terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Abstract

Downy mildew, caused by the oomycete Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni, is one of the most destructive grapevine diseases mostly affecting Vitis vinifera L. and impacting on viticulture. The pathogen invasion can induce in grapevine multiple defense reactions, first PAMP-Triggered Immunity and secondly Effector-Triggered Immunity. Plasmopara viticola can overcome these defense mechanisms through the secretion of effectors, such as RxLR, into the plant cells, making it easier for the oomycete to infect grapevines.  Currently, the use of chemical pesticides remains the most effective way to control the pathogen with severe negative side effects on the environment and animal health. Consequently, great attention has been recently paid to identifying new Biological Control Agents (BCAs). At CREA Viticulture and Enology, 47 bacterial isolates were collected from the leaves of three Vitis vinifera cultivars, Blush, Dawn seedless and Argentina, showing different degrees of tolerance to Plasmopara viticola. Three of the collected bacteria, that previous tests suggested as potentially good Plasmopara viticolaantagonists, were sprayed on Cabernet sauvignon leaves at 107 CFU ml-1 48 hours before leaf infection with Plasmopara viticola at 106 sporangia ml-1. A gene expression analysis of Plasmopara viticola effectors PvRxLR28 and PvRxLR67, performed through quantitative PCR, revealed an impairment in the expression levels of the two genes in treated leaves compared with control leaves. These results suggest these bacteria as potential BCAs against Plasmopara viticola. Further transcriptomic analysis will be performed to investigate bacterial effects on the expression of multiple Plasmopara viticola pathogenicity genes.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Camilla Mandorino1,2*, Antonella Salerno1,4, Annalisa Prencipe2, Marco Vendemia1, Flavia Angela Maria Maggiolini1, Maria Francesca Cardone1, Andreia Figueiredo3, Antonio Domenico Marsico1, Carlo Pazzani2

1 CREA – Viticulture and Enology, Turi, Italy
2Department of Biosciences, Biotechnology and Environment, University of Bari “A. Moro”, Bari, Italy
3 Biosystems and Integrative Sciences Institute (BioISI), Plant Biology Department, Science Faculty of Lisbon University, Lisbon, Portugal
4 Department of Soil, Plant and Food Science, University of Bari “A. Moro”, Bari, Italy

Contact the author*

Keywords

native vineyard bacteria, biological control agents, Plasmopara viticola, Vitis vinifera, RxLR effectors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments.

Read More

Investigation on Valbelluna area and its oenological potentiality: case study on Prosecco DOC

Valbelluna valley is an area located in the northeastern Italy. It is extended from the East-West between Feltre and Belluno, along the Piave waterway and enclosed between Cansiglio valley on the South and the Dolomites in the North. Here, the villages of Limana and Trichiana are present, which are considered for decades potentially interesting areas to aim a niche production with own particular properties.The position of this area, its sun exposition, its soil composition and the microclimate, are ideal factors to obtain vines and consequently wines with unique features especially regarding the diversity and complexity aroma.

Read More

Late pruning as a tool to reduce the risk of spring frosts in a vineyard in Rioja Alavesa (DO Ca Rioja)

The increase in temperature caused by climate change produces an earlier budbreak date that affects the vineyard, which generates a greater risk of damage by spring frosts.

Read More

Three Nebbiolo clone anthocyanin profile as affected by environmental conditions

Vitis vinifera ‘Nebbiolo’ cultivar is a 3’-subsituted anthocyanin prevalent wine variety. It is grown in North-West Italy for the production of high quality ageing wines. In the present work berry skin anthocyanin amounts and profiles of the clones CVT 308, CVT 423 and CVT 142 were studied in 2004 and in 2005 in four environmentally different locations of North-West Italy: Donnas (steep mountain area), Monforte (hilly area, with a pH of 8.1), Vezza (hilly area, with a pH of 8.2) and Lessona (plain area, with a pH of 4.8).

Read More

Combining high-power ultrasound and oenological enzymes during winemaking for improving red wine chromatic characteristics

he use of high-power ultrasound (US) is proving of great interest to the oenological industry due to its effects in the improvement of wine organoleptic characteristics, especially in terms of color [1, 2].

Read More