Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Effect of application of kaolin and pinolene on grape berry cell death, berry shrinkage, and ethanol accumulation

Effect of application of kaolin and pinolene on grape berry cell death, berry shrinkage, and ethanol accumulation

Abstract

AIM: Cell death in Vitis vinifera L. berries late in ripening and berry shrinkage (loss of mass) can decrease yield and reduce grape quality in cultivars such as Cabernet Sauvignon, Merlot, and especially Shiraz. Techniques to ameliorate effects of cell death and berry shrinkage are limited. Pinolene and kaolin are two types of film-forming antitranspirants applied to plants to reduce water loss. If these antitranspirants create a water impermeable coating, they may also restrict gas exchange, exacerbating hypoxia associated with cell death in grape berries. This study aimed to identify the effects on berry physiology during ripening of kaolin and pinolene coatings on Shiraz and Grenache bunches.

METHODS: Kaolin (6% w/w), pinolene (1% w/w) and water (control) were sprayed on Shiraz and Grenache bunches (2019-2020, Waite campus University of Adelaide) during ripening every 7 to 15 days. Change in berry mass, cell vitality, internal oxygen concentration, ethanol accumulation and bunch and canopy temperature were recorded.

RESULTS: Grenache berries had almost no shrinkage and no cell death during development contrasting to continuous decline in berry mass and cell vitality in Shiraz berries from 85 days after anthesis. Kaolin had no effects on berry properties. Pinolene reduced loss of berry mass in Shiraz and slightly increased berry mass in Grenache, leading to lower sugar concentrations in both cultivars. There was no effect of pinolene on berry oxygen concentration or cell vitality since both declined similarly to controls. There was an exponential increase in berry ethanol concentration with increasing mean daily temperature. Berry ethanol concentration for Grenache was much lower than for Shiraz under similar temperature conditions. There was no effect of treatments on berry ethanol concentrations.

CONCLUSIONS

Pinolene decreased berry shrinkage and prevented high sugar concentration presumably by reducing transpiration without impacting sugar content. It was surprising that this compound could decrease water loss without apparently affecting internal oxygen concentration in the berry. Ethanol accumulation during berry ripening could be a causative factor of cell death or is closely associated with it. Temperature may decrease berry vitality by accelerating respiration which leads to anoxia and high ethanol production.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Lishi Cai

School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia,Apriadi Situmorang School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia Steve Tyerman School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia

Contact the author

Keywords

shiraz, grenache, berry cell death, kaolin, pinolene (di-1-p-menthene), ethanol, oxygen

Citation

Related articles…

Grapevine sugar concentration model in the Douro Superior, Portugal

Increasingly warm and dry climate conditions are challenging the viticulture and winemaking sector. Digital technologies and crop modelling bear the promise to provide practical answers to those challenges. As viticultural activities strongly depend on harvest date, its early prediction is particularly important, since the success of winemaking practices largely depends upon this key event, which should be based on an accurate and advanced plan of the annual cycle. Herein, we demonstrate the creation of modelling tools to assess grape ripeness, through sugar concentration monitoring. The study area, the Portuguese Côa valley wine region, represents an important terroir in the “Douro Superior” subregion. Two varieties (cv. Touriga Nacional and Touriga Franca) grown in five locations across the Côa Region were considered. Sugar accumulation in grapes, with concentrations between 170 and 230 g l-1, was used from 2014 to 2020 as an indicator of technological maturity conditioned by meteorological factors. The climatic time series were retrieved from the EU Copernicus Service, while sugar data were collected by a non-profit organization, ADVID, and by Sogrape, a leading wine company. The software for calibrating and validating this model framework was the Phenology Modeling Platform (PMP), version 5.5, using Sigmoid and growing degree-day (GDD) models for predictions. The performance was assessed through two metrics: Roots Mean Square Error (RMSE) and efficiency coefficient (EFF), while validation was undertaken using leave-one-out cross-validation. Our findings demonstrate that sugar content is mainly dependent on temperature and air humidity. The models achieved a performance of 0.65

Longevity and moderate wine consumption – can guidelines provide practical advice?

Conflicting messages about the consumption of alcoholic beverages – including wine – continue to dominate the media, causing increasing uncertainty among consumers and health professionals.

Breeding grapevines for disease and low temperature tolerance: the U.S. perspective

Most grape scion cultivars grown around the world are derived from a single species, Vitis vinifera. Yet, the proportion of interspecific hybrids is increasing for a variety of reasons, including resistance to abiotic stresses such as low temperatures; societal, economic and environmental pressures to reduce pesticide usage; and to add a greater range of flavors to new table grape cultivars.

Preliminary characterisation of mannoproteins from different wine yeast strains and impact on wine properties

Mannoproteins (MPs) are released from the yeast cell wall during alcoholic fermentation and aging on the lees, and influence aspects of wine quality such as haze formation and colour stability. Yet, as this is a slow process with microbiological and sensory risks, the exogenous addition of extracted MPs poses an efficient alternative. While Saccharomyces cerevisiae has long been studied as a prominent source for MPs extraction, their structure and composition greatly differ between yeast species. This may influence their behaviour in the wine matrix and subsequent impact on wine properties. However, although wine yeast species other than S. cerevisiae possibly present an untapped source of MPs, they are still ill-characterised in terms of chemical composition and influence on wine.

Influence of vine spacing on water status, productivity, yield and must composition in Tempranillo grapevine under Duero Valley zone conditions

L’objectif de cette étude est analyser l’influence de la densité de plantation sur l’état hydrique (potentiel hydrique), le comportement productif (matière sèche et rendement) et la expression qualitative (poids de baie, degrée Brix, pH, acidité totale, concentration polyphénolique) de la varieté Tempranillo dans la Vallée du Douro, à l’A.O. Cigales.