terclim by ICS banner
IVES 9 IVES Conference Series 9 VOLTAMETRIC PROFILING OF RED WINE COMPOSITION DURING MACERATION: A STUDY ON FOUR GRAPE VARIETIES

VOLTAMETRIC PROFILING OF RED WINE COMPOSITION DURING MACERATION: A STUDY ON FOUR GRAPE VARIETIES

Abstract

During red wine vinification, maceration allows the must, and consequently the wine, to be enriched with several compounds that contribute to the creation of the typical organoleptic characteristics of red wines. Among these, extraction of polyphenols (PPs) during maceration is a major process of enological interest.

The purpose of this study was the evaluate the suitability of a rapid analytical approach based in linear sweep voltammetry to monitor PPs extraction during vinification. Four red grape varieties were investigated, and fermentations were carried out with three different yeasts in triplicate. The density was evaluated daily and every 48h samples were taken to monitor changes in voltametric profiles and in the anthocyanins, polyphenols and precipitable tannins content.

The voltametric profiles of the wines were monitored using disposable screen-printed carbon electrodes with the working and counter electrode in carbon paste and an Ag/AgCl reference electrode. A drop of sample was loaded onto the sensor, and linear sweep voltammograms were acquired between 0-1200mV at a scan rate of 100mV/s. Analyzing the voltammograms, it was possible to observe differences between varieties and, within each variety, the temporal evolution of maceration.

In the second-derivative voltammograms, a positive peak was observed at low potentials, it increases initially showing a maximum after 7 days of fermentation. This peak is associated with the more easily oxidized compounds in wine. Another region of the voltammogram that shows a trend associated with the progress of fermentation is that around 440mV; in this region the negative peak reaches a maximum after 24 h after the start of maceration and then slowly decreases. This region has been associated with the concentration of monomeric anthocyanins and flavanols. The negative peak around 780mV results initially influenced by the presence of free SO₂, when SO₂ is bound the peak decreases in intensity and then increases again during maceration. By constructing PLS-R models for the concentration of anthocyanins, polyphenols and precipitable tannins the best pre-processing method resulted the second derivative and good regression models were obtained (R2 from 0.75 to 0.95).

In conclusion, this study provides a first proof of concept of the suitability of a simple analytical approach based on linear sweep voltammetry to monitor the evolution of phenolic composition during red wine maceration.

 

1. P. A. Kilmartin, Electrochemistry applied to the analysis of wine: A mini-review, Electrochemistry Communications, 2016, 67, 39-42
2. M. Ugliano, Rapid fingerprinting of white wine oxidizable fraction and classification of white wines using disposable screen printed sensors and derivative voltammetry, Food Chemistry, 2016, 212, 837-843
3. C. Ferreira, M.P. Sáenz-Navajas, V. Carrascón, T. Næs, P. Fernández-Zurbano, V. Ferreira, An assessment of voltammetry on disposable screen printed electrodes to predict wine chemical composition and oxygen consumption rates, Food Chemistry, 2021, 365, 130405

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Leonardo Vanzo¹, Nicola Dalla Valle¹, Giacomo Cristanelli¹, Davide Slaghenaufi¹, Maurizio Ugliano¹

1. Department of Biotechnology, University of Verona

Contact the author*

Keywords

Maceration, Voltammetry, Polyphenols, Red wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

NEW PLANT BIOPOLYMERS FOR THE COLLOIDAL STABILITY OF THE COLORING MATTER OF RED WINES

The color as well as the “clarity” of red wines are ones of the qualities required by the consumers. Red wines must have colloidal stability from its bottling to its consumption. The supplementation of red wines with additives, and especially Acacia senegal gum, contributes to its organoleptic properties such as the colloidal stabilization of the coloring matter. In a global perspective of limitation of additives in the field of enology, one of the objectives is notably (i) to reduce the use of additives in wines, by their number and/or their quantity, and (ii) to favor the use of natural additives while preserving the organoleptic and sensory qualities of wines.

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease.

ALCOHOLIC FERMENTATION AND COLOR OF ROSÉ WINES: INVESTIGATIONS ON THE MECHANISMS RESPONSIBLE FOR SUCH DIVERSITY

Color is one of the key elements for the marketing of rosé wines due to their packaging in transparent bottles. Their broad color range is due to the presence of pigments belonging to phenolic compounds extracted from grapes or formed during the wine-making process. However, the mechanisms responsible for such diversity are poorly understood. The few investigations performed on rosé wines showed that their phenolic composition is highly variable, close to that of red wines for the darkest rosés but very different for light ones [1]. Moreover, large variations in the extent of color loss taking place during fermentation have been reported but the mechanisms involved and causes of such variability are unknown.

ACCUMULATION OF GRAPE METABOLITES IS DIFFERENTLY IMPACTED BY WATER DEFICIT AT THE BERRY AND PLANT LEVELS IN NEW FUNGUS DISEASE-TOLERANT GENOTYPES

The use of new fungus disease-tolerant varieties is a promising long-term solution to better manage chemical input in viticulture, but unfortunately little is known regarding these new hybrids fruit development and metabolites accumulation in front of abiotic stresses such as water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD.

WINE AS AN EMOTIONAL AND AESTHETIC OBJECT: IMPACT OF EXPERTISE

Wine tasting has been shown to provide emotions to tasters (Coste et al. 2018). How will expertise impact this emotional response? Burnham and Skilleås (2012) reported that the cultural, experiential, and aesthetic competencies characterize an expert in wine compared to a novice. Although there is no consensual definition of an aesthetic experience, Burnham and Skilleås (2012) reported that aesthetic appreciation is “disinterested, normative for others and communicable” in comparison to sensory pleasure.