SSR analysis of some Vitis sylvestris (GMEL.) accessions of the Szigetköz and Fertő-hanság national park, Hungary

This study aims to evaluate the interest of using an under-vine cover crop as a sustainable management tool replacing herbicides or tillage to control weeds, evaluating its effects on yield and berry parameters in a semi-arid climate.  The performance of Trifolium fragiferum as an under-vine cover crop was evaluated in 2018 and 2019 in a Merlot vineyard in

Valpolicella is an Italian wine producing region, famous for the production of high-quality red wines. A distinctive characteristic of this region is the extensive use of post-harvest withering.

In the French AOCs, the production area of ​​the raw material can be subject to plot delimitation based on criteria of physical environment and use. On the other hand, many environmental zonings are developing and the AOCs are called upon include provisions relating to these concerns. Hedges, through their effects on local changes in the regional climate and on functional biodiversity, can impact the functioning of vines and orchards. It is for this reason that their consideration as a delimitation criterion is envisaged.

AIM Methyl salicylate (MeSA) has been reported as a potentially impactful compound in Verdicchio wines produced in central Italy. Lugana is another white wine produced in the north-east of Italy from a grape locally known as Trebbiano di Soave, sharing a very similar genetic background with Verdicchio. The aims of this study were evaluating MeSA occurrence in Lugana, assessing its aroma impact on white wines aroma and elucidating its biogenesis during vinification. METHODS Fifteen Lugana wines were analysed for methyl salycilate content in comparison with Verdicchio, Pinot grigio and Garganega wines. MeSA impact on white wine aroma was studied by means of triangular test, adding MeSA at different concentrations. Possible routes of MeSA formation by yeast were investigated by means of a high throughput assay in which S. cerevisiae cells were put in contact with precursor such as salicylic acid (esterification) or glycosidic extracts (glycosidase). Sub-fractions of Lugana glycosidic extracts were also obtained by HPLC fractionation, allowing further evaluation of precursors role.

We propose here a brand new large routine microbiological analysis method intended for oenology, in flow cytometry, using high performance equipment and triple selective cell staining, activated by fluorescence. The results and practical applications of the method are presented: Brettanomyces (Dekkera) Monitoring, fermentations monitoring, bottling and enological practices monitoring.The method allow a complete new microbiological tool for wine industry.The method has been accredited ISO 17025 in our laboratories.