terclim by ICS banner
IVES 9 IVES Conference Series 9 Spatial variability of grape berry maturation program at the molecular level 

Spatial variability of grape berry maturation program at the molecular level 

Abstract

The application of sensors in viticulture is a fast and efficient method to monitor grapevine vegetative, yield and quality parameters and determine their spatial intra-vineyard variability. Molecular analysis at the gene expression level can further contribute to the understanding of the observed variability by elucidating how pathways responsible for different grape quality traits behave in zones diverging for one or the other parameter. The intra-vineyard variability of a Cabernet Sauvignon vineyard was evaluated by a standard Normalized Difference Vegetation Index (NDVI) mapping approach, employing UAV platform, accompanied by detailed ground-truthing (e.g. vegetative, yield, and berry ripening compositional parameters) that was applied in 14 spots in the vineyard. Berries from different spots were additionally investigated by microarray gene expression analysis, performed at five time points from fruit set to full ripening. The relationships between NDVI and ground measurements were explored by correlation analysis and revealed high variability in the vineyard. Comparison between the transcriptome data of spots with the highest and lowest NDVI values unraveled 968 differentially expressed genes. Among them, were ripening-related genes, found to feature the low vigor spots, and genes involved in photosynthesis mechanisms that were prevalent in the high vigor spots. Spatial variability maps of the expression level of key berry ripening genes showed consistent patterns, aligned with the vineyard vigor map and with spatial maps generated for several vine and berry parameters. These insights suggest that berries from different vigor zones present distinct molecular maturation programs, hence, showing potential in predicting spatial variability in fruit quality.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Ron Shmuleviz1*, Alessandra Amato1, Pietro Previtali2, Elizabeth Green2, Luis Sanchez2, Maria Mar Alsina2, Nick Dokoozlian2, Giovanni Battista Tornielli1,3 and Marianna Fasoli1

1 Department of Biotechnology, University of Verona, 37134 Verona (VR), Italy
2 E. & J. Gallo Winery, Modesto, CA 95354, USA
3 Current address: Department of Agronomy, Food, Natural resources, Animals and  Environment, University of Padova, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

berry ripening, vegetation indices, gene expression analysis, sensors, precision viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

EVALUATION OF A SEAWEED EXTRACT OF RUGULOPTERYX OKAMURAE AGAINST ERYSIPHE NECATOR IN GRAPEVINE

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

Mapping of five loci for black rot resistance in four breeding populations and identification of candidate genes associated with Rgb1

Black rot, caused by Phyllosticta ampelicida (Guignardia bidwellii), is a serious fungal disease in viticulture. Incidences increased in recent years due to reduced pesticide treatments, neglected phytosanitary measurements, and effects of climate change.

Overcoming habit formation in the production of wine

Evidence indicates that climate change affects the environment, human health, and well-being via drought, increasing greenhouse effect, and climatic catastrophes. As the wine sector is also negatively affected by climate change, the role of climate change mitigation and adaptation policies is important in wine production. One example of an adaptation policy is the implementation of grapevine genetics (duchene, 2016), while organic farming may be used as an approach to mitigate the consequences of climate change (vinci et al., 2022). To this end, the european commission’s objective is to reach the european green deal target of at least 25% of the european union’s agricultural land under organic farming by 2030.

Phenolic extraction during fermentation as affected by ripeness level of Syrah/R99 grapes

L’extraction phénolique au cours de la fermentation à partir de vendanges de différents degrees de maturité du cépage Syrah/R99 a été etudiée. Cette travail fait parti d’un projet focalisé sur la qualité du raisin et des vins obtenus au cours du millésime 2002. Les vignes sont situées à Stellenbosch (Afrique du Sud) sur un sol Glenrose

Capture depletion of grapevine DNA: an approach to advance the study of microbial community in wine

The use of next-generation sequencing (NGS) has helped understand microbial genetics in oenology. Current studies mainly focus on barcoded amplicon NGS but not shotgun sequencing, which is useful for functional analyses. Since the high percentage of grapevine DNA conceals the microbial DNA in must, the majority of sequencing data is wasted in bioinformatic analyses. Here we present capture depletion of grapevine whole genome DNA.