terclim by ICS banner
IVES 9 IVES Conference Series 9 Spatial variability of grape berry maturation program at the molecular level 

Spatial variability of grape berry maturation program at the molecular level 

Abstract

The application of sensors in viticulture is a fast and efficient method to monitor grapevine vegetative, yield and quality parameters and determine their spatial intra-vineyard variability. Molecular analysis at the gene expression level can further contribute to the understanding of the observed variability by elucidating how pathways responsible for different grape quality traits behave in zones diverging for one or the other parameter. The intra-vineyard variability of a Cabernet Sauvignon vineyard was evaluated by a standard Normalized Difference Vegetation Index (NDVI) mapping approach, employing UAV platform, accompanied by detailed ground-truthing (e.g. vegetative, yield, and berry ripening compositional parameters) that was applied in 14 spots in the vineyard. Berries from different spots were additionally investigated by microarray gene expression analysis, performed at five time points from fruit set to full ripening. The relationships between NDVI and ground measurements were explored by correlation analysis and revealed high variability in the vineyard. Comparison between the transcriptome data of spots with the highest and lowest NDVI values unraveled 968 differentially expressed genes. Among them, were ripening-related genes, found to feature the low vigor spots, and genes involved in photosynthesis mechanisms that were prevalent in the high vigor spots. Spatial variability maps of the expression level of key berry ripening genes showed consistent patterns, aligned with the vineyard vigor map and with spatial maps generated for several vine and berry parameters. These insights suggest that berries from different vigor zones present distinct molecular maturation programs, hence, showing potential in predicting spatial variability in fruit quality.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Ron Shmuleviz1*, Alessandra Amato1, Pietro Previtali2, Elizabeth Green2, Luis Sanchez2, Maria Mar Alsina2, Nick Dokoozlian2, Giovanni Battista Tornielli1,3 and Marianna Fasoli1

1 Department of Biotechnology, University of Verona, 37134 Verona (VR), Italy
2 E. & J. Gallo Winery, Modesto, CA 95354, USA
3 Current address: Department of Agronomy, Food, Natural resources, Animals and  Environment, University of Padova, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

berry ripening, vegetation indices, gene expression analysis, sensors, precision viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Plastid genomics of Vitis vinifera L. for understanding the molecular basis of  grapevine (Vitis vinifera L.) domestication

The precise molecular mechanisms underlying the domestication of grapevine (Vitis vinifera L.) Are still not fully understood. In the recent years, next-generation sequencing (NGS) of plastid genomes has emerged as a powerful and increasingly effective tool for plant phylogenetics and evolution. To uncover the biological profile of the grapevine domestication process comprehensively, an investigation should encompass both the cultivated varieties (V. vinifera subsp. Vinifera) and their wild ancestors V. vinifera subsp. Sylvestris) across all potential sites of their distribution and domestication.

Sustainability as system innovation: sustainability as system innovation: a returnable system for glass wine bottles

Introduction increasing sustainability is essential and a societal challenge, requiring fundamental changes in behaviour and attitudes. This applies to both producers and consumers. For the wine industry in particular, such a change is a major challenge. An eip-agri research project is evaluating the introduction of a returnable glass system in the german wine industry as a key solution for increasing sustainability. Given the need for change associated with a returnable system, the project is theoretically grounded in systems innovation, as this approach provides solutions for complex, transformative change.

La sémantique liée à la notion de terroir : une objectivité pluridisciplinaire

It is not easy at first sight to give an exhaustive definition of the notion of terroir as it can be simplified or complicated at will. Thus the vagueness that surrounds this concept leaves the door open to various interpretations of the terroir. These tend towards a questionable level of objectivity because the fields they explore are not sufficient to explain the notion on their own, constituting only part of a whole.

Caratterizzazione varietale della CV. Vranac del Montenegro: primi risultati

Questo studio ha permesso di raccogliere alcune informazioni sul profilo chimico della cultivar Vranac coltivata in Montenegro. L’uva ha mostrato di raggiungere un buon accumulo zuccherino

Prototype development for the recovery of wine aromas from fermentation gases

Dealcoholised beverages are trendy. But this market segment is slowed down by flavour losses during dealcoholisation and by the reduced perception of flavours in the absence of alcohol.