terclim by ICS banner
IVES 9 IVES Conference Series 9 THE POTENTIAL USE OF SOLUBLE POLYSACCHARIDES TO PREVENT THE OXIDATION OF ROSÉ WINES

THE POTENTIAL USE OF SOLUBLE POLYSACCHARIDES TO PREVENT THE OXIDATION OF ROSÉ WINES

Abstract

Lately, rosé wine is rapidly increasing its popularity worldwide. Short-time macerations with the red skin of the grapes cause the partial extraction of anthocyanins, which are responsible for the pinkish-salmon hue of rosé wines. However, the low quantity of tannins (antioxidants) and richness in phenolic acids, which can be easily oxidized into yellowish pigments, tend to predispose rosé wines to an undesirable browning. Although the use of SO₂ for the prevention of oxidation is highly extended, this practice is expected to be reduced. Therefore, the search for alternative oenological adjuvants that prevent the oxidation and browning of rosé wines is highly desired. Thus, the aim of this work is to assess the effect of the addition of soluble polysaccharides, issued from grape pomace on the oxidation process. To do this, rosé wines were made using grapes from V. vinifera cv Syrah and employing two different maceration times: short (S, 10 min) and long (L, 2 hours).

Thus, two different wines were elaborated (SYS and SYL). Soluble polysaccharides were extracted, purified and characterized (by means of HPLC-DAD-MS and HPLC-RID) from white grape pomace and added to the rosé wines. Then, wines were submitted to an oxidation process by reaching oxygen saturation level in the solution. Wines’ phenolic composition was studied before the oxidation process and then its evolution was monitored.

The extract of polysaccharides presented three main fractions: F1 (25%) with a MW of 104 kDa; F2 (13%) with a MW of 8 kDa and F3 (62%) with a MW of 2 kDa. The polysaccharide extract was analysed by HPLC-DAD-MS after acid hydrolysis and a chemical modification reaction, in order to obtain a derivative of the monosaccharide which could be detected by UV. The main constitutive monosaccharide units detected were: galacturonic acid (26.3%), arabinose (26.2%), galactose (16%), xylose (11.4%), glucose (9.0%), mannose (6.6%), rhamnose (3.2%) and glucuronic acid (1.3%).

Two antioxidant test (FRAP and ABTS) were performed on the polysaccharide extract for the purpose of measuring its potential use as an antioxidant. Phenolic composition was analysed by HPLC-DAD-MS during the duration of the study (60 days).

Results allowed us to assess the importance of polysaccharide addition to modify the ability of rosé wines to resist oxidation, evaluating the possible application of a natural polysaccharide obtained from wine’s by-product as an oenological adjuvant.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Puerta-García, Ivan. Dueñas, Montserrat. García-Estévez, Ignacio. Salas, Erika. Escribano-Bailón, Maria-Teresa

Contact the author*

Keywords

rosé wine, polysaccharide, oxidation, phenolic compounds

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

Pre-fermentative skin maceration is a technique used in white wine production to enhance varietal aroma, but it can increase protein concentration, leading to protein instability and haze formation [1]. To prevent protein instability, wine producers typically use fining agents such as bentonite, before wine bottling, which can negatively impact sensory characteristics and produce waste [2,3]. The aim of this study was to understand the impact of alternative techniques such as the application of polysaccharides (k-carrageenan and chitosan) on protein stability and on the wine macromolecular composition.

REVEALING THE ORIGIN OF BORDEAUX WINES WITH RAW 1D-CHROMATOGRAMS

Understanding the composition of wine and how it is influenced by climate or wine-making practices is a challenging issue. Two approaches are typically used to explore this issue. The first approach uses chemical
fingerprints, which require advanced tools such as high-resolution mass spectrometry and multidimensional chromatography. The second approach is the targeted method, which relies on the widely available 1-D GC/MS, but involves integrating the areas under a few peaks which ends up using only a small fraction of the chromatogram.

ASSESSMENT OF GRAPE QUALITY THROUGH THE MONITORING OFPHENOLIC RIPENESS AND THE APPLICATION OF A NEW RAPID METHOD BASED ON RAMAN SPECTROSCOPY

The chemical composition of grape berries at harvest is one of the key aspects influencing wine quality and depends mainly on the ripeness level of grapes. Climate change affects this trait, unbalancing technological and phenolic ripeness, and this further raises the need for a fast determination of the grape maturity in order to quickly and efficiently determine the optimal time for harvesting. To this end, the characterization of variety-specific ripening curves and the development of new and rapid methods for determining grape ripeness are of key importance.

NEUROPROTECTIVE AND ANTI-INFLAMMATORY PROPERTIES OF HYDROXYTYROSOL: A PROMISING BIOACTIVE COMPONENT OF WINE

Hydroxytyrosol (HT) is a phenolic compound present in olives, virgin olive oil and wine. HT has attracted great scientific interest due to its biological activities which have been related with the ortho-dihydroxy conformation in the aromatic ring. In white and red wines, HT has been detected at concentrations ranging from 0.28 to 9.6 mg/L and its occurrence has been closely related with yeast metabolism of aromatic amino acids by Ehrlich pathway during alcoholic fermentation. One of the most promising properties of this compound is the neuroprotective activity against pathological mechanisms related with neurode-generative disorders including Alzheimer’s and Parkinson’s disease.

ESTIMATING THE INITIAL OXYGEN RELEASE (IOR) OF CORK CLOSURES

Many factors influence aging of bottled wine, oxygen transfer through the closure is included. The maximum uptake of wine before oxidation begins varies from 60 mg.L-¹ to 180 mg.L-1 for white and red wines respectively [1].
The process of bottling may lead to considerable amounts of oxygen. The actual contribution of the transfer through the closure system becomes relevant at the bottle storage, but the amounts are small compared to prepacking operations [2] and to the total oxygen attained during filling.