Macrowine 2021
IVES 9 IVES Conference Series 9 Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Abstract

Condensed tannin is a principle group of polyphenol compounds derived from grape, greatly contributing to the bioactivity and the sensory perception of wine. Condensed tannins present as a heterogeneous mixture in nature involving various degrees of both polymerization and galloylation. Even though multiple attempts focusing on fractionation of grape condensed tannins by solid-phase have been conducted over the past decades, few individual tannins have been purified and identified. Hence, our knowledge on grape and wine condensed tannin moleculars has to be limited at the several known monomeric, dimeric and trimeric proanthocyanidins. In the present study, a rapid and effective approach was developed to isolate the galloylated proanthocyanidin from the non-galloylated forms in grape seed tannins and fractionate the non-galloylated proanthocyanidin according to their degree of polymerization by the technique of Centrifugal partition chromatography (CPC). The application of CPC on grape tannins fractionation is efficient, less-time consuming, less expensive and with higher recovery. More importantly, it could effectively isolate galloylated tannins at the beginning and eliminate its interference in the other fractions. After fractionation performed by the technique of CPC, the repurification was achieved by either preparative hydrophilic interaction chromatography or reversed phase high-performance liquid chromatography (HPLC). Eventually, a series of individual condensed tannins with certain degree of polymerization and galloylation from grape were isolated successively. Analyte identity and purity were examined by reversed-phase UPLC-DAD-ESI-Q-TOF and normal-phase HPLC-UV-Fluo-MS, respectively. The astringency perception contributions of the purified individual tannins were examined by the approach of salivary binding ability test. The results were observed by HPLC-Fluo and quantified by the difference of the amount of proanthocyanidins between before and after interaction.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Wen Ma*, Hua Li, Michael Jourdes, Pierre Waffo-Téguo, Pierre-Louis Teissedre

*ISVV

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

Use of computational modelling for selecting adsorbents for improved fining of wine

The occurrence of faults and taints in wine, such as those caused by microbial spoilage or various taints, have resulted in significant financial losses to wine producers. The wine industry commits significant financial resources towards fining and taint removal processes each year. Fining involves the addition of one or more adsorptive substrates to juice or wine to bind certain components, thus reducing their concentration [1]. However, these processes are often not selective and can also remove desirable flavour and aroma compounds.

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Correlations between N,S,O-heterocycle levels and age of Champagne base wines

Champagne regulation allows winegrowers to stock small amounts of still wines in order to compensate vintages’ quality shifts mainly due to climate variations. According to their technical requirements and house style some Champagne producers (commonly named “Champagne houses”) use these stored wines in the blend in order to introduce an element of complexity. These wines possess the particularity of being aged on fine lees in thermo-regulated stainless steel tanks. The Champagne house of Veuve Clicquot Ponsardin has several wines stored this way.