terclim by ICS banner
IVES 9 IVES Conference Series 9 Spatial variability of grape berry maturation program at the molecular level 

Spatial variability of grape berry maturation program at the molecular level 

Abstract

The application of sensors in viticulture is a fast and efficient method to monitor grapevine vegetative, yield and quality parameters and determine their spatial intra-vineyard variability. Molecular analysis at the gene expression level can further contribute to the understanding of the observed variability by elucidating how pathways responsible for different grape quality traits behave in zones diverging for one or the other parameter. The intra-vineyard variability of a Cabernet Sauvignon vineyard was evaluated by a standard Normalized Difference Vegetation Index (NDVI) mapping approach, employing UAV platform, accompanied by detailed ground-truthing (e.g. vegetative, yield, and berry ripening compositional parameters) that was applied in 14 spots in the vineyard. Berries from different spots were additionally investigated by microarray gene expression analysis, performed at five time points from fruit set to full ripening. The relationships between NDVI and ground measurements were explored by correlation analysis and revealed high variability in the vineyard. Comparison between the transcriptome data of spots with the highest and lowest NDVI values unraveled 968 differentially expressed genes. Among them, were ripening-related genes, found to feature the low vigor spots, and genes involved in photosynthesis mechanisms that were prevalent in the high vigor spots. Spatial variability maps of the expression level of key berry ripening genes showed consistent patterns, aligned with the vineyard vigor map and with spatial maps generated for several vine and berry parameters. These insights suggest that berries from different vigor zones present distinct molecular maturation programs, hence, showing potential in predicting spatial variability in fruit quality.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Ron Shmuleviz1*, Alessandra Amato1, Pietro Previtali2, Elizabeth Green2, Luis Sanchez2, Maria Mar Alsina2, Nick Dokoozlian2, Giovanni Battista Tornielli1,3 and Marianna Fasoli1

1 Department of Biotechnology, University of Verona, 37134 Verona (VR), Italy
2 E. & J. Gallo Winery, Modesto, CA 95354, USA
3 Current address: Department of Agronomy, Food, Natural resources, Animals and  Environment, University of Padova, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

berry ripening, vegetation indices, gene expression analysis, sensors, precision viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

VOLTAMETRIC PROFILING OF RED WINE COMPOSITION DURING MACERATION: A STUDY ON FOUR GRAPE VARIETIES

During red wine vinification, maceration allows the must, and consequently the wine, to be enriched with several compounds that contribute to the creation of the typical organoleptic characteristics of red wines. Among these, extraction of polyphenols (PPs) during maceration is a major process of enological interest.
The purpose of this study was the evaluate the suitability of a rapid analytical approach based in linear sweep voltammetry to monitor PPs extraction during vinification.

Measurement of grape vine growth for model evaluation

Within a research project for simulating the nitrogen turnover in vineyard soils and the nitrogen uptake by the grape vine, a previously developed plant growth model (Nendel and Kersebaum 2004) had to be evaluated. A dataset was obtained from a monitoring experiment at three vineyard sites with different soil types, conducted in the years 2003 and 2004.

Phenology and maturation of Cabernet Sauvignon grapes from young vineyards at Santa Catarina state, Brazil – a survey of vineyard altitude and mesoclimat influences

Cabernet Sauvignon grapes from recently planted vines in Santa Catarina State (Brazil), were sampled during ripening from the 2005 and 2006 vintages.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

VviSOC1a and VviAG1 act antagonistically in the regulation of flower formation

The SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) is a key floral activator that coordinates external and internal stimuli to ensure timely flowering. During early stages of flower formation, SOC1 represses floral organ identity genes such as AGAMOUS (AG) to prevent premature organ differentiation. In addition to floral organ specification, AG has been shown to regulate fleshy fruit expansion and ripening and, as such, is an important contributor to fruit quality traits. Currently, little is known about the function and gene regulatory network of the grapevine homologs VviSOC1a and VviAG1. As such, the aim of this study was to functionally characterise both genes by overexpressing them in tomato and performing phenotypic and gene expression studies.