terclim by ICS banner
IVES 9 IVES Conference Series 9 Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Abstract

Carbaryl is an acetylcholine esterase inhibitor-type insecticide used for pest control on grapevine. We repeatedly observed the occurrence of interveinal leaf necrosis following carbaryl spray application in a Vitis rupestris x Vitis riparia F1 hybrid progeny vineyard.  Spray applications induced necrosis in this progeny under both Missouri and New York field conditions an approximate one-to-one sensitive-to-insensitive segregation ratio and with 42% concordance. Results of subsequent in vitro experiments established causality between carbaryl treatment and leaf necrosis and confirmed the pattern of segregation observed in the field. We consistently map this phenotype to a major QTL on chromosome 16 of the female parent V. rupestris ‘B38’ regardless of whether we used field or in vitro-generated phenotype data. The PN40024 12x.v1 genome sequence under the QTL peak is a gene-rich region encoding several receptor-like kinases and nucleotide-binding leucine-rich repeat receptors. RNA-seq and qPCR analyses of the carbaryl-induced transcriptome demonstrated the up-regulation of genes encoding the immune response regulator EDS1, pathogenesis-related proteins and stilbene synthases in sensitive, but not in insensitive progeny plants. While the development of leaf necrosis involved certain components of pathogen-triggered cell death regulatory pathway, other molecular events did not agree with the “misguided immune response” paradigm. An extensive screen of native North American grapevine accessions suggested that carbaryl sensitivity is rare in Vitis, and possibly unique to the V. rupestris ‘B38’ genotype, though members of Parthenocissus, another Vitaceae genus, are damaged by carbaryl.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Laszlo Kovacs1*, Courteny Coleman1, Courtney Duncan1, Michael Bigelow1, Cody Pham1, Zachary Harris2, Jason Londo3

1 Department of Biology, Missouri State University, Springfield, MO USA
2 Donald Danforth Plant Science Center, St. Louis, MO USA
3 School of Integrative Plant Science, Cornell University, Geneva, NY USA

Contact the author*

Keywords

Insecticide damage, Vitis rupestris ‘B38’, leaf necrosis, immune response, quantitative trait locus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Setting up new tools to reduce the duration of the grapevine breeding process : Mercier experience

Since some years, the French wine sector faces strategical challenges, all linked to climate change. Multiple issues have been observed like diseases development, early frost, drought, change in the precocity and maturity of grapes, each one resulting in loss of productivity and yield. In France, the varieties proposed today by nurseries are historical varieties that are not well adapted to those changes. Therefore, Mercier Frères, one of the leading grapevine nursery, has decided to start its own research programs, with the help of its laboratory Novatech, to answer the growing demand for new grapevine varieties.

Identification of important genomic regions controlling resistance to biotic and abiotic stresses in Vitis sp. through QTL meta-analysis

In the context of global change, the environmental conditions are expected to be more stressful for viticulture. The choice of the rootstock may play a crucial role to improve the adaptation of viticulture to new biotic and abiotic threats (Ollat et al., 2016). However, the selection of interesting traits in rootstock breeding programs is complex because of the combination of multiple targets in a same ideotype. In this sense, the integration of studies about the genetic architecture for desired biotic and abiotic response traits allow us to identify genomic regions to combine and those with interesting pleiotropic effects.

Development of a LC-FTMS method to quantify natural sweeteners in red wines

The quality of a wine is largely related to the balance between its sourness, bitterness and sweetness. Recently, molecules coming from grapes have been showed to notably contribute to sweet taste of dry wines. To study the viticultural and oenological parameters likely to affect their concentration, their quantification appears of high interest and subsequently requires powerful analytical techniques. Therefore, a new method using liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) was developed and validated to quantify epi-DPA-3′-O-β-glucopyranoside acid (epi-DPA-G) and astilbin, sweet molecules identified in wine. Three gradients were tested on five different C18 columns (Hypersil Gold, HSS T3, BEH, Syncronis and Kinetex).

The regulation of ABA-induced anthocyanin accumulation in grape berry

Color is a key quality trait for grape berry and the producing wines. Berry color of red genotypes is mainly determined by the quantity and composition of anthocyanins accumulated in the skin and/or pulp. Both genetic and environmental factors could influence the quantity and composition of anthocyanins, while the underlying mechanisms are not fully clear. To explore the mechanisms underlying the diversity of anthocyanin accumulation in grape berry, we compared two grapevine genotypes showing distinct sensitivities to ABA-induced anthocyanin biosynthesis, where one genotype showed minor responses to exogenous ABA application while the other showed significant increase in anthocyanins after exogenous ABA application.

Influence of the “terroir” (soil, climate and wine grower) on the quality of red Grenache wines in the Rhône Valley

«L’Observatoire Grenache» est un réseau de parcelles qui a été mis en place par l’Institut Rhodanien en Vallée du Rhône sur les millésimes de 1995 à 1999. Composé de 24 parcelles de Vitis vinifera L. cv Grenache noir, ce réseau vise à étudier l’influence du terroir (sol, climat et vigneron) sur la qualité des vins. Les parcelles ont été choisies afin de représenter différentes situations géographiques et géopédologiques de la vallée du Rhône. Le matériel végétal (clone, porte-greffe), la taille (cordon de Royat), la densité et l’âge de la parcelle ont été encadrées. Ainsi les conditions de milieu (sol, climat) et les pratiques du vigneron étaient les principales sources de variations.