Terroir 2020 banner
IVES 9 IVES Conference Series 9 Genetic diversity of Oenococcus oeni strains isolated from Yinchuan wine region in the East of Helan Mountain, China

Genetic diversity of Oenococcus oeni strains isolated from Yinchuan wine region in the East of Helan Mountain, China

Abstract

Aim: This study aimed to isolate Oenococcus oeni in red wines from Yinchuan wine region in the East of Helan Mountain, China, and analysis their genetic diversity.

Methods and Results: Oenococcus oeni strains were isolated from Cabernet Sauvignon and Cabernet Gernischt wines of four representative wineries in 2016. Total 207 O. oeni strains were isolated and identified by species-specific PCR. Following that, 206 amplified fragment length polymorphism (AFLP) genotypes were detected, with the similarity coefficients ranging between 63% – 97%. Based on the UPGMA, two major phylogroups were deciphered at 81% similarity level. Interestingly, the strains in different phylogroups were isolated from wines of different cultivars. In addition, strains from the same winery formed a unique cluster.

Conclusions: 

Our results indicate there is an obvious genetic relationship of O. oeni with grape cultivars and their origins. Our results also support the fact that O. oeni is an important factor related to the wine terroir. 

Significance and Impact of the Study: The Chinese wine industry has steadily grown in recent years. However, limited development and application of indigenous O. oeni strains would lead to homogeneity in wine quality. The outcome of this study would lay down the theoretical foundation for the development of indigenous O. oeni strains with regional characteristics.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Kan Shi1,3 ,4, Huawei Gu2, Dongliang Yu5, Shuwen Liu1,3,4*

1College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China
2College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
3Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, Shaanxi 712100, China
4Heyang Experimental and Demonstrational Stations for Grape, Weinan, Shaanxi 715300, China
5Qinhuangdao Chateau Kings Global Co., Ltd, Changli, Hebei 066600, China

Contact the author

Keywords

Wine, Oenococcus oeni, genetic diversity, AFLP

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Effect of auxin treatment on delaying maturation of grape cultivars in the Valpolicella viticultural area

The temperature increase related to ongoing climate changes is causing a progressive anticipation of the ripening time, negatively affecting grape quality at harvest.

Effect of rising atmospheric CO2 levels on grapevine yield and composition by the middle of the 21st century: what can we learn from the VineyardFACE?

Atmospheric CO2 levels have been rising continuously since the industrial revolution, affecting crop physiology, yield and quality of harvest products, and grapevine is no exception [1]. Most of previously reported studies used potted plants in controlled environments, and explored grapevine response to relatively high CO2 levels, 700 ppm or more. The vineyardFACE, established in Geisenheim in 2012, uses a free air carbon dioxide enrichment (FACE) system to simulate a moderate (ambient +20%) increase in atmospheric CO2 in a vineyard planted with cvs. Cabernet-Sauvignon and Riesling grafted on rootstock 161-49 Couderc and SO4, respectively.

Characterization of 25 white grape varieties from the variety collection of ICVV (D.O.Ca.Rioja, Spain)

The effects of climate change produce an increase in sugar concentration and a decrease in acidity, without reaching the optimum grape phenolic maturity [1]. The aim of this work was to characterize 25 white grape varieties

Haplotype-Resolved genome assembly of the Microvine

Developing a tractable genetic engineering and gene editing system is an essential tool for grapevine. We initiated a plant transformation and biotechnology program at Oregon State University using the grape microvine system (V. vinifera) in 2018 to interrogate gene-to-trait relationships using traditional genetic engineering and gene editing. The microvine model is also used for nanomaterial-assisted RNP, DNA, and RNA delivery. Most reference genomes and annotations for grapevine are collapsed assemblies of homologous chromosomes and do not represent the specific microvine cultivar ‘043023V004’ under study at our institution.

Mitigation of retronasal smoke flavor carryover in the sensory analysis of smoke affected wines

With the steady rise in wildfire occurrence in wine regions around the world, there are quality issues beginning to face the wine industry. These fires produce clouds of smoke which have the ability to carry organic molecules across vast distances that can be absorbed by grapes. When these compounds make their way into the final wine, unpleasant smokey and burnt flavors are present, along with a lasting ashy finish. Along with the volatile compounds carried by smoke, once incorporated into the fruit these compounds become bound to sugars, forming glycosidic compounds.