terclim by ICS banner
IVES 9 IVES Conference Series 9 Spatial variability of grape berry maturation program at the molecular level 

Spatial variability of grape berry maturation program at the molecular level 

Abstract

The application of sensors in viticulture is a fast and efficient method to monitor grapevine vegetative, yield and quality parameters and determine their spatial intra-vineyard variability. Molecular analysis at the gene expression level can further contribute to the understanding of the observed variability by elucidating how pathways responsible for different grape quality traits behave in zones diverging for one or the other parameter. The intra-vineyard variability of a Cabernet Sauvignon vineyard was evaluated by a standard Normalized Difference Vegetation Index (NDVI) mapping approach, employing UAV platform, accompanied by detailed ground-truthing (e.g. vegetative, yield, and berry ripening compositional parameters) that was applied in 14 spots in the vineyard. Berries from different spots were additionally investigated by microarray gene expression analysis, performed at five time points from fruit set to full ripening. The relationships between NDVI and ground measurements were explored by correlation analysis and revealed high variability in the vineyard. Comparison between the transcriptome data of spots with the highest and lowest NDVI values unraveled 968 differentially expressed genes. Among them, were ripening-related genes, found to feature the low vigor spots, and genes involved in photosynthesis mechanisms that were prevalent in the high vigor spots. Spatial variability maps of the expression level of key berry ripening genes showed consistent patterns, aligned with the vineyard vigor map and with spatial maps generated for several vine and berry parameters. These insights suggest that berries from different vigor zones present distinct molecular maturation programs, hence, showing potential in predicting spatial variability in fruit quality.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Ron Shmuleviz1*, Alessandra Amato1, Pietro Previtali2, Elizabeth Green2, Luis Sanchez2, Maria Mar Alsina2, Nick Dokoozlian2, Giovanni Battista Tornielli1,3 and Marianna Fasoli1

1 Department of Biotechnology, University of Verona, 37134 Verona (VR), Italy
2 E. & J. Gallo Winery, Modesto, CA 95354, USA
3 Current address: Department of Agronomy, Food, Natural resources, Animals and  Environment, University of Padova, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

berry ripening, vegetation indices, gene expression analysis, sensors, precision viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Corvina and Corvinone grape berries grown in different areas and their aptitude to postharvest dehydration

The Valpolicella area (Veneto Region, Italy) is famous for its high quality wines: Amarone and Recioto, both obtained from partial post-harvest dehydrated red grapes.

Oxygen consumption by diferent oenological tanins in a model wine solution

INTRODUCTION: Oenological tannins are widely used in winemaking to improve some characteristics of wines [1] being the antioxidant properties probably one of the main reasons [2]. However, commercial tannins have different botanical sources and chemical composition [3] which probably determines different antioxidant potential. There are some few references about the antioxidant properties of commercial tannins [4] but none of them have really measured the direct oxygen consumption by them. The aim of this work was to measure the kinetics of oxygen consumption by different commercial tannins in order to determine their real capacities to protect wine against oxygen. MATERIAL AND METHODS: 4 different commercial tannins were used: T1: condensed tannin from grape seeds, T2: gallotannin from chinese gallnuts, T3: ellagitannin from oak and T4: tannin from quebracho containing condensed tannins and ellagitannins.

THE EFFECT OF COPPER ON THE PRODUCTION OF VARIETAL THIOLS DURING THE ALCOHOLIC FERMENTATION OF COLOMBARD AND GROS MANSENG GRAPE JUICES

Nowadays, the rapid growth of vineyards with organic practices and the use of copper as the only fun-gicide against downy mildew raises again the question of the effect of copper on varietal thiols in wine, especially 3-sulfanylhexan-1-ol (3SH) and its acetate (3SHA). A few decades ago, several works indicated that the use of copper in the vineyard had a negative effect on the content of varietal thiols in Sauvignon blanc wines [1, 2]. However, these studies only considered the concentration of the reduced form (RSH) of varietal thiols, without quantifying the oxidised ones. For this purpose, we proposed to monitor both reduced and oxidised forms of varietal thiols in wine under copper stress during alcoholic fermentation to have a more complete picture of the biological and chemical mechanisms.

Towards the understanding of wine distillation in the production of brandy de Jerez. Chemical and sensory characterization of two distillation methods: continuous and batch distillation

Brandy de Jerez (BJ) is a spirit drink made exclusively from spirits and wine distillates and is characterized by the use of casks for aging that previously contained Sherries. The quality and sensory complexity of BJ depend on the raw materials and some factors: grape variety, conditions during processing the wine and its distillation, as well as the aging in the cask. Therefore, the original compounds of the grapes from which it comes are of great interest being in most cases the Airén variety. Their relationship with the quality of the musts and the wines obtained from them has been studied (1) and varies each year of harvest depending on the weather conditions (2).

Amyndeon‐naoussa: the two faces of Xinomavro

Xinomavro is the most important indigenous red wine variety grown in Northern Greece. It participates in the production of several PGI wines in Macedonia while from 100% Xinomavro the PDO “Amyndeon” and “Naoussa” are produced. The viticultural area of Amyndeon lies in a plateau of 550 ‐700 m of altitude, in a semi‐continental climate with mostly deep sandy loamy soils derived from limestone and marl bedrocks while in Naoussa, Xinomavro is grown in a Mediterranean climate on more heavy textured soils, sandy clay loam to clay, derived from ophiolithic, limestone and marl bedrocks, in an altitude which varies from 150 to 400 m. Different soil, climate and viticultural technique interactions, result in great variability with respect to morphological, ampelographical and physiological characters of Xinomavro as well as in the characteristics of the wines produced.