terclim by ICS banner
IVES 9 IVES Conference Series 9 Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Abstract

Carbaryl is an acetylcholine esterase inhibitor-type insecticide used for pest control on grapevine. We repeatedly observed the occurrence of interveinal leaf necrosis following carbaryl spray application in a Vitis rupestris x Vitis riparia F1 hybrid progeny vineyard.  Spray applications induced necrosis in this progeny under both Missouri and New York field conditions an approximate one-to-one sensitive-to-insensitive segregation ratio and with 42% concordance. Results of subsequent in vitro experiments established causality between carbaryl treatment and leaf necrosis and confirmed the pattern of segregation observed in the field. We consistently map this phenotype to a major QTL on chromosome 16 of the female parent V. rupestris ‘B38’ regardless of whether we used field or in vitro-generated phenotype data. The PN40024 12x.v1 genome sequence under the QTL peak is a gene-rich region encoding several receptor-like kinases and nucleotide-binding leucine-rich repeat receptors. RNA-seq and qPCR analyses of the carbaryl-induced transcriptome demonstrated the up-regulation of genes encoding the immune response regulator EDS1, pathogenesis-related proteins and stilbene synthases in sensitive, but not in insensitive progeny plants. While the development of leaf necrosis involved certain components of pathogen-triggered cell death regulatory pathway, other molecular events did not agree with the “misguided immune response” paradigm. An extensive screen of native North American grapevine accessions suggested that carbaryl sensitivity is rare in Vitis, and possibly unique to the V. rupestris ‘B38’ genotype, though members of Parthenocissus, another Vitaceae genus, are damaged by carbaryl.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Laszlo Kovacs1*, Courteny Coleman1, Courtney Duncan1, Michael Bigelow1, Cody Pham1, Zachary Harris2, Jason Londo3

1 Department of Biology, Missouri State University, Springfield, MO USA
2 Donald Danforth Plant Science Center, St. Louis, MO USA
3 School of Integrative Plant Science, Cornell University, Geneva, NY USA

Contact the author*

Keywords

Insecticide damage, Vitis rupestris ‘B38’, leaf necrosis, immune response, quantitative trait locus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Determination of target compounds in cava quality using liquid chromatography. Application of chemometric tools in data analysis

According to the Protected Designation of Origin (PDO), Cava is protected in the quality sparkling wines made by the traditional Champenoise method were the wine realize a second fermentation inside the own bottle1. Geographical and human peculiarities of each bottle are the main way for the final quality2. The aim of this study is to find correlations and which target compounds are the most representative of the quality of two different grape varieties, Pinot Noir and Xarel·lo. The quality of these two types of grapes is being studied for each variety by a previous classification of the vineyard made by the company who provided the samples (qualities A,B,C,D, being A the better one and D the worst one). The target compounds studied are organic acids and polyphenols. The methodology for the determination of organic acids is HPLC-UV/vis and for some of them the enzymatic methodology.

NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

Wine phenolic compounds are important secondary metabolites in enology due to their antioxidant and nutraceutical properties, and their role in the development of color, taste, and protection of wine from oxidation and spoilage. Tannins are valuable phenolic compounds that contribute significantly to these wine properties, especially in mouthfeel characteristics; however, tannin determination remains a significant challenge, with manual and time-consuming methods or complex methodologies. The purpose of this study is to propose a novel method for quantifying condensed tannins in finished wine products.

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept.

Exploring diversity of grapevine responses to Flavescence dorée infection

Flavescence dorée, a serious threat to grapevine cultivation in several European Countries, is caused by phytoplasmas in the 16Sr-V ribosomal group, classified as quarantine organisms in the EU and transmitted mainly by the insect vector Scaphoideus titanus. The disease is controlled only by indirect and preventive measures, with important economic and environmental concerns. Genetic resources from the great variety of Vitis vinifera germplasm together with application of new genomic techniques could be applied to produce resistant/tolerant plants, once the genetic bases of susceptibility are elucidated. In a current Italian project (BIORES*) we are evaluating different international and local grapevine cvs. as well as microvine plants for their response to FD transmission and multiplication in controlled conditions.

Impact of grape ripening and post-harvest withering on must composition and fermentation kinetics

Postharvest dehydration is a widely employed technique in winemaking to enhance sugar concentration and secondary metabolites from grapes. Different grape varieties exhibit varying responses in terms of dehydration rate and the resulting chemical composition.