ consumption of different enological tannins in relationship to their chemical and electrochemical characteristics. Journal of Agricultural and Food Chemistry, 68(47), 13418-13425.
3. Ferreira, C., Sáenz-Navajas, M. P., Carrascón, V., Næs, T., Fernández-Zurbano, P., & Ferreira, V. (2021). An assessment of voltammetry on disposable screen printed electrodes to predict wine chemical composition and oxygen consumption rates. Food Chemistry, 365, 130405.
4. P. A. Kilmartin, Electrochemistry applied to the analysis of wine: A mini-review, Electrochemistry Communications, 2016, 67, 39-42 

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Rosario Pascale, Leonardo Vanzo, Giacomi Cristanelli, Maurizio Uglian

Department of Biotechnology, University of Verona, 37134 Verona, Italy

Contact the author*

Keywords

Red wine, Oxygen, Phenolic compounds, Voltammetry

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

CHARACTERIZATION OF ENOLOGICAL OAK TANNIN EXTRACTS BY MULTI-ANALYTICAL METHODS APPROACH

Oak tannin extracts are commonly used to improve wine properties. The main polyphenols found in oak wood extracts are ellagitannins¹ that release ellagic acid upon hydrolysis and comprise numerous structures². Moreover, oak tannin extracts contain other compounds giving a complex mixture. Consequently, the official OIV method based on gravimetric analysis of the tannin fraction adsorbed on polyvinylpolypyrrolidone is not sufficient to describe their composition and highlight their chemical diversity.

EVALUATION OF A SEAWEED EXTRACT OF RUGULOPTERYX OKAMURAE AGAINST ERYSIPHE NECATOR IN GRAPEVINE

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

WINE WITHOUT ADDED SO₂: OXYGEN IMPACT AND EVOLUTION ON THE POLYPHENOLIC COMPOSITION DURING RED WINE AGING

SO₂ play a major role in the stability and wine during storage. Nowadays, the reduction of chemical input during red winemaking and especially the removing SO₂ is a growing expectation from the consumers. Winemaking without SO₂ is a big challenge for the winemakers since the lack of SO₂ affects directly the wine chemical evolution such as the phenolic compounds as well as its microbiological stability.

IMPACT OF HARVEST DATE ON THE FINE MOLECULAR COMPOSITION OF MUST AND BORDEAUX RED WINE (VAR. MERLOT, CABERNET SAUVIGNON). FOCUS ON ACIDITY AND SENSORY IMPACT AFTER FIVE YEARS OF AGING

Climate change has brought several impacts that are becoming increasingly intense during the last few years and put at risk the quality of the berries or even the plant’s sustainability. Such extreme climatic events impact the composition of the wine while modulating its quality and the consumer preferences (Tempère et al., 2019). The three most important changes that take place in the must are: 1) decrease acidity, 2) increase of the concentration of sugar, hence increase of alcohol in the wine, and 3) modification
of the sensory balance and the development for example of cooked fruit aromas.

EVALUATION OF THE OENOLOGICAL POTENTIAL OF NEW RESISTANT VARIETIES MEETING TYPICAL BORDEAUX CHARACTERISTICS

Varietal innovation is a major lever for meeting the challenges of the agro-ecological transition of vi-neyards and their adaptation to climate change. To date, selection work has already begun in the Bordeaux region through the Newvine project. The aim of this project is to create new vine varieties with resistance to mildew and powdery mildew, adapted to the climatic conditions of the Bordeaux region and enabling the production of wines that are in line with consumer tastes and the expected typicity of Bordeaux wines.