terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Abstract

Downy mildew, caused by the oomycete Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni, is one of the most destructive grapevine diseases mostly affecting Vitis vinifera L. and impacting on viticulture. The pathogen invasion can induce in grapevine multiple defense reactions, first PAMP-Triggered Immunity and secondly Effector-Triggered Immunity. Plasmopara viticola can overcome these defense mechanisms through the secretion of effectors, such as RxLR, into the plant cells, making it easier for the oomycete to infect grapevines.  Currently, the use of chemical pesticides remains the most effective way to control the pathogen with severe negative side effects on the environment and animal health. Consequently, great attention has been recently paid to identifying new Biological Control Agents (BCAs). At CREA Viticulture and Enology, 47 bacterial isolates were collected from the leaves of three Vitis vinifera cultivars, Blush, Dawn seedless and Argentina, showing different degrees of tolerance to Plasmopara viticola. Three of the collected bacteria, that previous tests suggested as potentially good Plasmopara viticolaantagonists, were sprayed on Cabernet sauvignon leaves at 107 CFU ml-1 48 hours before leaf infection with Plasmopara viticola at 106 sporangia ml-1. A gene expression analysis of Plasmopara viticola effectors PvRxLR28 and PvRxLR67, performed through quantitative PCR, revealed an impairment in the expression levels of the two genes in treated leaves compared with control leaves. These results suggest these bacteria as potential BCAs against Plasmopara viticola. Further transcriptomic analysis will be performed to investigate bacterial effects on the expression of multiple Plasmopara viticola pathogenicity genes.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Camilla Mandorino1,2*, Antonella Salerno1,4, Annalisa Prencipe2, Marco Vendemia1, Flavia Angela Maria Maggiolini1, Maria Francesca Cardone1, Andreia Figueiredo3, Antonio Domenico Marsico1, Carlo Pazzani2

1 CREA – Viticulture and Enology, Turi, Italy
2Department of Biosciences, Biotechnology and Environment, University of Bari “A. Moro”, Bari, Italy
3 Biosystems and Integrative Sciences Institute (BioISI), Plant Biology Department, Science Faculty of Lisbon University, Lisbon, Portugal
4 Department of Soil, Plant and Food Science, University of Bari “A. Moro”, Bari, Italy

Contact the author*

Keywords

native vineyard bacteria, biological control agents, Plasmopara viticola, Vitis vinifera, RxLR effectors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Desorption of phenolic compounds bound to lees by combining hydrolytic enzymes and ultrasounds

he final concentration of phenolic compounds in the wines is usually lower than what might be expected given the phenolic concentration measured in grapes

Read More

Vintage influence on Grenache N, Syrah N and Mourvedre N in Côtes du Rhône (France)

Vintage is part of « terroir ». The aim of this work is to study, through vine and berry parameters, the effect of vintage on the three major red grape varieties in Côtes du Rhône : Grenache N, Syrah N and Mourvedre N. We first characterized vintages 1997 to 2003, highlighting similar features in grape development across the different cultivars since 2001 only.

Read More

Haplotype-resolved genome assemblies of Chasselas and Ugni Blanc

Haplotype-resolved genome assemblies were produced for Chasselas and Ugni Blanc, two heterozygous real-field genetic pool Vitis vinifera cultivars by combining high-fidelity long-read sequencing (HiFi) and high‐throughput chromosome conformation capture (Hi-C). The telomere-to-telomere full coverage of the chromosomes allowed us to assemble separately the two haplo-genomes of both cultivars and revealed structural variations between the two haplotypes of a given cultivar.

Read More

Evolution of chemical pattern related to Valpolicella aroma ‘terroir’ during bottle aging

Valpolicella is a famous Italian wine-producing region. Wines produced in its different sub-regions are believed to be aromatically different, as confirmed by recent studies in our laboratory. Aging is a very common practice in Valpolicella and it is required by the appellation regulation for periods up to four years require wines. The aim of this study was to investigate the evolution, during aging, of volatile chemical composition of Valpolicella wines obtained from grapes harvested in different sub-regions during different vintages.

Read More

Terroir and Typicity: proposed definitions for two essential concepts in the understanding of Geographical Indications and sustainable development

The content of this communication arises from the deliberations of a working group mandated within the framework of the INRA-INAO 2000-2003 research convention, which brought together INAO representatives and researchers who had worked on AOCs or PGIs, in disciplines from the sphere of the humanities (consumer science, marketing, rural development) and biotechnical sciences (agronomy, animal production science, technology, biochemistry).

Read More