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IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Abstract

Downy mildew, caused by the oomycete Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni, is one of the most destructive grapevine diseases mostly affecting Vitis vinifera L. and impacting on viticulture. The pathogen invasion can induce in grapevine multiple defense reactions, first PAMP-Triggered Immunity and secondly Effector-Triggered Immunity. Plasmopara viticola can overcome these defense mechanisms through the secretion of effectors, such as RxLR, into the plant cells, making it easier for the oomycete to infect grapevines.  Currently, the use of chemical pesticides remains the most effective way to control the pathogen with severe negative side effects on the environment and animal health. Consequently, great attention has been recently paid to identifying new Biological Control Agents (BCAs). At CREA Viticulture and Enology, 47 bacterial isolates were collected from the leaves of three Vitis vinifera cultivars, Blush, Dawn seedless and Argentina, showing different degrees of tolerance to Plasmopara viticola. Three of the collected bacteria, that previous tests suggested as potentially good Plasmopara viticolaantagonists, were sprayed on Cabernet sauvignon leaves at 107 CFU ml-1 48 hours before leaf infection with Plasmopara viticola at 106 sporangia ml-1. A gene expression analysis of Plasmopara viticola effectors PvRxLR28 and PvRxLR67, performed through quantitative PCR, revealed an impairment in the expression levels of the two genes in treated leaves compared with control leaves. These results suggest these bacteria as potential BCAs against Plasmopara viticola. Further transcriptomic analysis will be performed to investigate bacterial effects on the expression of multiple Plasmopara viticola pathogenicity genes.

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DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Camilla Mandorino1,2*, Antonella Salerno1,4, Annalisa Prencipe2, Marco Vendemia1, Flavia Angela Maria Maggiolini1, Maria Francesca Cardone1, Andreia Figueiredo3, Antonio Domenico Marsico1, Carlo Pazzani2

1 CREA – Viticulture and Enology, Turi, Italy
2Department of Biosciences, Biotechnology and Environment, University of Bari “A. Moro”, Bari, Italy
3 Biosystems and Integrative Sciences Institute (BioISI), Plant Biology Department, Science Faculty of Lisbon University, Lisbon, Portugal
4 Department of Soil, Plant and Food Science, University of Bari “A. Moro”, Bari, Italy

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Keywords

native vineyard bacteria, biological control agents, Plasmopara viticola, Vitis vinifera, RxLR effectors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

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