Terroir 2020 banner
IVES 9 IVES Conference Series 9 Genetic diversity of Oenococcus oeni strains isolated from Yinchuan wine region in the East of Helan Mountain, China

Genetic diversity of Oenococcus oeni strains isolated from Yinchuan wine region in the East of Helan Mountain, China

Abstract

Aim: This study aimed to isolate Oenococcus oeni in red wines from Yinchuan wine region in the East of Helan Mountain, China, and analysis their genetic diversity.

Methods and Results: Oenococcus oeni strains were isolated from Cabernet Sauvignon and Cabernet Gernischt wines of four representative wineries in 2016. Total 207 O. oeni strains were isolated and identified by species-specific PCR. Following that, 206 amplified fragment length polymorphism (AFLP) genotypes were detected, with the similarity coefficients ranging between 63% – 97%. Based on the UPGMA, two major phylogroups were deciphered at 81% similarity level. Interestingly, the strains in different phylogroups were isolated from wines of different cultivars. In addition, strains from the same winery formed a unique cluster.

Conclusions: 

Our results indicate there is an obvious genetic relationship of O. oeni with grape cultivars and their origins. Our results also support the fact that O. oeni is an important factor related to the wine terroir. 

Significance and Impact of the Study: The Chinese wine industry has steadily grown in recent years. However, limited development and application of indigenous O. oeni strains would lead to homogeneity in wine quality. The outcome of this study would lay down the theoretical foundation for the development of indigenous O. oeni strains with regional characteristics.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Kan Shi1,3 ,4, Huawei Gu2, Dongliang Yu5, Shuwen Liu1,3,4*

1College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China
2College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
3Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, Shaanxi 712100, China
4Heyang Experimental and Demonstrational Stations for Grape, Weinan, Shaanxi 715300, China
5Qinhuangdao Chateau Kings Global Co., Ltd, Changli, Hebei 066600, China

Contact the author

Keywords

Wine, Oenococcus oeni, genetic diversity, AFLP

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Downy mildew, caused by the oomycete Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni, is one of the most destructive grapevine diseases mostly affecting Vitis vinifera L. and impacting on viticulture. The pathogen invasion can induce in grapevine multiple defense reactions, first PAMP-Triggered Immunity and secondly Effector-Triggered Immunity. Plasmopara viticola can overcome these defense mechanisms through the secretion of effectors, such as RxLR, into the plant cells, making it easier for the oomycete to infect grapevines. Currently, the use of chemical pesticides remains the most effective way to control the pathogen with severe negative side effects on the environment and animal health.

When organic chemistry contributes to the understanding of metabolism mechanisms

Many compounds of interest in wine are difficult to analyze since they are present in very small quantities or they are unstable. The need for reliable data led scientists to develop complex method in order to overcome the analytical difficulties and provide accurate quantitative data for grape or wine characterization.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Deciphering the color of rosé wines using polyphenol targeted metabolomics

The color of rosés wines is extremely diverse and a key element in their marketing. It is due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making.

Wine shaking during transportation: influence on the analytical and sensory parameters of wine

According to OIV reports, annual world wine consumption fluctuated around 240-245 mln hL over the past decade. The general market globalization has led to the situation when almost half of the consumed wine is exported to other countries. Of this volume, more than 60 mln hL are bottled still and sparkling wines.