Macrowine 2021
IVES 9 IVES Conference Series 9 Optimised extraction and preliminary characterisation of mannoproteins from non-Saccharomyces wine yeasts

Optimised extraction and preliminary characterisation of mannoproteins from non-Saccharomyces wine yeasts

Abstract

The use of non-Saccharomyces yeast species for the improvement of wine technological and oenological properties is a topic that has gained much interest in recent years [1]. Their application as co-starter cultures sequential to the inoculation of Saccharomyces cerevisiae and in aging on the lees has been shown to improve aspects such as protein stability and mouthfeel [2]. These contributions have frequently been associated with higher levels of polysaccharides, specifically the cell wall-derived mannoprotein [3]. Furthermore, mannoprotein structure and composition has been shown to vary between yeast strains, which in turn may influence their behaviour in the wine matrix [4-6]. However, non-Saccharomyces yeasts are typically weak fermentors and are frequently out-competed in the fermentation medium. An alternative strategy to their use as co-starter cultures is the isolation of the compound of interest for exogenous application to wine [7]. Indeed, the addition of exogenous mannoprotein-containing products derived from the cell wall of the wine yeast S. cerevisiae is a fairly common winemaking practice [8]. Nevertheless, the extraction of mannoproteins from non-Saccharomyces yeasts has not yet been well described. AIM: This study aimed to optimise the extraction of mannoproteins from four non-Saccharomyces strains, and to perform a preliminary investigation into the compositional differences of the mannoproteins obtained from the different species.

METHODS: Four non-Saccharomyces wine strains, Saccharomyces cerevisiaeSaccharomyces boulardiiMetschnikowia fructicola and Torulaspora delbrueckii, were exposed to combined methods with varied parameters of ultrasound and enzymatic extraction with β-glucanase to optimise mannoprotein yield. Colorimetric assays were used to quantify protein and carbohydrate concentrations in the extracts.

RESULTS: Yeast cells subjected to 4 min of ultrasound treatment applied at 80% of the maximum amplitude with a 50% duty cycle, followed by an enzymatic treatment of 4000 U lyticase per g dry cells weight, showed the highest yield of mannoproteins from all species. Furthermore, preliminary evaluation of the obtained extracts revealed differences in carbohydrate/protein ratios between species and with increased enzyme incubation time, as demonstrated by the higher ratios obtained for T. Delbrueckii and S. cerevisiae after almost all treatments, in comparison to M. fructicola and S. boulardii.

CONCLUSIONS: The results obtained in this study form an important step towards further characterisation of extraction treatment impact and yeast species effect on the extracted mannoproteins. Their impact on the carbohydrate/protein ratio in particular is an important factor to consider for applications such as wine protein haze reduction and tartrate stabilisation, and requires more in-depth investigation of isolated mannoproteins.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Carla Snyman, Benoit DIVOL, Matteo MARANGON, Julie MEKOUE NGUELA, Nathalie SCIECZKOWSKI

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa, South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa, Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale Dell’Università, 16, 35020, Legnaro, Padova, Italy, Lallemand SAS, 19 rue des briquetiers, BP 59, 31702 Blagnac, France, Lallemand SAS, 19 rue des briquetiers, BP 59, 31702 Blagnac, France

Contact the author

Keywords

mannoprotein; yeast; non-saccharomyces; extraction; wine; ultrasound; β-glucanase

Citation

Related articles…

Discrimination of monovarietal Italian red wines using derivative voltammetry

Identification of specific analytical fingerprints associated to grape variety, origin, or vintage is of great interest for wine producers, regulatory agencies, and consumers. However, assessing such varietal fingerprint is complex, time consuming, and requires expensive analytical techniques. Voltammetry is a fast, cheap, and user-friendly analytical tool that has been used to investigate and measure wine phenolics.

Potential use of the yeast Starmerella bacillaris as a sustainable biocontrol agent against gray mold disease in viticulture

Pest biocontrol strategies are gaining attention as eco-friendly alternatives to the use of synthetic pesticides, including in viticulture.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Artificial intelligence-driven classification method of grapevine phenology using conventional RGB imaging

The phenological stage of the grapevine (Vitis vinifera L.) represents a fundamental element in vineyard management, since it determines key practices such as fertilization, irrigation, phytosanitary interventions and optimal harvest time (Mullins et al., 1992).

Flanan-3-ol compositional changes in red grape berries (Vitis vinifera L. cv Cabernet franc) from two terroirs of the Loire Valley (France)

La quantité et la qualité des flavonoïdes sont des éléments importants de la qualité de la baie. En particulier, les tannins contribuent de manière essentielle aux propriétés spécifiques des vins rouges telles que la couleur, l’astringence et l’amertume. Cependant, leur synthèse et leurs propriétés sont encore mal connues. Ainsi, la

Macrowine 2021
IVES 9 IVES Conference Series 9 Optimised extraction and preliminary characterisation of mannoproteins from non-Saccharomyces wine yeasts

Optimised extraction and preliminary characterisation of mannoproteins from non-Saccharomyces wine yeasts

Abstract

Content of the article

References

Section for all references

DOI:

Publication date: September 3, 2021

Issue: (ex: Issue: Terclim 2023)

Type: typeofpublication

Authors

author1, author2, author3

Presenting author

Description

List of affiliations ¹ ² ³

Contact the author

Email address (with mailto: link)

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

Citation

Related articles…

Innovations on red winemaking process by ultrasound technology

High power ultrasound has been recently recognized one of the most promising technologies in winemaking processes, especially after the recent OIV resolution, concerning the application of ultrasounds on crushed grapes to promote the extraction of skin compounds.

Using the fraction of transpirable soil water to estimate grapevine leaf water potential: comparing the classical statistical regression approach to machine learning algorithms

Weather uncertainty is forcing Mediterranean winegrowers to adopt new irrigation strategies to cope with water scarcity while ensuring a sustainable yield and improved berry and wine quality standards. Therefore, more accurate and high-resolution monitoring of soil water content and vine water status is a major concern. Leaf water potential measured at pre-dawn (PD) is considered to be in equilibrium with soil water potential and is highly correlated with soil water content at the soil depth where roots extract water.

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.

Drought tolerance of varieties in semi-arid areas: can the behavior of Tempranillo be improved by varieties of its own lineage?

Tempranillo is the most widely grown red grapevine variety in Spain, currently representing 42% of the total number of red varieties and 21% of the total vineyard area. Due to the economic importance that this variety represents in Spanish viticulture, in some areas where it is traditionally grown, there is a special concern about the viability of the future growing of this variety is being compromised by the climate change effects.

Improving stilbenes in vitis Labrusca L. Grapes through methyl jasmonate applications

Grapes (Vitis sp.) are considered a major source of phenolic compounds such as flavonols, anthocyanins and stilbenes. Studies related to the beneficial effects of these compounds on health have encouraged research aimed at increasing their concentration in fruits. On this behalf, several plant growth regulators such as jasmonic acid and its volatile ester, methyl-jasmonate (MeJa), have demonstrated promising results in many fruits. However, Brazilian subtropical climate might interfere on treatment response. The present study aims to evaluate the application of MeJa in the pre-harvest period in Concord and Isabel Precoce grapes (Vitis labrusca L.).