The Douro region: wine and tourism

The relationship between grape production and leaf surface is a highly debated aspect in terms of the impact it may have on the composition and quality of grapes, especially in areas that focus their cultivation on high-quality wine. In many occasions, the limitation of the unitary production level in these areas is claimed to be the main factor for achieving high quality levels in the wine, forgetting the importance of the source-sink relationship and other environmental factors and management of the canopy. Taking this consideration into account, this work seeks to know the response of the vine as a whole, and the individual shoot as well, to the application of various alternatives of leaves and clusters removal, carried out in the phase immediately before veraison, in cv. Verdejo, in Spain.

In neutral grapes, it is known that most aroma compounds are present as non-volatile
precursors.

One of the main effects of global warming is an increase in the sugar concentration of grapes at harvest time, resulting in wines with a high alcohol content and an unbalanced structure. The fruit to leaf ratio is a key factor in determining the final sugar concentration, and training systems and management techniques can help to control this parameter.

One of the main aspects of Climate Change is the increase of temperatures during summer and grape maturity period. Physiological processes are influenced by these high temperatures and result in grapes with higher sugar concentration, less acidity and less anthocyanin content among other quality changes. One strategy to deal with the climate change effects is the implementation of late winter pruning to alter the effect of high temperatures during key periods by delays in maturity time.

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.