terclim by ICS banner
IVES 9 IVES Conference Series 9 VOLTAMETRIC PROFILING OF RED WINE COMPOSITION DURING MACERATION: A STUDY ON FOUR GRAPE VARIETIES

VOLTAMETRIC PROFILING OF RED WINE COMPOSITION DURING MACERATION: A STUDY ON FOUR GRAPE VARIETIES

Abstract

During red wine vinification, maceration allows the must, and consequently the wine, to be enriched with several compounds that contribute to the creation of the typical organoleptic characteristics of red wines. Among these, extraction of polyphenols (PPs) during maceration is a major process of enological interest.

The purpose of this study was the evaluate the suitability of a rapid analytical approach based in linear sweep voltammetry to monitor PPs extraction during vinification. Four red grape varieties were investigated, and fermentations were carried out with three different yeasts in triplicate. The density was evaluated daily and every 48h samples were taken to monitor changes in voltametric profiles and in the anthocyanins, polyphenols and precipitable tannins content.

The voltametric profiles of the wines were monitored using disposable screen-printed carbon electrodes with the working and counter electrode in carbon paste and an Ag/AgCl reference electrode. A drop of sample was loaded onto the sensor, and linear sweep voltammograms were acquired between 0-1200mV at a scan rate of 100mV/s. Analyzing the voltammograms, it was possible to observe differences between varieties and, within each variety, the temporal evolution of maceration.

In the second-derivative voltammograms, a positive peak was observed at low potentials, it increases initially showing a maximum after 7 days of fermentation. This peak is associated with the more easily oxidized compounds in wine. Another region of the voltammogram that shows a trend associated with the progress of fermentation is that around 440mV; in this region the negative peak reaches a maximum after 24 h after the start of maceration and then slowly decreases. This region has been associated with the concentration of monomeric anthocyanins and flavanols. The negative peak around 780mV results initially influenced by the presence of free SO₂, when SO₂ is bound the peak decreases in intensity and then increases again during maceration. By constructing PLS-R models for the concentration of anthocyanins, polyphenols and precipitable tannins the best pre-processing method resulted the second derivative and good regression models were obtained (R2 from 0.75 to 0.95).

In conclusion, this study provides a first proof of concept of the suitability of a simple analytical approach based on linear sweep voltammetry to monitor the evolution of phenolic composition during red wine maceration.

 

1. P. A. Kilmartin, Electrochemistry applied to the analysis of wine: A mini-review, Electrochemistry Communications, 2016, 67, 39-42
2. M. Ugliano, Rapid fingerprinting of white wine oxidizable fraction and classification of white wines using disposable screen printed sensors and derivative voltammetry, Food Chemistry, 2016, 212, 837-843
3. C. Ferreira, M.P. Sáenz-Navajas, V. Carrascón, T. Næs, P. Fernández-Zurbano, V. Ferreira, An assessment of voltammetry on disposable screen printed electrodes to predict wine chemical composition and oxygen consumption rates, Food Chemistry, 2021, 365, 130405

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Leonardo Vanzo¹, Nicola Dalla Valle¹, Giacomo Cristanelli¹, Davide Slaghenaufi¹, Maurizio Ugliano¹

1. Department of Biotechnology, University of Verona

Contact the author*

Keywords

Maceration, Voltammetry, Polyphenols, Red wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

AROMA ASSESSMENT OF COMMERCIAL SFORZATO DI VALTELLINA WINES BYINSTRUMENTAL AND SENSORY METHODOLOGIES

Sforzato di Valtellina DOCG is a special dry red wine produced from partially dehydrated Nebbiolo wine-grapes growing in the Rhaetian Alps valley of Valtellina (Lombardy, Italy). Valtellina terraced vineyards are located at an altitude of 350–800 m according to ‘heroic’ viticulture on steep slopes. The harvested grape bunches are naturally dehydrated indoors, where a slow and continuous withering occurs (about 20% w/w of weight loss), until at least 1st December when the grapes reach the desired sugar content and can be processed following a normal winemaking with maceration.

INVESTIGATION OF FILM COATINGS AS A PROTECTIVE LAYER IN REDUCING THE ABSORPTION OF SMOKE PHENOLS INTO PINOT NOIR GRAPES

Wine grapes exposed to wildfire smoke have resulted in wines with burnt and ashy sensory characteristics¹, that are undesirable qualities in wine. In extreme wildfire events, this can lead to total loss of grape crop. Currently there are no effective solutions in the market to prevent the uptake of smoke compounds into grapes. In this study, previously developed innovative film coatings were tested to analyze their effectiveness in reducing smoke phenol absorption². Four different cellulose nanofiber-based film types were investigated.

EFFECT OF MANNOPROTEIN-RICH EXTRACTS FROM WINE LEES ON PHENOLICCOMPOSITION AND COLOUR OF RED WINE

In 2022, wine production was estimated at around 260 million hl. This high production rate implies to generate a large amount of by-products, which include grape pomace, grape stalks and wine lees. It is estimated that processing 100 tons of grapes leads to ~ 22 tons of by-products from which ~ 6 tons are lees [1]. Wine lees are a sludge-looking material mostly made of dead and living yeast cells, yeast debris and other particles that precipitate at the bottom of wine tanks after alcoholic fermentation. Unlike grape pomace or grape stalks, few strategies have been proposed for the recovery and valorisation of wine less [2].

ABOUT THE ROLE PLAYED BY THE DIFFERENT POLYPHENOLS ON OXYGEN CONSUMPTION AND ON THE ACCUMULATION OF ACETALDEHYDE ANDSTRECKER ALDEHYDES DURING WINE OXIDATION

In a previous work1, it was suggested that the different contents in delphinidin and catechin of the grapes were determinant on the O2 consumption and Strecker aldehyde (SAs) accumulation rates. Higher delphinidin seemed to be related to a faster O2 consumption and a smaller SAs accumulation rate, and the opposite was observed regarding catechin.
In the present paper, these observations were fully corroborated by adding synthetic delphinidin to a wine model containing polyphenolic fractions (PFs) extracted from garnacha and synthetic catechin to a wine model containing PF extracted from tempranillo: The delphinin-containing garnacha model consumed O₂ significantly faster and accumulated significantly smaller amounts of SAs than the original garnacha model, and the catechin-containing tempranillo model, consumed O2 significantly slower and accumulated significantly higher amounts of SAs than the original tempranillo model.

HYDROXYTYROSOL PRODUCTION BY DIFFERENT YEAST STRAINS: SACCHAROMYCES AND NON-SACCHAROMYCES AND THE RELATION WITH THE NITROGEN CONSUMPTION

Hydroxytyrosol (HT) is a phenolic compound with extensive bioactive properties. It is present in olives, olive oil and wines. Its occurrence in wines is partly due to yeast synthetise tyrosol from tyrosine by the Ehrlich pathway, which is subsequently hydroxylated to .
The aim of the present work is to study how different yeast strains can influence in the HT production and, how the different nitrogen consumption of each strain can interfere the production of bioactive compounds.