terclim by ICS banner
IVES 9 IVES Conference Series 9 Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Abstract

Carbaryl is an acetylcholine esterase inhibitor-type insecticide used for pest control on grapevine. We repeatedly observed the occurrence of interveinal leaf necrosis following carbaryl spray application in a Vitis rupestris x Vitis riparia F1 hybrid progeny vineyard.  Spray applications induced necrosis in this progeny under both Missouri and New York field conditions an approximate one-to-one sensitive-to-insensitive segregation ratio and with 42% concordance. Results of subsequent in vitro experiments established causality between carbaryl treatment and leaf necrosis and confirmed the pattern of segregation observed in the field. We consistently map this phenotype to a major QTL on chromosome 16 of the female parent V. rupestris ‘B38’ regardless of whether we used field or in vitro-generated phenotype data. The PN40024 12x.v1 genome sequence under the QTL peak is a gene-rich region encoding several receptor-like kinases and nucleotide-binding leucine-rich repeat receptors. RNA-seq and qPCR analyses of the carbaryl-induced transcriptome demonstrated the up-regulation of genes encoding the immune response regulator EDS1, pathogenesis-related proteins and stilbene synthases in sensitive, but not in insensitive progeny plants. While the development of leaf necrosis involved certain components of pathogen-triggered cell death regulatory pathway, other molecular events did not agree with the “misguided immune response” paradigm. An extensive screen of native North American grapevine accessions suggested that carbaryl sensitivity is rare in Vitis, and possibly unique to the V. rupestris ‘B38’ genotype, though members of Parthenocissus, another Vitaceae genus, are damaged by carbaryl.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Laszlo Kovacs1*, Courteny Coleman1, Courtney Duncan1, Michael Bigelow1, Cody Pham1, Zachary Harris2, Jason Londo3

1 Department of Biology, Missouri State University, Springfield, MO USA
2 Donald Danforth Plant Science Center, St. Louis, MO USA
3 School of Integrative Plant Science, Cornell University, Geneva, NY USA

Contact the author*

Keywords

Insecticide damage, Vitis rupestris ‘B38’, leaf necrosis, immune response, quantitative trait locus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Variability of Tempranillo grape quality within the Ribera del Duero do (Spain) and relationships with climatic characteristics

The aim of this research was to evaluate the variability of ripening characteristics of the Tempranillo variety within the Ribera del Duero Designation of Origin (Spain) and it relationships with soil characteristics

The effect of ozonated water treatment on the metabolic profile and resistance of vines to Downy and powdery mildew 

Ozone is a potent oxidizing compound that quickly decomposes into oxygen without residues. Previous works reported that ozone is not only a disinfectant that directly harms the pathogens of the vine but also activates systemic defense systems in the plant by activating oxidative stress. We assume these systemic defense mechanisms are essential to the vines’ resistance to downy and powdery mildew (Plasmopara viticola & Erysiphe necator, respectively). The goals of the research are to examine the effect of spraying with ozone water on the plant’s resistance against the mentioned pathogens as well as to characterize the metabolic profile of the plants treated with ozone as well as physiological characteristics in the vines such as the level of Photosynthesis and crop yield. Vines in the vineyard sprayed with ozone water at concentrations of 2 and 4 PPM weekly and biweekly, untreated control & conventional spray. Leaves were taken from vines 2,4,7,9 and 11 days after exposure to ozone and inoculated with the pathogens.

“Vinhos de mesa” et oenophilie : quand les caractéristiques organoleptiques des cépages américains empêchent l’intégration des consommateurs à l’univers de l’appréciation esthétique

Au Brésil, 80 % du vignoble national et 90 % du vignoble de l’État du Rio Grande do Sul (principale région productrice de vins dans le pays) sont plantés avec des cépages issus de vitis labrusca ou de cépages hybrides (DEBASTIANI, 2015). Une partie de cette production est utilisée pour la préparation de jus de raisin et de concentrés de moût ou de pulpe de raisin. Le restant est consacré à

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.