terclim by ICS banner
IVES 9 IVES Conference Series 9 Spatial variability of grape berry maturation program at the molecular level 

Spatial variability of grape berry maturation program at the molecular level 

Abstract

The application of sensors in viticulture is a fast and efficient method to monitor grapevine vegetative, yield and quality parameters and determine their spatial intra-vineyard variability. Molecular analysis at the gene expression level can further contribute to the understanding of the observed variability by elucidating how pathways responsible for different grape quality traits behave in zones diverging for one or the other parameter. The intra-vineyard variability of a Cabernet Sauvignon vineyard was evaluated by a standard Normalized Difference Vegetation Index (NDVI) mapping approach, employing UAV platform, accompanied by detailed ground-truthing (e.g. vegetative, yield, and berry ripening compositional parameters) that was applied in 14 spots in the vineyard. Berries from different spots were additionally investigated by microarray gene expression analysis, performed at five time points from fruit set to full ripening. The relationships between NDVI and ground measurements were explored by correlation analysis and revealed high variability in the vineyard. Comparison between the transcriptome data of spots with the highest and lowest NDVI values unraveled 968 differentially expressed genes. Among them, were ripening-related genes, found to feature the low vigor spots, and genes involved in photosynthesis mechanisms that were prevalent in the high vigor spots. Spatial variability maps of the expression level of key berry ripening genes showed consistent patterns, aligned with the vineyard vigor map and with spatial maps generated for several vine and berry parameters. These insights suggest that berries from different vigor zones present distinct molecular maturation programs, hence, showing potential in predicting spatial variability in fruit quality.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Ron Shmuleviz1*, Alessandra Amato1, Pietro Previtali2, Elizabeth Green2, Luis Sanchez2, Maria Mar Alsina2, Nick Dokoozlian2, Giovanni Battista Tornielli1,3 and Marianna Fasoli1

1 Department of Biotechnology, University of Verona, 37134 Verona (VR), Italy
2 E. & J. Gallo Winery, Modesto, CA 95354, USA
3 Current address: Department of Agronomy, Food, Natural resources, Animals and  Environment, University of Padova, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

berry ripening, vegetation indices, gene expression analysis, sensors, precision viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

OmicBots – An innovative and intelligent multi-omics platform facing wine sector challenges

To face emerging competition and challenges, wine producers globally rely on precision viticulture (PV) solutions to boost productivity, enhance quality, increase profitability, and reduce the environmental impact of vineyards. Current pv methods predominantly use multispectral sensor data from several platforms (satellites or vineyard installations). However, these applications generally use data analysis strategies lacking physiological grapevine support.

Bees, climate changes, and “environmental sustainability 4.1c” in viticulture and the territory for a new global multiproductive “biometaethical district 4.1c”

The use of bees as pollinators in vine varieties with physiologically female flowers (Picolit, Bicane, Ceresa, Moscato rosa, etc.) (Cargnello, 1983) and as bio-indicators for biodiversity and environmental sustainability is well-known. Furthermore, there are interests in: 1-a. Making the viticulture of Belluno (Province of Veneto in North-eastern Italy, which is also famous for the Dolomites -a UNESCO World Heritage-) regain the socioeconomic role which it is entitled to and which it had got in its past by aiming at the enhancement of local grape variety in harmony with others, for example with the neighboring area of the Conegliano and Valdobbiadene Prosecco Superiore DOCG; 2-a. Maintaining and further improving the important natural and healthy environment of Belluno, and making its territory and the “lookout” means of the environmental sustainability, including its vineyards, even more naturally original and sustainable 4.1C.

Fungal communites diversity and functional roles of different types of Botrytis cinerea infected grape berries on different growing sites

Botrytis cinerea, an Ascomycota pathogen with a broad host range, infects over 1200 plant species. Grapes infected by this pathogen, which subsequently develop a noble rot, remain in the vineyard for an extended period, thus being exposed to a diverse array of physical, chemical and biological factors, which give rise to a complex microbial community.

Advancement of grape maturity – comparison between contrasting varieties and regions

Grapevine phenology has advanced across many regions, nationally and internationally, in recent decades under the influence of increasing temperatures, resulting in earlier
vintages (Jones and Davis, 2000, Petrie and Sadras, 2008, Tomasi et al., 2011, Webb et al., 2011. Earlier vintages have several ramifications for the wine industry. There are direct implications on quality, due to the fruit ripening during the hotter conditions of summer and early autumn, which then impacts grape composition and wine style (Sadras et al., 2013, Buttrose et al., 1971, Mira de Ordũna, 2010). There are also indirect implications where the fruit is perceived to ripen at a faster rate and the crop reach optimum maturity over a shorter period (Coulter et al., 2016).

Investigation on the potentiality of a biostimulant by Fabaceae tissues and rich in triacontanol to enhance grapevine resilience under drought stress

The primary objective of this research was to investigate the potential benefits of a Fabaceae-based product rich in triacontanol (a long-chain alcohol) applied to Vitis vinifera cv. Merlot, on key physiological and productive parameters of grapevines under controlled water stress conditions.