Macrowine 2021
IVES 9 IVES Conference Series 9 Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Abstract

Condensed tannin is a principle group of polyphenol compounds derived from grape, greatly contributing to the bioactivity and the sensory perception of wine. Condensed tannins present as a heterogeneous mixture in nature involving various degrees of both polymerization and galloylation. Even though multiple attempts focusing on fractionation of grape condensed tannins by solid-phase have been conducted over the past decades, few individual tannins have been purified and identified. Hence, our knowledge on grape and wine condensed tannin moleculars has to be limited at the several known monomeric, dimeric and trimeric proanthocyanidins. In the present study, a rapid and effective approach was developed to isolate the galloylated proanthocyanidin from the non-galloylated forms in grape seed tannins and fractionate the non-galloylated proanthocyanidin according to their degree of polymerization by the technique of Centrifugal partition chromatography (CPC). The application of CPC on grape tannins fractionation is efficient, less-time consuming, less expensive and with higher recovery. More importantly, it could effectively isolate galloylated tannins at the beginning and eliminate its interference in the other fractions. After fractionation performed by the technique of CPC, the repurification was achieved by either preparative hydrophilic interaction chromatography or reversed phase high-performance liquid chromatography (HPLC). Eventually, a series of individual condensed tannins with certain degree of polymerization and galloylation from grape were isolated successively. Analyte identity and purity were examined by reversed-phase UPLC-DAD-ESI-Q-TOF and normal-phase HPLC-UV-Fluo-MS, respectively. The astringency perception contributions of the purified individual tannins were examined by the approach of salivary binding ability test. The results were observed by HPLC-Fluo and quantified by the difference of the amount of proanthocyanidins between before and after interaction.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Wen Ma*, Hua Li, Michael Jourdes, Pierre Waffo-Téguo, Pierre-Louis Teissedre

*ISVV

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Application of high power ultrasounds during red wine vinification

Wine color is one of the main organoleptic characteristics influencing its quality. It is of especial interest in red vinifications due to the economic resources that wineries have to invest for the extraction of the phenolic compounds responsible of wine color, compounds that are mainly located inside the skin cell vacuoles. Moreover, these phenolic compounds not only influence color but also other organoleptic properties such as body, mouthfeel, astringency and flavour. The transference of phenolic compounds from grapes to must during vinification is closely related with the type of grapes and the winemaking technique.

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method.