Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 A microwave digestion ICP-MS method for grapevine bark elemental profiling

A microwave digestion ICP-MS method for grapevine bark elemental profiling

Abstract

Aim: A rapid and reproducible microwave (MW)-assisted acid digestion protocol was developed to determine the elemental composition of grapevine bark samples using ICP-MS. A representative grapevine bark sample and a similar matrix Certified Reference Material (CRM) were used for method optimisation. The method was subsequently applied to a set of New Zealand vineyard grapevine bark samples consisting of seven different grape varieties.

Methods: A homogenous bark sample and a CRM (ERMCD281) were treated with 16 different acid combinations and microwave digestion settings prior to ICP-MS analysis. 54 chemical elements were measured in the samples. Calibration standards were prepared in matrix matched solutions from single elements standards (Inorganic Ventures, USA).

Results: The acid digestion combination of HNO3, H2O2, and HCl with a MW digestion of 15 minutes was shown to give optimal results. 48 elements could be measured in a representative grapevine bark sample using this procedure and 27 elements in a reference CRM sample. Ca was the most abundant element present in all grape variety bark samples.

Conclusions

A method was developed and validated for an MW digestion of grapevine bark samples using ICP-MS. The application of this new method showed that bark from different grape varieties varies in elemental composition within a vineyard site.

Acknowledgments

The authors wish to thank the Bragato Research Institute, New Zealand Winegrowers, and the Ministry of Business, Industry, and Employment (MBIE), for funding this work.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Alexandra Lowrey 

University of Auckland, New Zealand,Bruno FEDRIZZI, University of Auckland Rebecca JELLEY, University of Auckland Stuart MORROW, University of Auckland

Contact the author

Keywords

icp-ms, grapevine bark, trace elements, microwave digestion

Citation

Related articles…

Effect of power ultrasound treatment on free and glycosidically-bound volatile compounds and the sensorial profile of red wines

AIM Aiming to explore the possibility of shortening red winemaking maceration times (1,2), this study presents the effect of the application of high-power ultrasounds to crushed grapes, at winery-scale, on the content of varietal volatile compounds (free and glycosidically-bound) in musts and on the overall aroma of wines.

Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Vineyards are home to a myriad of microorganisms that interact with each other and with the vines. Some microorganisms are plant pathogens, such as the oomycete Plasmopara viticola, causing grapevine downy mildew. Others have a positive effect on vine health, such as disease biocontrol agents. These beneficial plant-microbe and microbe-microbe interactions have gained more attention in recent years because they could represent an alternative to the use of fungicides in viticulture.

Additives od aids? Evaluation of aroma compounds release from oenological tannins of different botanical origins.

Oenological tannins are products extracted from various botanical sources, such as mimosa,
acacia, oak gall, quebracho, chestnut and tara. The polyphenolic component is obtained through a solid-liquid extraction also using specific solvents, then removed by evaporation or freeze-drying. Tannins are employed in two phases of winemaking, during the pre-fermentative phase or during fining with different purposes such as modulate antioxidant activity, colour stabilization, bacteriostatic activity, protein stabilization and modulation of sensory properties. To date, the current regulatory framework is not very clear. In fact, the Codex Alimentarius classifies commercial tannins as “food additives” but also as
“processing aids”. The main distinction is that “additives” have a technological function in the final food, whereas “processing aids” do not. In this sense, oenological tannins, despite the technological treatments, could contain aromatic compounds of the botanical species they belong to and release them to the wine.

FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.

Effects of the biodynamic preparations 500 and 501 on vine and berry physiology, pedology and the soil microbiome

In the pursuit of increasing sustainability, climate change resiliency and independence of synthetic pesticides in agriculture, the interest of consumers and producers in organic and biodynamic farming is steadily increasing. This is in particular the case for the vitivinicultural industry in Europe, where more and more producers are converting from organic to biodynamic farming. However, clear scientific evidence showing that biodynamic farming improves vine physiology, vine stress resilience, berry or wine quality, or is more sustainable for the environment is still lacking although this issue has been addressed by several research teams worldwide.