Design of an indicator of vine vigor potential conferred by soil (vipos), using a fuzzy expert system

Uruguay had an average annual rainfall of 1200 mm characterized by a high monthly variability, which generates periods of water deficit and excess. The rational water management

Grapevines are susceptible to freezing damage at temperatures below -5°F during the winter season. Preventing winter injury to grapevines is a major challenge in many grape-producing regions. Conventional methods such as hilling-up soil over graft unions have been developed as winter protection methods for preventing vine loss. However, these practices have drawbacks such as soil erosion, vine damage and crown gall development.

Wine aroma analysis can be done by sensorial or instrumental analysis, the latter involving several me-thodologies based on olfactometric detection, electronic noses or gas chromatography. Gas Chromatography has been widely used for the study of the volatile composition of wines and depending on the detection system coupled to the chromatographic system, quantification and identification of individual compounds can be achieved.

The development and application of CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) technologies have revolutionized genome editing in plants due to its simplicity, high efficiency, and versatility. As an economically important fruit crop worldwide, grapevine genome editing using CRISPR/Cas technologies has also been reported these years. Here we introduce the development briefly of the most popular CRISPR/Cas9 system and also the state-of-the-art CRISPR technologies developed so far. Moreover, we summarize CRISPR/Cas9-mediated applications for gene functional study and trait improvement in grapevine.

Kékfrankos (Vitis vinifera L.) grapevines grafted on Teleki-Kober 5BB rootstock were submitted to water deficit under greenhouse conditions.